Co-degradation of interferon signaling factor DDX3 by PB1-F2 as a basis for high virulence of 1918 pandemic influenza.
Identifieur interne : 000161 ( PubMed/Checkpoint ); précédent : 000160; suivant : 000162Co-degradation of interferon signaling factor DDX3 by PB1-F2 as a basis for high virulence of 1918 pandemic influenza.
Auteurs : Eun-Sook Park [Corée du Sud] ; Young Ho Byun [Corée du Sud] ; Soree Park [Corée du Sud] ; Yo Han Jang [Corée du Sud] ; Woo-Ry Han [Corée du Sud] ; Juhee Won [Corée du Sud] ; Kyung Cho Cho [Corée du Sud] ; Doo Hyun Kim [Corée du Sud] ; Ah Ram Lee [Corée du Sud] ; Gu-Choul Shin [Corée du Sud] ; Yong Kwang Park [Corée du Sud] ; Hong Seok Kang [Corée du Sud] ; Heewoo Sim [Corée du Sud] ; Yea Na Ha [Corée du Sud] ; Byeongjune Jae [Corée du Sud] ; Ahyun Son [Corée du Sud] ; Paul Kim [Corée du Sud] ; Jieun Yu [Corée du Sud] ; Hye-Min Lee [Corée du Sud] ; Sun-Bin Kwon [Corée du Sud] ; Kwang Pyo Kim [Corée du Sud] ; Seung-Hyun Lee [Corée du Sud] ; Yeong-Min Park [Corée du Sud] ; Baik L. Seong [Corée du Sud] ; Kyun-Hwan Kim [Corée du Sud]Source :
- The EMBO journal [ 1460-2075 ] ; 2019.
Descripteurs français
- KwdFr :
- Animaux, Cellules A549, Cellules HEK293, Cellules U937, Cellules rénales canines Madin-Darby, Chiens, DEAD-box RNA helicases (métabolisme), Femelle, Grippe humaine (histoire), Grippe humaine (virologie), Grippe humaine (épidémiologie), Histoire du 20ème siècle, Humains, Interférons (métabolisme), Orthomyxoviridae (métabolisme), Orthomyxoviridae (pathogénicité), Pandémies, Protéines virales (métabolisme), Protéines virales (physiologie), Protéolyse, Souris, Souris de lignée BALB C, Transduction du signal, Virulence (physiologie).
- MESH :
- histoire : Grippe humaine.
- métabolisme : DEAD-box RNA helicases, Interférons, Orthomyxoviridae, Protéines virales.
- pathogénicité : Orthomyxoviridae.
- physiologie : Protéines virales, Virulence.
- virologie : Grippe humaine.
- épidémiologie : Grippe humaine.
- Animaux, Cellules A549, Cellules HEK293, Cellules U937, Cellules rénales canines Madin-Darby, Chiens, Femelle, Histoire du 20ème siècle, Humains, Pandémies, Protéolyse, Souris, Souris de lignée BALB C, Transduction du signal.
English descriptors
- KwdEn :
- A549 Cells, Animals, DEAD-box RNA Helicases (metabolism), Dogs, Female, HEK293 Cells, History, 20th Century, Humans, Influenza, Human (epidemiology), Influenza, Human (history), Influenza, Human (virology), Interferons (metabolism), Madin Darby Canine Kidney Cells, Mice, Mice, Inbred BALB C, Orthomyxoviridae (metabolism), Orthomyxoviridae (pathogenicity), Pandemics, Proteolysis, Signal Transduction, U937 Cells, Viral Proteins (metabolism), Viral Proteins (physiology), Virulence (physiology).
- MESH :
- chemical , metabolism : DEAD-box RNA Helicases, Interferons, Viral Proteins.
- epidemiology : Influenza, Human.
- history : Influenza, Human.
- metabolism : Orthomyxoviridae.
- pathogenicity : Orthomyxoviridae.
- chemical , physiology : Viral Proteins, Virulence.
- virology : Influenza, Human.
- A549 Cells, Animals, Dogs, Female, HEK293 Cells, History, 20th Century, Humans, Madin Darby Canine Kidney Cells, Mice, Mice, Inbred BALB C, Pandemics, Proteolysis, Signal Transduction, U937 Cells.
