Detection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods
Identifieur interne : 000684 ( PascalFrancis/Checkpoint ); précédent : 000683; suivant : 000685Detection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods
Auteurs : John M. Nicholls [République populaire de Chine] ; Linda P. W. Wong [République populaire de Chine] ; Renee W. Y. Chan [République populaire de Chine] ; Leo L. M. Poon [République populaire de Chine] ; Leo K. Y. So [République populaire de Chine] ; Hui-Ling Yen [République populaire de Chine] ; Kevin Fung [République populaire de Chine] ; Sjouke Van Poucke [Belgique] ; J. S. Malik Peiris [République populaire de Chine]Source :
- Journal of virological methods [ 0166-0934 ] ; 2012.
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Abstract
Tissues infected with highly pathogenic avian influenza viruses such as H5N1 and H7N7 are normally required to be fixed in formalin or paraformaldehyde before examination in order to inactivate the virus. In this study commercially available monoclonal antibodies to the influenza nucleoprotein (NP) were evaluated in order to determine which antibodies would identify positive cells in tissues fixed in formalin or paraformaldehyde. An assessment of which antigen retrieval process would unmask antigens blocked by formalin fixation was also made. Of six commercially available monoclonal antibodies tested, only one (HB65, European Veterinary Laboratories) was able to identify all formalin fixed avian, swine and human influenza virus infected tissues, and this was after pronase induced epitope retrieval. This monoclonal antibody is recommended for routine diagnostic use for the detection of influenza A infected tissues that have been fixed in formalin or paraformaldehyde.
Affiliations:
- Belgique, République populaire de Chine
- Province de Flandre-Orientale, Région flamande
- Gand
- Université de Gand
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So</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, The University of Hong Kong, Pok Fu Lam</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Yen, Hui Ling" sort="Yen, Hui Ling" uniqKey="Yen H" first="Hui-Ling" last="Yen">Hui-Ling Yen</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre of Influenza Research, The University of Hong Kong</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>The HKU-Pasteur Research Centre</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>The HKU-Pasteur Research Centre</wicri:noRegion></affiliation></author><author><name sortKey="Fung, Kevin" sort="Fung, Kevin" uniqKey="Fung K" first="Kevin" last="Fung">Kevin Fung</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, The University of Hong Kong, Pok Fu Lam</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Van Poucke, Sjouke" sort="Van Poucke, Sjouke" uniqKey="Van Poucke S" first="Sjouke" last="Van Poucke">Sjouke Van Poucke</name><affiliation wicri:level="4"><inist:fA14 i1="04"><s1>Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University</s1><s2>Merelbeke</s2><s3>BEL</s3><sZ>8 aut.</sZ></inist:fA14><country>Belgique</country><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Malik Peiris, J S" sort="Malik Peiris, J S" uniqKey="Malik Peiris J" first="J. 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Nicholls</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, The University of Hong Kong, Pok Fu Lam</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre of Influenza Research, The University of Hong Kong</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Wong, Linda P W" sort="Wong, Linda P W" uniqKey="Wong L" first="Linda P. W." last="Wong">Linda P. W. Wong</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, The University of Hong Kong, Pok Fu Lam</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Chan, Renee W Y" sort="Chan, Renee W Y" uniqKey="Chan R" first="Renee W. Y." last="Chan">Renee W. Y. Chan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, The University of Hong Kong, Pok Fu Lam</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre of Influenza Research, The University of Hong Kong</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre of Influenza Research, The University of Hong Kong</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="So, Leo K Y" sort="So, Leo K Y" uniqKey="So L" first="Leo K. Y." last="So">Leo K. Y. So</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, The University of Hong Kong, Pok Fu Lam</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Yen, Hui Ling" sort="Yen, Hui Ling" uniqKey="Yen H" first="Hui-Ling" last="Yen">Hui-Ling Yen</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre of Influenza Research, The University of Hong Kong</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>The HKU-Pasteur Research Centre</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>The HKU-Pasteur Research Centre</wicri:noRegion></affiliation></author><author><name sortKey="Fung, Kevin" sort="Fung, Kevin" uniqKey="Fung K" first="Kevin" last="Fung">Kevin Fung</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, The University of Hong Kong, Pok Fu Lam</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation></author><author><name sortKey="Van Poucke, Sjouke" sort="Van Poucke, Sjouke" uniqKey="Van Poucke S" first="Sjouke" last="Van Poucke">Sjouke Van Poucke</name><affiliation wicri:level="4"><inist:fA14 i1="04"><s1>Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University</s1><s2>Merelbeke</s2><s3>BEL</s3><sZ>8 aut.