1918 Influenza Receptor Binding Domain Variants Bind and Replicate in Primary Human Airway Cells Regardless of Receptor Specificity
Identifieur interne : 001B52 ( Ncbi/Checkpoint ); précédent : 001B51; suivant : 001B531918 Influenza Receptor Binding Domain Variants Bind and Replicate in Primary Human Airway Cells Regardless of Receptor Specificity
Auteurs : A. Sally Davis [États-Unis] ; Daniel S. Chertow [États-Unis] ; Jason Kindrachuk [États-Unis] ; Li Qi [États-Unis] ; Louis M. Schwartzman [États-Unis] ; Jon Suzich [États-Unis] ; Sara Alsaaty [États-Unis] ; Carolea Logun [États-Unis] ; James H. Shelhamer [États-Unis] ; Jeffery K. Taubenberger [États-Unis]Source :
- Virology [ 0042-6822 ] ; 2016.
Descripteurs français
- KwdFr :
- Acide N-acétyl-neuraminique (métabolisme), Attachement viral, Bronches (cytologie), Cellules épithéliales (virologie), Pandémie de grippe de 1918-1919, Réaction de polymérisation en chaine en temps réel, Récepteurs viraux (métabolisme), Réplication virale, Sites de fixation, Sous-type H1N1 du virus de la grippe A (métabolisme), Trachée (cytologie).
- MESH :
- cytologie : Bronches, Trachée.
- métabolisme : Acide N-acétyl-neuraminique, Récepteurs viraux, Sous-type H1N1 du virus de la grippe A.
- virologie : Cellules épithéliales.
- Attachement viral, Pandémie de grippe de 1918-1919, Réaction de polymérisation en chaine en temps réel, Réplication virale, Sites de fixation.
English descriptors
- KwdEn :
- Binding Sites, Bronchi (cytology), Epithelial Cells (virology), Influenza A Virus, H1N1 Subtype (metabolism), Influenza Pandemic, 1918-1919, N-Acetylneuraminic Acid (metabolism), Real-Time Polymerase Chain Reaction, Receptors, Virus (metabolism), Trachea (cytology), Virus Attachment, Virus Replication.
- MESH :
- chemical , metabolism : N-Acetylneuraminic Acid, Receptors, Virus.
- cytology : Bronchi, Trachea.
- metabolism : Influenza A Virus, H1N1 Subtype.
- virology : Epithelial Cells.
- Binding Sites, Influenza Pandemic, 1918-1919, Real-Time Polymerase Chain Reaction, Virus Attachment, Virus Replication.
Abstract
The 1918 influenza pandemic caused ~50 million deaths. Many questions remain regarding the origin, pathogenicity, and mechanisms of human adaptation of this virus. Avian-adapted influenza A viruses preferentially bind α2,3-linked sialic acids (Sia) while human-adapted viruses preferentially bind α2,6-linked Sia. A change in Sia preference from α2,3 to α2,6 is thought to be a requirement for human adaptation of avian influenza viruses. Autopsy data from 1918 cases, however, suggest that factors other than Sia preference played a role in viral binding and entry to human airway cells. Here, we evaluated binding and entry of five 1918 influenza receptor binding domain variants in a primary human airway cell model along with control avian and human influenza viruses. We observed that all five variants bound and entered cells efficiently and that Sia preference did not predict entry of influenza A virus to primary human airway cells evaluated in this model.