Abstract
The multifunctional influenza virus protein PB1-F2 plays several roles in deregulation of host innate immune responses and is a known immunopathology enhancer of the 1918 influenza pandemic. Here, we show that the 1918 PB1-F2 protein not only interferes with the mitochondria-dependent pathway of type I interferon (IFN) signaling, but also acquired a novel IFN antagonist function by targeting the DEAD-box helicase DDX3, a key downstream mediator in antiviral interferon signaling, toward proteasome-dependent degradation. Interactome analysis revealed that 1918 PB1-F2, but not PR8 PB1-F2, binds to DDX3 and causes its co-degradation. Consistent with intrinsic protein instability as basis for this gain-of-function, internal structural disorder is associated with the unique cytotoxic sequences of the 1918 PB1-F2 protein. Infusing mice with recombinant DDX3 protein completely rescued them from lethal infection with the 1918 PB1-F2-producing virus. Alongside NS1 protein, 1918 PB1-F2 therefore constitutes a potent IFN antagonist causative for the severe pathogenicity of the 1918 influenza strain. Our identification of molecular determinants of pathogenesis should be useful for the future design of new antiviral strategies against influenza pandemics.
DOI: 10.15252/embj.201899475
PubMed: 30979777
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:30979777Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Co-degradation of interferon signaling factor DDX3 by PB1-F2 as a basis for high virulence of 1918 pandemic influenza.</title>
<author><name sortKey="Park, Eun Sook" sort="Park, Eun Sook" uniqKey="Park E" first="Eun-Sook" last="Park">Eun-Sook Park</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Byun, Young Ho" sort="Byun, Young Ho" uniqKey="Byun Y" first="Young Ho" last="Byun">Young Ho Byun</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Park, Soree" sort="Park, Soree" uniqKey="Park S" first="Soree" last="Park">Soree Park</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Jang, Yo Han" sort="Jang, Yo Han" uniqKey="Jang Y" first="Yo Han" last="Jang">Yo Han Jang</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Han, Woo Ry" sort="Han, Woo Ry" uniqKey="Han W" first="Woo-Ry" last="Han">Woo-Ry Han</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Won, Juhee" sort="Won, Juhee" uniqKey="Won J" first="Juhee" last="Won">Juhee Won</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Cho, Kyung Cho" sort="Cho, Kyung Cho" uniqKey="Cho K" first="Kyung Cho" last="Cho">Kyung Cho Cho</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Applied Chemistry, Kyung Hee University, Yongin, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Applied Chemistry, Kyung Hee University, Yongin</wicri:regionArea>
<wicri:noRegion>Yongin</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Doo Hyun" sort="Kim, Doo Hyun" uniqKey="Kim D" first="Doo Hyun" last="Kim">Doo Hyun Kim</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lee, Ah Ram" sort="Lee, Ah Ram" uniqKey="Lee A" first="Ah Ram" last="Lee">Ah Ram Lee</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Shin, Gu Choul" sort="Shin, Gu Choul" uniqKey="Shin G" first="Gu-Choul" last="Shin">Gu-Choul Shin</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Park, Yong Kwang" sort="Park, Yong Kwang" uniqKey="Park Y" first="Yong Kwang" last="Park">Yong Kwang Park</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kang, Hong Seok" sort="Kang, Hong Seok" uniqKey="Kang H" first="Hong Seok" last="Kang">Hong Seok Kang</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sim, Heewoo" sort="Sim, Heewoo" uniqKey="Sim H" first="Heewoo" last="Sim">Heewoo Sim</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ha, Yea Na" sort="Ha, Yea Na" uniqKey="Ha Y" first="Yea Na" last="Ha">Yea Na Ha</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Jae, Byeongjune" sort="Jae, Byeongjune" uniqKey="Jae B" first="Byeongjune" last="Jae">Byeongjune Jae</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Son, Ahyun" sort="Son, Ahyun" uniqKey="Son A" first="Ahyun" last="Son">Ahyun Son</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kim, Paul" sort="Kim, Paul" uniqKey="Kim P" first="Paul" last="Kim">Paul Kim</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yu, Jieun" sort="Yu, Jieun" uniqKey="Yu J" first="Jieun" last="Yu">Jieun Yu</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lee, Hye Min" sort="Lee, Hye Min" uniqKey="Lee H" first="Hye-Min" last="Lee">Hye-Min Lee</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kwon, Sun Bin" sort="Kwon, Sun Bin" uniqKey="Kwon S" first="Sun-Bin" last="Kwon">Sun-Bin Kwon</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kim, Kwang Pyo" sort="Kim, Kwang Pyo" uniqKey="Kim K" first="Kwang Pyo" last="Kim">Kwang Pyo Kim</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Applied Chemistry, Kyung Hee University, Yongin, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Applied Chemistry, Kyung Hee University, Yongin</wicri:regionArea>
