PandemieGrippaleV1, PubMed, Checkpoint, bibRecord, 000525

Conserved stem fragment from H3 influenza hemagglutinin elicits cross-clade neutralizing antibodies through stalk-targeted blocking of conformational change during membrane fusion.

Identifieur interne : 000525 ( PubMed/Checkpoint ); précédent : 000524; suivant : 000526

Conserved stem fragment from H3 influenza hemagglutinin elicits cross-clade neutralizing antibodies through stalk-targeted blocking of conformational change during membrane fusion.

Source :

Immunology letters [ 1879-0542 ] ; 2016.

RBID : pubmed:26875772

Descripteurs français

English descriptors

KwdEn :
- Animals, Antibodies, Neutralizing (metabolism), Antibodies, Viral (metabolism), Combinatorial Chemistry Techniques, Computer Simulation, Conserved Sequence (genetics), Crystallography, X-Ray, Epitope Mapping, Epitopes, B-Lymphocyte (chemistry), Epitopes, B-Lymphocyte (genetics), Epitopes, B-Lymphocyte (immunology), Epitopes, T-Lymphocyte (chemistry), Epitopes, T-Lymphocyte (genetics), Epitopes, T-Lymphocyte (immunology), Female, Hemagglutinin Glycoproteins, Influenza Virus (chemistry), Hemagglutinin Glycoproteins, Influenza Virus (immunology), Humans, Influenza A Virus, H3N2 Subtype (immunology), Influenza Vaccines (immunology), Influenza, Human (immunology), Membrane Fusion, Mice, Mice, Inbred BALB C, Orthomyxoviridae Infections (immunology), Protein Conformation, Sequence Alignment.
MESH :
- chemical , chemistry : Epitopes, B-Lymphocyte, Epitopes, T-Lymphocyte, Hemagglutinin Glycoproteins, Influenza Virus.
- chemical , genetics : Epitopes, B-Lymphocyte, Epitopes, T-Lymphocyte.
- chemical , immunology : Epitopes, B-Lymphocyte, Epitopes, T-Lymphocyte, Hemagglutinin Glycoproteins, Influenza Virus, Influenza Vaccines.
- chemical , metabolism : Antibodies, Neutralizing, Antibodies, Viral.
- genetics : Conserved Sequence.
- immunology : Influenza A Virus, H3N2 Subtype, Influenza, Human, Orthomyxoviridae Infections.
- Animals, Combinatorial Chemistry Techniques, Computer Simulation, Crystallography, X-Ray, Epitope Mapping, Female, Humans, Membrane Fusion, Mice, Mice, Inbred BALB C, Protein Conformation, Sequence Alignment.

Abstract

Currently available influenza vaccines typically fail to elicit/boost broadly neutralizing antibodies due to the mutability of virus sequences and conformational changes during protective immunity, thereby limiting their efficacy. This problem needs to be addressed by further understanding the mechanisms of neutralization and finding the desired neutralizing site during membrane fusion. This study specifically focused on viruses of the H3N2 subtype, which have persisted as a principal source of influenza-related morbidity and mortality in humans since the 1968 influenza pandemic. Through sequence alignment and epitope prediction, a series of highly conserved stem fragments (spanning 47 years) were found and coupled to the Keyhole Limpet Hemocyanin (KLH) protein. By application of a combinatorial display library and crystal structure modeling, a stem fragment immunogen, located at the turning point of the HA neck undergoing conformational change during membrane fusion with both B- and T-cell epitopes, was identified. After synthesis of the optimal stem fragment using a multiple antigen peptide (MAP) system, strong humoral immune responses and cross-clade neutralizing activities against strains from the H3 subtype of group 2 influenza viruses after animal immunizations were observed. By detection of nuclear protein immunofluorescence with acid bypass treatment, antisera raised against MAP4 immunogens of the stem fragment showed the potential to inhibit the conformational change of HA in stem-targeted virus neutralization. The identification of this conserved stem fragment provides great potential for exploitation of this site of vulnerability in therapeutic and vaccine design.

