ParkinsonFranceV1, Hal, Checkpoint, bibRecord, 000374

Strategies for proteomic analysis of cerebral tissue and biological fluids in Parkinson's disease

Identifieur interne : 000374 ( Hal/Checkpoint ); précédent : 000373; suivant : 000375

Strategies for proteomic analysis of cerebral tissue and biological fluids in Parkinson's disease

Auteurs : Affif Zaccaria [France]

Source :

RBID : Hal:tel-00820156

Descripteurs français

mix :
- Empreinte tissulaire, Maladie de Parkinson, Nanoparticules, Proteomique.

English descriptors

mix :
- Nanoparticles, Parkinson's disease, Proteomic, Tissue imprint.

Abstract

Proteomics analysis of pathological brain tissue in Parkinson's disease (PD) is of great importance to understand the molecular aetiology of degeneration and to develop curative treatments. To date, published studies have been restricted to the analysis of human post-mortem tissue samples, frequently derived from advanced disease stage and potentially altered by several factors. In this project we took advantage of the temporary access to PD patient's brain during electrode implantation to obtain in vivo molecular information from cerebral tissue at earlier stage of the disease. We further developed a dedicated tool to improve our tissue harvesting approach, and validated its efficiency and non-lesion effects in vivo in monkeys. This work opens the way to the proteomic analysis of fresh human brain samples to elucidate molecular causes of degeneration in PD. However such tissue investigation approach remains invasive and cannot be used in routine clinical screening for PD diagnosis. Proteomics analysis of cerebrospinal fluid (CSF) constitutes a promising alternative to identify neuropathological diagnosis markers. For this purpose, we developed a nanoparticle-assisted strategy enabling the enrichment of CSF proteins detection by mass spectrometry. Reproducibility and high throughput potentiality of our approach demonstrate its compatibility with clinical proteomics for PD diagnosis biomarker research in CSF. We also demonstrated the interest of this NP strategy for plasma and red blood cells proteome analysis. Finally, we evaluated the ability to use these NPs for in vivo protein harvesting in blood.

Url:

https://tel.archives-ouvertes.fr/tel-00820156

Links toward previous steps (curation, corpus...)

