Generation of human neutralizing monoclonal antibodies against the 2009 pandemic H1N1 virus from peripheral blood memory B lymphocytes
Identifieur interne : 000001 ( Pmc/Corpus ); précédent : 000000; suivant : 000002Generation of human neutralizing monoclonal antibodies against the 2009 pandemic H1N1 virus from peripheral blood memory B lymphocytes
Auteurs : Hao Wang ; Chi Ma ; Yanlai Lu ; Xu Ji ; Yongsheng Pang ; Fang Hua ; Lianxian Cui ; Denian Ba ; Wei HeSource :
- Cellular and Molecular Immunology [ 1672-7681 ] ; 2013.
Abstract
The 2009 H1N1 influenza pandemic demonstrated the significance of a global health threat to human beings. Although pandemic H1N1 vaccines have been rapidly developed, passive serotherapy may offer superior immediate protection against infections in children, the elderly and immune-compromised patients during an influenza pandemic. Here, we applied a novel strategy based on Epstein–Barr virus (EBV)-immortalized peripheral blood memory B cells to screen high viral neutralizing monoclonal antibodies (MAbs) from individuals vaccinated with the 2009 pandemic H1N1 vaccine PANFLU.1. Through a massive screen of 13 090 immortalized memory B-cell clones from three selected vaccinees, seven MAbs were identified with both high viral neutralizing capacities and hemagglutination inhibition (HAI) activities against the 2009 pandemic H1N1 viruses. These MAbs may have important clinical implications for passive serotherapy treatments of infected patients with severe respiratory syndrome, especially children, the elderly and immunodeficient individuals. Our successful strategy for generating high-affinity MAbs from EBV-immortalized peripheral blood memory B cells may also be applicable to other infectious or autoimmune diseases.
Url:
DOI: 10.1038/cmi.2013.25
PubMed: 23912783
PubMed Central: 4003197
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<front><journal-meta><journal-id journal-id-type="nlm-ta">Cell Mol Immunol</journal-id><journal-id journal-id-type="iso-abbrev">Cell. Mol. Immunol</journal-id><journal-title-group><journal-title>Cellular and Molecular Immunology</journal-title></journal-title-group><issn pub-type="ppub">1672-7681</issn><issn pub-type="epub">2042-0226</issn><publisher><publisher-name>Nature Publishing Group</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23912783</article-id><article-id pub-id-type="pmc">4003197</article-id><article-id pub-id-type="pii">cmi201325</article-id><article-id pub-id-type="doi">10.1038/cmi.2013.25</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Generation of human neutralizing monoclonal antibodies against the 2009 pandemic H1N1 virus from peripheral blood memory B lymphocytes</article-title><alt-title alt-title-type="running">Generation of human neutralizing Mabs against H1N1 virus</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Hao</given-names></name></contrib><contrib contrib-type="author"><name><surname>Ma</surname><given-names>Chi</given-names></name></contrib><contrib contrib-type="author"><name><surname>Lu</surname><given-names>Yanlai</given-names></name></contrib><contrib contrib-type="author"><name><surname>Ji</surname><given-names>Xu</given-names></name></contrib><contrib contrib-type="author"><name><surname>Pang</surname><given-names>Yongsheng</given-names></name></contrib><contrib contrib-type="author"><name><surname>Hua</surname><given-names>Fang</given-names></name></contrib><contrib contrib-type="author"><name><surname>Cui</surname><given-names>Lianxian</given-names></name></contrib><contrib contrib-type="author"><name><surname>Ba</surname><given-names>Denian</given-names></name></contrib><contrib contrib-type="author"><name><surname>He</surname><given-names>Wei</given-names></name></contrib><aff id="aff1"><institution>Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, State Key Laboratory of Medical Molecular Biology</institution>, Beijing,<country>China</country></aff></contrib-group><author-notes><corresp id="caf1"><label>*</label><institution>Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, State Key Laboratory of Medical Molecular Biology, 5 Dong Dan San Tiao</institution>, Beijing 100005, <country>China</country>. E-mail: <email>heweiimu@public.bta.net.cn</email></corresp></author-notes><pub-date pub-type="ppub"><month>09</month><year>2013</year></pub-date><pub-date pub-type="epub"><day>05</day><month>08</month><year>2013</year></pub-date><volume>10</volume><issue>5</issue><fpage>403</fpage><lpage>412</lpage><history><date date-type="received"><day>28</day><month>03</month><year>2013</year></date><date date-type="rev-recd"><day>13</day><month>05</month><year>2013</year></date><date date-type="accepted"><day>14</day><month>05</month><year>2013</year></date></history><permissions><copyright-statement>Copyright © 2013 Chinese Society of Immunology and The University of Science and Technology</copyright-statement><copyright-year>2013</copyright-year><copyright-holder>Chinese Society of Immunology and The University of Science and Technology</copyright-holder></permissions><abstract><p>The 2009 H1N1 influenza pandemic demonstrated the significance of a global health threat to human beings. Although pandemic H1N1 vaccines have been rapidly developed, passive serotherapy may offer superior immediate protection against infections in children, the elderly and immune-compromised patients during an influenza pandemic. Here, we applied a novel strategy based on Epstein–Barr virus (EBV)-immortalized peripheral blood memory B cells to screen high viral neutralizing monoclonal antibodies (MAbs) from individuals vaccinated with the 2009 pandemic H1N1 vaccine PANFLU.1. Through a massive screen of 13 090 immortalized memory B-cell clones from three selected vaccinees, seven MAbs were identified with both high viral neutralizing capacities and hemagglutination inhibition (HAI) activities against the 2009 pandemic H1N1 viruses. These MAbs may have important clinical implications for passive serotherapy treatments of infected patients with severe respiratory syndrome, especially children, the elderly and immunodeficient individuals. Our successful strategy for generating high-affinity MAbs from EBV-immortalized peripheral blood memory B cells may also be applicable to other infectious or autoimmune diseases.</p></abstract><kwd-group><kwd>Epstein–Barr virus</kwd><kwd>hemagglutinin</kwd><kwd>memory B cells</kwd><kwd>neutralizing monoclonal antibody</kwd><kwd>2009 pandemic influenza H1N1 virus</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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