Epitope Mapping of the Hemagglutinin Molecule of A/(H1N1)pdm09 Influenza Virus by Using Monoclonal Antibody Escape Mutants
Identifieur interne : 001908 ( Ncbi/Merge ); précédent : 001907; suivant : 001909Epitope Mapping of the Hemagglutinin Molecule of A/(H1N1)pdm09 Influenza Virus by Using Monoclonal Antibody Escape Mutants
Auteurs : Yoko Matsuzaki [Japon] ; Kanetsu Sugawara [Japon] ; Mina Nakauchi [Japon] ; Yoshimasa Takahashi [Japon] ; Taishi Onodera [Japon] ; Yasuko Tsunetsugu-Yokota [Japon] ; Takayuki Matsumura [Japon] ; Manabu Ato [Japon] ; Kazuo Kobayashi [Japon] ; Yoshitaka Shimotai [Japon] ; Katsumi Mizuta [Japon] ; Seiji Hongo [Japon] ; Masato Tashiro [Japon] ; Eri Nobusawa [Japon]Source :
- Journal of Virology [ 0022-538X ] ; 2014.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Analyse de séquence d'ADN, Animaux, Anticorps antiviraux (immunologie), Anticorps monoclonaux (immunologie), Cartographie épitopique (), Culture virale, Données de séquences moléculaires, Glycoprotéine hémagglutinine du virus influenza (génétique), Glycoprotéine hémagglutinine du virus influenza (immunologie), Protéines mutantes (génétique), Protéines mutantes (immunologie), Souris de lignée BALB C, Sous-type H1N1 du virus de la grippe A (génétique), Sous-type H1N1 du virus de la grippe A (immunologie), Sélection génétique, Tests d'inhibition de l'hémagglutination.
- MESH :
- génétique : ARN viral, Glycoprotéine hémagglutinine du virus influenza, Protéines mutantes, Sous-type H1N1 du virus de la grippe A.
- immunologie : Anticorps antiviraux, Anticorps monoclonaux, Glycoprotéine hémagglutinine du virus influenza, Protéines mutantes, Sous-type H1N1 du virus de la grippe A.
- Analyse de séquence d'ADN, Animaux, Cartographie épitopique, Culture virale, Données de séquences moléculaires, Souris de lignée BALB C, Sélection génétique, Tests d'inhibition de l'hémagglutination.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (immunology), Antibodies, Viral (immunology), Epitope Mapping (methods), Hemagglutination Inhibition Tests, Hemagglutinin Glycoproteins, Influenza Virus (genetics), Hemagglutinin Glycoproteins, Influenza Virus (immunology), Influenza A Virus, H1N1 Subtype (genetics), Influenza A Virus, H1N1 Subtype (immunology), Mice, Inbred BALB C, Molecular Sequence Data, Mutant Proteins (genetics), Mutant Proteins (immunology), RNA, Viral (genetics), Selection, Genetic, Sequence Analysis, DNA, Virus Cultivation.
- MESH :
- chemical , genetics : Hemagglutinin Glycoproteins, Influenza Virus, Mutant Proteins, RNA, Viral.
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Viral, Hemagglutinin Glycoproteins, Influenza Virus, Mutant Proteins.
- genetics : Influenza A Virus, H1N1 Subtype.
- immunology : Influenza A Virus, H1N1 Subtype.
- methods : Epitope Mapping.
- Animals, Hemagglutination Inhibition Tests, Mice, Inbred BALB C, Molecular Sequence Data, Selection, Genetic, Sequence Analysis, DNA, Virus Cultivation.
Abstract
We determined the antigenic structure of pandemic influenza A(H1N1)pdm09 virus hemagglutinin (HA) using 599 escape mutants that were selected using 16 anti-HA monoclonal antibodies (MAbs) against A/Narita/1/2009. The sequencing of mutant HA genes revealed 43 amino acid substitutions at 24 positions in three antigenic sites, Sa, Sb, and Ca2, which were previously mapped onto A/Puerto Rico/8/34 (A/PR/8/34) HA (A. J. Caton, G. G. Brownlee, J. W. Yewdell, and W. Gerhard, Cell 31:417–427, 1982), and an undesignated site, i.e., amino acid residues 141, 142, 143, 171, 172, 174, 177, and 180 in the Sa site, residues 170, 173, 202, 206, 210, 211, and 212 in the Sb site, residues 151, 154, 156, 157, 158, 159, 200, and 238 in the Ca2 site, and residue 147 in the undesignated site (numbering begins at the first methionine). Sixteen MAbs were classified into four groups based on their cross-reactivity with the panel of escape mutants in the hemagglutination inhibition test. Among them, six MAbs targeting the Sa and Sb sites recognized both residues at positions 172 and 173. MAb n2 lost reactivity when mutations were introduced at positions 147, 159 (site Ca2), 170 (site Sb), and 172 (site Sa). We designated the site consisting of these residues as site Pa. From 2009 to 2013, no antigenic drift was detected for the A(H1N1)pdm09 viruses. However, if a novel variant carrying a mutation at a position involved in the epitopes of several MAbs, such as 172, appeared, such a virus would have the advantage of becoming a drift strain.