</sZ></inist:fA14><country>Belgique</country><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Malik Peiris, J S" sort="Malik Peiris, J S" uniqKey="Malik Peiris J" first="J. S." last="Malik Peiris">J. S. Malik Peiris</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre of Influenza Research, The University of Hong Kong</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hong Kong SAR</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>The HKU-Pasteur Research Centre</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>6 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>The HKU-Pasteur Research Centre</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of virological methods</title><title level="j" type="abbreviated">J. virol. methods</title><idno type="ISSN">0166-0934</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virological methods</title><title level="j" type="abbreviated">J. virol. methods</title><idno type="ISSN">0166-0934</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Detection</term><term>Immunohistochemistry</term><term>Influenza</term><term>Influenzavirus</term><term>Method</term><term>Nucleoprotein</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Influenzavirus</term><term>Détection</term><term>Immunohistochimie</term><term>Méthode</term><term>Nucléoprotéine</term><term>Grippe</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Tissues infected with highly pathogenic avian influenza viruses such as H5N1 and H7N7 are normally required to be fixed in formalin or paraformaldehyde before examination in order to inactivate the virus. In this study commercially available monoclonal antibodies to the influenza nucleoprotein (NP) were evaluated in order to determine which antibodies would identify positive cells in tissues fixed in formalin or paraformaldehyde. An assessment of which antigen retrieval process would unmask antigens blocked by formalin fixation was also made. Of six commercially available monoclonal antibodies tested, only one (HB65, European Veterinary Laboratories) was able to identify all formalin fixed avian, swine and human influenza virus infected tissues, and this was after pronase induced epitope retrieval. This monoclonal antibody is recommended for routine diagnostic use for the detection of influenza A infected tissues that have been fixed in formalin or paraformaldehyde.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0166-0934</s0></fA01><fA02 i1="01"><s0>JVMEDH</s0></fA02><fA03 i2="1"><s0>J. virol. methods</s0></fA03><fA05><s2>179</s2></fA05><fA06><s2>2</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Detection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods</s1></fA08><fA11 i1="01" i2="1"><s1>NICHOLLS (John M.)</s1></fA11><fA11 i1="02" i2="1"><s1>WONG (Linda P. W.)</s1></fA11><fA11 i1="03" i2="1"><s1>CHAN (Renee W. Y.)</s1></fA11><fA11 i1="04" i2="1"><s1>POON (Leo L. M.)</s1></fA11><fA11 i1="05" i2="1"><s1>SO (Leo K. Y.)</s1></fA11><fA11 i1="06" i2="1"><s1>YEN (Hui-Ling)</s1></fA11><fA11 i1="07" i2="1"><s1>FUNG (Kevin)</s1></fA11><fA11 i1="08" i2="1"><s1>VAN POUCKE (Sjouke)</s1></fA11><fA11 i1="09" i2="1"><s1>MALIK PEIRIS (J. S.)</s1></fA11><fA14 i1="01"><s1>Department of Pathology, The University of Hong Kong, Pok Fu Lam</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></fA14><fA14 i1="02"><s1>Centre of Influenza Research, The University of Hong Kong</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ></fA14><fA14 i1="03"><s1>The HKU-Pasteur Research Centre</s1><s2>Hong Kong SAR</s2><s3>CHN</s3><sZ>6 aut.</sZ><sZ>9 aut.</sZ></fA14><fA14 i1="04"><s1>Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University</s1><s2>Merelbeke</s2><s3>BEL</s3><sZ>8 aut.</sZ></fA14><fA20><s1>409-413</s1></fA20><fA21><s1>2012</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>18295</s2><s5>354000506728080180</s5></fA43><fA44><s0>0000</s0><s1>© 2012 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>1/4 p.