Enhanced Th1/Th2 mixed immune responses elicited by polyethyleneimine adjuvanted influenza A (H7N9) antigen HA1-2 in chickens.
Identifieur interne : 000266 ( PubMed/Curation ); précédent : 000265; suivant : 000267Enhanced Th1/Th2 mixed immune responses elicited by polyethyleneimine adjuvanted influenza A (H7N9) antigen HA1-2 in chickens.
Auteurs : Li Song [République populaire de Chine] ; Dan Xiong [République populaire de Chine] ; Maozhi Hu [République populaire de Chine] ; Xinan Jiao [République populaire de Chine] ; Zhiming Pan [République populaire de Chine]Source :
- Poultry science [ 1525-3171 ] ; 2018.
Descripteurs français
- KwdFr :
- Adjuvants immunologiques (pharmacologie), Animaux, Antigènes viraux (sang), Attribution aléatoire, Glycoprotéine hémagglutinine du virus influenza (immunologie), Grippe chez les oiseaux (), Grippe chez les oiseaux (immunologie), Immunisation (médecine vétérinaire), Immunité cellulaire, Immunité humorale, Injections musculaires (médecine vétérinaire), Lymphocytes auxiliaires Th1 (immunologie), Lymphocytes auxiliaires Th2 (immunologie), Maladies de la volaille (), Maladies de la volaille (immunologie), Organismes exempts d'organismes pathogènes spécifiques, Polyéthylèneimine (pharmacologie), Poulets (immunologie), Sous-type H7N9 du virus de la grippe A ().
- MESH :
- immunologie : Glycoprotéine hémagglutinine du virus influenza, Grippe chez les oiseaux, Lymphocytes auxiliaires Th1, Lymphocytes auxiliaires Th2, Maladies de la volaille, Poulets.
- médecine vétérinaire : Immunisation, Injections musculaires.
- pharmacologie : Adjuvants immunologiques, Polyéthylèneimine.
- sang : Antigènes viraux.
- Animaux, Attribution aléatoire, Grippe chez les oiseaux, Immunité cellulaire, Immunité humorale, Maladies de la volaille, Organismes exempts d'organismes pathogènes spécifiques, Sous-type H7N9 du virus de la grippe A.
English descriptors
- KwdEn :
- Adjuvants, Immunologic (pharmacology), Animals, Antigens, Viral (blood), Chickens (immunology), Hemagglutinin Glycoproteins, Influenza Virus (immunology), Immunity, Cellular, Immunity, Humoral, Immunization (veterinary), Influenza A Virus, H7N9 Subtype (drug effects), Influenza in Birds (immunology), Influenza in Birds (prevention & control), Injections, Intramuscular (veterinary), Polyethyleneimine (pharmacology), Poultry Diseases (immunology), Poultry Diseases (prevention & control), Random Allocation, Specific Pathogen-Free Organisms, Th1 Cells (immunology), Th2 Cells (immunology).
- MESH :
- chemical , blood : Antigens, Viral.
- chemical , immunology : Hemagglutinin Glycoproteins, Influenza Virus.
- chemical , pharmacology : Adjuvants, Immunologic, Polyethyleneimine.
- drug effects : Influenza A Virus, H7N9 Subtype.
- immunology : Chickens, Influenza in Birds, Poultry Diseases, Th1 Cells, Th2 Cells.
- prevention & control : Influenza in Birds, Poultry Diseases.
- veterinary : Immunization, Injections, Intramuscular.
- Animals, Immunity, Cellular, Immunity, Humoral, Random Allocation, Specific Pathogen-Free Organisms.
Abstract
Influenza A (H7N9) viruses have caused severe human infections and deaths every year in China since 2013. To reduce the risk of human infection and prevent a new influenza pandemic, there is a pressing need to develop safe and effective anti-H7N9 vaccines for poultry. Polyethyleneimine (PEI) is an organic polycation used extensively as a transfection reagent for decades. Although the adjuvant potential of PEI is well studied in mammals, its applicability and immune characteristics to avian species are still very rare. Here, to investigate the adjuvant activity of PEI, we analyzed the immune responses in chicken peripheral blood mononuclear cells in vitro. PEI significantly upregulated the expression of immune-related cytokines (IFN-γ, IL-2, IL-4, IL-6, IL-18, and IL-1β) and chemokines (CXCLi1, CXCLi2, MIP-1β, and MCP-3), suggesting that PEI promoted immune responses of avian cells. We also assessed the in vivo immune responses to PEI in a chicken model. After the second and third vaccinations, significantly higher IgG titers were observed in the chickens immunized with HA1-2+PEI than that of HA1-2 alone. The HA1-2+PEI group also increased percentages of CD4+ and CD8+ T cells and improved PBMC proliferation. The significantly upregulated IFN-γ and IL-4 levels of splenocytes from HA1-2+PEI vaccinated chickens further indicated that PEI promoted a Th1/Th2 mixed immune responses. This study not only demonstrates the adjuvant potential of PEI when co-administered with influenza H7N9 antigen HA1-2 in chickens, but also supports the use of PEI as a versatile systemic adjuvant platform in poultry.