Url:
DOI: 10.1128/JVI.01381-14
PubMed: 25122788
PubMed Central: 4248900
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000745
- to stream Pmc, to step Curation: 000745
- to stream Pmc, to step Checkpoint: 000477
- to stream PubMed, to step Corpus: 000774
- to stream PubMed, to step Curation: 000774
- to stream PubMed, to step Checkpoint: 000814
Links to Exploration step
PMC:4248900Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Epitope Mapping of the Hemagglutinin Molecule of A/(H1N1)pdm09 Influenza Virus by Using Monoclonal Antibody Escape Mutants</title>
<author><name sortKey="Matsuzaki, Yoko" sort="Matsuzaki, Yoko" uniqKey="Matsuzaki Y" first="Yoko" last="Matsuzaki">Yoko Matsuzaki</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sugawara, Kanetsu" sort="Sugawara, Kanetsu" uniqKey="Sugawara K" first="Kanetsu" last="Sugawara">Kanetsu Sugawara</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nakauchi, Mina" sort="Nakauchi, Mina" uniqKey="Nakauchi M" first="Mina" last="Nakauchi">Mina Nakauchi</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Takahashi, Yoshimasa" sort="Takahashi, Yoshimasa" uniqKey="Takahashi Y" first="Yoshimasa" last="Takahashi">Yoshimasa Takahashi</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Onodera, Taishi" sort="Onodera, Taishi" uniqKey="Onodera T" first="Taishi" last="Onodera">Taishi Onodera</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Tsunetsugu Yokota, Yasuko" sort="Tsunetsugu Yokota, Yasuko" uniqKey="Tsunetsugu Yokota Y" first="Yasuko" last="Tsunetsugu-Yokota">Yasuko Tsunetsugu-Yokota</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Matsumura, Takayuki" sort="Matsumura, Takayuki" uniqKey="Matsumura T" first="Takayuki" last="Matsumura">Takayuki Matsumura</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ato, Manabu" sort="Ato, Manabu" uniqKey="Ato M" first="Manabu" last="Ato">Manabu Ato</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kobayashi, Kazuo" sort="Kobayashi, Kazuo" uniqKey="Kobayashi K" first="Kazuo" last="Kobayashi">Kazuo Kobayashi</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Shimotai, Yoshitaka" sort="Shimotai, Yoshitaka" uniqKey="Shimotai Y" first="Yoshitaka" last="Shimotai">Yoshitaka Shimotai</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mizuta, Katsumi" sort="Mizuta, Katsumi" uniqKey="Mizuta K" first="Katsumi" last="Mizuta">Katsumi Mizuta</name>
<affiliation wicri:level="1"><nlm:aff id="aff4">Department of Microbiology, Yamagata Prefectural Institute of Public Health, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Microbiology, Yamagata Prefectural Institute of Public Health, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hongo, Seiji" sort="Hongo, Seiji" uniqKey="Hongo S" first="Seiji" last="Hongo">Seiji Hongo</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nobusawa, Eri" sort="Nobusawa, Eri" uniqKey="Nobusawa E" first="Eri" last="Nobusawa">Eri Nobusawa</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">25122788</idno>
<idno type="pmc">4248900</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4248900</idno>
<idno type="RBID">PMC:4248900</idno>
<idno type="doi">10.1128/JVI.01381-14</idno>
<date when="2014">2014</date>
<idno type="wicri:Area/Pmc/Corpus">000745</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000745</idno>
<idno type="wicri:Area/Pmc/Curation">000745</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000745</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000477</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000477</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:25122788</idno>
<idno type="wicri:Area/PubMed/Corpus">000774</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000774</idno>
<idno type="wicri:Area/PubMed/Curation">000774</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000774</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000814</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000814</idno>
<idno type="wicri:Area/Ncbi/Merge">001908</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Epitope Mapping of the Hemagglutinin Molecule of A/(H1N1)pdm09 Influenza Virus by Using Monoclonal Antibody Escape Mutants</title>
