Characterization of the reconstructed 1918 Spanish influenza pandemic virus.
Identifieur interne : 001E29 ( PubMed/Corpus ); précédent : 001E28; suivant : 001E30Characterization of the reconstructed 1918 Spanish influenza pandemic virus.
Auteurs : Terrence M. Tumpey ; Christopher F. Basler ; Patricia V. Aguilar ; Hui Zeng ; Alicia Sol Rzano ; David E. Swayne ; Nancy J. Cox ; Jacqueline M. Katz ; Jeffery K. Taubenberger ; Peter Palese ; Adolfo García-SastreSource :
- Science (New York, N.Y.) [ 1095-9203 ] ; 2005.
English descriptors
- KwdEn :
- Animals, Bronchi (virology), Cell Line, Chick Embryo (virology), Female, Genes, Viral, Genetic Techniques, Hemagglutinin Glycoproteins, Influenza Virus (genetics), Hemagglutinin Glycoproteins, Influenza Virus (metabolism), History, 20th Century, Humans, Influenza A Virus, H1N1 Subtype (genetics), Influenza A Virus, H1N1 Subtype (pathogenicity), Influenza A Virus, H1N1 Subtype (physiology), Influenza, Human (epidemiology), Influenza, Human (history), Influenza, Human (virology), Lung (pathology), Lung (virology), Mice, Mice, Inbred BALB C, Neuraminidase (genetics), Neuraminidase (metabolism), Orthomyxoviridae Infections (pathology), Orthomyxoviridae Infections (virology), RNA, Viral (genetics), Recombination, Genetic, Respiratory Mucosa (virology), Trypsin (metabolism), Viral Plaque Assay, Virulence (genetics), Virus Replication.
- MESH :
- chemical , genetics : Hemagglutinin Glycoproteins, Influenza Virus, Neuraminidase, RNA, Viral.
- chemical , metabolism : Hemagglutinin Glycoproteins, Influenza Virus, Neuraminidase, Trypsin.
- epidemiology : Influenza, Human.
- genetics : Influenza A Virus, H1N1 Subtype, Virulence.
- history : Influenza, Human.
- pathogenicity : Influenza A Virus, H1N1 Subtype.
- pathology : Lung, Orthomyxoviridae Infections.
- physiology : Influenza A Virus, H1N1 Subtype.
- virology : Bronchi, Chick Embryo, Influenza, Human, Lung, Orthomyxoviridae Infections, Respiratory Mucosa.
- Animals, Cell Line, Female, Genes, Viral, Genetic Techniques, History, 20th Century, Humans, Mice, Mice, Inbred BALB C, Recombination, Genetic, Viral Plaque Assay, Virus Replication.
Abstract
The pandemic influenza virus of 1918-1919 killed an estimated 20 to 50 million people worldwide. With the recent availability of the complete 1918 influenza virus coding sequence, we used reverse genetics to generate an influenza virus bearing all eight gene segments of the pandemic virus to study the properties associated with its extraordinary virulence. In stark contrast to contemporary human influenza H1N1 viruses, the 1918 pandemic virus had the ability to replicate in the absence of trypsin, caused death in mice and embryonated chicken eggs, and displayed a high-growth phenotype in human bronchial epithelial cells. Moreover, the coordinated expression of the 1918 virus genes most certainly confers the unique high-virulence phenotype observed with this pandemic virus.
DOI: 10.1126/science.1119392
PubMed: 16210530
Links to Exploration step
pubmed:16210530Le document en format XML
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Aguilar</name></author><author><name sortKey="Zeng, Hui" sort="Zeng, Hui" uniqKey="Zeng H" first="Hui" last="Zeng">Hui Zeng</name></author><author><name sortKey="Sol Rzano, Alicia" sort="Sol Rzano, Alicia" uniqKey="Sol Rzano A" first="Alicia" last="Sol Rzano">Alicia Sol Rzano</name></author><author><name sortKey="Swayne, David E" sort="Swayne, David E" uniqKey="Swayne D" first="David E" last="Swayne">David E. Swayne</name></author><author><name sortKey="Cox, Nancy J" sort="Cox, Nancy J" uniqKey="Cox N" first="Nancy J" last="Cox">Nancy J. Cox</name></author><author><name sortKey="Katz, Jacqueline M" sort="Katz, Jacqueline M" uniqKey="Katz J" first="Jacqueline M" last="Katz">Jacqueline M. Katz</name></author><author><name sortKey="Taubenberger, Jeffery K" sort="Taubenberger, Jeffery K" uniqKey="Taubenberger J" first="Jeffery K" last="Taubenberger">Jeffery K. 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Tumpey</name><affiliation><nlm:affiliation>Influenza Branch, Mailstop G-16, Division of Viral and Rickettsial Diseases (DVRD), National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, NE, Atlanta, GA 30333, USA. tft9@cdc.gov</nlm:affiliation></affiliation></author><author><name sortKey="Basler, Christopher F" sort="Basler, Christopher F" uniqKey="Basler C" first="Christopher F" last="Basler">Christopher F. Basler</name></author><author><name sortKey="Aguilar, Patricia V" sort="Aguilar, Patricia V" uniqKey="Aguilar P" first="Patricia V" last="Aguilar">Patricia V. 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With the recent availability of the complete 1918 influenza virus coding sequence, we used reverse genetics to generate an influenza virus bearing all eight gene segments of the pandemic virus to study the properties associated with its extraordinary virulence. In stark contrast to contemporary human influenza H1N1 viruses, the 1918 pandemic virus had the ability to replicate in the absence of trypsin, caused death in mice and embryonated chicken eggs, and displayed a high-growth phenotype in human bronchial epithelial cells. Moreover, the coordinated expression of the 1918 virus genes most certainly confers the unique high-virulence phenotype observed with this pandemic virus.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16210530</PMID><DateCompleted><Year>2005</Year><Month>11</Month><Day>03</Day></DateCompleted><DateRevised><Year>2011</Year><Month>11</Month><Day>17</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1095-9203</ISSN><JournalIssue CitedMedium="Internet"><Volume>310</Volume><Issue>5745</Issue><PubDate><Year>2005</Year><Month>Oct</Month><Day>07</Day></PubDate></JournalIssue><Title>Science (New York, N.Y.)</Title><ISOAbbreviation>Science</ISOAbbreviation></Journal><ArticleTitle>Characterization of the reconstructed 1918 Spanish influenza pandemic virus.