Url:
DOI: 10.1016/j.virol.2016.03.025
PubMed: 27062579
PubMed Central: 4949484
Affiliations:
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Schwartzman</name><affiliation wicri:level="2"><nlm:aff id="A1">Viral Pathogenesis and Evolution Section, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Viral Pathogenesis and Evolution Section, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Suzich, Jon" sort="Suzich, Jon" uniqKey="Suzich J" first="Jon" last="Suzich">Jon Suzich</name><affiliation wicri:level="2"><nlm:aff id="A1">Viral Pathogenesis and Evolution Section, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Viral Pathogenesis and Evolution Section, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="A3">Critical Care Medicine Department, Clinical Center, NIH, Bethesda, MD</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Critical Care Medicine Department, Clinical Center, NIH, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Alsaaty, Sara" sort="Alsaaty, Sara" uniqKey="Alsaaty S" first="Sara" last="Alsaaty">Sara Alsaaty</name><affiliation wicri:level="2"><nlm:aff id="A3">Critical Care Medicine Department, Clinical Center, NIH, Bethesda, MD</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Critical Care Medicine Department, Clinical Center, NIH, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Logun, Carolea" sort="Logun, Carolea" uniqKey="Logun C" first="Carolea" last="Logun">Carolea Logun</name><affiliation wicri:level="2"><nlm:aff id="A3">Critical Care Medicine Department, Clinical Center, NIH, Bethesda, MD</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Critical Care Medicine Department, Clinical Center, NIH, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Shelhamer, James H" sort="Shelhamer, James H" uniqKey="Shelhamer J" first="James H." last="Shelhamer">James H. 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Sally" last="Davis">A. Sally Davis</name><affiliation wicri:level="2"><nlm:aff id="A1">Viral Pathogenesis and Evolution Section, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Viral Pathogenesis and Evolution Section, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="A2">Diagnostic Medicine and Pathobiology, Kansas State University College of Veterinary Medicine, Manhattan, KS</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Kansas</region></placeName><wicri:cityArea>Diagnostic Medicine and Pathobiology, Kansas State University College of Veterinary Medicine, Manhattan</wicri:cityArea></affiliation></author><author><name sortKey="Chertow, Daniel S" sort="Chertow, Daniel S" uniqKey="Chertow D" first="Daniel S." last="Chertow">Daniel S. Chertow</name><affiliation wicri:level="2"><nlm:aff id="A1">Viral Pathogenesis and Evolution Section, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Viral Pathogenesis and Evolution Section, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="A3">Critical Care Medicine Department, Clinical Center, NIH, Bethesda, MD</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Critical Care Medicine Department, Clinical Center, NIH, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Kindrachuk, Jason" sort="Kindrachuk, Jason" uniqKey="Kindrachuk J" first="Jason" last="Kindrachuk">Jason Kindrachuk</name><affiliation wicri:level="2"><nlm:aff id="A1">Viral Pathogenesis and Evolution Section, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Viral Pathogenesis and Evolution Section, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="A3">Critical Care Medicine Department, Clinical Center, NIH, Bethesda, MD</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Critical Care Medicine Department, Clinical Center, NIH, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Qi, Li" sort="Qi, Li" uniqKey="Qi L" first="Li" last="Qi">Li Qi</name><affiliation wicri:level="2"><nlm:aff id="A1">Viral Pathogenesis and Evolution Section, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Viral Pathogenesis and Evolution Section, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Schwartzman, Louis M" sort="Schwartzman, Louis M" uniqKey="Schwartzman L" first="Louis M." last="Schwartzman">Louis M. Schwartzman</name><affiliation wicri:level="2"><nlm:aff id="A1">Viral Pathogenesis and Evolution Section, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Viral Pathogenesis and Evolution Section, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Suzich, Jon" sort="Suzich, Jon" uniqKey="Suzich J" first="Jon" last="Suzich">Jon Suzich</name><affiliation wicri:level="2"><nlm:aff id="A1">Viral Pathogenesis and Evolution Section, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Viral Pathogenesis and Evolution Section, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="A3">Critical Care Medicine Department, Clinical Center, NIH, Bethesda, MD</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Critical Care Medicine Department, Clinical Center, NIH, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Alsaaty, Sara" sort="Alsaaty, Sara" uniqKey="Alsaaty S" first="Sara" last="Alsaaty">Sara Alsaaty</name><affiliation wicri:level="2"><nlm:aff id="A3">Critical Care Medicine Department, Clinical Center, NIH, Bethesda, MD</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Critical Care Medicine Department, Clinical Center, NIH, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Logun, Carolea" sort="Logun, Carolea" uniqKey="Logun C" first="Carolea" last="Logun">Carolea Logun</name><affiliation wicri:level="2"><nlm:aff id="A3">Critical Care Medicine Department, Clinical Center, NIH, Bethesda, MD</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Critical Care Medicine Department, Clinical Center, NIH, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Shelhamer, James H" sort="Shelhamer, James H" uniqKey="Shelhamer J" first="James H." last="Shelhamer">James H. Shelhamer</name><affiliation wicri:level="2"><nlm:aff id="A3">Critical Care Medicine Department, Clinical