PandemieGrippaleV1, Main, Merge, bibRecord, 003380

Detection of swine-origin influenza A (H1N1) viruses using a localized surface plasmon coupled fluorescence fiber-optic biosensor

Identifieur interne : 003380 ( Main/Merge ); précédent : 003379; suivant : 003381

Detection of swine-origin influenza A (H1N1) viruses using a localized surface plasmon coupled fluorescence fiber-optic biosensor

Auteurs : Ying-Feng Chang [Taïwan] ; Sheng-Fan Wang [Taïwan] ; Jason C. Huang [Taïwan] ; Li-Chen Su [Taïwan] ; Ling Yao [Taïwan] ; Ying-Chang Li [Taïwan] ; Suh-Chin Wu [Taïwan] ; Yi-Ming A. Chen [Taïwan] ; Jo-Ping Hsieh [Taïwan] ; Chien Chou [Taïwan]

Source :

Biosensors & Bioelectronics [ 0956-5663 ] ; 2010.

RBID : PMC:7126842

Descripteurs français

English descriptors

KwdEn :
- Animals, Antibodies, Viral, Base Sequence, DNA Primers (genetics), Enzyme-Linked Immunosorbent Assay, Fluorescence, Hemagglutinin Glycoproteins, Influenza Virus (analysis), Hemagglutinin Glycoproteins, Influenza Virus (genetics), Hemagglutinin Glycoproteins, Influenza Virus (immunology), Humans, Influenza A Virus, H1N1 Subtype (genetics), Influenza A Virus, H1N1 Subtype (immunology), Influenza A Virus, H1N1 Subtype (isolation & purification), Influenza, Human (diagnosis), Influenza, Human (epidemiology), Influenza, Human (virology), Optical Fibers, Pandemics, Reassortant Viruses (genetics), Reassortant Viruses (isolation & purification), Recombinant Proteins (analysis), Recombinant Proteins (genetics), Recombinant Proteins (immunology), Reverse Transcriptase Polymerase Chain Reaction, Sensitivity and Specificity, Surface Plasmon Resonance (instrumentation), Surface Plasmon Resonance (methods), Surface Plasmon Resonance (statistics & numerical data), Swine (virology).
MESH :
- chemical , analysis : Hemagglutinin Glycoproteins, Influenza Virus, Recombinant Proteins.
- chemical , genetics : DNA Primers, Hemagglutinin Glycoproteins, Influenza Virus, Recombinant Proteins.
- chemical , immunology : Hemagglutinin Glycoproteins, Influenza Virus, Recombinant Proteins.
- chemical : Antibodies, Viral.
- diagnosis : Influenza, Human.
- epidemiology : Influenza, Human.
- genetics : Influenza A Virus, H1N1 Subtype, Reassortant Viruses.
- immunology : Influenza A Virus, H1N1 Subtype.
- instrumentation : Surface Plasmon Resonance.
- isolation & purification : Influenza A Virus, H1N1 Subtype, Reassortant Viruses.
- methods : Surface Plasmon Resonance.
- statistics & numerical data : Surface Plasmon Resonance.
- virology : Influenza, Human, Swine.
- Animals, Base Sequence, Enzyme-Linked Immunosorbent Assay, Fluorescence, Humans, Optical Fibers, Pandemics, Reverse Transcriptase Polymerase Chain Reaction, Sensitivity and Specificity.

Abstract

Swine-origin influenza A (H1N1) virus (S-OIV) was identified as a new reassortant strain of influenza A virus in April 2009 and led to an influenza pandemic. Accurate and timely diagnoses are crucial for the control of influenza disease. We developed a localized surface plasmon coupled fluorescence fiber-optic biosensor (LSPCF-FOB) which combines a sandwich immunoassay with the LSP technique using antibodies against the hemagglutinin (HA) proteins of S-OIVs. The detection limit of the LSPCF-FOB for recombinant S-OIV H1 protein detection was estimated at 13.9 pg/mL, which is 10³-fold better than that of conventional capture ELISA when using the same capture antibodies. For clinical S-OIV isolates measurement, meanwhile, the detection limit of the LSPCF-FOB platform was calculated to be 8.25 × 10⁴ copies/mL, compared with 2.06 × 10⁶ copies/mL using conventional capture ELISA. Furthermore, in comparison with the influenza A/B rapid test, the detection limit of the LSPCF-FOB for S-OIV was almost 50-fold in PBS solution and 25-fold lower in mimic solution, which used nasal mucosa from healthy donors as the diluent. The findings of this study therefore indicate that the high detection sensitivity and specificity of the LSPCF-FOB make it a potentially effective diagnostic tool for clinical S-OIV infection and this technique has the potential to be applied to the development of other clinical microbe detection platforms.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126842