</ArticleTitle><Pagination><MedlinePgn>77-80</MedlinePgn></Pagination><Abstract><AbstractText>The pandemic influenza virus of 1918-1919 killed an estimated 20 to 50 million people worldwide. With the recent availability of the complete 1918 influenza virus coding sequence, we used reverse genetics to generate an influenza virus bearing all eight gene segments of the pandemic virus to study the properties associated with its extraordinary virulence. In stark contrast to contemporary human influenza H1N1 viruses, the 1918 pandemic virus had the ability to replicate in the absence of trypsin, caused death in mice and embryonated chicken eggs, and displayed a high-growth phenotype in human bronchial epithelial cells. Moreover, the coordinated expression of the 1918 virus genes most certainly confers the unique high-virulence phenotype observed with this pandemic virus.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tumpey</LastName><ForeName>Terrence M</ForeName><Initials>TM</Initials><AffiliationInfo><Affiliation>Influenza Branch, Mailstop G-16, Division of Viral and Rickettsial Diseases (DVRD), National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, NE, Atlanta, GA 30333, USA. tft9@cdc.gov</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Basler</LastName><ForeName>Christopher F</ForeName><Initials>CF</Initials></Author><Author ValidYN="Y"><LastName>Aguilar</LastName><ForeName>Patricia V</ForeName><Initials>PV</Initials></Author><Author ValidYN="Y"><LastName>Zeng</LastName><ForeName>Hui</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Solórzano</LastName><ForeName>Alicia</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Swayne</LastName><ForeName>David E</ForeName><Initials>DE</Initials></Author><Author ValidYN="Y"><LastName>Cox</LastName><ForeName>Nancy J</ForeName><Initials>NJ</Initials></Author><Author ValidYN="Y"><LastName>Katz</LastName><ForeName>Jacqueline M</ForeName><Initials>JM</Initials></Author><Author ValidYN="Y"><LastName>Taubenberger</LastName><ForeName>Jeffery K</ForeName><Initials>JK</Initials></Author><Author ValidYN="Y"><LastName>Palese</LastName><ForeName>Peter</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>García-Sastre</LastName><ForeName>Adolfo</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>P01 AI058113-01</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>U19 AI62623</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>U54 AI57158</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016456">Historical Article</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Science</MedlineTA><NlmUniqueID>0404511</NlmUniqueID><ISSNLinking>0036-8075</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019267">Hemagglutinin Glycoproteins, Influenza Virus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.18</RegistryNumber><NameOfSubstance UI="D009439">Neuraminidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.21.4</RegistryNumber><NameOfSubstance UI="D014357">Trypsin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="CommentIn"><RefSource>Science. 2005 Oct 7;310(5745):17</RefSource><PMID Version="1">16210500</PMID></CommentsCorrections><CommentsCorrections RefType="CommentIn"><RefSource>Science. 2005 Oct 7;310(5745):28-9</RefSource><PMID Version="1">16210501</PMID></CommentsCorrections><CommentsCorrections RefType="CommentIn"><RefSource>Science. 2005 Oct 14;310(5746):195</RefSource><PMID Version="1">16223986</PMID></CommentsCorrections><CommentsCorrections RefType="CommentIn"><RefSource>Science. 2006 Jan 20;311(5759):336-7</RefSource><PMID Version="1">16424324</PMID></CommentsCorrections><CommentsCorrections RefType="CommentIn"><RefSource>Science. 2006 Mar 17;311(5767):1552; author reply 1552</RefSource><PMID Version="1">16543439</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001980" MajorTopicYN="N">Bronchi</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002642" MajorTopicYN="N">Chick Embryo</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005814" MajorTopicYN="Y">Genes, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005821" MajorTopicYN="N">Genetic Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019267" MajorTopicYN="N">Hemagglutinin Glycoproteins, Influenza Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049673" MajorTopicYN="N">History, 20th Century</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053118" MajorTopicYN="N">Influenza A Virus, H1N1 Subtype</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName><QualifierName UI="Q000266" MajorTopicYN="N">history</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008168" MajorTopicYN="N">Lung</DescriptorName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009439" MajorTopicYN="N">Neuraminidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009976" MajorTopicYN="N">Orthomyxoviridae Infections</DescriptorName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011995" MajorTopicYN="N">Recombination, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020545" MajorTopicYN="N">Respiratory Mucosa</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014357" MajorTopicYN="N">Trypsin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010948" MajorTopicYN="N">Viral Plaque Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014779" MajorTopicYN="N">Virus Replication</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>10</Month><Day>8</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>11</Month><Day>4</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>10</Month><Day>8</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16210530</ArticleId><ArticleId IdType="pii">310/5745/77</ArticleId><ArticleId IdType="doi">10.1126/science.1119392</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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