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Comparison of Molecular Assays for the Rapid Detection and Simultaneous Subtype Differentiation of the Pandemic Influenza A (H1N1) 2009 Virus

Identifieur interne : 001901 ( PascalFrancis/Curation ); précédent : 001900; suivant : 001902

Comparison of Molecular Assays for the Rapid Detection and Simultaneous Subtype Differentiation of the Pandemic Influenza A (H1N1) 2009 Virus

Auteurs : LEEMI KYUNG [Corée du Sud] ; HYE RYOUN KIM [Corée du Sud]

Source :

RBID : Pascal:12-0365299

Descripteurs français

English descriptors

Abstract

In April 2009, the H1N1 pandemic influenza virus emerged as a novel influenza virus. The aim of this study was to compare the performances of several molecular assays, including conventional reverse transcription polymerase chain reaction (RT-PCR), two real-time reverse transcription (rRT)-PCRs, and two multiplex RT-PCRs. A total of 381 clinical specimens were collected from patients (223 men and 158 women), and both the Seeplex RV7 assay and rRT-PCR were ordered on different specimens within one week after collection. The concordance rate for the two methods was 87% (332/381), and the discrepancy rate was 13% (49/381). The positive rates for the molecular assays studied included 93.1% for the multiplex Seeplex RV7 assay, 93.1% for conventional reverse transcription (cRT)-PCR, 89.7% for the multiplex Seeplex Flu ACE Subtyping assay, 82.8% for protocol B rRT-PCR, and 58.6% for protocol A rRT-PCR. Our results showed that the multiplex Seeplex assays and the cRT-PCR yielded higher detection rates than rRT-PCRs for detecting the influenza A (H1N1) virus. Although the multiplex Seeplex assays had the advantage of simultaneous detection of several viruses, they were time-consuming and troublesome. Our results show that, although rRT-PCR had the advantage, the detection rates of the molecular assays varied depending upon the source of the influenza A (H1N1)v virus. Our findings also suggest that rRT-PCR sometimes detected virus in extremely low abundance and thus required validation of analytical performance and clinical correlation.
pA  
A01 01  1    @0 1017-7825
A03   1    @0 J. microbiol. biotechnol.
A05       @2 22
A06       @2 8
A08 01  1  ENG  @1 Comparison of Molecular Assays for the Rapid Detection and Simultaneous Subtype Differentiation of the Pandemic Influenza A (H1N1) 2009 Virus
A11 01  1    @1 LEEMI KYUNG
A11 02  1    @1 HYE RYOUN KIM
A14 01      @1 Department of Laboratory Medicine, Chung-Ang University College of Medicine @2 Seoul 156-755 @3 KOR @Z 1 aut. @Z 2 aut.
A20       @1 1165-1169
A21       @1 2012
A23 01      @0 ENG
A43 01      @1 INIST @2 26482 @5 354000508138240190
A44       @0 0000 @1 © 2012 INIST-CNRS. All rights reserved.
A45       @0 20 ref.
A47 01  1    @0 12-0365299
A60       @1 P
A61       @0 A
A64 01  1    @0 Journal of microbiology and biotechnology
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C01 01    ENG  @0 In April 2009, the H1N1 pandemic influenza virus emerged as a novel influenza virus. The aim of this study was to compare the performances of several molecular assays, including conventional reverse transcription polymerase chain reaction (RT-PCR), two real-time reverse transcription (rRT)-PCRs, and two multiplex RT-PCRs. A total of 381 clinical specimens were collected from patients (223 men and 158 women), and both the Seeplex RV7 assay and rRT-PCR were ordered on different specimens within one week after collection. The concordance rate for the two methods was 87% (332/381), and the discrepancy rate was 13% (49/381). The positive rates for the molecular assays studied included 93.1% for the multiplex Seeplex RV7 assay, 93.1% for conventional reverse transcription (cRT)-PCR, 89.7% for the multiplex Seeplex Flu ACE Subtyping assay, 82.8% for protocol B rRT-PCR, and 58.6% for protocol A rRT-PCR. Our results showed that the multiplex Seeplex assays and the cRT-PCR yielded higher detection rates than rRT-PCRs for detecting the influenza A (H1N1) virus. Although the multiplex Seeplex assays had the advantage of simultaneous detection of several viruses, they were time-consuming and troublesome. Our results show that, although rRT-PCR had the advantage, the detection rates of the molecular assays varied depending upon the source of the influenza A (H1N1)v virus. Our findings also suggest that rRT-PCR sometimes detected virus in extremely low abundance and thus required validation of analytical performance and clinical correlation.
C02 01  X    @0 002A31
C02 02  X    @0 215
C03 01  X  FRE  @0 Etude comparative @5 01
C03 01  X  ENG  @0 Comparative study @5 01
C03 01  X  SPA  @0 Estudio comparativo @5 01
C03 02  X  FRE  @0 Technique rapide @5 02
C03 02  X  ENG  @0 Rapid technique @5 02
C03 02  X  SPA  @0 Técnica rápida @5 02
C03 03  X  FRE  @0 Détection @5 10
C03 03  X  ENG  @0 Detection @5 10
C03 03  X  SPA  @0 Detección @5 10
C03 04  X  FRE  @0 Soustype @5 11
C03 04  X  ENG  @0 Subtype @5 11
C03 04  X  SPA  @0 Subtipo @5 11
C03 05  X  FRE  @0 Différenciation @5 12
C03 05  X  ENG  @0 Differentiation @5 12
C03 05  X  SPA  @0 Diferenciación @5 12
C03 06  X  FRE  @0 Virus @2 NW @5 13
C03 06  X  ENG  @0 Virus @2 NW @5 13
C03 06  X  SPA  @0 Virus @2 NW @5 13
C03 07  X  FRE  @0 Influenzavirus @2 NW @5 14
C03 07  X  ENG  @0 Influenzavirus @2 NW @5 14
C03 07  X  SPA  @0 Influenzavirus @2 NW @5 14
C03 08  X  FRE  @0 Grippe A @5 19
C03 08  X  ENG  @0 Influenza A @5 19
C03 08  X  SPA  @0 Gripe A @5 19
C03 09  X  FRE  @0 Temps réel @5 21
C03 09  X  ENG  @0 Real time @5 21
C03 09  X  SPA  @0 Tiempo real @5 21
C03 10  X  FRE  @0 Réaction chaîne polymérase @5 22
C03 10  X  ENG  @0 Polymerase chain reaction @5 22
C03 10  X  SPA  @0 Reacción cadena polimerasa @5 22
C03 11  X  FRE  @0 Réaction chaîne polymérase RT @5 24
C03 11  X  ENG  @0 Reverse transcription polymerase chain reaction @5 24
C03 11  X  SPA  @0 Reacción cadena polimerasa transcripción inversa @5 24
C03 12  X  FRE  @0 Grippe H1N1 @4 CD @5 96
C03 12  X  ENG  @0 H1N1 influenza @4 CD @5 96
C03 12  X  SPA  @0 Gripe H1N1 @4 CD @5 96
C07 01  X  FRE  @0 Orthomyxoviridae @2 NW
C07 01  X  ENG  @0 Orthomyxoviridae @2 NW
C07 01  X  SPA  @0 Orthomyxoviridae @2 NW
C07 02  X  FRE  @0 Virose
C07 02  X  ENG  @0 Viral disease
C07 02  X  SPA  @0 Virosis
C07 03  X  FRE  @0 Infection
C07 03  X  ENG  @0 Infection
C07 03  X  SPA  @0 Infección
N21       @1 282
N44 01      @1 OTO
N82       @1 OTO

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Pascal:12-0365299

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<div type="abstract" xml:lang="en">In April 2009, the H1N1 pandemic influenza virus emerged as a novel influenza virus. The aim of this study was to compare the performances of several molecular assays, including conventional reverse transcription polymerase chain reaction (RT-PCR), two real-time reverse transcription (rRT)-PCRs, and two multiplex RT-PCRs. A total of 381 clinical specimens were collected from patients (223 men and 158 women), and both the Seeplex RV7 assay and rRT-PCR were ordered on different specimens within one week after collection. The concordance rate for the two methods was 87% (332/381), and the discrepancy rate was 13% (49/381). The positive rates for the molecular assays studied included 93.1% for the multiplex Seeplex RV7 assay, 93.1% for conventional reverse transcription (cRT)-PCR, 89.7% for the multiplex Seeplex Flu ACE Subtyping assay, 82.8% for protocol B rRT-PCR, and 58.6% for protocol A rRT-PCR. Our results showed that the multiplex Seeplex assays and the cRT-PCR yielded higher detection rates than rRT-PCRs for detecting the influenza A (H1N1) virus. Although the multiplex Seeplex assays had the advantage of simultaneous detection of several viruses, they were time-consuming and troublesome. Our results show that, although rRT-PCR had the advantage, the detection rates of the molecular assays varied depending upon the source of the influenza A (H1N1)v virus. Our findings also suggest that rRT-PCR sometimes detected virus in extremely low abundance and thus required validation of analytical performance and clinical correlation.</div>
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