DOI: 10.1016/j.imlet.2016.02.006
PubMed: 26875772

Affiliations:

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Le document en format XML

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<author><name sortKey="Yang, Lan" sort="Yang, Lan" uniqKey="Yang L" first="Lan" last="Yang">Lan Yang</name>
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<author><name sortKey="Yu, Yongjiao" sort="Yu, Yongjiao" uniqKey="Yu Y" first="Yongjiao" last="Yu">Yongjiao Yu</name>
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<author><name sortKey="Kuai, Ziyu" sort="Kuai, Ziyu" uniqKey="Kuai Z" first="Ziyu" last="Kuai">Ziyu Kuai</name>
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<author><name sortKey="Wang, Song" sort="Wang, Song" uniqKey="Wang S" first="Song" last="Wang">Song Wang</name>
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<author><name sortKey="Shan, Yaming" sort="Shan, Yaming" uniqKey="Shan Y" first="Yaming" last="Shan">Yaming Shan</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Conserved stem fragment from H3 influenza hemagglutinin elicits cross-clade neutralizing antibodies through stalk-targeted blocking of conformational change during membrane fusion.</title>
<author><name sortKey="Gong, Xin" sort="Gong, Xin" uniqKey="Gong X" first="Xin" last="Gong">Xin Gong</name>
<affiliation wicri:level="4"><nlm:affiliation>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin</wicri:regionArea>
<orgName type="university">Université de Jilin</orgName>
<placeName><settlement type="city">Changchun</settlement>
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<author><name sortKey="Yin, He" sort="Yin, He" uniqKey="Yin H" first="He" last="Yin">He Yin</name>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin</wicri:regionArea>
<orgName type="university">Université de Jilin</orgName>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
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</author>
<author><name sortKey="Shi, Yuhua" sort="Shi, Yuhua" uniqKey="Shi Y" first="Yuhua" last="Shi">Yuhua Shi</name>
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<country xml:lang="fr">République populaire de Chine</country>
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<orgName type="university">Université de Jilin</orgName>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
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<author><name sortKey="Guan, Shanshan" sort="Guan, Shanshan" uniqKey="Guan S" first="Shanshan" last="Guan">Shanshan Guan</name>
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<country xml:lang="fr">République populaire de Chine</country>
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<orgName type="university">Université de Jilin</orgName>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="He, Xiaoqiu" sort="He, Xiaoqiu" uniqKey="He X" first="Xiaoqiu" last="He">Xiaoqiu He</name>
<affiliation wicri:level="4"><nlm:affiliation>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin</wicri:regionArea>
<orgName type="university">Université de Jilin</orgName>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
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<author><name sortKey="Yang, Lan" sort="Yang, Lan" uniqKey="Yang L" first="Lan" last="Yang">Lan Yang</name>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin</wicri:regionArea>
<orgName type="university">Université de Jilin</orgName>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yu, Yongjiao" sort="Yu, Yongjiao" uniqKey="Yu Y" first="Yongjiao" last="Yu">Yongjiao Yu</name>
<affiliation wicri:level="4"><nlm:affiliation>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin</wicri:regionArea>
<orgName type="university">Université de Jilin</orgName>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kuai, Ziyu" sort="Kuai, Ziyu" uniqKey="Kuai Z" first="Ziyu" last="Kuai">Ziyu Kuai</name>
<affiliation wicri:level="4"><nlm:affiliation>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin</wicri:regionArea>
<orgName type="university">Université de Jilin</orgName>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Jiang, Chunlai" sort="Jiang, Chunlai" uniqKey="Jiang C" first="Chunlai" last="Jiang">Chunlai Jiang</name>
<affiliation wicri:level="4"><nlm:affiliation>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China; Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun, Jilin, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China; Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun, Jilin</wicri:regionArea>
<orgName type="university">Université de Jilin</orgName>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kong, Wei" sort="Kong, Wei" uniqKey="Kong W" first="Wei" last="Kong">Wei Kong</name>
<affiliation wicri:level="4"><nlm:affiliation>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China; Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun, Jilin, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China; Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun, Jilin</wicri:regionArea>
<orgName type="university">Université de Jilin</orgName>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wang, Song" sort="Wang, Song" uniqKey="Wang S" first="Song" last="Wang">Song Wang</name>
<affiliation wicri:level="4"><nlm:affiliation>State Key Laboratory of Theoretical and Computational Chemistry, Institute of Theoretical Chemistry, Jilin University, Changchun, Jilin, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Theoretical and Computational Chemistry, Institute of Theoretical Chemistry, Jilin University, Changchun, Jilin</wicri:regionArea>
<orgName type="university">Université de Jilin</orgName>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Shan, Yaming" sort="Shan, Yaming" uniqKey="Shan Y" first="Yaming" last="Shan">Yaming Shan</name>
<affiliation wicri:level="4"><nlm:affiliation>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China; Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun, Jilin, China. Electronic address: shanym@jlu.edu.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China; Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun, Jilin</wicri:regionArea>
<orgName type="university">Université de Jilin</orgName>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Immunology letters</title>
<idno type="eISSN">1879-0542</idno>
<imprint><date when="2016" type="published">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antibodies, Neutralizing (metabolism)</term>
<term>Antibodies, Viral (metabolism)</term>
<term>Combinatorial Chemistry Techniques</term>
<term>Computer Simulation</term>
<term>Conserved Sequence (genetics)</term>
<term>Crystallography, X-Ray</term>
<term>Epitope Mapping</term>
<term>Epitopes, B-Lymphocyte (chemistry)</term>
<term>Epitopes, B-Lymphocyte (genetics)</term>
<term>Epitopes, B-Lymphocyte (immunology)</term>
<term>Epitopes, T-Lymphocyte (chemistry)</term>
<term>Epitopes, T-Lymphocyte (genetics)</term>
<term>Epitopes, T-Lymphocyte (immunology)</term>
<term>Female</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (chemistry)</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (immunology)</term>
<term>Humans</term>
<term>Influenza A Virus, H3N2 Subtype (immunology)</term>
<term>Influenza Vaccines (immunology)</term>
<term>Influenza, Human (immunology)</term>
<term>Membrane Fusion</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Orthomyxoviridae Infections (immunology)</term>
<term>Protein Conformation</term>
<term>Sequence Alignment</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences</term>
<term>Animaux</term>
<term>Anticorps antiviraux (métabolisme)</term>
<term>Anticorps neutralisants (métabolisme)</term>
<term>Cartographie épitopique</term>
<term>Conformation des protéines</term>
<term>Cristallographie aux rayons X</term>
<term>Déterminants antigéniques des lymphocytes B ()</term>
<term>Déterminants antigéniques des lymphocytes B (génétique)</term>
<term>Déterminants antigéniques des lymphocytes B (immunologie)</term>
<term>Déterminants antigéniques des lymphocytes T ()</term>
<term>Déterminants antigéniques des lymphocytes T (génétique)</term>
<term>Déterminants antigéniques des lymphocytes T (immunologie)</term>
<term>Femelle</term>
<term>Fusion membranaire</term>
<term>Glycoprotéine hémagglutinine du virus influenza ()</term>
<term>Glycoprotéine hémagglutinine du virus influenza (immunologie)</term>
<term>Grippe humaine (immunologie)</term>
<term>Humains</term>
<term>Infections à Orthomyxoviridae (immunologie)</term>
<term>Simulation numérique</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Sous-type H3N2 du virus de la grippe A (immunologie)</term>
<term>Séquence conservée (génétique)</term>
<term>Techniques de chimie combinatoire</term>
<term>Vaccins antigrippaux (immunologie)</term>
</keywords>
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<term>Epitopes, T-Lymphocyte</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Epitopes, B-Lymphocyte</term>
<term>Epitopes, T-Lymphocyte</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Epitopes, B-Lymphocyte</term>
<term>Epitopes, T-Lymphocyte</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus</term>
<term>Influenza Vaccines</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antibodies, Neutralizing</term>
<term>Antibodies, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Conserved Sequence</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Déterminants antigéniques des lymphocytes B</term>
<term>Déterminants antigéniques des lymphocytes T</term>
<term>Séquence conservée</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Déterminants antigéniques des lymphocytes B</term>
<term>Déterminants antigéniques des lymphocytes T</term>
<term>Glycoprotéine hémagglutinine du virus influenza</term>
<term>Grippe humaine</term>
<term>Infections à Orthomyxoviridae</term>
<term>Sous-type H3N2 du virus de la grippe A</term>
<term>Vaccins antigrippaux</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A Virus, H3N2 Subtype</term>
<term>Influenza, Human</term>
<term>Orthomyxoviridae Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Anticorps neutralisants</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Combinatorial Chemistry Techniques</term>
<term>Computer Simulation</term>
<term>Crystallography, X-Ray</term>
<term>Epitope Mapping</term>
<term>Female</term>
<term>Humans</term>
<term>Membrane Fusion</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Protein Conformation</term>
<term>Sequence Alignment</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term>
<term>Animaux</term>
<term>Cartographie épitopique</term>
<term>Conformation des protéines</term>
<term>Cristallographie aux rayons X</term>
<term>Déterminants antigéniques des lymphocytes B</term>
<term>Déterminants antigéniques des lymphocytes T</term>
<term>Femelle</term>
<term>Fusion membranaire</term>
<term>Glycoprotéine hémagglutinine du virus influenza</term>
<term>Humains</term>
<term>Simulation numérique</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Techniques de chimie combinatoire</term>