<wicri:noRegion>Yongin</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lee, Seung Hyun" sort="Lee, Seung Hyun" uniqKey="Lee S" first="Seung-Hyun" last="Lee">Seung-Hyun Lee</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Microbiology, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Microbiology, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Park, Yeong Min" sort="Park, Yeong Min" uniqKey="Park Y" first="Yeong-Min" last="Park">Yeong-Min Park</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratory of Dendritic Cell Differentiation and Regulation, Department of Immunology, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Laboratory of Dendritic Cell Differentiation and Regulation, Department of Immunology, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Seong, Baik L" sort="Seong, Baik L" uniqKey="Seong B" first="Baik L" last="Seong">Baik L. Seong</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea blseong@yonsei.ac.kr khkim10@kku.ac.kr.</nlm:affiliation>
<country wicri:rule="url">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kim, Kyun Hwan" sort="Kim, Kyun Hwan" uniqKey="Kim K" first="Kyun-Hwan" last="Kim">Kyun-Hwan Kim</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea blseong@yonsei.ac.kr khkim10@kku.ac.kr.</nlm:affiliation>
<country wicri:rule="url">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2019">2019</date>
<idno type="RBID">pubmed:30979777</idno>
<idno type="pmid">30979777</idno>
<idno type="doi">10.15252/embj.201899475</idno>
<idno type="wicri:Area/PubMed/Corpus">000159</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000159</idno>
<idno type="wicri:Area/PubMed/Curation">000159</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000159</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000161</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000161</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Co-degradation of interferon signaling factor DDX3 by PB1-F2 as a basis for high virulence of 1918 pandemic influenza.</title>
<author><name sortKey="Park, Eun Sook" sort="Park, Eun Sook" uniqKey="Park E" first="Eun-Sook" last="Park">Eun-Sook Park</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Byun, Young Ho" sort="Byun, Young Ho" uniqKey="Byun Y" first="Young Ho" last="Byun">Young Ho Byun</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Park, Soree" sort="Park, Soree" uniqKey="Park S" first="Soree" last="Park">Soree Park</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Jang, Yo Han" sort="Jang, Yo Han" uniqKey="Jang Y" first="Yo Han" last="Jang">Yo Han Jang</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Han, Woo Ry" sort="Han, Woo Ry" uniqKey="Han W" first="Woo-Ry" last="Han">Woo-Ry Han</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Won, Juhee" sort="Won, Juhee" uniqKey="Won J" first="Juhee" last="Won">Juhee Won</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Cho, Kyung Cho" sort="Cho, Kyung Cho" uniqKey="Cho K" first="Kyung Cho" last="Cho">Kyung Cho Cho</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Applied Chemistry, Kyung Hee University, Yongin, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Applied Chemistry, Kyung Hee University, Yongin</wicri:regionArea>
<wicri:noRegion>Yongin</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Doo Hyun" sort="Kim, Doo Hyun" uniqKey="Kim D" first="Doo Hyun" last="Kim">Doo Hyun Kim</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lee, Ah Ram" sort="Lee, Ah Ram" uniqKey="Lee A" first="Ah Ram" last="Lee">Ah Ram Lee</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Shin, Gu Choul" sort="Shin, Gu Choul" uniqKey="Shin G" first="Gu-Choul" last="Shin">Gu-Choul Shin</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Park, Yong Kwang" sort="Park, Yong Kwang" uniqKey="Park Y" first="Yong Kwang" last="Park">Yong Kwang Park</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kang, Hong Seok" sort="Kang, Hong Seok" uniqKey="Kang H" first="Hong Seok" last="Kang">Hong Seok Kang</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sim, Heewoo" sort="Sim, Heewoo" uniqKey="Sim H" first="Heewoo" last="Sim">Heewoo Sim</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ha, Yea Na" sort="Ha, Yea Na" uniqKey="Ha Y" first="Yea Na" last="Ha">Yea Na Ha</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Jae, Byeongjune" sort="Jae, Byeongjune" uniqKey="Jae B" first="Byeongjune" last="Jae">Byeongjune Jae</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Son, Ahyun" sort="Son, Ahyun" uniqKey="Son A" first="Ahyun" last="Son">Ahyun Son</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kim, Paul" sort="Kim, Paul" uniqKey="Kim P" first="Paul" last="Kim">Paul Kim</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yu, Jieun" sort="Yu, Jieun" uniqKey="Yu J" first="Jieun" last="Yu">Jieun Yu</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lee, Hye Min" sort="Lee, Hye Min" uniqKey="Lee H" first="Hye-Min" last="Lee">Hye-Min Lee</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kwon, Sun Bin" sort="Kwon, Sun Bin" uniqKey="Kwon S" first="Sun-Bin" last="Kwon">Sun-Bin Kwon</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kim, Kwang Pyo" sort="Kim, Kwang Pyo" uniqKey="Kim K" first="Kwang Pyo" last="Kim">Kwang Pyo Kim</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Applied Chemistry, Kyung Hee University, Yongin, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Applied Chemistry, Kyung Hee University, Yongin</wicri:regionArea>