</s0></fA45><fA47 i1="01" i2="1"><s0>12-0082227</s0></fA47><fA60><s1>P</s1><s3>CC</s3></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Journal of virological methods</s0></fA64><fA66 i1="01"><s0>GBR</s0></fA66><fC01 i1="01" l="ENG"><s0>Tissues infected with highly pathogenic avian influenza viruses such as H5N1 and H7N7 are normally required to be fixed in formalin or paraformaldehyde before examination in order to inactivate the virus. In this study commercially available monoclonal antibodies to the influenza nucleoprotein (NP) were evaluated in order to determine which antibodies would identify positive cells in tissues fixed in formalin or paraformaldehyde. An assessment of which antigen retrieval process would unmask antigens blocked by formalin fixation was also made. Of six commercially available monoclonal antibodies tested, only one (HB65, European Veterinary Laboratories) was able to identify all formalin fixed avian, swine and human influenza virus infected tissues, and this was after pronase induced epitope retrieval. This monoclonal antibody is recommended for routine diagnostic use for the detection of influenza A infected tissues that have been fixed in formalin or paraformaldehyde.</s0></fC01><fC02 i1="01" i2="X"><s0>002A05C09</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Influenzavirus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Influenzavirus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Influenzavirus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Détection</s0><s5>05</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Detection</s0><s5>05</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Detección</s0><s5>05</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Immunohistochimie</s0><s5>06</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Immunohistochemistry</s0><s5>06</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Inmunohistoquímica</s0><s5>06</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Méthode</s0><s5>07</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Method</s0><s5>07</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Método</s0><s5>07</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Nucléoprotéine</s0><s5>08</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Nucleoprotein</s0><s5>08</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Nucleoproteina</s0><s5>08</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Grippe</s0><s5>14</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Influenza</s0><s5>14</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Gripe</s0><s5>14</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Orthomyxoviridae</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Orthomyxoviridae</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Orthomyxoviridae</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Virose</s0></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Viral disease</s0></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Virosis</s0></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Infection</s0></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Infection</s0></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Infección</s0></fC07><fN21><s1>065</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist><affiliations><list><country><li>Belgique</li><li>République populaire de Chine</li></country><region><li>Province de Flandre-Orientale</li><li>Région flamande</li></region><settlement><li>Gand</li></settlement><orgName><li>Université de Gand</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Nicholls, John M" sort="Nicholls, John M" uniqKey="Nicholls J" first="John M." last="Nicholls">John M. Nicholls</name></noRegion><name sortKey="Chan, Renee W Y" sort="Chan, Renee W Y" uniqKey="Chan R" first="Renee W. Y." last="Chan">Renee W. Y. Chan</name><name sortKey="Chan, Renee W Y" sort="Chan, Renee W Y" uniqKey="Chan R" first="Renee W. Y." last="Chan">Renee W. Y. Chan</name><name sortKey="Fung, Kevin" sort="Fung, Kevin" uniqKey="Fung K" first="Kevin" last="Fung">Kevin Fung</name><name sortKey="Malik Peiris, J S" sort="Malik Peiris, J S" uniqKey="Malik Peiris J" first="J. S." last="Malik Peiris">J. S. Malik Peiris</name><name sortKey="Malik Peiris, J S" sort="Malik Peiris, J S" uniqKey="Malik Peiris J" first="J. S." last="Malik Peiris">J. S. Malik Peiris</name><name sortKey="Nicholls, John M" sort="Nicholls, John M" uniqKey="Nicholls J" first="John M." last="Nicholls">John M. Nicholls</name><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name><name sortKey="So, Leo K Y" sort="So, Leo K Y" uniqKey="So L" first="Leo K. Y." last="So">Leo K. Y. So</name><name sortKey="Wong, Linda P W" sort="Wong, Linda P W" uniqKey="Wong L" first="Linda P. W." last="Wong">Linda P. W. Wong</name><name sortKey="Yen, Hui Ling" sort="Yen, Hui Ling" uniqKey="Yen H" first="Hui-Ling" last="Yen">Hui-Ling Yen</name><name sortKey="Yen, Hui Ling" sort="Yen, Hui Ling" uniqKey="Yen H" first="Hui-Ling" last="Yen">Hui-Ling Yen</name></country><country name="Belgique"><region name="Région flamande"><name sortKey="Van Poucke, Sjouke" sort="Van Poucke, Sjouke" uniqKey="Van Poucke S" first="Sjouke" last="Van Poucke">Sjouke Van Poucke</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Detection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods
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