Dimeric FcγR Ectodomains as Probes of the Fc Receptor Function of Anti-Influenza Virus IgG.
Identifieur interne : 001B72 ( Ncbi/Curation ); précédent : 001B71; suivant : 001B73Dimeric FcγR Ectodomains as Probes of the Fc Receptor Function of Anti-Influenza Virus IgG.
Auteurs : Bruce D. Wines [Australie] ; Hillary A. Vanderven [Australie] ; Sandra E. Esparon [Australie] ; Anne B. Kristensen [Australie] ; Stephen J. Kent [Australie] ; P Mark Hogarth [Australie]Source :
- Journal of immunology (Baltimore, Md. : 1950) [ 1550-6606 ] ; 2016.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (analyse), Anticorps antiviraux (immunologie), Complexe antigène-anticorps (immunologie), Humains, Immunoglobuline G (analyse), Immunoglobuline G (immunologie), Récepteur Fc (immunologie), Récepteurs du fragment Fc des IgG (immunologie), Sous-type H1N1 du virus de la grippe A, Techniques immunologiques ().
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Antibodies, Viral, Immunoglobulin G.
- chemical , immunology : Antibodies, Viral, Antigen-Antibody Complex, Immunoglobulin G, Receptors, Fc, Receptors, IgG.
- methods : Immunologic Techniques.
- Humans, Influenza A Virus, H1N1 Subtype.
Abstract
Ab-dependent cellular cytotoxicity, phagocytosis, and Ag presentation are key mechanisms of action of Abs arising in vaccine or naturally acquired immunity, as well of therapeutic mAbs. Cells expressing the low-affinity FcγRs (FcγRII or CD32 and FcγRIII or CD16) are activated for these functions when receptors are aggregated following the binding of IgG-opsonized targets. Despite the diversity of the Fc receptor proteins, IgG ligands, and potential responding cell types, the induction of all FcγR-mediated responses by opsonized targets requires the presentation of multiple Fc regions in close proximity to each other. We demonstrated that such "near-neighbor" Fc regions can be detected using defined recombinant soluble (rs) dimeric low-affinity ectodomains (rsFcγR) that have an absolute binding requirement for the simultaneous engagement of two IgG Fc regions. Like cell surface-expressed FcγRs, the binding of dimeric rsFcγR ectodomains to Ab immune complexes was affected by Ab subclass, presentation, opsonization density, Fc fucosylation, or mutation. The activation of an NK cell line and primary NK cells by human IgG-opsonized influenza A hemagglutinin correlated with dimeric rsFcγRIIIa binding activity but not with Ab titer. Furthermore, the dimeric rsFcγR binding assay sensitively detected greater Fc receptor activity to pandemic H1N1 hemagglutinin after the swine influenza pandemic of 2009 in pooled human polyclonal IgG. Thus these dimeric rsFcγR ectodomains are validated, defined probes that should prove valuable in measuring the immune-activating capacity of IgG Abs elicited by infection or vaccination or experimentally derived IgG and its variants.
DOI: 10.4049/jimmunol.1502551
PubMed: 27385782
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000517
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :000517
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000517
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :001B72
Links to Exploration step
pubmed:27385782Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Dimeric FcγR Ectodomains as Probes of the Fc Receptor Function of Anti-Influenza Virus IgG.</title>
<author><name sortKey="Wines, Bruce D" sort="Wines, Bruce D" uniqKey="Wines B" first="Bruce D" last="Wines">Bruce D. Wines</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria 3004, Australia; Department of Immunology, Monash University Central Clinical School, Melbourne, Victoria 3004, Australia; Department of Pathology, The University of Melbourne, Melbourne, Victoria 3052, Australia;</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria 3004, Australia; Department of Immunology, Monash University Central Clinical School, Melbourne, Victoria 3004, Australia; Department of Pathology, The University of Melbourne, Melbourne, Victoria 3052</wicri:regionArea>
<wicri:noRegion>Victoria 3052</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Vanderven, Hillary A" sort="Vanderven, Hillary A" uniqKey="Vanderven H" first="Hillary A" last="Vanderven">Hillary A. Vanderven</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria 3052, Australia;</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria 3052</wicri:regionArea>
<orgName type="university">Université de Melbourne</orgName>
<placeName><settlement type="city">Melbourne</settlement>
<region type="état">Victoria (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Esparon, Sandra E" sort="Esparon, Sandra E" uniqKey="Esparon S" first="Sandra E" last="Esparon">Sandra E. Esparon</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria 3004, Australia;</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria 3004</wicri:regionArea>
<wicri:noRegion>Victoria 3004</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kristensen, Anne B" sort="Kristensen, Anne B" uniqKey="Kristensen A" first="Anne B" last="Kristensen">Anne B. Kristensen</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria 3052, Australia;</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria 3052</wicri:regionArea>
<orgName type="university">Université de Melbourne</orgName>
<placeName><settlement type="city">Melbourne</settlement>