Center, NIH, Bethesda, MD</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Critical Care Medicine Department, Clinical Center, NIH, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Taubenberger, Jeffery K" sort="Taubenberger, Jeffery K" uniqKey="Taubenberger J" first="Jeffery K." last="Taubenberger">Jeffery K. Taubenberger</name><affiliation wicri:level="2"><nlm:aff id="A1">Viral Pathogenesis and Evolution Section, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Viral Pathogenesis and Evolution Section, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda</wicri:cityArea></affiliation></author></analytic><series><title level="j">Virology</title><idno type="ISSN">0042-6822</idno><idno type="eISSN">1096-0341</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Binding Sites</term><term>Bronchi (cytology)</term><term>Epithelial Cells (virology)</term><term>Influenza A Virus, H1N1 Subtype (metabolism)</term><term>Influenza Pandemic, 1918-1919</term><term>N-Acetylneuraminic Acid (metabolism)</term><term>Real-Time Polymerase Chain Reaction</term><term>Receptors, Virus (metabolism)</term><term>Trachea (cytology)</term><term>Virus Attachment</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide N-acétyl-neuraminique (métabolisme)</term><term>Attachement viral</term><term>Bronches (cytologie)</term><term>Cellules épithéliales (virologie)</term><term>Pandémie de grippe de 1918-1919</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Récepteurs viraux (métabolisme)</term><term>Réplication virale</term><term>Sites de fixation</term><term>Sous-type H1N1 du virus de la grippe A (métabolisme)</term><term>Trachée (cytologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>N-Acetylneuraminic Acid</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Bronches</term><term>Trachée</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Bronchi</term><term>Trachea</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acide N-acétyl-neuraminique</term><term>Récepteurs viraux</term><term>Sous-type H1N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules épithéliales</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Epithelial Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Binding Sites</term><term>Influenza Pandemic, 1918-1919</term><term>Real-Time Polymerase Chain Reaction</term><term>Virus Attachment</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Attachement viral</term><term>Pandémie de grippe de 1918-1919</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Réplication virale</term><term>Sites de fixation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P2">The 1918 influenza pandemic caused ~50 million deaths. Many questions remain regarding the origin, pathogenicity, and mechanisms of human adaptation of this virus. Avian-adapted influenza A viruses preferentially bind α2,3-linked sialic acids (Sia) while human-adapted viruses preferentially bind α2,6-linked Sia. A change in Sia preference from α2,3 to α2,6 is thought to be a requirement for human adaptation of avian influenza viruses. Autopsy data from 1918 cases, however, suggest that factors other than Sia preference played a role in viral binding and entry to human airway cells. Here, we evaluated binding and entry of five 1918 influenza receptor binding domain variants in a primary human airway cell model along with control avian and human influenza viruses. We observed that all five variants bound and entered cells efficiently and that Sia preference did not predict entry of influenza A virus to primary human airway cells evaluated in this model.</p></div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Kansas</li><li>Maryland</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Davis, A Sally" sort="Davis, A Sally" uniqKey="Davis A" first="A. Sally" last="Davis">A. Sally Davis</name></region><name sortKey="Alsaaty, Sara" sort="Alsaaty, Sara" uniqKey="Alsaaty S" first="Sara" last="Alsaaty">Sara Alsaaty</name><name sortKey="Chertow, Daniel S" sort="Chertow, Daniel S" uniqKey="Chertow D" first="Daniel S." last="Chertow">Daniel S. Chertow</name><name sortKey="Chertow, Daniel S" sort="Chertow, Daniel S" uniqKey="Chertow D" first="Daniel S." last="Chertow">Daniel S. Chertow</name><name sortKey="Davis, A Sally" sort="Davis, A Sally" uniqKey="Davis A" first="A. Sally" last="Davis">A. Sally Davis</name><name sortKey="Kindrachuk, Jason" sort="Kindrachuk, Jason" uniqKey="Kindrachuk J" first="Jason" last="Kindrachuk">Jason Kindrachuk</name><name sortKey="Kindrachuk, Jason" sort="Kindrachuk, Jason" uniqKey="Kindrachuk J" first="Jason" last="Kindrachuk">Jason Kindrachuk</name><name sortKey="Logun, Carolea" sort="Logun, Carolea" uniqKey="Logun C" first="Carolea" last="Logun">Carolea Logun</name><name sortKey="Qi, Li" sort="Qi, Li" uniqKey="Qi L" first="Li" last="Qi">Li Qi</name><name sortKey="Schwartzman, Louis M" sort="Schwartzman, Louis M" uniqKey="Schwartzman L" first="Louis M." last="Schwartzman">Louis M. Schwartzman</name><name sortKey="Shelhamer, James H" sort="Shelhamer, James H" uniqKey="Shelhamer J" first="James H." last="Shelhamer">James H. Shelhamer</name><name sortKey="Suzich, Jon" sort="Suzich, Jon" uniqKey="Suzich J" first="Jon" last="Suzich">Jon Suzich</name><name sortKey="Suzich, Jon" sort="Suzich, Jon" uniqKey="Suzich J" first="Jon" last="Suzich">Jon Suzich</name><name sortKey="Taubenberger, Jeffery K" sort="Taubenberger, Jeffery K" uniqKey="Taubenberger J" first="Jeffery K." last="Taubenberger">Jeffery K. Taubenberger</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|flux= Ncbi
|étape= Checkpoint
|type= RBID
|clé= PMC:4949484
|texte= 1918 Influenza Receptor Binding Domain Variants Bind and Replicate in Primary Human Airway Cells Regardless of Receptor Specificity
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:27062579" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a PandemieGrippaleV1
| This area was generated with Dilib version V0.6.34. |  |