to stream Hal, to step Corpus: 000A36
to stream Hal, to step Curation: 000A36

Links to Exploration step

Hal:tel-00820156

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Strategies for proteomic analysis of cerebral tissue and biological fluids in Parkinson's disease</title>
<title xml:lang="fr">Nouvelles stratégies pour l'analyse protéomique du tissu cérébral et des fluides biologiques dans la maladie de Parkinson</title>
<author><name sortKey="Zaccaria, Affif" sort="Zaccaria, Affif" uniqKey="Zaccaria A" first="Affif" last="Zaccaria">Affif Zaccaria</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-86972" status="VALID">  <orgName>Grenoble Institut des Neurosciences</orgName>
  <orgName type="acronym">GIN</orgName>
  <desc>    <address>      <addrLine>UJF - Site Santé La Tronche - BP 170 - 38042 Grenoble Cedex 9</addrLine>
      <country key="FR"></country>
    </address>
    <ref type="url">http://neurosciences.ujf-grenoble.fr/</ref>
  </desc>
  <listRelation>    <relation active="#struct-51016" type="direct"></relation>
    <relation name="DSV/IRTSV" active="#struct-300016" type="direct"></relation>
    <relation active="#struct-300071" type="direct"></relation>
    <relation name="U836" active="#struct-303623" type="direct"></relation>
  </listRelation>
<tutelles><tutelle active="#struct-51016" type="direct"><org type="institution" xml:id="struct-51016" status="OLD">  <idno type="IdRef">026404796</idno>
  <orgName>Université Joseph Fourier - Grenoble 1</orgName>
  <orgName type="acronym">UJF</orgName>
  <date type="end">2015-12-31</date>
  <desc>    <address>      <addrLine>BP 53 - 38041 Grenoble Cedex 9</addrLine>
      <country key="FR"></country>
    </address>
    <ref type="url">http://www.ujf-grenoble.fr/</ref>
  </desc>
</org>
</tutelle>
<tutelle name="DSV/IRTSV" active="#struct-300016" type="direct"><org type="institution" xml:id="struct-300016" status="VALID">  <orgName>Commissariat à l'énergie atomique et aux énergies alternatives</orgName>
  <orgName type="acronym">CEA</orgName>
  <desc>    <address>      <addrLine>Centre de SaclayCentre de GrenobleCentre de Cadaracheetc</addrLine>
      <country key="FR"></country>
    </address>
    <ref type="url">http://www.cea.fr/</ref>
  </desc>
</org>
</tutelle>
<tutelle active="#struct-300071" type="direct"><org type="institution" xml:id="struct-300071" status="VALID">  <orgName>CHU Grenoble</orgName>
  <desc>    <address>      <country key="FR"></country>
    </address>
  </desc>
</org>
</tutelle>
<tutelle name="U836" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID">  <idno type="IdRef">026388278</idno>
  <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
  <orgName type="acronym">INSERM</orgName>
  <desc>    <address>      <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
      <country key="FR"></country>
    </address>
    <ref type="url">http://www.inserm.fr</ref>
  </desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Grenoble</settlement>
<region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
</placeName>
<orgName type="university">Université Joseph Fourier</orgName>
<orgName type="institution" wicri:auto="newGroup">Université de Grenoble</orgName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">HAL</idno>
<idno type="RBID">Hal:tel-00820156</idno>
<idno type="halId">tel-00820156</idno>
<idno type="halUri">https://tel.archives-ouvertes.fr/tel-00820156</idno>
<idno type="url">https://tel.archives-ouvertes.fr/tel-00820156</idno>
<date when="2013-03-15">2013-03-15</date>
<idno type="wicri:Area/Hal/Corpus">000A36</idno>
<idno type="wicri:Area/Hal/Curation">000A36</idno>
<idno type="wicri:Area/Hal/Checkpoint">000374</idno>
<idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000374</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Strategies for proteomic analysis of cerebral tissue and biological fluids in Parkinson's disease</title>
<title xml:lang="fr">Nouvelles stratégies pour l'analyse protéomique du tissu cérébral et des fluides biologiques dans la maladie de Parkinson</title>
<author><name sortKey="Zaccaria, Affif" sort="Zaccaria, Affif" uniqKey="Zaccaria A" first="Affif" last="Zaccaria">Affif Zaccaria</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-86972" status="VALID">  <orgName>Grenoble Institut des Neurosciences</orgName>
  <orgName type="acronym">GIN</orgName>
  <desc>    <address>      <addrLine>UJF - Site Santé La Tronche - BP 170 - 38042 Grenoble Cedex 9</addrLine>
      <country key="FR"></country>
    </address>
    <ref type="url">http://neurosciences.ujf-grenoble.fr/</ref>
  </desc>
  <listRelation>    <relation active="#struct-51016" type="direct"></relation>
    <relation name="DSV/IRTSV" active="#struct-300016" type="direct"></relation>
    <relation active="#struct-300071" type="direct"></relation>
    <relation name="U836" active="#struct-303623" type="direct"></relation>
  </listRelation>
<tutelles><tutelle active="#struct-51016" type="direct"><org type="institution" xml:id="struct-51016" status="OLD">  <idno type="IdRef">026404796</idno>