<region type="état">Victoria (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kent, Stephen J" sort="Kent, Stephen J" uniqKey="Kent S" first="Stephen J" last="Kent">Stephen J. Kent</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria 3052, Australia; Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology, The University of Melbourne, Parkville, Victoria 3052, Australia; and Melbourne Sexual Health Centre, Infectious Diseases Department, Alfred Health, Monash University Central Clinical School, Melbourne, Victoria 3004, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria 3052, Australia; Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology, The University of Melbourne, Parkville, Victoria 3052, Australia; and Melbourne Sexual Health Centre, Infectious Diseases Department, Alfred Health, Monash University Central Clinical School, Melbourne, Victoria 3004</wicri:regionArea>
<orgName type="university">Université de Melbourne</orgName>
<placeName><settlement type="city">Melbourne</settlement>
<region type="état">Victoria (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hogarth, P Mark" sort="Hogarth, P Mark" uniqKey="Hogarth P" first="P Mark" last="Hogarth">P Mark Hogarth</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria 3004, Australia; Department of Immunology, Monash University Central Clinical School, Melbourne, Victoria 3004, Australia; Department of Pathology, The University of Melbourne, Melbourne, Victoria 3052, Australia; mark.hogarth@burnet.edu.au.</nlm:affiliation>
<country wicri:rule="url">Australie</country>
<wicri:regionArea>Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria 3004, Australia; Department of Immunology, Monash University Central Clinical School, Melbourne, Victoria 3004, Australia; Department of Pathology, The University of Melbourne, Melbourne, Victoria 3052</wicri:regionArea>
<wicri:noRegion>Victoria 3052</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2016">2016</date>
<idno type="RBID">pubmed:27385782</idno>
<idno type="pmid">27385782</idno>
<idno type="doi">10.4049/jimmunol.1502551</idno>
<idno type="wicri:Area/PubMed/Corpus">000517</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000517</idno>
<idno type="wicri:Area/PubMed/Curation">000517</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000517</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000517</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000517</idno>
<idno type="wicri:Area/Ncbi/Merge">001B72</idno>
<idno type="wicri:Area/Ncbi/Curation">001B72</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Dimeric FcγR Ectodomains as Probes of the Fc Receptor Function of Anti-Influenza Virus IgG.</title>
<author><name sortKey="Wines, Bruce D" sort="Wines, Bruce D" uniqKey="Wines B" first="Bruce D" last="Wines">Bruce D. Wines</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria 3004, Australia; Department of Immunology, Monash University Central Clinical School, Melbourne, Victoria 3004, Australia; Department of Pathology, The University of Melbourne, Melbourne, Victoria 3052, Australia;</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria 3004, Australia; Department of Immunology, Monash University Central Clinical School, Melbourne, Victoria 3004, Australia; Department of Pathology, The University of Melbourne, Melbourne, Victoria 3052</wicri:regionArea>
<wicri:noRegion>Victoria 3052</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Vanderven, Hillary A" sort="Vanderven, Hillary A" uniqKey="Vanderven H" first="Hillary A" last="Vanderven">Hillary A. Vanderven</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria 3052, Australia;</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria 3052</wicri:regionArea>
<orgName type="university">Université de Melbourne</orgName>
<placeName><settlement type="city">Melbourne</settlement>
<region type="état">Victoria (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Esparon, Sandra E" sort="Esparon, Sandra E" uniqKey="Esparon S" first="Sandra E" last="Esparon">Sandra E. Esparon</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria 3004, Australia;</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria 3004</wicri:regionArea>
<wicri:noRegion>Victoria 3004</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kristensen, Anne B" sort="Kristensen, Anne B" uniqKey="Kristensen A" first="Anne B" last="Kristensen">Anne B. Kristensen</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria 3052, Australia;</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria 3052</wicri:regionArea>
<orgName type="university">Université de Melbourne</orgName>
<placeName><settlement type="city">Melbourne</settlement>
<region type="état">Victoria (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kent, Stephen J" sort="Kent, Stephen J" uniqKey="Kent S" first="Stephen J" last="Kent">Stephen J. Kent</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria 3052, Australia; Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology, The University of Melbourne, Parkville, Victoria 3052, Australia; and Melbourne Sexual Health Centre, Infectious Diseases Department, Alfred Health, Monash University Central Clinical School, Melbourne, Victoria 3004, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria 3052, Australia; Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology, The University of Melbourne, Parkville, Victoria 3052, Australia; and Melbourne Sexual Health Centre, Infectious Diseases Department, Alfred Health, Monash University Central Clinical School, Melbourne, Victoria 3004</wicri:regionArea>