<author><name sortKey="Matsuzaki, Yoko" sort="Matsuzaki, Yoko" uniqKey="Matsuzaki Y" first="Yoko" last="Matsuzaki">Yoko Matsuzaki</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sugawara, Kanetsu" sort="Sugawara, Kanetsu" uniqKey="Sugawara K" first="Kanetsu" last="Sugawara">Kanetsu Sugawara</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nakauchi, Mina" sort="Nakauchi, Mina" uniqKey="Nakauchi M" first="Mina" last="Nakauchi">Mina Nakauchi</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Takahashi, Yoshimasa" sort="Takahashi, Yoshimasa" uniqKey="Takahashi Y" first="Yoshimasa" last="Takahashi">Yoshimasa Takahashi</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Onodera, Taishi" sort="Onodera, Taishi" uniqKey="Onodera T" first="Taishi" last="Onodera">Taishi Onodera</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Tsunetsugu Yokota, Yasuko" sort="Tsunetsugu Yokota, Yasuko" uniqKey="Tsunetsugu Yokota Y" first="Yasuko" last="Tsunetsugu-Yokota">Yasuko Tsunetsugu-Yokota</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Matsumura, Takayuki" sort="Matsumura, Takayuki" uniqKey="Matsumura T" first="Takayuki" last="Matsumura">Takayuki Matsumura</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ato, Manabu" sort="Ato, Manabu" uniqKey="Ato M" first="Manabu" last="Ato">Manabu Ato</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kobayashi, Kazuo" sort="Kobayashi, Kazuo" uniqKey="Kobayashi K" first="Kazuo" last="Kobayashi">Kazuo Kobayashi</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Shimotai, Yoshitaka" sort="Shimotai, Yoshitaka" uniqKey="Shimotai Y" first="Yoshitaka" last="Shimotai">Yoshitaka Shimotai</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mizuta, Katsumi" sort="Mizuta, Katsumi" uniqKey="Mizuta K" first="Katsumi" last="Mizuta">Katsumi Mizuta</name>
<affiliation wicri:level="1"><nlm:aff id="aff4">Department of Microbiology, Yamagata Prefectural Institute of Public Health, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Microbiology, Yamagata Prefectural Institute of Public Health, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hongo, Seiji" sort="Hongo, Seiji" uniqKey="Hongo S" first="Seiji" last="Hongo">Seiji Hongo</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nobusawa, Eri" sort="Nobusawa, Eri" uniqKey="Nobusawa E" first="Eri" last="Nobusawa">Eri Nobusawa</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antibodies, Monoclonal (immunology)</term>
<term>Antibodies, Viral (immunology)</term>
<term>Epitope Mapping (methods)</term>
<term>Hemagglutination Inhibition Tests</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (genetics)</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (immunology)</term>
<term>Influenza A Virus, H1N1 Subtype (genetics)</term>
<term>Influenza A Virus, H1N1 Subtype (immunology)</term>
<term>Mice, Inbred BALB C</term>
<term>Molecular Sequence Data</term>
<term>Mutant Proteins (genetics)</term>
<term>Mutant Proteins (immunology)</term>
<term>RNA, Viral (genetics)</term>
<term>Selection, Genetic</term>
<term>Sequence Analysis, DNA</term>
<term>Virus Cultivation</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term>
<term>Analyse de séquence d'ADN</term>
<term>Animaux</term>
<term>Anticorps antiviraux (immunologie)</term>
<term>Anticorps monoclonaux (immunologie)</term>
<term>Cartographie épitopique ()</term>
<term>Culture virale</term>
<term>Données de séquences moléculaires</term>
<term>Glycoprotéine hémagglutinine du virus influenza (génétique)</term>
<term>Glycoprotéine hémagglutinine du virus influenza (immunologie)</term>
<term>Protéines mutantes (génétique)</term>
<term>Protéines mutantes (immunologie)</term>
<term>Souris de lignée BALB C</term>
<term>Sous-type H1N1 du virus de la grippe A (génétique)</term>
<term>Sous-type H1N1 du virus de la grippe A (immunologie)</term>
<term>Sélection génétique</term>
<term>Tests d'inhibition de l'hémagglutination</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Hemagglutinin Glycoproteins, Influenza Virus</term>
<term>Mutant Proteins</term>
<term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term>
<term>Antibodies, Viral</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus</term>
<term>Mutant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term>
<term>Glycoprotéine hémagglutinine du virus influenza</term>
<term>Protéines mutantes</term>
<term>Sous-type H1N1 du virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Anticorps monoclonaux</term>
<term>Glycoprotéine hémagglutinine du virus influenza</term>
<term>Protéines mutantes</term>
<term>Sous-type H1N1 du virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Epitope Mapping</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Hemagglutination Inhibition Tests</term>
<term>Mice, Inbred BALB C</term>
<term>Molecular Sequence Data</term>
<term>Selection, Genetic</term>
<term>Sequence Analysis, DNA</term>
<term>Virus Cultivation</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term>
<term>Animaux</term>
<term>Cartographie épitopique</term>
<term>Culture virale</term>
<term>Données de séquences moléculaires</term>
<term>Souris de lignée BALB C</term>
<term>Sélection génétique</term>
<term>Tests d'inhibition de l'hémagglutination</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>ABSTRACT</title>
<p>We determined the antigenic structure of pandemic influenza A(H1N1)pdm09 virus hemagglutinin (HA) using 599 escape mutants that were selected using 16 anti-HA monoclonal antibodies (MAbs) against A/Narita/1/2009. The sequencing of mutant HA genes revealed 43 amino acid substitutions at 24 positions in three antigenic sites, Sa, Sb, and Ca2, which were previously mapped onto A/Puerto Rico/8/34 (A/PR/8/34) HA (A. J. Caton, G. G. Brownlee, J. W. Yewdell, and W. Gerhard, Cell 31:417–427, 1982), and an undesignated site, i.e., amino acid residues 141, 142, 143, 171, 172, 174, 177, and 180 in the Sa site, residues 170, 173, 202, 206, 210, 211, and 212 in the Sb site, residues 151, 154, 156, 157, 158, 159, 200, and 238 in the Ca2 site, and residue 147 in the undesignated site (numbering begins at the first methionine). Sixteen MAbs were classified into four groups based on their cross-reactivity with the panel of escape mutants in the hemagglutination inhibition test. Among them, six MAbs targeting the Sa and Sb sites recognized both residues at positions 172 and 173. MAb n2 lost reactivity when mutations were introduced at positions 147, 159 (site Ca2), 170 (site Sb), and 172 (site Sa). We designated the site consisting of these residues as site Pa. From 2009 to 2013, no antigenic drift was detected for the A(H1N1)pdm09 viruses. However, if a novel variant carrying a mutation at a position involved in the epitopes of several MAbs, such as 172, appeared, such a virus would have the advantage of becoming a drift strain.</p>
<p><bold>IMPORTANCE</bold>
The first influenza pandemic of the 21st century occurred in 2009 with the emergence of a novel virus originating with swine influenza, A(H1N1)pdm09. Although HA of A(H1N1)pdm09 has a common origin (1918 H1N1) with seasonal H1N1, the antigenic divergence of HA between the seasonal H1N1 and A(H1N1)pdm09 viruses gave rise to the influenza pandemic in 2009. To take precautions against the antigenic drift of the A(H1N1)pdm09 virus in the near future, it is important to identify its precise antigenic structure. To obtain various mutants that are not neutralized by MAbs, it is important to neutralize several plaque-cloned parent viruses rather than only a single parent virus. We characterized 599 escape mutants that were obtained by neutralizing four parent viruses of A(H1N1)pdm09 in the presence of 16 MAbs. Consequently, we were able to determine the details of the antigenic structure of HA, including a novel epitope.</p>
</div>
</front>
</TEI>
<double pmid="25122788"><pmc><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Epitope Mapping of the Hemagglutinin Molecule of A/(H1N1)pdm09 Influenza Virus by Using Monoclonal Antibody Escape Mutants</title>
<author><name sortKey="Matsuzaki, Yoko" sort="Matsuzaki, Yoko" uniqKey="Matsuzaki Y" first="Yoko" last="Matsuzaki">Yoko Matsuzaki</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sugawara, Kanetsu" sort="Sugawara, Kanetsu" uniqKey="Sugawara K" first="Kanetsu" last="Sugawara">Kanetsu Sugawara</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nakauchi, Mina" sort="Nakauchi, Mina" uniqKey="Nakauchi M" first="Mina" last="Nakauchi">Mina Nakauchi</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Takahashi, Yoshimasa" sort="Takahashi, Yoshimasa" uniqKey="Takahashi Y" first="Yoshimasa" last="Takahashi">Yoshimasa Takahashi</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Onodera, Taishi" sort="Onodera, Taishi" uniqKey="Onodera T" first="Taishi" last="Onodera">Taishi Onodera</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Tsunetsugu Yokota, Yasuko" sort="Tsunetsugu Yokota, Yasuko" uniqKey="Tsunetsugu Yokota Y" first="Yasuko" last="Tsunetsugu-Yokota">Yasuko Tsunetsugu-Yokota</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Matsumura, Takayuki" sort="Matsumura, Takayuki" uniqKey="Matsumura T" first="Takayuki" last="Matsumura">Takayuki Matsumura</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ato, Manabu" sort="Ato, Manabu" uniqKey="Ato M" first="Manabu" last="Ato">Manabu Ato</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kobayashi, Kazuo" sort="Kobayashi, Kazuo" uniqKey="Kobayashi K" first="Kazuo" last="Kobayashi">Kazuo Kobayashi</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Shimotai, Yoshitaka" sort="Shimotai, Yoshitaka" uniqKey="Shimotai Y" first="Yoshitaka" last="Shimotai">Yoshitaka Shimotai</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mizuta, Katsumi" sort="Mizuta, Katsumi" uniqKey="Mizuta K" first="Katsumi" last="Mizuta">Katsumi Mizuta</name>