  <orgName>Université Joseph Fourier - Grenoble 1</orgName>
  <orgName type="acronym">UJF</orgName>
  <date type="end">2015-12-31</date>
  <desc>    <address>      <addrLine>BP 53 - 38041 Grenoble Cedex 9</addrLine>
      <country key="FR"></country>
    </address>
    <ref type="url">http://www.ujf-grenoble.fr/</ref>
  </desc>
</org>
</tutelle>
<tutelle name="DSV/IRTSV" active="#struct-300016" type="direct"><org type="institution" xml:id="struct-300016" status="VALID">  <orgName>Commissariat à l'énergie atomique et aux énergies alternatives</orgName>
  <orgName type="acronym">CEA</orgName>
  <desc>    <address>      <addrLine>Centre de SaclayCentre de GrenobleCentre de Cadaracheetc</addrLine>
      <country key="FR"></country>
    </address>
    <ref type="url">http://www.cea.fr/</ref>
  </desc>
</org>
</tutelle>
<tutelle active="#struct-300071" type="direct"><org type="institution" xml:id="struct-300071" status="VALID">  <orgName>CHU Grenoble</orgName>
  <desc>    <address>      <country key="FR"></country>
    </address>
  </desc>
</org>
</tutelle>
<tutelle name="U836" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID">  <idno type="IdRef">026388278</idno>
  <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
  <orgName type="acronym">INSERM</orgName>
  <desc>    <address>      <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
      <country key="FR"></country>
    </address>
    <ref type="url">http://www.inserm.fr</ref>
  </desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Grenoble</settlement>
<region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
</placeName>
<orgName type="university">Université Joseph Fourier</orgName>
<orgName type="institution" wicri:auto="newGroup">Université de Grenoble</orgName>
</affiliation>
</author>
</analytic>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>Nanoparticles</term>
<term>Parkinson's disease</term>
<term>Proteomic</term>
<term>Tissue imprint</term>
</keywords>
<keywords scheme="mix" xml:lang="fr"><term>Empreinte tissulaire</term>
<term>Maladie de Parkinson</term>
<term>Nanoparticules</term>
<term>Proteomique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Proteomics analysis of pathological brain tissue in Parkinson's disease (PD) is of great importance to understand the molecular aetiology of degeneration and to develop curative treatments. To date, published studies have been restricted to the analysis of human post-mortem tissue samples, frequently derived from advanced disease stage and potentially altered by several factors. In this project we took advantage of the temporary access to PD patient's brain during electrode implantation to obtain in vivo molecular information from cerebral tissue at earlier stage of the disease. We further developed a dedicated tool to improve our tissue harvesting approach, and validated its efficiency and non-lesion effects in vivo in monkeys. This work opens the way to the proteomic analysis of fresh human brain samples to elucidate molecular causes of degeneration in PD. However such tissue investigation approach remains invasive and cannot be used in routine clinical screening for PD diagnosis. Proteomics analysis of cerebrospinal fluid (CSF) constitutes a promising alternative to identify neuropathological diagnosis markers. For this purpose, we developed a nanoparticle-assisted strategy enabling the enrichment of CSF proteins detection by mass spectrometry. Reproducibility and high throughput potentiality of our approach demonstrate its compatibility with clinical proteomics for PD diagnosis biomarker research in CSF. We also demonstrated the interest of this NP strategy for plasma and red blood cells proteome analysis. Finally, we evaluated the ability to use these NPs for in vivo protein harvesting in blood.</div>
</front>
</TEI>
<hal api="V3">          <titleStmt>            <title xml:lang="en">Strategies for proteomic analysis of cerebral tissue and biological fluids in Parkinson's disease</title>
            <title xml:lang="fr">Nouvelles stratégies pour l'analyse protéomique du tissu cérébral et des fluides biologiques dans la maladie de Parkinson</title>
            <author role="aut">              <persName>                <forename type="first">Affif</forename>
                <forename type="middle">Zaccaria</forename>
                <surname>Zaccaria</surname>
              </persName>
              <idno type="halauthorid">1175555</idno>
              <idno type="IdRef">http://www.idref.fr/16908986X</idno>
              <affiliation ref="#struct-86972"></affiliation>
              <affiliation ref="#struct-421139"></affiliation>
            </author>
            <editor role="depositor">              <persName>                <forename>ABES</forename>
                <surname>STAR</surname>
              </persName>
              <email type="md5">f5aa7f563b02bb6adbba7496989af39a</email>
              <email type="domain">abes.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>            <edition n="v1" type="current">              <date type="whenSubmitted">2013-05-03 11:52:10</date>
              <date type="whenModified">2015-06-11 03:43:22</date>
              <date type="whenReleased">2013-05-03 13:17:05</date>
              <date type="whenProduced">2013-03-15</date>
              <date type="whenEndEmbargoed">2013-05-03</date>
              <ref type="file" target="https://tel.archives-ouvertes.fr/tel-00820156/document">                <date notBefore="2013-05-03"></date>
              </ref>
              <ref type="file" subtype="author" n="1" target="https://tel.archives-ouvertes.fr/tel-00820156/file/22438_ZACCARIA_2013_archivage.pdf">                <date notBefore="2013-05-03"></date>
              </ref>
            </edition>
            <respStmt>              <resp>contributor</resp>
              <name key="131274">                <persName>                  <forename>ABES</forename>
                  <surname>STAR</surname>
                </persName>
                <email type="md5">f5aa7f563b02bb6adbba7496989af39a</email>
                <email type="domain">abes.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>            <distributor>CCSD</distributor>
            <idno type="halId">tel-00820156</idno>
            <idno type="halUri">https://tel.archives-ouvertes.fr/tel-00820156</idno>
            <idno type="halBibtex">zaccaria:tel-00820156</idno>
            <idno type="halRefHtml">Médecine humaine et pathologie. Université de Grenoble, 2013. Français. <NNT : 2013GRENS001></idno>
            <idno type="halRef">Médecine humaine et pathologie. Université de Grenoble, 2013. Français. <NNT : 2013GRENS001></idno>
          </publicationStmt>
          <seriesStmt>            <idno type="stamp" n="INSERM">INSERM - Institut national de la santé et de la recherche médicale</idno>
            <idno type="stamp" n="STAR">STAR - Dépôt national des thèses électroniques</idno>
            <idno type="stamp" n="UNIV-GRENOBLE1" p="UGA">Université Joseph Fourier - Grenoble I</idno>
            <idno type="stamp" n="CEA">CEA - Commissariat à l'énergie atomique</idno>
            <idno type="stamp" n="UGA">HAL Grenoble Alpes</idno>
            <idno type="stamp" n="U836" p="UGA">Grenoble Institut des Neurosciences</idno>
          </seriesStmt>
          <notesStmt></notesStmt>
          <sourceDesc>            <biblStruct>              <analytic>                <title xml:lang="en">Strategies for proteomic analysis of cerebral tissue and biological fluids in Parkinson's disease</title>
                <title xml:lang="fr">Nouvelles stratégies pour l'analyse protéomique du tissu cérébral et des fluides biologiques dans la maladie de Parkinson</title>
                <author role="aut">                  <persName>                    <forename type="first">Affif</forename>
                    <forename type="middle">Zaccaria</forename>
                    <surname>Zaccaria</surname>
                  </persName>
                  <idno type="halauthorid">1175555</idno>
                  <idno type="IdRef">http://www.idref.fr/16908986X</idno>
                  <affiliation ref="#struct-86972"></affiliation>
                  <affiliation ref="#struct-421139"></affiliation>
                </author>
              </analytic>
              <monogr>                <idno type="nnt">2013GRENS001</idno>
                <imprint>                  <date type="dateDefended">2013-03-15</date>
                </imprint>
                <authority type="institution">Université de Grenoble</authority>
                <authority type="school">École doctorale ingénierie pour la santé, la cognition, l'environnement (Grenoble)</authority>
                <authority type="supervisor">François Berger</authority>
                <authority type="supervisor">Brigitte Piallat</authority>
                <authority type="jury">Stephan Chabardes [Président]</authority>
                <authority type="jury">Bernard Monsarrat [Rapporteur]</authority>
                <authority type="jury">Pierre Burkhard [Rapporteur]</authority>
                <authority type="jury">Sylvain Lehmann</authority>
                <authority type="jury">Pascal Mossuz</authority>
              </monogr>
            </biblStruct>
          </sourceDesc>
          <profileDesc>            <langUsage>              <language ident="fr">French</language>
            </langUsage>
            <textClass>              <keywords scheme="author">                <term xml:lang="en">Tissue imprint</term>
                <term xml:lang="en">Nanoparticles</term>
                <term xml:lang="en">Parkinson's disease</term>
                <term xml:lang="en">Proteomic</term>
                <term xml:lang="fr">Nanoparticules</term>
                <term xml:lang="fr">Empreinte tissulaire</term>
                <term xml:lang="fr">Proteomique</term>