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<front><div type="abstract" xml:lang="en">Currently available influenza vaccines typically fail to elicit/boost broadly neutralizing antibodies due to the mutability of virus sequences and conformational changes during protective immunity, thereby limiting their efficacy. This problem needs to be addressed by further understanding the mechanisms of neutralization and finding the desired neutralizing site during membrane fusion. This study specifically focused on viruses of the H3N2 subtype, which have persisted as a principal source of influenza-related morbidity and mortality in humans since the 1968 influenza pandemic. Through sequence alignment and epitope prediction, a series of highly conserved stem fragments (spanning 47 years) were found and coupled to the Keyhole Limpet Hemocyanin (KLH) protein. By application of a combinatorial display library and crystal structure modeling, a stem fragment immunogen, located at the turning point of the HA neck undergoing conformational change during membrane fusion with both B- and T-cell epitopes, was identified. After synthesis of the optimal stem fragment using a multiple antigen peptide (MAP) system, strong humoral immune responses and cross-clade neutralizing activities against strains from the H3 subtype of group 2 influenza viruses after animal immunizations were observed. By detection of nuclear protein immunofluorescence with acid bypass treatment, antisera raised against MAP4 immunogens of the stem fragment showed the potential to inhibit the conformational change of HA in stem-targeted virus neutralization. The identification of this conserved stem fragment provides great potential for exploitation of this site of vulnerability in therapeutic and vaccine design.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26875772</PMID>
<DateCompleted><Year>2017</Year>
<Month>11</Month>
<Day>28</Day>
</DateCompleted>
<DateRevised><Year>2017</Year>
<Month>12</Month>
<Day>16</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0542</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>172</Volume>
<PubDate><Year>2016</Year>
<Month>04</Month>
</PubDate>
</JournalIssue>
<Title>Immunology letters</Title>
<ISOAbbreviation>Immunol. Lett.</ISOAbbreviation>
</Journal>
<ArticleTitle>Conserved stem fragment from H3 influenza hemagglutinin elicits cross-clade neutralizing antibodies through stalk-targeted blocking of conformational change during membrane fusion.</ArticleTitle>
<Pagination><MedlinePgn>11-20</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1016/j.imlet.2016.02.006</ELocationID>
<ELocationID EIdType="pii" ValidYN="Y">S0165-2478(16)30015-3</ELocationID>
<Abstract><AbstractText>Currently available influenza vaccines typically fail to elicit/boost broadly neutralizing antibodies due to the mutability of virus sequences and conformational changes during protective immunity, thereby limiting their efficacy. This problem needs to be addressed by further understanding the mechanisms of neutralization and finding the desired neutralizing site during membrane fusion. This study specifically focused on viruses of the H3N2 subtype, which have persisted as a principal source of influenza-related morbidity and mortality in humans since the 1968 influenza pandemic. Through sequence alignment and epitope prediction, a series of highly conserved stem fragments (spanning 47 years) were found and coupled to the Keyhole Limpet Hemocyanin (KLH) protein. By application of a combinatorial display library and crystal structure modeling, a stem fragment immunogen, located at the turning point of the HA neck undergoing conformational change during membrane fusion with both B- and T-cell epitopes, was identified. After synthesis of the optimal stem fragment using a multiple antigen peptide (MAP) system, strong humoral immune responses and cross-clade neutralizing activities against strains from the H3 subtype of group 2 influenza viruses after animal immunizations were observed. By detection of nuclear protein immunofluorescence with acid bypass treatment, antisera raised against MAP4 immunogens of the stem fragment showed the potential to inhibit the conformational change of HA in stem-targeted virus neutralization. The identification of this conserved stem fragment provides great potential for exploitation of this site of vulnerability in therapeutic and vaccine design.</AbstractText>
<CopyrightInformation>Copyright © 2016 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gong</LastName>
<ForeName>Xin</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yin</LastName>
<ForeName>He</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Shi</LastName>
<ForeName>Yuhua</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Guan</LastName>
<ForeName>Shanshan</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>He</LastName>
<ForeName>Xiaoqiu</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yang</LastName>
<ForeName>Lan</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yu</LastName>
<ForeName>Yongjiao</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kuai</LastName>
<ForeName>Ziyu</ForeName>
<Initials>Z</Initials>
<AffiliationInfo><Affiliation>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jiang</LastName>
<ForeName>Chunlai</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China; Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun, Jilin, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kong</LastName>
