Detection of swine-origin influenza A (H1N1) viruses using a localized surface plasmon coupled fluorescence fiber-optic biosensor
Identifieur interne : 001290 ( PascalFrancis/Checkpoint ); précédent : 001289; suivant : 001291Detection of swine-origin influenza A (H1N1) viruses using a localized surface plasmon coupled fluorescence fiber-optic biosensor
Auteurs : Ying-Feng Chang [Taïwan] ; Sheng-Fan Wang [Taïwan] ; Jason C. Huang [Taïwan] ; Li-Chen Su [Taïwan] ; LING YAO [Taïwan] ; Ying-Chang Li [Taïwan] ; Suh-Chin Wu [Taïwan] ; Yi-Ming A. Chen [Taïwan] ; Jo-Ping Hsieh [Taïwan] ; CHIEN CHOU [Taïwan]Source :
- Biosensors & bioelectronics [ 0956-5663 ] ; 2010.
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Abstract
Swine-origin influenza A (H1N1) virus (S-OIV) was identified as a new reassortant strain of influenza A virus in April 2009 and led to an influenza pandemic. Accurate and timely diagnoses are crucial for the control of influenza disease. We developed a localized surface plasmon coupled fluorescence fiber-optic biosensor (LSPCF-FOB) which combines a sandwich immunoassay with the LSP technique using antibodies against the hemagglutinin (HA) proteins of S-OIVs. The detection limit of the LSPCF-FOB for recombinant S-OIV H1 protein detection was estimated at 13.9 pg/mL, which is 103-fold better than that of conventional capture ELISA when using the same capture antibodies. For clinical S-OIV isolates measurement, meanwhile, the detection limit of the LSPCF-FOB platform was calculated to be 8.25 x 104 copies/mL, compared with 2.06 x 106 copies/mL using conventional capture ELISA. Further-more, in comparison with the influenza A/B rapid test, the detection limit of the LSPCF-FOB for S-OIV was almost 50-fold in PBS solution and 25-fold lower in mimic solution, which used nasal mucosa from healthy donors as the diluent. The findings of this study therefore indicate that the high detection sensitivity and specificity of the LSPCF-FOB make it a potentially effective diagnostic tool for clinical S-OIV infection and this technique has the potential to be applied to the development of other clinical microbe detection platforms.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Detection of swine-origin influenza A (H1N1) viruses using a localized surface plasmon coupled fluorescence fiber-optic biosensor</title><author><name sortKey="Chang, Ying Feng" sort="Chang, Ying Feng" uniqKey="Chang Y" first="Ying-Feng" last="Chang">Ying-Feng Chang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute ofBiophotonics, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Graduate Institute of Electro-Optical Engineering, Chang Gung University</s1><s2>Taoyuan 333</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taoyuan 333</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Sheng Fan" sort="Wang, Sheng Fan" uniqKey="Wang S" first="Sheng-Fan" last="Wang">Sheng-Fan Wang</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>AIDS Prevention and Research Center, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Jason C" sort="Huang, Jason C" uniqKey="Huang J" first="Jason C." last="Huang">Jason C. Huang</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>AIDS Prevention and Research Center, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Education and Research, Taipei City Hospital</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>3 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Su, Li Chen" sort="Su, Li Chen" uniqKey="Su L" first="Li-Chen" last="Su">Li-Chen Su</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Graduate Institute of Electro-Optical Engineering, Chang Gung University</s1><s2>Taoyuan 333</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taoyuan 333</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Optics and Photonics, National Central University</s1><s2>Taoyuan 320</s2><s3>TWN</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taoyuan 320</wicri:noRegion></affiliation></author><author><name sortKey="Ling Yao" sort="Ling Yao" uniqKey="Ling Yao" last="Ling Yao">LING YAO</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Li, Ying Chang" sort="Li, Ying Chang" uniqKey="Li Y" first="Ying-Chang" last="Li">Ying-Chang Li</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Graduate Institute of Electro-Optical Engineering, Chang Gung University</s1><s2>Taoyuan 333</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taoyuan 333</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Optics and Photonics, National Central University</s1><s2>Taoyuan 320</s2><s3>TWN</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taoyuan 320</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Suh Chin" sort="Wu, Suh Chin" uniqKey="Wu S" first="Suh-Chin" last="Wu">Suh-Chin Wu</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Institute of Biotechnology, National Tsing-Hua University</s1><s2>Hsinchu 300</s2><s3>TWN</s3><sZ>7 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Hsinchu 300</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Yi Ming A" sort="Chen, Yi Ming A" uniqKey="Chen Y" first="Yi-Ming A." last="Chen">Yi-Ming A. Chen</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>AIDS Prevention and Research Center, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Hsieh, Jo Ping" sort="Hsieh, Jo Ping" uniqKey="Hsieh J" first="Jo-Ping" last="Hsieh">Jo-Ping Hsieh</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute ofBiophotonics, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Graduate Institute of Electro-Optical Engineering, Chang Gung University</s1><s2>Taoyuan 333</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taoyuan 333</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>Chang Gung Molecular Medicine Research Center, Chang Gung University</s1><s2>Taoyuan 333</s2><s3>TWN</s3><sZ>9 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taoyuan 333</wicri:noRegion></affiliation></author><author><name sortKey="Chien Chou" sort="Chien Chou" uniqKey="Chien Chou" last="Chien Chou">CHIEN CHOU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute ofBiophotonics, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Graduate Institute of Electro-Optical Engineering, Chang Gung University</s1><s2>Taoyuan 333</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taoyuan 333</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Optics and Photonics, National Central University</s1><s2>Taoyuan 320</s2><s3>TWN</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taoyuan 320</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">11-0117774</idno><date when="2010">2010</date><idno type="stanalyst">PASCAL 11-0117774 INIST</idno><idno type="RBID">Pascal:11-0117774</idno><idno type="wicri:Area/PascalFrancis/Corpus">000E48</idno><idno type="wicri:Area/PascalFrancis/Curation">001005</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">001290</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">001290</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Detection of swine-origin influenza A (H1N1) viruses using a localized surface plasmon coupled fluorescence fiber-optic biosensor</title><author><name sortKey="Chang, Ying Feng" sort="Chang, Ying Feng" uniqKey="Chang Y" first="Ying-Feng" last="Chang">Ying-Feng Chang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute ofBiophotonics, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Graduate Institute of Electro-Optical Engineering, Chang Gung University</s1><s2>Taoyuan 333</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taoyuan 333</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Sheng Fan" sort="Wang, Sheng Fan" uniqKey="Wang S" first="Sheng-Fan" last="Wang">Sheng-Fan Wang</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>AIDS Prevention and Research Center, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Jason C" sort="Huang, Jason C" uniqKey="Huang J" first="Jason C." last="Huang">Jason C. Huang</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>AIDS Prevention and Research Center, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Education and Research, Taipei City Hospital</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>3 