<orgName type="university">Université de Melbourne</orgName>
<placeName><settlement type="city">Melbourne</settlement>
<region type="état">Victoria (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hogarth, P Mark" sort="Hogarth, P Mark" uniqKey="Hogarth P" first="P Mark" last="Hogarth">P Mark Hogarth</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria 3004, Australia; Department of Immunology, Monash University Central Clinical School, Melbourne, Victoria 3004, Australia; Department of Pathology, The University of Melbourne, Melbourne, Victoria 3052, Australia; mark.hogarth@burnet.edu.au.</nlm:affiliation>
<country wicri:rule="url">Australie</country>
<wicri:regionArea>Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria 3004, Australia; Department of Immunology, Monash University Central Clinical School, Melbourne, Victoria 3004, Australia; Department of Pathology, The University of Melbourne, Melbourne, Victoria 3052</wicri:regionArea>
<wicri:noRegion>Victoria 3052</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of immunology (Baltimore, Md. : 1950)</title>
<idno type="eISSN">1550-6606</idno>
<imprint><date when="2016" type="published">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (analysis)</term>
<term>Antibodies, Viral (immunology)</term>
<term>Antigen-Antibody Complex (immunology)</term>
<term>Humans</term>
<term>Immunoglobulin G (analysis)</term>
<term>Immunoglobulin G (immunology)</term>
<term>Immunologic Techniques (methods)</term>
<term>Influenza A Virus, H1N1 Subtype</term>
<term>Receptors, Fc (immunology)</term>
<term>Receptors, IgG (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (analyse)</term>
<term>Anticorps antiviraux (immunologie)</term>
<term>Complexe antigène-anticorps (immunologie)</term>
<term>Humains</term>
<term>Immunoglobuline G (analyse)</term>
<term>Immunoglobuline G (immunologie)</term>
<term>Récepteur Fc (immunologie)</term>
<term>Récepteurs du fragment Fc des IgG (immunologie)</term>
<term>Sous-type H1N1 du virus de la grippe A</term>
<term>Techniques immunologiques ()</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antibodies, Viral</term>
<term>Immunoglobulin G</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term>
<term>Antigen-Antibody Complex</term>
<term>Immunoglobulin G</term>
<term>Receptors, Fc</term>
<term>Receptors, IgG</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Immunoglobuline G</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Complexe antigène-anticorps</term>
<term>Immunoglobuline G</term>
<term>Récepteur Fc</term>
<term>Récepteurs du fragment Fc des IgG</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunologic Techniques</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Humans</term>
<term>Influenza A Virus, H1N1 Subtype</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Humains</term>
<term>Sous-type H1N1 du virus de la grippe A</term>
<term>Techniques immunologiques</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Ab-dependent cellular cytotoxicity, phagocytosis, and Ag presentation are key mechanisms of action of Abs arising in vaccine or naturally acquired immunity, as well of therapeutic mAbs. Cells expressing the low-affinity FcγRs (FcγRII or CD32 and FcγRIII or CD16) are activated for these functions when receptors are aggregated following the binding of IgG-opsonized targets. Despite the diversity of the Fc receptor proteins, IgG ligands, and potential responding cell types, the induction of all FcγR-mediated responses by opsonized targets requires the presentation of multiple Fc regions in close proximity to each other. We demonstrated that such "near-neighbor" Fc regions can be detected using defined recombinant soluble (rs) dimeric low-affinity ectodomains (rsFcγR) that have an absolute binding requirement for the simultaneous engagement of two IgG Fc regions. Like cell surface-expressed FcγRs, the binding of dimeric rsFcγR ectodomains to Ab immune complexes was affected by Ab subclass, presentation, opsonization density, Fc fucosylation, or mutation. The activation of an NK cell line and primary NK cells by human IgG-opsonized influenza A hemagglutinin correlated with dimeric rsFcγRIIIa binding activity but not with Ab titer. Furthermore, the dimeric rsFcγR binding assay sensitively detected greater Fc receptor activity to pandemic H1N1 hemagglutinin after the swine influenza pandemic of 2009 in pooled human polyclonal IgG. Thus these dimeric rsFcγR ectodomains are validated, defined probes that should prove valuable in measuring the immune-activating capacity of IgG Abs elicited by infection or vaccination or experimentally derived IgG and its variants.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/PandemieGrippaleV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001B72 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 001B72 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= PandemieGrippaleV1 |flux= Ncbi |étape= Curation |type= RBID |clé= pubmed:27385782 |texte= Dimeric FcγR Ectodomains as Probes of the Fc Receptor Function of Anti-Influenza Virus IgG. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:27385782" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \ | NlmPubMed2Wicri -a PandemieGrippaleV1
![]() | This area was generated with Dilib version V0.6.34. | ![]() |