                <term xml:lang="fr">Maladie de Parkinson</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv.mhep">Life Sciences [q-bio]/Human health and pathology</classCode>
              <classCode scheme="halTypology" n="THESE">Theses</classCode>
            </textClass>
            <abstract xml:lang="en">Proteomics analysis of pathological brain tissue in Parkinson's disease (PD) is of great importance to understand the molecular aetiology of degeneration and to develop curative treatments. To date, published studies have been restricted to the analysis of human post-mortem tissue samples, frequently derived from advanced disease stage and potentially altered by several factors. In this project we took advantage of the temporary access to PD patient's brain during electrode implantation to obtain in vivo molecular information from cerebral tissue at earlier stage of the disease. We further developed a dedicated tool to improve our tissue harvesting approach, and validated its efficiency and non-lesion effects in vivo in monkeys. This work opens the way to the proteomic analysis of fresh human brain samples to elucidate molecular causes of degeneration in PD. However such tissue investigation approach remains invasive and cannot be used in routine clinical screening for PD diagnosis. Proteomics analysis of cerebrospinal fluid (CSF) constitutes a promising alternative to identify neuropathological diagnosis markers. For this purpose, we developed a nanoparticle-assisted strategy enabling the enrichment of CSF proteins detection by mass spectrometry. Reproducibility and high throughput potentiality of our approach demonstrate its compatibility with clinical proteomics for PD diagnosis biomarker research in CSF. We also demonstrated the interest of this NP strategy for plasma and red blood cells proteome analysis. Finally, we evaluated the ability to use these NPs for in vivo protein harvesting in blood.</abstract>
            <abstract xml:lang="fr">L'analyse protéomique du tissu cérébral pathologique dans la maladie de Parkinson (MP) est un enjeu majeur à l'identification des causes moléculaires de la dégénérescence en vue de développer des thérapies curatives. Jusqu'à présent, les études chez l'humain se sont limitées à l'analyse du cerveau post-mortem, trop souvent représentatif d'un stade très avancé de la maladie et potentiellement altéré par différents facteurs. Dans ce travail, nous avons exploité l'accès temporaire au cerveau parkinsonien durant l'implantation d'électrodes de stimulation, pour obtenir une information moléculaire du tissu cérébral, in vivo et à un stade moins avancé de la maladie. Afin d'optimiser notre stratégie, nous avons ensuite développé un outil dédié à la capture tissulaire dont l'efficacité et le caractère non lésionnel ont été validés in vivo chez le primate. Ce travail permet d'envisager l'analyse protéomique du cerveau parkinsonien « vivant » afin d'identifier les causes moléculaires de la MP. En revanche, cette approche tissulaire n'est pas envisageable pour un diagnostic en routine clinique. Aussi, de nombreux groupes s'intéressent à l'analyse protéomique du LCR en vue d'identifier des marqueurs diagnostiques. Dans cette optique, nous avons mis au point une stratégie, basée sur l'utilisation de nanoparticules (NPs) fonctionnalisées qui a permis un enrichissement considérable des profils protéiques observés en spectrométrie de masse. La reproductibilité et la possibilité d'automatiser intégralement la préparation des échantillons font de notre approche une solution adaptée à la recherche de marqueurs moléculaires diagnostiques de la MP dans le LCR. Nous avons aussi démontré l'intérêt de notre approche pour l'analyse protéomique du plasma et du globule rouge. Enfin, nous avons évalué la possibilité d'utiliser ces NPs in vivo, pour une capture des protéines directement dans la circulation sanguine.</abstract>
          </profileDesc>
        </hal>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonFranceV1/Data/Hal/Checkpoint

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000374 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Hal/Checkpoint/biblio.hfd -nk 000374 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Sante
   |area=    ParkinsonFranceV1
   |flux=    Hal
   |étape=   Checkpoint
   |type=    RBID
   |clé=     Hal:tel-00820156
   |texte=   Strategies for proteomic analysis of cerebral tissue and biological fluids in Parkinson's disease
}}

This area was generated with Dilib version V0.6.29.
Data generation: Wed May 17 19:46:39 2017. Site generation: Mon Mar 4 15:48:15 2024

	La maladie de Parkinson en France (serveur d'exploration)
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

La maladie de Parkinson en France (serveur d'exploration)

Strategies for proteomic analysis of cerebral tissue and biological fluids in Parkinson's disease

Strategies for proteomic analysis of cerebral tissue and biological fluids in Parkinson's disease

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri