Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection
Identifieur interne : 001972 ( PascalFrancis/Corpus ); précédent : 001971; suivant : 001973Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection
Auteurs : John A. Rutigliano ; Peter C. Doherty ; John Franks ; Melissa Y. Morris ; Cory Reynolds ; Paul G. ThomasSource :
- Journal of immunological methods [ 0022-1759 ] ; 2008.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Influenza virus infections carry a high public health cost, and pandemics are potentially catastrophic. Though the ferret is generally regarded as the best model for human influenza, few reagents are available for the analysis of cellular immunity. We thus screened monoclonal antibodies (mAbs) made for identifying immune cells in other species to see if any were cross-reactive. Flow cytometric analysis of lymphocytes isolated from blood, spleen, and lung of normal and virus-infected ferrets indicated that several mouse mAbs bound to the corresponding antigens in ferrets. Typing bronchoalveolar lavage populations from pneumonic ferrets with mAb to human CD8 showed the massive CD8+ T cell enrichment characteristic of this infection in mice. The availability of this, and several other mAbs that showed cross-reactivity, should allow us to begin the dissection of cell-mediated immunity in the ferret, which, at least from these early results, looks similar to the situation in mice.
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Pour connaître la documentation sur le format Inist Standard.
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Format Inist (serveur)
NO : | PASCAL 08-0315453 INIST |
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ET : | Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection |
AU : | RUTIGLIANO (John A.); DOHERTY (Peter C.); FRANKS (John); MORRIS (Melissa Y.); REYNOLDS (Cory); THOMAS (Paul G.) |
AF : | Department of Immunology, St. Jude Children's Research Hospital/Memphis, TN, 38105/Etats-Unis (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut.); Department of Microbiology and Immunology, University of Melbourne/Vic 3010/Australie (2 aut.) |
DT : | Publication en série; Papier de recherche; Niveau analytique |
SO : | Journal of immunological methods; ISSN 0022-1759; Coden JIMMBG; Pays-Bas; Da. 2008; Vol. 336; No. 1; Pp. 71-77; Bibl. 1/2 p. |
LA : | Anglais |
EA : | Influenza virus infections carry a high public health cost, and pandemics are potentially catastrophic. Though the ferret is generally regarded as the best model for human influenza, few reagents are available for the analysis of cellular immunity. We thus screened monoclonal antibodies (mAbs) made for identifying immune cells in other species to see if any were cross-reactive. Flow cytometric analysis of lymphocytes isolated from blood, spleen, and lung of normal and virus-infected ferrets indicated that several mouse mAbs bound to the corresponding antigens in ferrets. Typing bronchoalveolar lavage populations from pneumonic ferrets with mAb to human CD8 showed the massive CD8+ T cell enrichment characteristic of this infection in mice. The availability of this, and several other mAbs that showed cross-reactivity, should allow us to begin the dissection of cell-mediated immunity in the ferret, which, at least from these early results, looks similar to the situation in mice. |
CC : | 002A06B06 |
FD : | Souris; Anticorps monoclonal; Réaction croisée; Modèle; Lymphocyte T; Cytométrie flux; Méthode immunologique; Grippe |
FG : | Rodentia; Mammalia; Vertebrata; Virose; Infection |
ED : | Mouse; Monoclonal antibody; Cross reaction; Models; T-Lymphocyte; Flow cytometry; Immunological method; Influenza |
EG : | Rodentia; Mammalia; Vertebrata; Viral disease; Infection |
SD : | Ratón; Anticuerpo monoclonal; Reacción cruzada; Modelo; Linfocito T; Citometría flujo; Método inmunológico; Gripe |
LO : | INIST-15654.354000197948340100 |
ID : | 08-0315453 |
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Pascal:08-0315453Le document en format XML
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<front><div type="abstract" xml:lang="en">Influenza virus infections carry a high public health cost, and pandemics are potentially catastrophic. Though the ferret is generally regarded as the best model for human influenza, few reagents are available for the analysis of cellular immunity. We thus screened monoclonal antibodies (mAbs) made for identifying immune cells in other species to see if any were cross-reactive. Flow cytometric analysis of lymphocytes isolated from blood, spleen, and lung of normal and virus-infected ferrets indicated that several mouse mAbs bound to the corresponding antigens in ferrets. Typing bronchoalveolar lavage populations from pneumonic ferrets with mAb to human CD8 showed the massive CD8+ T cell enrichment characteristic of this infection in mice. The availability of this, and several other mAbs that showed cross-reactivity, should allow us to begin the dissection of cell-mediated immunity in the ferret, which, at least from these early results, looks similar to the situation in mice.</div>
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<server><NO>PASCAL 08-0315453 INIST</NO>
<ET>Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection</ET>
<AU>RUTIGLIANO (John A.); DOHERTY (Peter C.); FRANKS (John); MORRIS (Melissa Y.); REYNOLDS (Cory); THOMAS (Paul G.)</AU>
<AF>Department of Immunology, St. Jude Children's Research Hospital/Memphis, TN, 38105/Etats-Unis (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut.); Department of Microbiology and Immunology, University of Melbourne/Vic 3010/Australie (2 aut.)</AF>
<DT>Publication en série; Papier de recherche; Niveau analytique</DT>
<SO>Journal of immunological methods; ISSN 0022-1759; Coden JIMMBG; Pays-Bas; Da. 2008; Vol. 336; No. 1; Pp. 71-77; Bibl. 1/2 p.</SO>
<LA>Anglais</LA>
<EA>Influenza virus infections carry a high public health cost, and pandemics are potentially catastrophic. Though the ferret is generally regarded as the best model for human influenza, few reagents are available for the analysis of cellular immunity. We thus screened monoclonal antibodies (mAbs) made for identifying immune cells in other species to see if any were cross-reactive. Flow cytometric analysis of lymphocytes isolated from blood, spleen, and lung of normal and virus-infected ferrets indicated that several mouse mAbs bound to the corresponding antigens in ferrets. Typing bronchoalveolar lavage populations from pneumonic ferrets with mAb to human CD8 showed the massive CD8+ T cell enrichment characteristic of this infection in mice. The availability of this, and several other mAbs that showed cross-reactivity, should allow us to begin the dissection of cell-mediated immunity in the ferret, which, at least from these early results, looks similar to the situation in mice.</EA>
<CC>002A06B06</CC>
<FD>Souris; Anticorps monoclonal; Réaction croisée; Modèle; Lymphocyte T; Cytométrie flux; Méthode immunologique; Grippe</FD>
<FG>Rodentia; Mammalia; Vertebrata; Virose; Infection</FG>
<ED>Mouse; Monoclonal antibody; Cross reaction; Models; T-Lymphocyte; Flow cytometry; Immunological method; Influenza</ED>
<EG>Rodentia; Mammalia; Vertebrata; Viral disease; Infection</EG>
<SD>Ratón; Anticuerpo monoclonal; Reacción cruzada; Modelo; Linfocito T; Citometría flujo; Método inmunológico; Gripe</SD>
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<ID>08-0315453</ID>
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