PandemieGrippaleV1, Pmc, Corpus, bibRecord, 000842

Vaccination with Killed but Metabolically Active E. coli Over-expressing Hemagglutinin Elicits Neutralizing Antibodies to H1N1 Swine Origin Influenza A Virus

Identifieur interne : 000842 ( Pmc/Corpus ); précédent : 000841; suivant : 000843

Vaccination with Killed but Metabolically Active E. coli Over-expressing Hemagglutinin Elicits Neutralizing Antibodies to H1N1 Swine Origin Influenza A Virus

Auteurs : Pei-Feng Liu ; Yanhan Wang ; Yu-Tsueng Liu ; Chun-Ming Huang

Source :

Journal of nature and science [ 2377-2700 ] ; 2017.

RBID : PMC:5421401

Abstract

There is a need for a fast and simple method for vaccine production to keep up with the pace of a rapidly spreading virus in the early phases of the influenza pandemic. The use of whole viruses produced in chicken eggs or recombinant antigens purified from various expression systems has presented considerable challenges, especially with lengthy processing times. Here, we use the killed but metabolically active (KBMA) Escherichia coli (E. coli) to harbor the hemagglutinin (HA) of swine origin influenza A (H1N1) virus (S-OIV) San Diego/01/09 (SD/H1N1-S-OIV). Intranasal vaccination of mice with KBMA E. coli SD/H1N1-S-OIV HA without adding exogenous adjuvants provoked detectable neutralizing antibodies against the virus-induced hemagglutination within three weeks. Boosting vaccination enhanced the titers of neutralizing antibodies, which can decrease viral infectivity in Madin-Darby canine kidney (MDCK) cells. The antibodies were found to specifically neutralize the SD/H1N1-S-OIV-, but not seasonal influenza viruses (H1N1 and H3N2), -induced hemagglutination. The use of KBMA E. coli as an egg-free system to produce anti-influenza vaccines makes unnecessary the rigorous purification of an antigen prior to immunization, providing an alternative modality to combat influenza virus in future outbreaks.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5421401