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Su, Li Chen" sort="Su, Li Chen" uniqKey="Su L" first="Li-Chen" last="Su">Li-Chen Su</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Graduate Institute of Electro-Optical Engineering, Chang Gung University</s1><s2>Taoyuan 333</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taoyuan 333</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Optics and Photonics, National Central University</s1><s2>Taoyuan 320</s2><s3>TWN</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taoyuan 320</wicri:noRegion></affiliation></author><author><name sortKey="Ling Yao" sort="Ling Yao" uniqKey="Ling Yao" last="Ling Yao">LING YAO</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Li, Ying Chang" sort="Li, Ying Chang" uniqKey="Li Y" first="Ying-Chang" last="Li">Ying-Chang Li</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Graduate Institute of Electro-Optical Engineering, Chang Gung University</s1><s2>Taoyuan 333</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taoyuan 333</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Optics and Photonics, National Central University</s1><s2>Taoyuan 320</s2><s3>TWN</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taoyuan 320</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Suh Chin" sort="Wu, Suh Chin" uniqKey="Wu S" first="Suh-Chin" last="Wu">Suh-Chin Wu</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Institute of Biotechnology, National Tsing-Hua University</s1><s2>Hsinchu 300</s2><s3>TWN</s3><sZ>7 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Hsinchu 300</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Yi Ming A" sort="Chen, Yi Ming A" uniqKey="Chen Y" first="Yi-Ming A." last="Chen">Yi-Ming A. Chen</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>AIDS Prevention and Research Center, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Hsieh, Jo Ping" sort="Hsieh, Jo Ping" uniqKey="Hsieh J" first="Jo-Ping" last="Hsieh">Jo-Ping Hsieh</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute ofBiophotonics, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Graduate Institute of Electro-Optical Engineering, Chang Gung University</s1><s2>Taoyuan 333</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taoyuan 333</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>Chang Gung Molecular Medicine Research Center, Chang Gung University</s1><s2>Taoyuan 333</s2><s3>TWN</s3><sZ>9 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taoyuan 333</wicri:noRegion></affiliation></author><author><name sortKey="Chien Chou" sort="Chien Chou" uniqKey="Chien Chou" last="Chien Chou">CHIEN CHOU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute ofBiophotonics, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Graduate Institute of Electro-Optical Engineering, Chang Gung University</s1><s2>Taoyuan 333</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taoyuan 333</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Optics and Photonics, National Central University</s1><s2>Taoyuan 320</s2><s3>TWN</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taoyuan 320</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Biosensors & bioelectronics</title><title level="j" type="abbreviated">Biosens. bioelectron.</title><idno type="ISSN">0956-5663</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Biosensors & bioelectronics</title><title level="j" type="abbreviated">Biosens. bioelectron.</title><idno type="ISSN">0956-5663</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensor</term><term>Detection</term><term>Fluorescence</term><term>Gold</term><term>Influenza</term><term>Influenza A virus</term><term>Nanoparticle</term><term>Optical fiber</term><term>Pig</term><term>Surface plasmon</term><term>Swine</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Détection</term><term>Porc</term><term>Porcin</term><term>Grippe</term><term>Plasmon surface</term><term>Virus grippal A</term><term>Fluorescence</term><term>Fibre optique</term><term>Biodétecteur</term><term>Or</term><term>Nanoparticule</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Porcin</term><term>Or</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Swine-origin influenza A (H1N1) virus (S-OIV) was identified as a new reassortant strain of influenza A virus in April 2009 and led to an influenza pandemic. Accurate and timely diagnoses are crucial for the control of influenza disease. We developed a localized surface plasmon coupled fluorescence fiber-optic biosensor (LSPCF-FOB) which combines a sandwich immunoassay with the LSP technique using antibodies against the hemagglutinin (HA) proteins of S-OIVs. The detection limit of the LSPCF-FOB for recombinant S-OIV H1 protein detection was estimated at 13.9 pg/mL, which is 10<sup>3</sup>-fold better than that of conventional capture ELISA when using the same capture antibodies. For clinical S-OIV isolates measurement, meanwhile, the detection limit of the LSPCF-FOB platform was calculated to be 8.25 x 10<sup>4</sup> copies/mL, compared with 2.06 x 10<sup>6</sup> copies/mL using conventional capture ELISA. Further-more, in comparison with the influenza A/B rapid test, the detection limit of the LSPCF-FOB for S-OIV was almost 50-fold in PBS solution and 25-fold lower in mimic solution, which used nasal mucosa from healthy donors as the diluent. The findings of this study therefore indicate that the high detection sensitivity and specificity of the LSPCF-FOB make it a potentially effective diagnostic tool for clinical S-OIV infection and this technique has the potential to be applied to the development of other clinical microbe detection platforms.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0956-5663</s0></fA01><fA03 i2="1"><s0>Biosens. bioelectron.</s0></fA03><fA05><s2>26</s2></fA05><fA06><s2>3</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Detection of swine-origin influenza A (H1N1) viruses using a localized surface plasmon coupled fluorescence fiber-optic biosensor</s1></fA08><fA11 i1="01" i2="1"><s1>CHANG (Ying-Feng)</s1></fA11><fA11 i1="02" i2="1"><s1>WANG (Sheng-Fan)</s1></fA11><fA11 i1="03" i2="1"><s1>HUANG (Jason C.)</s1></fA11><fA11 i1="04" i2="1"><s1>SU (Li-Chen)</s1></fA11><fA11 i1="05" i2="1"><s1>LING YAO</s1></fA11><fA11 i1="06" i2="1"><s1>LI (Ying-Chang)</s1></fA11><fA11 i1="07" i2="1"><s1>WU (Suh-Chin)</s1></fA11><fA11 i1="08" i2="1"><s1>CHEN (Yi-Ming A.)</s1></fA11><fA11 i1="09" i2="1"><s1>HSIEH (Jo-Ping)</s1></fA11><fA11 i1="10" i2="1"><s1>CHIEN CHOU</s1></fA11><fA14 i1="01"><s1>Institute ofBiophotonics, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></fA14><fA14 i1="02"><s1>Graduate Institute of Electro-Optical Engineering, Chang Gung University</s1><s2>Taoyuan 333</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></fA14><fA14 i1="03"><s1>Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></fA14><fA14 i1="04"><s1>Institute of Biotechnology, National Tsing-Hua University</s1><s2>Hsinchu 300</s2><s3>TWN</s3><sZ>7 aut.</sZ></fA14><fA14 i1="05"><s1>AIDS Prevention and Research Center, National Yang-Ming University</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ></fA14><fA14 i1="06"><s1>Department of Education and Research, Taipei City Hospital</s1><s2>Taipei 112</s2><s3>TWN</s3><sZ>3 aut.</sZ></fA14><fA14 i1="07"><s1>Department of Optics and Photonics, National Central University</s1><s2>Taoyuan 320</s2><s3>TWN</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></fA14><fA14 i1="08"><s1>Chang Gung Molecular Medicine Research Center, Chang Gung University</s1><s2>Taoyuan 333</s2><s3>TWN</s3><sZ>9 aut.</sZ></fA14><fA20><s1>1068-1073</s1></fA20><fA21><s1>2010</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>20668</s2><s5>354000191936800180</s5></fA43><fA44><s0>0000</s0><s1>© 2011 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>1/4 p.