<affiliation wicri:level="1"><nlm:aff id="aff4">Department of Microbiology, Yamagata Prefectural Institute of Public Health, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Microbiology, Yamagata Prefectural Institute of Public Health, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hongo, Seiji" sort="Hongo, Seiji" uniqKey="Hongo S" first="Seiji" last="Hongo">Seiji Hongo</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nobusawa, Eri" sort="Nobusawa, Eri" uniqKey="Nobusawa E" first="Eri" last="Nobusawa">Eri Nobusawa</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">25122788</idno>
<idno type="pmc">4248900</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4248900</idno>
<idno type="RBID">PMC:4248900</idno>
<idno type="doi">10.1128/JVI.01381-14</idno>
<date when="2014">2014</date>
<idno type="wicri:Area/Pmc/Corpus">000745</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000745</idno>
<idno type="wicri:Area/Pmc/Curation">000745</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000745</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000477</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000477</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Epitope Mapping of the Hemagglutinin Molecule of A/(H1N1)pdm09 Influenza Virus by Using Monoclonal Antibody Escape Mutants</title>
<author><name sortKey="Matsuzaki, Yoko" sort="Matsuzaki, Yoko" uniqKey="Matsuzaki Y" first="Yoko" last="Matsuzaki">Yoko Matsuzaki</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sugawara, Kanetsu" sort="Sugawara, Kanetsu" uniqKey="Sugawara K" first="Kanetsu" last="Sugawara">Kanetsu Sugawara</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nakauchi, Mina" sort="Nakauchi, Mina" uniqKey="Nakauchi M" first="Mina" last="Nakauchi">Mina Nakauchi</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Takahashi, Yoshimasa" sort="Takahashi, Yoshimasa" uniqKey="Takahashi Y" first="Yoshimasa" last="Takahashi">Yoshimasa Takahashi</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Onodera, Taishi" sort="Onodera, Taishi" uniqKey="Onodera T" first="Taishi" last="Onodera">Taishi Onodera</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Tsunetsugu Yokota, Yasuko" sort="Tsunetsugu Yokota, Yasuko" uniqKey="Tsunetsugu Yokota Y" first="Yasuko" last="Tsunetsugu-Yokota">Yasuko Tsunetsugu-Yokota</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Matsumura, Takayuki" sort="Matsumura, Takayuki" uniqKey="Matsumura T" first="Takayuki" last="Matsumura">Takayuki Matsumura</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ato, Manabu" sort="Ato, Manabu" uniqKey="Ato M" first="Manabu" last="Ato">Manabu Ato</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kobayashi, Kazuo" sort="Kobayashi, Kazuo" uniqKey="Kobayashi K" first="Kazuo" last="Kobayashi">Kazuo Kobayashi</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Shimotai, Yoshitaka" sort="Shimotai, Yoshitaka" uniqKey="Shimotai Y" first="Yoshitaka" last="Shimotai">Yoshitaka Shimotai</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mizuta, Katsumi" sort="Mizuta, Katsumi" uniqKey="Mizuta K" first="Katsumi" last="Mizuta">Katsumi Mizuta</name>
<affiliation wicri:level="1"><nlm:aff id="aff4">Department of Microbiology, Yamagata Prefectural Institute of Public Health, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Microbiology, Yamagata Prefectural Institute of Public Health, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hongo, Seiji" sort="Hongo, Seiji" uniqKey="Hongo S" first="Seiji" last="Hongo">Seiji Hongo</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nobusawa, Eri" sort="Nobusawa, Eri" uniqKey="Nobusawa E" first="Eri" last="Nobusawa">Eri Nobusawa</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>ABSTRACT</title>