<wicri:noRegion>Yongin</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lee, Seung Hyun" sort="Lee, Seung Hyun" uniqKey="Lee S" first="Seung-Hyun" last="Lee">Seung-Hyun Lee</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Microbiology, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Department of Microbiology, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Park, Yeong Min" sort="Park, Yeong Min" uniqKey="Park Y" first="Yeong-Min" last="Park">Yeong-Min Park</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratory of Dendritic Cell Differentiation and Regulation, Department of Immunology, School of Medicine, Konkuk University, Seoul, Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Laboratory of Dendritic Cell Differentiation and Regulation, Department of Immunology, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Seong, Baik L" sort="Seong, Baik L" uniqKey="Seong B" first="Baik L" last="Seong">Baik L. Seong</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea blseong@yonsei.ac.kr khkim10@kku.ac.kr.</nlm:affiliation>
<country wicri:rule="url">Corée du Sud</country>
<wicri:regionArea>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kim, Kyun Hwan" sort="Kim, Kyun Hwan" uniqKey="Kim K" first="Kyun-Hwan" last="Kim">Kyun-Hwan Kim</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea blseong@yonsei.ac.kr khkim10@kku.ac.kr.</nlm:affiliation>
<country wicri:rule="url">Corée du Sud</country>
<wicri:regionArea>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">The EMBO journal</title>
<idno type="eISSN">1460-2075</idno>
<imprint><date when="2019" type="published">2019</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>A549 Cells</term>
<term>Animals</term>
<term>DEAD-box RNA Helicases (metabolism)</term>
<term>Dogs</term>
<term>Female</term>
<term>HEK293 Cells</term>
<term>History, 20th Century</term>
<term>Humans</term>
<term>Influenza, Human (epidemiology)</term>
<term>Influenza, Human (history)</term>
<term>Influenza, Human (virology)</term>
<term>Interferons (metabolism)</term>
<term>Madin Darby Canine Kidney Cells</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Orthomyxoviridae (metabolism)</term>
<term>Orthomyxoviridae (pathogenicity)</term>
<term>Pandemics</term>
<term>Proteolysis</term>
<term>Signal Transduction</term>
<term>U937 Cells</term>
<term>Viral Proteins (metabolism)</term>
<term>Viral Proteins (physiology)</term>
<term>Virulence (physiology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Cellules A549</term>
<term>Cellules HEK293</term>
<term>Cellules U937</term>
<term>Cellules rénales canines Madin-Darby</term>
<term>Chiens</term>
<term>DEAD-box RNA helicases (métabolisme)</term>
<term>Femelle</term>
<term>Grippe humaine (histoire)</term>
<term>Grippe humaine (virologie)</term>
<term>Grippe humaine (épidémiologie)</term>
<term>Histoire du 20ème siècle</term>
<term>Humains</term>
<term>Interférons (métabolisme)</term>
<term>Orthomyxoviridae (métabolisme)</term>
<term>Orthomyxoviridae (pathogénicité)</term>
<term>Pandémies</term>
<term>Protéines virales (métabolisme)</term>
<term>Protéines virales (physiologie)</term>
<term>Protéolyse</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Transduction du signal</term>
<term>Virulence (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DEAD-box RNA Helicases</term>
<term>Interferons</term>
<term>Viral Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Influenza, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="histoire" xml:lang="fr"><term>Grippe humaine</term>
</keywords>
<keywords scheme="MESH" qualifier="history" xml:lang="en"><term>Influenza, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Orthomyxoviridae</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>DEAD-box RNA helicases</term>
<term>Interférons</term>
<term>Orthomyxoviridae</term>
<term>Protéines virales</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Orthomyxoviridae</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Orthomyxoviridae</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Protéines virales</term>
<term>Virulence</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Viral Proteins</term>
<term>Virulence</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Grippe humaine</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>A549 Cells</term>
<term>Animals</term>
<term>Dogs</term>
<term>Female</term>
<term>HEK293 Cells</term>
<term>History, 20th Century</term>
<term>Humans</term>
<term>Madin Darby Canine Kidney Cells</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Pandemics</term>
<term>Proteolysis</term>
<term>Signal Transduction</term>
<term>U937 Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules A549</term>
<term>Cellules HEK293</term>
<term>Cellules U937</term>
<term>Cellules rénales canines Madin-Darby</term>
<term>Chiens</term>
<term>Femelle</term>
<term>Histoire du 20ème siècle</term>
<term>Humains</term>
<term>Pandémies</term>
<term>Protéolyse</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Transduction du signal</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The multifunctional influenza virus protein PB1-F2 plays several roles in deregulation of host innate immune responses and is a known immunopathology enhancer of the 1918 influenza pandemic. Here, we show that the 1918 PB1-F2 protein not only interferes with the mitochondria-dependent pathway of type I interferon (IFN) signaling, but also acquired a novel IFN antagonist function by targeting the DEAD-box helicase DDX3, a key downstream mediator in antiviral interferon signaling, toward proteasome-dependent degradation. Interactome analysis revealed that 1918 PB1-F2, but not PR8 PB1-F2, binds to DDX3 and causes its co-degradation. Consistent with intrinsic protein instability as basis for this gain-of-function, internal structural disorder is associated with the unique cytotoxic sequences of the 1918 PB1-F2 protein. Infusing mice with recombinant DDX3 protein completely rescued them from lethal infection with the 1918 PB1-F2-producing virus. Alongside NS1 protein, 1918 PB1-F2 therefore constitutes a potent IFN antagonist causative for the severe pathogenicity of the 1918 influenza strain. Our identification of molecular determinants of pathogenesis should be useful for the future design of new antiviral strategies against influenza pandemics.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30979777</PMID>
<DateCompleted><Year>2020</Year>
<Month>01</Month>
<Day>06</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>05</Month>
<Day>16</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1460-2075</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>38</Volume>
<Issue>10</Issue>
<PubDate><Year>2019</Year>
<Month>05</Month>
<Day>15</Day>
</PubDate>
</JournalIssue>
<Title>The EMBO journal</Title>
<ISOAbbreviation>EMBO J.</ISOAbbreviation>
</Journal>
<ArticleTitle>Co-degradation of interferon signaling factor DDX3 by PB1-F2 as a basis for high virulence of 1918 pandemic influenza.</ArticleTitle>
<ELocationID EIdType="pii" ValidYN="Y">e99475</ELocationID>
<ELocationID EIdType="doi" ValidYN="Y">10.15252/embj.201899475</ELocationID>
<Abstract><AbstractText>The multifunctional influenza virus protein PB1-F2 plays several roles in deregulation of host innate immune responses and is a known immunopathology enhancer of the 1918 influenza pandemic. Here, we show that the 1918 PB1-F2 protein not only interferes with the mitochondria-dependent pathway of type I interferon (IFN) signaling, but also acquired a novel IFN antagonist function by targeting the DEAD-box helicase DDX3, a key downstream mediator in antiviral interferon signaling, toward proteasome-dependent degradation. Interactome analysis revealed that 1918 PB1-F2, but not PR8 PB1-F2, binds to DDX3 and causes its co-degradation. Consistent with intrinsic protein instability as basis for this gain-of-function, internal structural disorder is associated with the unique cytotoxic sequences of the 1918 PB1-F2 protein. Infusing mice with recombinant DDX3 protein completely rescued them from lethal infection with the 1918 PB1-F2-producing virus. Alongside NS1 protein, 1918 PB1-F2 therefore constitutes a potent IFN antagonist causative for the severe pathogenicity of the 1918 influenza strain. Our identification of molecular determinants of pathogenesis should be useful for the future design of new antiviral strategies against influenza pandemics.</AbstractText>
<CopyrightInformation>© 2019 The Authors.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Park</LastName>
<ForeName>Eun-Sook</ForeName>
<Initials>ES</Initials>
<AffiliationInfo><Affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>KU Open Innovation Center, Konkuk University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Byun</LastName>
<ForeName>Young Ho</ForeName>
<Initials>YH</Initials>
<Identifier Source="ORCID">0000-0001-9306-9485</Identifier>
<AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Vaccine Translational Research Center, Yonsei University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Park</LastName>
<ForeName>Soree</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jang</LastName>
<ForeName>Yo Han</ForeName>
<Initials>YH</Initials>
<AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Vaccine Translational Research Center, Yonsei University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Han</LastName>
<ForeName>Woo-Ry</ForeName>
<Initials>WR</Initials>
<AffiliationInfo><Affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Won</LastName>
<ForeName>Juhee</ForeName>
<Initials>J</Initials>
<Identifier Source="ORCID">0000-0002-5757-3745</Identifier>
<AffiliationInfo><Affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Cho</LastName>
<ForeName>Kyung Cho</ForeName>
<Initials>KC</Initials>
<Identifier Source="ORCID">0000-0003-0483-9543</Identifier>
<AffiliationInfo><Affiliation>Department of Applied Chemistry, Kyung Hee University, Yongin, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kim</LastName>
<ForeName>Doo Hyun</ForeName>
<Initials>DH</Initials>
<AffiliationInfo><Affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lee</LastName>
<ForeName>Ah Ram</ForeName>
<Initials>AR</Initials>
<AffiliationInfo><Affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Shin</LastName>
<ForeName>Gu-Choul</ForeName>
<Initials>GC</Initials>
<Identifier Source="ORCID">0000-0001-8200-9700</Identifier>
<AffiliationInfo><Affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Park</LastName>
<ForeName>Yong Kwang</ForeName>
<Initials>YK</Initials>
<AffiliationInfo><Affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kang</LastName>
<ForeName>Hong Seok</ForeName>
<Initials>HS</Initials>
<AffiliationInfo><Affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sim</LastName>
<ForeName>Heewoo</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ha</LastName>
<ForeName>Yea Na</ForeName>
<Initials>YN</Initials>
<AffiliationInfo><Affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jae</LastName>
<ForeName>Byeongjune</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Son</LastName>
<ForeName>Ahyun</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kim</LastName>
<ForeName>Paul</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Vaccine Translational Research Center, Yonsei University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yu</LastName>
<ForeName>Jieun</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lee</LastName>
<ForeName>Hye-Min</ForeName>
<Initials>HM</Initials>
<AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kwon</LastName>
<ForeName>Sun-Bin</ForeName>
<Initials>SB</Initials>
<AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kim</LastName>
<ForeName>Kwang Pyo</ForeName>
<Initials>KP</Initials>
<AffiliationInfo><Affiliation>Department of Applied Chemistry, Kyung Hee University, Yongin, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lee</LastName>
<ForeName>Seung-Hyun</ForeName>
<Initials>SH</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, School of Medicine, Konkuk University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Park</LastName>
<ForeName>Yeong-Min</ForeName>
<Initials>YM</Initials>
<AffiliationInfo><Affiliation>Laboratory of Dendritic Cell Differentiation and Regulation, Department of Immunology, School of Medicine, Konkuk University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Seong</LastName>
<ForeName>Baik L</ForeName>
<Initials>BL</Initials>
<Identifier Source="ORCID">0000-0002-7301-082X</Identifier>
<AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea blseong@yonsei.ac.kr khkim10@kku.ac.kr.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Vaccine Translational Research Center, Yonsei University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kim</LastName>
<ForeName>Kyun-Hwan</ForeName>
<Initials>KH</Initials>
<Identifier Source="ORCID">0000-0001-5266-072X</Identifier>
<AffiliationInfo><Affiliation>Department of Pharmacology, Center for Cancer Research and Diagnostic Medicine, IBST, School of Medicine, Konkuk University, Seoul, Korea blseong@yonsei.ac.kr khkim10@kku.ac.kr.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>KU Open Innovation Center, Konkuk University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Research Institute of Medical Science, Konkuk University, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016456">Historical Article</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2019</Year>
<Month>04</Month>
<Day>12</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>EMBO J</MedlineTA>
<NlmUniqueID>8208664</NlmUniqueID>
<ISSNLinking>0261-4189</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C442816">PB1-F2 protein, Influenza A virus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9008-11-1</RegistryNumber>
<NameOfSubstance UI="D007372">Interferons</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.6.1.-</RegistryNumber>
<NameOfSubstance UI="C119332">DDX3X protein, human</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.6.4.13</RegistryNumber>
<NameOfSubstance UI="D053487">DEAD-box RNA Helicases</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000072283" MajorTopicYN="N">A549 Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D053487" MajorTopicYN="N">DEAD-box RNA Helicases</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004285" MajorTopicYN="N">Dogs</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D057809" MajorTopicYN="N">HEK293 Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D049673" MajorTopicYN="N">History, 20th Century</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName>
<QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName>
<QualifierName UI="Q000266" MajorTopicYN="N">history</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007372" MajorTopicYN="N">Interferons</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D061985" MajorTopicYN="N">Madin Darby Canine Kidney Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009975" MajorTopicYN="N">Orthomyxoviridae</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D058873" MajorTopicYN="N">Pandemics</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D059748" MajorTopicYN="N">Proteolysis</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020298" MajorTopicYN="N">U937 Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">1918 pandemic</Keyword>
<Keyword MajorTopicYN="Y">DDX3</Keyword>
<Keyword MajorTopicYN="Y">influenza PB1‐F2</Keyword>
<Keyword MajorTopicYN="Y">type I interferon signaling</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year>
<Month>04</Month>
<Day>17</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2019</Year>
<Month>03</Month>
<Day>06</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2019</Year>
<Month>03</Month>
<Day>08</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2019</Year>
<Month>4</Month>
<Day>14</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2020</Year>
<Month>1</Month>
<Day>7</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2019</Year>
<Month>4</Month>
<Day>14</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">30979777</ArticleId>
<ArticleId IdType="pii">embj.201899475</ArticleId>
<ArticleId IdType="doi">10.15252/embj.201899475</ArticleId>
<ArticleId IdType="pmc">PMC6518015</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>PLoS Pathog. 2008 Aug 01;4(8):e1000115</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18670648</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2008 Oct;89(Pt 10):2359-2376</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18796704</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Med. 2001 Dec;7(12):1306-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11726970</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2014 Jan;88(1):503-15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24173220</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2011 Jan;85(2):652-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21084483</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2016 Dec 1;21(48):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27934581</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2003 Jul;77(13):7214-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12805420</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2010 Apr 23;285(17):13233-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20172856</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2007 Feb 2;315(5812):655-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17272724</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Host Microbe. 2007 Oct 11;2(4):207-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18005736</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2012 Aug;86(16):8359-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22674996</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Biol. 2013 May;33(10):2004-15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23478265</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochim Biophys Acta. 2013 Aug;1829(8):854-65</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23567047</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Immunol. 2015 Aug 19;6:419</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26347742</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 2014 Mar 20;426(6):1322-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24184279</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 2010 Mar 19;140(6):805-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20303872</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2006 May 12;312(5775):879-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16690858</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Microbiol. 2017 Apr 21;8:692</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28484439</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2013 Jun 5;440(2):97-104</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23545143</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2006 Aug;80(16):7976-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16873254</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochim Biophys Acta. 2010 Apr;1804(4):996-1010</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20100603</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Biotechnol. 2007 Apr;25(4):433-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17420751</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2016 Jan 20;90(7):3661-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26792746</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2008 Jul 16;3(7):e2677</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18628952</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Med. 2002 Sep;8(9):950-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12195436</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2011 Jun;7(6):e1002067</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21695240</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteins. 2005;61 Suppl 7:176-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16187360</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Hist Philos Life Sci. 1991;13(2):223-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1724803</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2010 Jul 22;6(7):e1001014</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20661425</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2013 Oct 02;8(10):e75043</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24098364</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Mol Sci. 2018 Oct 03;19(10):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30282926</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Microbiol. 2004 Nov;2(11):909-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15494747</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Host Microbe. 2007 Oct 11;2(4):240-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18005742</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2007 Jan 18;445(7125):319-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17230189</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2006;1(6):2856-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17406544</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2016 Jan 8;291(2):739-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26601953</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Med. 2001 Dec;7(12):1286-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11726965</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>mBio. 2018 Jan 16;9(1):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29339427</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>RNA Biol. 2009 Jan-Mar;6(1):21-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19106620</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2009 Oct;5(10):e1000604</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19798428</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2005 Oct 7;310(5745):77-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16210530</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10736-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12149435</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Corée du Sud</li>
</country>
<region><li>Région capitale de Séoul</li>
</region>
<settlement><li>Séoul</li>
</settlement>
</list>
<tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Park, Eun Sook" sort="Park, Eun Sook" uniqKey="Park E" first="Eun-Sook" last="Park">Eun-Sook Park</name>
</region>
<name sortKey="Byun, Young Ho" sort="Byun, Young Ho" uniqKey="Byun Y" first="Young Ho" last="Byun">Young Ho Byun</name>
<name sortKey="Cho, Kyung Cho" sort="Cho, Kyung Cho" uniqKey="Cho K" first="Kyung Cho" last="Cho">Kyung Cho Cho</name>
<name sortKey="Ha, Yea Na" sort="Ha, Yea Na" uniqKey="Ha Y" first="Yea Na" last="Ha">Yea Na Ha</name>
<name sortKey="Han, Woo Ry" sort="Han, Woo Ry" uniqKey="Han W" first="Woo-Ry" last="Han">Woo-Ry Han</name>
<name sortKey="Jae, Byeongjune" sort="Jae, Byeongjune" uniqKey="Jae B" first="Byeongjune" last="Jae">Byeongjune Jae</name>
<name sortKey="Jang, Yo Han" sort="Jang, Yo Han" uniqKey="Jang Y" first="Yo Han" last="Jang">Yo Han Jang</name>
<name sortKey="Kang, Hong Seok" sort="Kang, Hong Seok" uniqKey="Kang H" first="Hong Seok" last="Kang">Hong Seok Kang</name>
<name sortKey="Kim, Doo Hyun" sort="Kim, Doo Hyun" uniqKey="Kim D" first="Doo Hyun" last="Kim">Doo Hyun Kim</name>
<name sortKey="Kim, Kwang Pyo" sort="Kim, Kwang Pyo" uniqKey="Kim K" first="Kwang Pyo" last="Kim">Kwang Pyo Kim</name>
<name sortKey="Kim, Kyun Hwan" sort="Kim, Kyun Hwan" uniqKey="Kim K" first="Kyun-Hwan" last="Kim">Kyun-Hwan Kim</name>
<name sortKey="Kim, Paul" sort="Kim, Paul" uniqKey="Kim P" first="Paul" last="Kim">Paul Kim</name>
<name sortKey="Kwon, Sun Bin" sort="Kwon, Sun Bin" uniqKey="Kwon S" first="Sun-Bin" last="Kwon">Sun-Bin Kwon</name>
<name sortKey="Lee, Ah Ram" sort="Lee, Ah Ram" uniqKey="Lee A" first="Ah Ram" last="Lee">Ah Ram Lee</name>
<name sortKey="Lee, Hye Min" sort="Lee, Hye Min" uniqKey="Lee H" first="Hye-Min" last="Lee">Hye-Min Lee</name>
<name sortKey="Lee, Seung Hyun" sort="Lee, Seung Hyun" uniqKey="Lee S" first="Seung-Hyun" last="Lee">Seung-Hyun Lee</name>
<name sortKey="Park, Soree" sort="Park, Soree" uniqKey="Park S" first="Soree" last="Park">Soree Park</name>
<name sortKey="Park, Yeong Min" sort="Park, Yeong Min" uniqKey="Park Y" first="Yeong-Min" last="Park">Yeong-Min Park</name>
<name sortKey="Park, Yong Kwang" sort="Park, Yong Kwang" uniqKey="Park Y" first="Yong Kwang" last="Park">Yong Kwang Park</name>
<name sortKey="Seong, Baik L" sort="Seong, Baik L" uniqKey="Seong B" first="Baik L" last="Seong">Baik L. Seong</name>
<name sortKey="Shin, Gu Choul" sort="Shin, Gu Choul" uniqKey="Shin G" first="Gu-Choul" last="Shin">Gu-Choul Shin</name>
<name sortKey="Sim, Heewoo" sort="Sim, Heewoo" uniqKey="Sim H" first="Heewoo" last="Sim">Heewoo Sim</name>
<name sortKey="Son, Ahyun" sort="Son, Ahyun" uniqKey="Son A" first="Ahyun" last="Son">Ahyun Son</name>
<name sortKey="Won, Juhee" sort="Won, Juhee" uniqKey="Won J" first="Juhee" last="Won">Juhee Won</name>
<name sortKey="Yu, Jieun" sort="Yu, Jieun" uniqKey="Yu J" first="Jieun" last="Yu">Jieun Yu</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/PandemieGrippaleV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000161 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 000161 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= PandemieGrippaleV1 |flux= PubMed |étape= Checkpoint |type= RBID |clé= pubmed:30979777 |texte= Co-degradation of interferon signaling factor DDX3 by PB1-F2 as a basis for high virulence of 1918 pandemic influenza. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:30979777" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a PandemieGrippaleV1
![]() | This area was generated with Dilib version V0.6.34. | ![]() |