</s0></fA45><fA47 i1="01" i2="1"><s0>11-0117774</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Biosensors & bioelectronics</s0></fA64><fA66 i1="01"><s0>GBR</s0></fA66><fC01 i1="01" l="ENG"><s0>Swine-origin influenza A (H1N1) virus (S-OIV) was identified as a new reassortant strain of influenza A virus in April 2009 and led to an influenza pandemic. Accurate and timely diagnoses are crucial for the control of influenza disease. We developed a localized surface plasmon coupled fluorescence fiber-optic biosensor (LSPCF-FOB) which combines a sandwich immunoassay with the LSP technique using antibodies against the hemagglutinin (HA) proteins of S-OIVs. The detection limit of the LSPCF-FOB for recombinant S-OIV H1 protein detection was estimated at 13.9 pg/mL, which is 10<sup>3</sup>-fold better than that of conventional capture ELISA when using the same capture antibodies. For clinical S-OIV isolates measurement, meanwhile, the detection limit of the LSPCF-FOB platform was calculated to be 8.25 x 10<sup>4</sup> copies/mL, compared with 2.06 x 10<sup>6</sup> copies/mL using conventional capture ELISA. Further-more, in comparison with the influenza A/B rapid test, the detection limit of the LSPCF-FOB for S-OIV was almost 50-fold in PBS solution and 25-fold lower in mimic solution, which used nasal mucosa from healthy donors as the diluent. The findings of this study therefore indicate that the high detection sensitivity and specificity of the LSPCF-FOB make it a potentially effective diagnostic tool for clinical S-OIV infection and this technique has the potential to be applied to the development of other clinical microbe detection platforms.</s0></fC01><fC02 i1="01" i2="X"><s0>002A31C09B</s0></fC02><fC02 i1="02" i2="X"><s0>215</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Détection</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Detection</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Detección</s0><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Porc</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Pig</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Cerdo</s0><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Porcin</s0><s5>10</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Swine</s0><s5>10</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Porcino</s0><s5>10</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Grippe</s0><s5>11</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Influenza</s0><s5>11</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Gripe</s0><s5>11</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Plasmon surface</s0><s5>12</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Surface plasmon</s0><s5>12</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Plasmón superficie</s0><s5>12</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Virus grippal A</s0><s2>NW</s2><s5>13</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Influenza A virus</s0><s2>NW</s2><s5>13</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Influenza A virus</s0><s2>NW</s2><s5>13</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Fluorescence</s0><s5>19</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Fluorescence</s0><s5>19</s5></fC03><fC03 i1="07" i2="X" l="SPA"><s0>Fluorescencia</s0><s5>19</s5></fC03><fC03 i1="08" i2="X" l="FRE"><s0>Fibre optique</s0><s5>20</s5></fC03><fC03 i1="08" i2="X" l="ENG"><s0>Optical fiber</s0><s5>20</s5></fC03><fC03 i1="08" i2="X" l="SPA"><s0>Fibra óptica</s0><s5>20</s5></fC03><fC03 i1="09" i2="X" l="FRE"><s0>Biodétecteur</s0><s5>21</s5></fC03><fC03 i1="09" i2="X" l="ENG"><s0>Biosensor</s0><s5>21</s5></fC03><fC03 i1="09" i2="X" l="SPA"><s0>Biodetector</s0><s5>21</s5></fC03><fC03 i1="10" i2="X" l="FRE"><s0>Or</s0><s2>NC</s2><s5>22</s5></fC03><fC03 i1="10" i2="X" l="ENG"><s0>Gold</s0><s2>NC</s2><s5>22</s5></fC03><fC03 i1="10" i2="X" l="SPA"><s0>Oro</s0><s2>NC</s2><s5>22</s5></fC03><fC03 i1="11" i2="X" l="FRE"><s0>Nanoparticule</s0><s5>35</s5></fC03><fC03 i1="11" i2="X" l="ENG"><s0>Nanoparticle</s0><s5>35</s5></fC03><fC03 