<ForeName>Wei</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China; Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun, Jilin, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Song</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Theoretical and Computational Chemistry, Institute of Theoretical Chemistry, Jilin University, Changchun, Jilin, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Shan</LastName>
<ForeName>Yaming</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, China; Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun, Jilin, China. Electronic address: shanym@jlu.edu.cn.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>02</Month>
<Day>10</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Netherlands</Country>
<MedlineTA>Immunol Lett</MedlineTA>
<NlmUniqueID>7910006</NlmUniqueID>
<ISSNLinking>0165-2478</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D057134">Antibodies, Neutralizing</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D018985">Epitopes, B-Lymphocyte</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D018984">Epitopes, T-Lymphocyte</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019267">Hemagglutinin Glycoproteins, Influenza Virus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D007252">Influenza Vaccines</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D057134" MajorTopicYN="N">Antibodies, Neutralizing</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020650" MajorTopicYN="N">Combinatorial Chemistry Techniques</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003198" MajorTopicYN="N">Computer Simulation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017124" MajorTopicYN="N">Conserved Sequence</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018360" MajorTopicYN="N">Crystallography, X-Ray</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018604" MajorTopicYN="N">Epitope Mapping</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018985" MajorTopicYN="N">Epitopes, B-Lymphocyte</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018984" MajorTopicYN="N">Epitopes, T-Lymphocyte</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
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<MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019267" MajorTopicYN="N">Hemagglutinin Glycoproteins, Influenza Virus</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D053122" MajorTopicYN="N">Influenza A Virus, H3N2 Subtype</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
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<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
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<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008561" MajorTopicYN="N">Membrane Fusion</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009976" MajorTopicYN="N">Orthomyxoviridae Infections</DescriptorName>
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<MeshHeading><DescriptorName UI="D011487" MajorTopicYN="N">Protein Conformation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName>
</MeshHeading>
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<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Conformational change</Keyword>
<Keyword MajorTopicYN="Y">Hemagglutinin stem</Keyword>
<Keyword MajorTopicYN="Y">Influenza virus</Keyword>
<Keyword MajorTopicYN="Y">Membrane fusion</Keyword>
<Keyword MajorTopicYN="Y">Neutralizing activities</Keyword>
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<Month>11</Month>
<Day>16</Day>
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<PubMedPubDate PubStatus="revised"><Year>2016</Year>
<Month>02</Month>
<Day>03</Day>
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<PubMedPubDate PubStatus="accepted"><Year>2016</Year>
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</country>
<region><li>Jilin</li>
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<settlement><li>Changchun</li>
</settlement>
<orgName><li>Université de Jilin</li>
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<tree><country name="République populaire de Chine"><region name="Jilin"><name sortKey="Gong, Xin" sort="Gong, Xin" uniqKey="Gong X" first="Xin" last="Gong">Xin Gong</name>
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<name sortKey="Guan, Shanshan" sort="Guan, Shanshan" uniqKey="Guan S" first="Shanshan" last="Guan">Shanshan Guan</name>
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<name sortKey="Jiang, Chunlai" sort="Jiang, Chunlai" uniqKey="Jiang C" first="Chunlai" last="Jiang">Chunlai Jiang</name>
<name sortKey="Kong, Wei" sort="Kong, Wei" uniqKey="Kong W" first="Wei" last="Kong">Wei Kong</name>
<name sortKey="Kuai, Ziyu" sort="Kuai, Ziyu" uniqKey="Kuai Z" first="Ziyu" last="Kuai">Ziyu Kuai</name>
<name sortKey="Shan, Yaming" sort="Shan, Yaming" uniqKey="Shan Y" first="Yaming" last="Shan">Yaming Shan</name>
<name sortKey="Shi, Yuhua" sort="Shi, Yuhua" uniqKey="Shi Y" first="Yuhua" last="Shi">Yuhua Shi</name>
<name sortKey="Wang, Song" sort="Wang, Song" uniqKey="Wang S" first="Song" last="Wang">Song Wang</name>
<name sortKey="Yang, Lan" sort="Yang, Lan" uniqKey="Yang L" first="Lan" last="Yang">Lan Yang</name>
<name sortKey="Yin, He" sort="Yin, He" uniqKey="Yin H" first="He" last="Yin">He Yin</name>
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Conserved stem fragment from H3 influenza hemagglutinin elicits cross-clade neutralizing antibodies through stalk-targeted blocking of conformational change during membrane fusion.

Conserved stem fragment from H3 influenza hemagglutinin elicits cross-clade neutralizing antibodies through stalk-targeted blocking of conformational change during membrane fusion.

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