<p>We determined the antigenic structure of pandemic influenza A(H1N1)pdm09 virus hemagglutinin (HA) using 599 escape mutants that were selected using 16 anti-HA monoclonal antibodies (MAbs) against A/Narita/1/2009. The sequencing of mutant HA genes revealed 43 amino acid substitutions at 24 positions in three antigenic sites, Sa, Sb, and Ca2, which were previously mapped onto A/Puerto Rico/8/34 (A/PR/8/34) HA (A. J. Caton, G. G. Brownlee, J. W. Yewdell, and W. Gerhard, Cell 31:417–427, 1982), and an undesignated site, i.e., amino acid residues 141, 142, 143, 171, 172, 174, 177, and 180 in the Sa site, residues 170, 173, 202, 206, 210, 211, and 212 in the Sb site, residues 151, 154, 156, 157, 158, 159, 200, and 238 in the Ca2 site, and residue 147 in the undesignated site (numbering begins at the first methionine). Sixteen MAbs were classified into four groups based on their cross-reactivity with the panel of escape mutants in the hemagglutination inhibition test. Among them, six MAbs targeting the Sa and Sb sites recognized both residues at positions 172 and 173. MAb n2 lost reactivity when mutations were introduced at positions 147, 159 (site Ca2), 170 (site Sb), and 172 (site Sa). We designated the site consisting of these residues as site Pa. From 2009 to 2013, no antigenic drift was detected for the A(H1N1)pdm09 viruses. However, if a novel variant carrying a mutation at a position involved in the epitopes of several MAbs, such as 172, appeared, such a virus would have the advantage of becoming a drift strain.</p>
<p><bold>IMPORTANCE</bold>
The first influenza pandemic of the 21st century occurred in 2009 with the emergence of a novel virus originating with swine influenza, A(H1N1)pdm09. Although HA of A(H1N1)pdm09 has a common origin (1918 H1N1) with seasonal H1N1, the antigenic divergence of HA between the seasonal H1N1 and A(H1N1)pdm09 viruses gave rise to the influenza pandemic in 2009. To take precautions against the antigenic drift of the A(H1N1)pdm09 virus in the near future, it is important to identify its precise antigenic structure. To obtain various mutants that are not neutralized by MAbs, it is important to neutralize several plaque-cloned parent viruses rather than only a single parent virus. We characterized 599 escape mutants that were obtained by neutralizing four parent viruses of A(H1N1)pdm09 in the presence of 16 MAbs. Consequently, we were able to determine the details of the antigenic structure of HA, including a novel epitope.</p>
</div>
</front>
</TEI>
</pmc>
<pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Epitope mapping of the hemagglutinin molecule of A/(H1N1)pdm09 influenza virus by using monoclonal antibody escape mutants.</title>
<author><name sortKey="Matsuzaki, Yoko" sort="Matsuzaki, Yoko" uniqKey="Matsuzaki Y" first="Yoko" last="Matsuzaki">Yoko Matsuzaki</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sugawara, Kanetsu" sort="Sugawara, Kanetsu" uniqKey="Sugawara K" first="Kanetsu" last="Sugawara">Kanetsu Sugawara</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nakauchi, Mina" sort="Nakauchi, Mina" uniqKey="Nakauchi M" first="Mina" last="Nakauchi">Mina Nakauchi</name>
<affiliation wicri:level="3"><nlm:affiliation>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Takahashi, Yoshimasa" sort="Takahashi, Yoshimasa" uniqKey="Takahashi Y" first="Yoshimasa" last="Takahashi">Yoshimasa Takahashi</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Onodera, Taishi" sort="Onodera, Taishi" uniqKey="Onodera T" first="Taishi" last="Onodera">Taishi Onodera</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Tsunetsugu Yokota, Yasuko" sort="Tsunetsugu Yokota, Yasuko" uniqKey="Tsunetsugu Yokota Y" first="Yasuko" last="Tsunetsugu-Yokota">Yasuko Tsunetsugu-Yokota</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Matsumura, Takayuki" sort="Matsumura, Takayuki" uniqKey="Matsumura T" first="Takayuki" last="Matsumura">Takayuki Matsumura</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ato, Manabu" sort="Ato, Manabu" uniqKey="Ato M" first="Manabu" last="Ato">Manabu Ato</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kobayashi, Kazuo" sort="Kobayashi, Kazuo" uniqKey="Kobayashi K" first="Kazuo" last="Kobayashi">Kazuo Kobayashi</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Shimotai, Yoshitaka" sort="Shimotai, Yoshitaka" uniqKey="Shimotai Y" first="Yoshitaka" last="Shimotai">Yoshitaka Shimotai</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mizuta, Katsumi" sort="Mizuta, Katsumi" uniqKey="Mizuta K" first="Katsumi" last="Mizuta">Katsumi Mizuta</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, Yamagata Prefectural Institute of Public Health, Yamagata, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Microbiology, Yamagata Prefectural Institute of Public Health, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hongo, Seiji" sort="Hongo, Seiji" uniqKey="Hongo S" first="Seiji" last="Hongo">Seiji Hongo</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name>
<affiliation wicri:level="3"><nlm:affiliation>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nobusawa, Eri" sort="Nobusawa, Eri" uniqKey="Nobusawa E" first="Eri" last="Nobusawa">Eri Nobusawa</name>
<affiliation wicri:level="3"><nlm:affiliation>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan nobusawa@nih.go.jp.</nlm:affiliation>
<country wicri:rule="url">Japon</country>
<wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2014">2014</date>
<idno type="RBID">pubmed:25122788</idno>
<idno type="pmid">25122788</idno>
<idno type="doi">10.1128/JVI.01381-14</idno>
<idno type="wicri:Area/PubMed/Corpus">000774</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000774</idno>
<idno type="wicri:Area/PubMed/Curation">000774</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000774</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000814</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000814</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Epitope mapping of the hemagglutinin molecule of A/(H1N1)pdm09 influenza virus by using monoclonal antibody escape mutants.</title>
<author><name sortKey="Matsuzaki, Yoko" sort="Matsuzaki, Yoko" uniqKey="Matsuzaki Y" first="Yoko" last="Matsuzaki">Yoko Matsuzaki</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sugawara, Kanetsu" sort="Sugawara, Kanetsu" uniqKey="Sugawara K" first="Kanetsu" last="Sugawara">Kanetsu Sugawara</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nakauchi, Mina" sort="Nakauchi, Mina" uniqKey="Nakauchi M" first="Mina" last="Nakauchi">Mina Nakauchi</name>
<affiliation wicri:level="3"><nlm:affiliation>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Takahashi, Yoshimasa" sort="Takahashi, Yoshimasa" uniqKey="Takahashi Y" first="Yoshimasa" last="Takahashi">Yoshimasa Takahashi</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Onodera, Taishi" sort="Onodera, Taishi" uniqKey="Onodera T" first="Taishi" last="Onodera">Taishi Onodera</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Tsunetsugu Yokota, Yasuko" sort="Tsunetsugu Yokota, Yasuko" uniqKey="Tsunetsugu Yokota Y" first="Yasuko" last="Tsunetsugu-Yokota">Yasuko Tsunetsugu-Yokota</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Matsumura, Takayuki" sort="Matsumura, Takayuki" uniqKey="Matsumura T" first="Takayuki" last="Matsumura">Takayuki Matsumura</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ato, Manabu" sort="Ato, Manabu" uniqKey="Ato M" first="Manabu" last="Ato">Manabu Ato</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kobayashi, Kazuo" sort="Kobayashi, Kazuo" uniqKey="Kobayashi K" first="Kazuo" last="Kobayashi">Kazuo Kobayashi</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Shimotai, Yoshitaka" sort="Shimotai, Yoshitaka" uniqKey="Shimotai Y" first="Yoshitaka" last="Shimotai">Yoshitaka Shimotai</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mizuta, Katsumi" sort="Mizuta, Katsumi" uniqKey="Mizuta K" first="Katsumi" last="Mizuta">Katsumi Mizuta</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, Yamagata Prefectural Institute of Public Health, Yamagata, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Microbiology, Yamagata Prefectural Institute of Public Health, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hongo, Seiji" sort="Hongo, Seiji" uniqKey="Hongo S" first="Seiji" last="Hongo">Seiji Hongo</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Infectious Diseases, Yamagata University Faculty of Medicine, Yamagata</wicri:regionArea>
<wicri:noRegion>Yamagata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name>
<affiliation wicri:level="3"><nlm:affiliation>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nobusawa, Eri" sort="Nobusawa, Eri" uniqKey="Nobusawa E" first="Eri" last="Nobusawa">Eri Nobusawa</name>
<affiliation wicri:level="3"><nlm:affiliation>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan nobusawa@nih.go.jp.</nlm:affiliation>
<country wicri:rule="url">Japon</country>
<wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of virology</title>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="2014" type="published">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antibodies, Monoclonal (immunology)</term>
<term>Antibodies, Viral (immunology)</term>
<term>Epitope Mapping (methods)</term>
<term>Hemagglutination Inhibition Tests</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (genetics)</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (immunology)</term>
<term>Influenza A Virus, H1N1 Subtype (genetics)</term>
<term>Influenza A Virus, H1N1 Subtype (immunology)</term>
<term>Mice, Inbred BALB C</term>
<term>Molecular Sequence Data</term>
<term>Mutant Proteins (genetics)</term>
<term>Mutant Proteins (immunology)</term>
<term>RNA, Viral (genetics)</term>
<term>Selection, Genetic</term>
<term>Sequence Analysis, DNA</term>
<term>Virus Cultivation</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term>
<term>Analyse de séquence d'ADN</term>
<term>Animaux</term>
<term>Anticorps antiviraux (immunologie)</term>
<term>Anticorps monoclonaux (immunologie)</term>
<term>Cartographie épitopique ()</term>
<term>Culture virale</term>
<term>Données de séquences moléculaires</term>
<term>Glycoprotéine hémagglutinine du virus influenza (génétique)</term>
<term>Glycoprotéine hémagglutinine du virus influenza (immunologie)</term>
<term>Protéines mutantes (génétique)</term>
<term>Protéines mutantes (immunologie)</term>
<term>Souris de lignée BALB C</term>
<term>Sous-type H1N1 du virus de la grippe A (génétique)</term>
<term>Sous-type H1N1 du virus de la grippe A (immunologie)</term>
<term>Sélection génétique</term>
<term>Tests d'inhibition de l'hémagglutination</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Hemagglutinin Glycoproteins, Influenza Virus</term>
<term>Mutant Proteins</term>
<term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term>
<term>Antibodies, Viral</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus</term>
<term>Mutant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term>
<term>Glycoprotéine hémagglutinine du virus influenza</term>
<term>Protéines mutantes</term>
<term>Sous-type H1N1 du virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Anticorps monoclonaux</term>
<term>Glycoprotéine hémagglutinine du virus influenza</term>
<term>Protéines mutantes</term>
<term>Sous-type H1N1 du virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Epitope Mapping</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Hemagglutination Inhibition Tests</term>
<term>Mice, Inbred BALB C</term>
<term>Molecular Sequence Data</term>
<term>Selection, Genetic</term>
<term>Sequence Analysis, DNA</term>
<term>Virus Cultivation</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term>
<term>Animaux</term>
<term>Cartographie épitopique</term>
<term>Culture virale</term>
<term>Données de séquences moléculaires</term>
<term>Souris de lignée BALB C</term>
<term>Sélection génétique</term>
<term>Tests d'inhibition de l'hémagglutination</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">We determined the antigenic structure of pandemic influenza A(H1N1)pdm09 virus hemagglutinin (HA) using 599 escape mutants that were selected using 16 anti-HA monoclonal antibodies (MAbs) against A/Narita/1/2009. The sequencing of mutant HA genes revealed 43 amino acid substitutions at 24 positions in three antigenic sites, Sa, Sb, and Ca2, which were previously mapped onto A/Puerto Rico/8/34 (A/PR/8/34) HA (A. J. Caton, G. G. Brownlee, J. W. Yewdell, and W. Gerhard, Cell 31:417-427, 1982), and an undesignated site, i.e., amino acid residues 141, 142, 143, 171, 172, 174, 177, and 180 in the Sa site, residues 170, 173, 202, 206, 210, 211, and 212 in the Sb site, residues 151, 154, 156, 157, 158, 159, 200, and 238 in the Ca2 site, and residue 147 in the undesignated site (numbering begins at the first methionine). Sixteen MAbs were classified into four groups based on their cross-reactivity with the panel of escape mutants in the hemagglutination inhibition test. Among them, six MAbs targeting the Sa and Sb sites recognized both residues at positions 172 and 173. MAb n2 lost reactivity when mutations were introduced at positions 147, 159 (site Ca2), 170 (site Sb), and 172 (site Sa). We designated the site consisting of these residues as site Pa. From 2009 to 2013, no antigenic drift was detected for the A(H1N1)pdm09 viruses. However, if a novel variant carrying a mutation at a position involved in the epitopes of several MAbs, such as 172, appeared, such a virus would have the advantage of becoming a drift strain.</div>
</front>
</TEI>
</pubmed>
</double>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/PandemieGrippaleV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001908 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 001908 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= PandemieGrippaleV1 |flux= Ncbi |étape= Merge |type= RBID |clé= PMC:4248900 |texte= Epitope Mapping of the Hemagglutinin Molecule of A/(H1N1)pdm09 Influenza Virus by Using Monoclonal Antibody Escape Mutants }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:25122788" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \ | NlmPubMed2Wicri -a PandemieGrippaleV1
![]() | This area was generated with Dilib version V0.6.34. | ![]() |