DOI: 10.3382/ps/pey313
PubMed: 30085299
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Cellular</term><term>Immunity, Humoral</term><term>Random Allocation</term><term>Specific Pathogen-Free Organisms</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Attribution aléatoire</term><term>Grippe chez les oiseaux</term><term>Immunité cellulaire</term><term>Immunité humorale</term><term>Maladies de la volaille</term><term>Organismes exempts d'organismes pathogènes spécifiques</term><term>Sous-type H7N9 du virus de la grippe A</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Influenza A (H7N9) viruses have caused severe human infections and deaths every year in China since 2013. To reduce the risk of human infection and prevent a new influenza pandemic, there is a pressing need to develop safe and effective anti-H7N9 vaccines for poultry. Polyethyleneimine (PEI) is an organic polycation used extensively as a transfection reagent for decades. Although the adjuvant potential of PEI is well studied in mammals, its applicability and immune characteristics to avian species are still very rare. Here, to investigate the adjuvant activity of PEI, we analyzed the immune responses in chicken peripheral blood mononuclear cells in vitro. PEI significantly upregulated the expression of immune-related cytokines (IFN-γ, IL-2, IL-4, IL-6, IL-18, and IL-1β) and chemokines (CXCLi1, CXCLi2, MIP-1β, and MCP-3), suggesting that PEI promoted immune responses of avian cells. We also assessed the in vivo immune responses to PEI in a chicken model. After the second and third vaccinations, significantly higher IgG titers were observed in the chickens immunized with HA1-2+PEI than that of HA1-2 alone. The HA1-2+PEI group also increased percentages of CD4+ and CD8+ T cells and improved PBMC proliferation. The significantly upregulated IFN-γ and IL-4 levels of splenocytes from HA1-2+PEI vaccinated chickens further indicated that PEI promoted a Th1/Th2 mixed immune responses. This study not only demonstrates the adjuvant potential of PEI when co-administered with influenza H7N9 antigen HA1-2 in chickens, but also supports the use of PEI as a versatile systemic adjuvant platform in poultry.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30085299</PMID><DateCompleted><Year>2019</Year><Month>01</Month><Day>11</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1525-3171</ISSN><JournalIssue CitedMedium="Internet"><Volume>97</Volume><Issue>12</Issue><PubDate><Year>2018</Year><Month>Dec</Month><Day>01</Day></PubDate></JournalIssue><Title>Poultry science</Title><ISOAbbreviation>Poult. Sci.</ISOAbbreviation></Journal><ArticleTitle>Enhanced Th1/Th2 mixed immune responses elicited by polyethyleneimine adjuvanted influenza A (H7N9) antigen HA1-2 in chickens.</ArticleTitle><Pagination><MedlinePgn>4245-4251</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3382/ps/pey313</ELocationID><Abstract><AbstractText>Influenza A (H7N9) viruses have caused severe human infections and deaths every year in China since 2013. To reduce the risk of human infection and prevent a new influenza pandemic, there is a pressing need to develop safe and effective anti-H7N9 vaccines for poultry. Polyethyleneimine (PEI) is an organic polycation used extensively as a transfection reagent for decades. Although the adjuvant potential of PEI is well studied in mammals, its applicability and immune characteristics to avian species are still very rare. Here, to investigate the adjuvant activity of PEI, we analyzed the immune responses in chicken peripheral blood mononuclear cells in vitro. PEI significantly upregulated the expression of immune-related cytokines (IFN-γ, IL-2, IL-4, IL-6, IL-18, and IL-1β) and chemokines (CXCLi1, CXCLi2, MIP-1β, and MCP-3), suggesting that PEI promoted immune responses of avian cells. We also assessed the in vivo immune responses to PEI in a chicken model. After the second and third vaccinations, significantly higher IgG titers were observed in the chickens immunized with HA1-2+PEI than that of HA1-2 alone. The HA1-2+PEI group also increased percentages of CD4+ and CD8+ T cells and improved PBMC proliferation. The significantly upregulated IFN-γ and IL-4 levels of splenocytes from HA1-2+PEI vaccinated chickens further indicated that PEI promoted a Th1/Th2 mixed immune responses. This study not only demonstrates the adjuvant potential of PEI when co-administered with influenza H7N9 antigen HA1-2 in chickens, but also supports the use of PEI as a versatile systemic adjuvant platform in poultry.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Li</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiong</LastName><ForeName>Dan</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Maozhi</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiao</LastName><ForeName>Xinan</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pan</LastName><ForeName>Zhiming</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou, Jiangsu 225009, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Poult Sci</MedlineTA><NlmUniqueID>0401150</NlmUniqueID><ISSNLinking>0032-5791</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000276">Adjuvants, Immunologic</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000956">Antigens, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019267">Hemagglutinin Glycoproteins, Influenza 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MajorTopicYN="N">Th2 Cells</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>12</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>07</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>8</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>1</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>8</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30085299</ArticleId><ArticleId IdType="pii">S0032-5791(19)30253-6</ArticleId><ArticleId IdType="doi">10.3382/ps/pey313</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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