Human HA and polymerase subunit PB2 proteins confer transmission of an avian influenza virus through the air
Identifieur interne : 000C00 ( Pmc/Checkpoint ); précédent : 000B99; suivant : 000C01Human HA and polymerase subunit PB2 proteins confer transmission of an avian influenza virus through the air
Auteurs : Neal Van Hoeven [États-Unis] ; Claudia Pappas [États-Unis] ; Jessica A. Belser [États-Unis] ; Taronna R. Maines [États-Unis] ; Hui Zeng [États-Unis] ; Adolfo García-Sastre [États-Unis] ; Ram Sasisekharan [États-Unis] ; Jacqueline M. Katz [États-Unis] ; Terrence M. Tumpey [États-Unis]Source :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2009.
Abstract
The influenza virus genes that confer efficient transmission of epidemic and pandemic strains in humans have not been identified. The rapid spread and severe disease caused by the 1918 influenza pandemic virus makes it an ideal virus to study the transmissibility of potentially pandemic influenza strains. Here, we used a series of human 1918-avian H1N1 influenza reassortant viruses to identify the genetic determinants that govern airborne transmission of avian influenza viruses. We have demonstrated that the 1918 HA gene was necessary for efficient direct contact transmission, but did not allow respiratory droplet transmission between ferrets of an avian influenza virus possessing an avian polymerase subunit PB2. The 1918 PB2 protein was found to be both necessary and sufficient for airborne transmission of a virus expressing the 1918 HA and neuraminidase. Also, it was found that influenza viruses that were able to transmit efficiently in ferrets were able to replicate efficiently at the lower temperature (33 °C) found in the environment of mammalian airway. These findings demonstrate that the adaptation of the HA and PB2 proteins are critical for the development of pandemic influenza strains from avian influenza viruses.
Url:
DOI: 10.1073/pnas.0813172106
PubMed: 19211790
PubMed Central: 2651239
Affiliations:
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PMC:2651239Le document en format XML
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Tumpey</name><affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Mailstop G-16, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Atlanta, GA 30333;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Influenza Division, Mailstop G-16, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Atlanta</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">19211790</idno><idno type="pmc">2651239</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2651239</idno><idno type="RBID">PMC:2651239</idno><idno type="doi">10.1073/pnas.0813172106</idno><date when="2009">2009</date><idno type="wicri:Area/Pmc/Corpus">000770</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000770</idno><idno type="wicri:Area/Pmc/Curation">000770</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000770</idno><idno type="wicri:Area/Pmc/Checkpoint">000C00</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000C00</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Human HA and polymerase subunit PB2 proteins confer transmission of an avian influenza virus through the air</title><author><name sortKey="Van Hoeven, Neal" sort="Van Hoeven, Neal" uniqKey="Van Hoeven N" first="Neal" last="Van Hoeven">Neal Van Hoeven</name><affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Mailstop G-16, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Atlanta, GA 30333;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Influenza Division, Mailstop G-16, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Atlanta</wicri:cityArea></affiliation></author><author><name sortKey="Pappas, Claudia" sort="Pappas, Claudia" uniqKey="Pappas C" first="Claudia" last="Pappas">Claudia Pappas</name><affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Mailstop G-16, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Atlanta, GA 30333;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Influenza Division, Mailstop G-16, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Atlanta</wicri:cityArea></affiliation></author><author><name sortKey="Belser, Jessica A" sort="Belser, Jessica A" uniqKey="Belser J" first="Jessica A." last="Belser">Jessica A. Belser</name><affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Mailstop G-16, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Atlanta, GA 30333;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Influenza Division, Mailstop G-16, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Atlanta</wicri:cityArea></affiliation><affiliation><nlm:aff id="aff2">Department of Microbiology,</nlm:aff></affiliation></author><author><name sortKey="Maines, Taronna R" sort="Maines, Taronna R" uniqKey="Maines T" first="Taronna R." last="Maines">Taronna R. Maines</name><affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Mailstop G-16, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Atlanta, GA 30333;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Influenza Division, Mailstop G-16, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Atlanta</wicri:cityArea></affiliation></author><author><name sortKey="Zeng, Hui" sort="Zeng, Hui" uniqKey="Zeng H" first="Hui" last="Zeng">Hui Zeng</name><affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Mailstop G-16, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Atlanta, GA 30333;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Influenza Division, Mailstop G-16, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Atlanta</wicri:cityArea></affiliation></author><author><name sortKey="Garcia Sastre, Adolfo" sort="Garcia Sastre, Adolfo" uniqKey="Garcia Sastre A" first="Adolfo" last="García-Sastre">Adolfo García-Sastre</name><affiliation><nlm:aff id="aff2">Department of Microbiology,</nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff3">Department of Medicine, Division of Infectious Diseases</nlm:aff><wicri:noCountry code="subfield">Division of Infectious Diseases</wicri:noCountry></affiliation><affiliation wicri:level="2"><nlm:aff wicri:cut="; and" id="aff4">Emerging Pathogens Institute, Mount Sinai School of Medicine, New York, NY 10029</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Emerging Pathogens Institute, Mount Sinai School of Medicine, New York</wicri:cityArea></affiliation></author><author><name sortKey="Sasisekharan, Ram" sort="Sasisekharan, Ram" uniqKey="Sasisekharan R" first="Ram" last="Sasisekharan">Ram Sasisekharan</name><affiliation><nlm:aff wicri:cut=" and" id="aff5">Department of Biological Engineering</nlm:aff></affiliation><affiliation wicri:level="2"><nlm:aff id="aff6">Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Massachusetts</region></placeName><wicri:cityArea>Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge</wicri:cityArea></affiliation></author><author><name sortKey="Katz, Jacqueline M" sort="Katz, Jacqueline M" uniqKey="Katz J" first="Jacqueline M." last="Katz">Jacqueline M. Katz</name><affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Mailstop G-16, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Atlanta, GA 30333;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Influenza Division, Mailstop G-16, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Atlanta</wicri:cityArea></affiliation></author><author><name sortKey="Tumpey, Terrence M" sort="Tumpey, Terrence M" uniqKey="Tumpey T" first="Terrence M." last="Tumpey">Terrence M. Tumpey</name><affiliation wicri:level="2"><nlm:aff id="aff1">Influenza Division, Mailstop G-16, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Atlanta, GA 30333;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Influenza Division, Mailstop G-16, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Atlanta</wicri:cityArea></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><idno type="eISSN">1091-6490</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The influenza virus genes that confer efficient transmission of epidemic and pandemic strains in humans have not been identified. The rapid spread and severe disease caused by the 1918 influenza pandemic virus makes it an ideal virus to study the transmissibility of potentially pandemic influenza strains. Here, we used a series of human 1918-avian H1N1 influenza reassortant viruses to identify the genetic determinants that govern airborne transmission of avian influenza viruses. We have demonstrated that the 1918 HA gene was necessary for efficient direct contact transmission, but did not allow respiratory droplet transmission between ferrets of an avian influenza virus possessing an avian polymerase subunit PB2. The 1918 PB2 protein was found to be both necessary and sufficient for airborne transmission of a virus expressing the 1918 HA and neuraminidase. Also, it was found that influenza viruses that were able to transmit efficiently in ferrets were able to replicate efficiently at the lower temperature (33 °C) found in the environment of mammalian airway. These findings demonstrate that the adaptation of the HA and PB2 proteins are critical for the development of pandemic influenza strains from avian influenza viruses.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Proc Natl Acad Sci U S A</journal-id><journal-id journal-id-type="hwp">pnas</journal-id><journal-id journal-id-type="pmc">pnas</journal-id><journal-id journal-id-type="publisher-id">PNAS</journal-id><journal-title-group><journal-title>Proceedings of the National Academy of Sciences of the United States of America</journal-title></journal-title-group><issn pub-type="ppub">0027-8424</issn><issn pub-type="epub">1091-6490</issn><publisher><publisher-name>National Academy of Sciences</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">19211790</article-id><article-id pub-id-type="pmc">2651239</article-id><article-id pub-id-type="publisher-id">6770</article-id><article-id pub-id-type="doi">10.1073/pnas.0813172106</article-id><article-categories><subj-group subj-group-type="heading"><subject>Biological Sciences</subject><subj-group><subject>Medical Sciences</subject></subj-group></subj-group></article-categories><title-group><article-title>Human HA and polymerase subunit PB2 proteins confer transmission of an avian influenza virus through the air</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Van Hoeven</surname><given-names>Neal</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Pappas</surname><given-names>Claudia</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Belser</surname><given-names>Jessica A.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Maines</surname><given-names>Taronna R.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zeng</surname><given-names>Hui</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>García-Sastre</surname><given-names>Adolfo</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref><xref ref-type="aff" rid="aff3"><sup>c</sup></xref><xref ref-type="aff" rid="aff4"><sup>d</sup></xref></contrib><contrib contrib-type="author"><name><surname>Sasisekharan</surname><given-names>Ram</given-names></name><xref ref-type="aff" rid="aff5"><sup>e</sup></xref><xref ref-type="aff" rid="aff6"><sup>f</sup></xref></contrib><contrib contrib-type="author"><name><surname>Katz</surname><given-names>Jacqueline M.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Tumpey</surname><given-names>Terrence M.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref><xref ref-type="corresp" rid="cor1"><sup>1</sup></xref></contrib><aff id="aff1"><sup>a</sup>Influenza Division, Mailstop G-16, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road Northeast, Atlanta, GA 30333;</aff><aff id="aff2"><sup>b</sup>Department of Microbiology,</aff><aff id="aff3"><sup>c</sup>Department of Medicine, Division of Infectious Diseases, and</aff><aff id="aff4"><sup>d</sup>Emerging Pathogens Institute, Mount Sinai School of Medicine, New York, NY 10029; and</aff><aff id="aff5"><sup>e</sup>Department of Biological Engineering and</aff><aff id="aff6"><sup>f</sup>Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139</aff></contrib-group><author-notes><corresp id="cor1"><sup>1</sup>To whom correspondence should be addressed. E-mail: <email>tft9@cdc.gov</email></corresp><fn fn-type="com"><p>Communicated by Robert Langer, Massachusetts Institute of Technology, Cambridge, MA, December 26, 2008</p></fn><fn fn-type="con"><p>Author contributions: N.V.H., J.M.K., and T.M.T. designed research; N.V.H., C.P., J.A.B., T.R.M., H.Z., and T.M.T. performed research; A.G.-S. and R.S. contributed new reagents/analytic tools; N.V.H., R.S., J.M.K., and T.M.T. analyzed data; and N.V.H., J.M.K., and T.M.T. wrote the paper.</p></fn><fn fn-type="conflict"><p>The authors declare no conflict of interest.</p></fn></author-notes><pub-date pub-type="ppub"><day>3</day><month>3</month><year>2009</year></pub-date><pub-date pub-type="epub"><day>11</day><month>2</month><year>2009</year></pub-date><volume>106</volume><issue>9</issue><fpage>3366</fpage><lpage>3371</lpage><history><date date-type="received"><day>1</day><month>12</month><year>2008</year></date></history><permissions></permissions><self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zpq00909003366.pdf"></self-uri><abstract><p>The influenza virus genes that confer efficient transmission of epidemic and pandemic strains in humans have not been identified. The rapid spread and severe disease caused by the 1918 influenza pandemic virus makes it an ideal virus to study the transmissibility of potentially pandemic influenza strains. Here, we used a series of human 1918-avian H1N1 influenza reassortant viruses to identify the genetic determinants that govern airborne transmission of avian influenza viruses. We have demonstrated that the 1918 HA gene was necessary for efficient direct contact transmission, but did not allow respiratory droplet transmission between ferrets of an avian influenza virus possessing an avian polymerase subunit PB2. The 1918 PB2 protein was found to be both necessary and sufficient for airborne transmission of a virus expressing the 1918 HA and neuraminidase. Also, it was found that influenza viruses that were able to transmit efficiently in ferrets were able to replicate efficiently at the lower temperature (33 °C) found in the environment of mammalian airway. These findings demonstrate that the adaptation of the HA and PB2 proteins are critical for the development of pandemic influenza strains from avian influenza viruses.</p></abstract><kwd-group><kwd>ferrets</kwd><kwd>1918 pandemic</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>États-Unis</li></country><region><li>Géorgie (États-Unis)</li><li>Massachusetts</li><li>État de New York</li></region></list><tree><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Van Hoeven, Neal" sort="Van Hoeven, Neal" uniqKey="Van Hoeven N" first="Neal" last="Van Hoeven">Neal Van Hoeven</name></region><name sortKey="Belser, Jessica A" sort="Belser, Jessica A" uniqKey="Belser J" first="Jessica A." last="Belser">Jessica A. Belser</name><name sortKey="Garcia Sastre, Adolfo" sort="Garcia Sastre, Adolfo" uniqKey="Garcia Sastre A" first="Adolfo" last="García-Sastre">Adolfo García-Sastre</name><name sortKey="Katz, Jacqueline M" sort="Katz, Jacqueline M" uniqKey="Katz J" first="Jacqueline M." last="Katz">Jacqueline M. Katz</name><name sortKey="Maines, Taronna R" sort="Maines, Taronna R" uniqKey="Maines T" first="Taronna R." last="Maines">Taronna R. Maines</name><name sortKey="Pappas, Claudia" sort="Pappas, Claudia" uniqKey="Pappas C" first="Claudia" last="Pappas">Claudia Pappas</name><name sortKey="Sasisekharan, Ram" sort="Sasisekharan, Ram" uniqKey="Sasisekharan R" first="Ram" last="Sasisekharan">Ram Sasisekharan</name><name sortKey="Tumpey, Terrence M" sort="Tumpey, Terrence M" uniqKey="Tumpey T" first="Terrence M." last="Tumpey">Terrence M. Tumpey</name><name sortKey="Zeng, Hui" sort="Zeng, Hui" uniqKey="Zeng H" first="Hui" last="Zeng">Hui Zeng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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