PubMed: 28492063
PubMed Central: 5421401

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PMC:5421401

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Vaccination with Killed but Metabolically Active <italic>E. coli</italic>
 Over-expressing Hemagglutinin Elicits Neutralizing Antibodies to H1N1 Swine Origin Influenza A Virus</title>
<author><name sortKey="Liu, Pei Feng" sort="Liu, Pei Feng" uniqKey="Liu P" first="Pei-Feng" last="Liu">Pei-Feng Liu</name>
<affiliation><nlm:aff id="A1">Department of Dermatology, University of California, San Diego, San Diego, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Yanhan" sort="Wang, Yanhan" uniqKey="Wang Y" first="Yanhan" last="Wang">Yanhan Wang</name>
<affiliation><nlm:aff id="A1">Department of Dermatology, University of California, San Diego, San Diego, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liu, Yu Tsueng" sort="Liu, Yu Tsueng" uniqKey="Liu Y" first="Yu-Tsueng" last="Liu">Yu-Tsueng Liu</name>
<affiliation><nlm:aff id="A2">Division of Infectious Diseases, Department of Medicine, University of California, San Diego, USA</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A3">Moores Cancer Center, University of California, San Diego, San Diego, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Huang, Chun Ming" sort="Huang, Chun Ming" uniqKey="Huang C" first="Chun-Ming" last="Huang">Chun-Ming Huang</name>
<affiliation><nlm:aff id="A1">Department of Dermatology, University of California, San Diego, San Diego, USA</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A3">Moores Cancer Center, University of California, San Diego, San Diego, USA</nlm:aff>
</affiliation>
</author>
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<idno type="pmc">5421401</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5421401</idno>
<idno type="RBID">PMC:5421401</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Vaccination with Killed but Metabolically Active <italic>E. coli</italic>
 Over-expressing Hemagglutinin Elicits Neutralizing Antibodies to H1N1 Swine Origin Influenza A Virus</title>
<author><name sortKey="Liu, Pei Feng" sort="Liu, Pei Feng" uniqKey="Liu P" first="Pei-Feng" last="Liu">Pei-Feng Liu</name>
<affiliation><nlm:aff id="A1">Department of Dermatology, University of California, San Diego, San Diego, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Yanhan" sort="Wang, Yanhan" uniqKey="Wang Y" first="Yanhan" last="Wang">Yanhan Wang</name>
<affiliation><nlm:aff id="A1">Department of Dermatology, University of California, San Diego, San Diego, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liu, Yu Tsueng" sort="Liu, Yu Tsueng" uniqKey="Liu Y" first="Yu-Tsueng" last="Liu">Yu-Tsueng Liu</name>
<affiliation><nlm:aff id="A2">Division of Infectious Diseases, Department of Medicine, University of California, San Diego, USA</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A3">Moores Cancer Center, University of California, San Diego, San Diego, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Huang, Chun Ming" sort="Huang, Chun Ming" uniqKey="Huang C" first="Chun-Ming" last="Huang">Chun-Ming Huang</name>
<affiliation><nlm:aff id="A1">Department of Dermatology, University of California, San Diego, San Diego, USA</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A3">Moores Cancer Center, University of California, San Diego, San Diego, USA</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of nature and science</title>
<idno type="eISSN">2377-2700</idno>
<imprint><date when="2017">2017</date>
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<front><div type="abstract" xml:lang="en"><p id="P1">There is a need for a fast and simple method for vaccine production to keep up with the pace of a rapidly spreading virus in the early phases of the influenza pandemic. The use of whole viruses produced in chicken eggs or recombinant antigens purified from various expression systems has presented considerable challenges, especially with lengthy processing times. Here, we use the killed but metabolically active (KBMA) <italic>Escherichia coli</italic>
 (<italic>E. coli</italic>
) to harbor the hemagglutinin (HA) of swine origin influenza A (H1N1) virus (S-OIV) San Diego/01/09 (SD/H1N1-S-OIV). Intranasal vaccination of mice with KBMA <italic>E. coli</italic>
 SD/H1N1-S-OIV HA without adding exogenous adjuvants provoked detectable neutralizing antibodies against the virus-induced hemagglutination within three weeks. Boosting vaccination enhanced the titers of neutralizing antibodies, which can decrease viral infectivity in Madin-Darby canine kidney (MDCK) cells. The antibodies were found to specifically neutralize the SD/H1N1-S-OIV-, but not seasonal influenza viruses (H1N1 and H3N2), -induced hemagglutination. The use of KBMA <italic>E. coli</italic>
 as an egg-free system to produce anti-influenza vaccines makes unnecessary the rigorous purification of an antigen prior to immunization, providing an alternative modality to combat influenza virus in future outbreaks.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <pmc-dir>properties manuscript</pmc-dir>
  <front><journal-meta><journal-id journal-id-type="nlm-journal-id">101652055</journal-id>
<journal-id journal-id-type="pubmed-jr-id">43566</journal-id>
<journal-id journal-id-type="nlm-ta">J Nat Sci</journal-id>
<journal-id journal-id-type="iso-abbrev">J Nat Sci</journal-id>
<journal-title-group><journal-title>Journal of nature and science</journal-title>
</journal-title-group>
<issn pub-type="epub">2377-2700</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">28492063</article-id>
<article-id pub-id-type="pmc">5421401</article-id>
<article-id pub-id-type="manuscript">NIHMS853086</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Vaccination with Killed but Metabolically Active <italic>E. coli</italic>
 Over-expressing Hemagglutinin Elicits Neutralizing Antibodies to H1N1 Swine Origin Influenza A Virus</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Liu</surname>
<given-names>Pei-Feng</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Yanhan</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Liu</surname>
<given-names>Yu-Tsueng</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
<xref ref-type="aff" rid="A3">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Huang</surname>
<given-names>Chun-Ming</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref ref-type="aff" rid="A3">3</xref>
<xref rid="FN1" ref-type="author-notes">*</xref>
</contrib>
</contrib-group>
<aff id="A1"><label>1</label>
Department of Dermatology, University of California, San Diego, San Diego, USA</aff>
<aff id="A2"><label>2</label>
Division of Infectious Diseases, Department of Medicine, University of California, San Diego, USA</aff>
<aff id="A3"><label>3</label>
Moores Cancer Center, University of California, San Diego, San Diego, USA</aff>
<author-notes><corresp id="FN1"><label>*</label>
Corresponding Author: Professor, Chun-Ming Huang, Department of Dermatology, University of California, San Diego. 3525 John Hopkins Court, Rm276, San Diego, CA, 92121, USA. Tel: 858-822-4627; Fax: 858-642-1435. <email>chunming@ucsd.edu</email>
</corresp>
<fn fn-type="COI-statement" id="FN2"><p>Conflict of interest: No conflicts declared.</p>
</fn>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>20</day>
<month>3</month>
<year>2017</year>
</pub-date>
<pub-date pub-type="ppub"><month>2</month>
<year>2017</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>08</day>
<month>5</month>
<year>2017</year>
</pub-date>
<volume>3</volume>
<issue>2</issue>
<elocation-id>e317</elocation-id>
<self-uri xlink:href="http://www.jnsci.org/files/html/2017/e317.htm"></self-uri>
<abstract><p id="P1">There is a need for a fast and simple method for vaccine production to keep up with the pace of a rapidly spreading virus in the early phases of the influenza pandemic. The use of whole viruses produced in chicken eggs or recombinant antigens purified from various expression systems has presented considerable challenges, especially with lengthy processing times. Here, we use the killed but metabolically active (KBMA) <italic>Escherichia coli</italic>
 (<italic>E. coli</italic>
) to harbor the hemagglutinin (HA) of swine origin influenza A (H1N1) virus (S-OIV) San Diego/01/09 (SD/H1N1-S-OIV). Intranasal vaccination of mice with KBMA <italic>E. coli</italic>
 SD/H1N1-S-OIV HA without adding exogenous adjuvants provoked detectable neutralizing antibodies against the virus-induced hemagglutination within three weeks. Boosting vaccination enhanced the titers of neutralizing antibodies, which can decrease viral infectivity in Madin-Darby canine kidney (MDCK) cells. The antibodies were found to specifically neutralize the SD/H1N1-S-OIV-, but not seasonal influenza viruses (H1N1 and H3N2), -induced hemagglutination. The use of KBMA <italic>E. coli</italic>
 as an egg-free system to produce anti-influenza vaccines makes unnecessary the rigorous purification of an antigen prior to immunization, providing an alternative modality to combat influenza virus in future outbreaks.</p>
</abstract>
<kwd-group><kwd>H1N1 swine origin influenza A virus</kwd>
<kwd>Hemagglutinin</kwd>
<kwd>KBMA</kwd>
<kwd>microbe based-vaccine</kwd>
<kwd>Intranasal vaccine</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>

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Vaccination with Killed but Metabolically Active E. coli Over-expressing Hemagglutinin Elicits Neutralizing Antibodies to H1N1 Swine Origin Influenza A Virus

Vaccination with Killed but Metabolically Active E. coli Over-expressing Hemagglutinin Elicits Neutralizing Antibodies to H1N1 Swine Origin Influenza A Virus

Source :

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki