Dimeric FcγR Ectodomains as Probes of the Fc Receptor Function of Anti-Influenza Virus IgG.
Identifieur interne : 001B72 ( Ncbi/Curation ); précédent : 001B71; suivant : 001B73Dimeric FcγR Ectodomains as Probes of the Fc Receptor Function of Anti-Influenza Virus IgG.
Auteurs : Bruce D. Wines [Australie] ; Hillary A. Vanderven [Australie] ; Sandra E. Esparon [Australie] ; Anne B. Kristensen [Australie] ; Stephen J. Kent [Australie] ; P Mark Hogarth [Australie]Source :
- Journal of immunology (Baltimore, Md. : 1950) [ 1550-6606 ] ; 2016.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (analyse), Anticorps antiviraux (immunologie), Complexe antigène-anticorps (immunologie), Humains, Immunoglobuline G (analyse), Immunoglobuline G (immunologie), Récepteur Fc (immunologie), Récepteurs du fragment Fc des IgG (immunologie), Sous-type H1N1 du virus de la grippe A, Techniques immunologiques ().
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Antibodies, Viral, Immunoglobulin G.
- chemical , immunology : Antibodies, Viral, Antigen-Antibody Complex, Immunoglobulin G, Receptors, Fc, Receptors, IgG.
- methods : Immunologic Techniques.
- Humans, Influenza A Virus, H1N1 Subtype.
Abstract
Ab-dependent cellular cytotoxicity, phagocytosis, and Ag presentation are key mechanisms of action of Abs arising in vaccine or naturally acquired immunity, as well of therapeutic mAbs. Cells expressing the low-affinity FcγRs (FcγRII or CD32 and FcγRIII or CD16) are activated for these functions when receptors are aggregated following the binding of IgG-opsonized targets. Despite the diversity of the Fc receptor proteins, IgG ligands, and potential responding cell types, the induction of all FcγR-mediated responses by opsonized targets requires the presentation of multiple Fc regions in close proximity to each other. We demonstrated that such "near-neighbor" Fc regions can be detected using defined recombinant soluble (rs) dimeric low-affinity ectodomains (rsFcγR) that have an absolute binding requirement for the simultaneous engagement of two IgG Fc regions. Like cell surface-expressed FcγRs, the binding of dimeric rsFcγR ectodomains to Ab immune complexes was affected by Ab subclass, presentation, opsonization density, Fc fucosylation, or mutation. The activation of an NK cell line and primary NK cells by human IgG-opsonized influenza A hemagglutinin correlated with dimeric rsFcγRIIIa binding activity but not with Ab titer. Furthermore, the dimeric rsFcγR binding assay sensitively detected greater Fc receptor activity to pandemic H1N1 hemagglutinin after the swine influenza pandemic of 2009 in pooled human polyclonal IgG. Thus these dimeric rsFcγR ectodomains are validated, defined probes that should prove valuable in measuring the immune-activating capacity of IgG Abs elicited by infection or vaccination or experimentally derived IgG and its variants.
DOI: 10.4049/jimmunol.1502551
PubMed: 27385782
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Cells expressing the low-affinity FcγRs (FcγRII or CD32 and FcγRIII or CD16) are activated for these functions when receptors are aggregated following the binding of IgG-opsonized targets. Despite the diversity of the Fc receptor proteins, IgG ligands, and potential responding cell types, the induction of all FcγR-mediated responses by opsonized targets requires the presentation of multiple Fc regions in close proximity to each other. We demonstrated that such "near-neighbor" Fc regions can be detected using defined recombinant soluble (rs) dimeric low-affinity ectodomains (rsFcγR) that have an absolute binding requirement for the simultaneous engagement of two IgG Fc regions. Like cell surface-expressed FcγRs, the binding of dimeric rsFcγR ectodomains to Ab immune complexes was affected by Ab subclass, presentation, opsonization density, Fc fucosylation, or mutation. The activation of an NK cell line and primary NK cells by human IgG-opsonized influenza A hemagglutinin correlated with dimeric rsFcγRIIIa binding activity but not with Ab titer. Furthermore, the dimeric rsFcγR binding assay sensitively detected greater Fc receptor activity to pandemic H1N1 hemagglutinin after the swine influenza pandemic of 2009 in pooled human polyclonal IgG. Thus these dimeric rsFcγR ectodomains are validated, defined probes that should prove valuable in measuring the immune-activating capacity of IgG Abs elicited by infection or vaccination or experimentally derived IgG and its variants.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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