i1="11" i2="X" l="SPA"><s0>Nanopartícula</s0><s5>35</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Artiodactyla</s0><s2>NS</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Artiodactyla</s0><s2>NS</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Artiodactyla</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Ungulata</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Ungulata</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Ungulata</s0><s2>NS</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Mammalia</s0><s2>NS</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Mammalia</s0><s2>NS</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Mammalia</s0><s2>NS</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Vertebrata</s0><s2>NS</s2></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Vertebrata</s0><s2>NS</s2></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Vertebrata</s0><s2>NS</s2></fC07><fC07 i1="05" i2="X" l="FRE"><s0>Virose</s0></fC07><fC07 i1="05" i2="X" l="ENG"><s0>Viral disease</s0></fC07><fC07 i1="05" i2="X" l="SPA"><s0>Virosis</s0></fC07><fC07 i1="06" i2="X" l="FRE"><s0>Infection</s0></fC07><fC07 i1="06" i2="X" l="ENG"><s0>Infection</s0></fC07><fC07 i1="06" i2="X" l="SPA"><s0>Infección</s0></fC07><fC07 i1="07" i2="X" l="FRE"><s0>Influenzavirus A</s0><s2>NW</s2></fC07><fC07 i1="07" i2="X" l="ENG"><s0>Influenzavirus A</s0><s2>NW</s2></fC07><fC07 i1="07" i2="X" l="SPA"><s0>Influenzavirus A</s0><s2>NW</s2></fC07><fC07 i1="08" i2="X" l="FRE"><s0>Orthomyxoviridae</s0><s2>NW</s2></fC07><fC07 i1="08" i2="X" l="ENG"><s0>Orthomyxoviridae</s0><s2>NW</s2></fC07><fC07 i1="08" i2="X" l="SPA"><s0>Orthomyxoviridae</s0><s2>NW</s2></fC07><fC07 i1="09" i2="X" l="FRE"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="09" i2="X" l="ENG"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="09" i2="X" l="SPA"><s0>Virus</s0><s2>NW</s2></fC07><fN21><s1>073</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist><affiliations><list><country><li>Taïwan</li></country></list><tree><country name="Taïwan"><noRegion><name sortKey="Chang, Ying Feng" sort="Chang, Ying Feng" uniqKey="Chang Y" first="Ying-Feng" last="Chang">Ying-Feng Chang</name></noRegion><name sortKey="Chang, Ying Feng" sort="Chang, Ying Feng" uniqKey="Chang Y" first="Ying-Feng" last="Chang">Ying-Feng Chang</name><name sortKey="Chen, Yi Ming A" sort="Chen, Yi Ming A" uniqKey="Chen Y" first="Yi-Ming A." last="Chen">Yi-Ming A. Chen</name><name sortKey="Chien Chou" sort="Chien Chou" uniqKey="Chien Chou" last="Chien Chou">CHIEN CHOU</name><name sortKey="Chien Chou" sort="Chien Chou" uniqKey="Chien Chou" last="Chien Chou">CHIEN CHOU</name><name sortKey="Chien Chou" sort="Chien Chou" uniqKey="Chien Chou" last="Chien Chou">CHIEN CHOU</name><name sortKey="Hsieh, Jo Ping" sort="Hsieh, Jo Ping" uniqKey="Hsieh J" first="Jo-Ping" last="Hsieh">Jo-Ping Hsieh</name><name sortKey="Hsieh, Jo Ping" sort="Hsieh, Jo Ping" uniqKey="Hsieh J" first="Jo-Ping" last="Hsieh">Jo-Ping Hsieh</name><name sortKey="Hsieh, Jo Ping" sort="Hsieh, Jo Ping" uniqKey="Hsieh J" first="Jo-Ping" last="Hsieh">Jo-Ping Hsieh</name><name sortKey="Huang, Jason C" sort="Huang, Jason C" uniqKey="Huang J" first="Jason C." last="Huang">Jason C. Huang</name><name sortKey="Huang, Jason C" sort="Huang, Jason C" uniqKey="Huang J" first="Jason C." last="Huang">Jason C. Huang</name><name sortKey="Huang, Jason C" sort="Huang, Jason C" uniqKey="Huang J" first="Jason C." last="Huang">Jason C. Huang</name><name sortKey="Li, Ying Chang" sort="Li, Ying Chang" uniqKey="Li Y" first="Ying-Chang" last="Li">Ying-Chang Li</name><name sortKey="Li, Ying Chang" sort="Li, Ying Chang" uniqKey="Li Y" first="Ying-Chang" last="Li">Ying-Chang Li</name><name sortKey="Ling Yao" sort="Ling Yao" uniqKey="Ling Yao" last="Ling Yao">LING YAO</name><name sortKey="Su, Li Chen" sort="Su, Li Chen" uniqKey="Su L" first="Li-Chen" last="Su">Li-Chen Su</name><name sortKey="Su, Li Chen" sort="Su, Li Chen" uniqKey="Su L" first="Li-Chen" last="Su">Li-Chen Su</name><name sortKey="Wang, Sheng Fan" sort="Wang, Sheng Fan" uniqKey="Wang S" first="Sheng-Fan" last="Wang">Sheng-Fan Wang</name><name sortKey="Wang, Sheng Fan" sort="Wang, Sheng Fan" uniqKey="Wang S" first="Sheng-Fan" last="Wang">Sheng-Fan Wang</name><name sortKey="Wu, Suh Chin" sort="Wu, Suh Chin" uniqKey="Wu S" first="Suh-Chin" last="Wu">Suh-Chin Wu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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