DOI: 10.1016/j.bios.2010.08.060
PubMed: 20855191
PubMed Central: 7126842

Links to Exploration step

PMC:7126842

Le document en format XML

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<term>Antibodies, Viral</term>
<term>Base Sequence</term>
<term>DNA Primers (genetics)</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Fluorescence</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (analysis)</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (genetics)</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (immunology)</term>
<term>Humans</term>
<term>Influenza A Virus, H1N1 Subtype (genetics)</term>
<term>Influenza A Virus, H1N1 Subtype (immunology)</term>
<term>Influenza A Virus, H1N1 Subtype (isolation & purification)</term>
<term>Influenza, Human (diagnosis)</term>
<term>Influenza, Human (epidemiology)</term>
<term>Influenza, Human (virology)</term>
<term>Optical Fibers</term>
<term>Pandemics</term>
<term>Reassortant Viruses (genetics)</term>
<term>Reassortant Viruses (isolation & purification)</term>
<term>Recombinant Proteins (analysis)</term>
<term>Recombinant Proteins (genetics)</term>
<term>Recombinant Proteins (immunology)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>Sensitivity and Specificity</term>
<term>Surface Plasmon Resonance (instrumentation)</term>
<term>Surface Plasmon Resonance (methods)</term>
<term>Surface Plasmon Resonance (statistics & numerical data)</term>
<term>Swine (virology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN (génétique)</term>
<term>Animaux</term>
<term>Anticorps antiviraux</term>
<term>Fibres optiques</term>
<term>Fluorescence</term>
<term>Glycoprotéine hémagglutinine du virus influenza (analyse)</term>
<term>Glycoprotéine hémagglutinine du virus influenza (génétique)</term>
<term>Glycoprotéine hémagglutinine du virus influenza (immunologie)</term>
<term>Grippe humaine (diagnostic)</term>
<term>Grippe humaine (virologie)</term>
<term>Grippe humaine (épidémiologie)</term>
<term>Humains</term>
<term>Pandémies</term>
<term>Protéines recombinantes (analyse)</term>
<term>Protéines recombinantes (génétique)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>RT-PCR</term>
<term>Résonance plasmonique de surface ()</term>
<term>Résonance plasmonique de surface (instrumentation)</term>
<term>Sensibilité et spécificité</term>
<term>Sous-type H1N1 du virus de la grippe A (génétique)</term>
<term>Sous-type H1N1 du virus de la grippe A (immunologie)</term>
<term>Sous-type H1N1 du virus de la grippe A (isolement et purification)</term>
<term>Suidae (virologie)</term>
<term>Séquence nucléotidique</term>
<term>Test ELISA</term>
<term>Virus recombinants (génétique)</term>
<term>Virus recombinants (isolement et purification)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Hemagglutinin Glycoproteins, Influenza Virus</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA Primers</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Hemagglutinin Glycoproteins, Influenza Virus</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Glycoprotéine hémagglutinine du virus influenza</term>
<term>Protéines recombinantes</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe humaine</term>
</keywords>
<keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Influenza, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term>
<term>Reassortant Viruses</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Amorces ADN</term>
<term>Glycoprotéine hémagglutinine du virus influenza</term>
<term>Protéines recombinantes</term>
<term>Sous-type H1N1 du virus de la grippe A</term>
<term>Virus recombinants</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéine hémagglutinine du virus influenza</term>
<term>Protéines recombinantes</term>
<term>Sous-type H1N1 du virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term>
</keywords>
<keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Surface Plasmon Resonance</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term>
<term>Reassortant Viruses</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term>
<term>Virus recombinants</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Surface Plasmon Resonance</term>
</keywords>
<keywords scheme="MESH" qualifier="statistics & numerical data" xml:lang="en"><term>Surface Plasmon Resonance</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term>
<term>Suidae</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza, Human</term>
<term>Swine</term>
</keywords>
<keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Grippe humaine</term>
<term>Résonance plasmonique de surface</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Base Sequence</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Fluorescence</term>
<term>Humans</term>
<term>Optical Fibers</term>
<term>Pandemics</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>Sensitivity and Specificity</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Anticorps antiviraux</term>
<term>Fibres optiques</term>
<term>Fluorescence</term>
<term>Humains</term>
<term>Pandémies</term>
<term>RT-PCR</term>
<term>Résonance plasmonique de surface</term>
<term>Sensibilité et spécificité</term>
<term>Séquence nucléotidique</term>
<term>Test ELISA</term>
</keywords>
</textClass>
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</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Swine-origin influenza A (H1N1) virus (S-OIV) was identified as a new reassortant strain of influenza A virus in April 2009 and led to an influenza pandemic. Accurate and timely diagnoses are crucial for the control of influenza disease. We developed a localized surface plasmon coupled fluorescence fiber-optic biosensor (LSPCF-FOB) which combines a sandwich immunoassay with the LSP technique using antibodies against the hemagglutinin (HA) proteins of S-OIVs. The detection limit of the LSPCF-FOB for recombinant S-OIV H1 protein detection was estimated at 13.9 pg/mL, which is 10<sup>3</sup>
-fold better than that of conventional capture ELISA when using the same capture antibodies. For clinical S-OIV isolates measurement, meanwhile, the detection limit of the LSPCF-FOB platform was calculated to be 8.25 × 10<sup>4</sup>
 copies/mL, compared with 2.06 × 10<sup>6</sup>
 copies/mL using conventional capture ELISA. Furthermore, in comparison with the influenza A/B rapid test, the detection limit of the LSPCF-FOB for S-OIV was almost 50-fold in PBS solution and 25-fold lower in mimic solution, which used nasal mucosa from healthy donors as the diluent. The findings of this study therefore indicate that the high detection sensitivity and specificity of the LSPCF-FOB make it a potentially effective diagnostic tool for clinical S-OIV infection and this technique has the potential to be applied to the development of other clinical microbe detection platforms.</p>
</div>
</front>
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Serveur d'exploration sur les pandémies grippales

Detection of swine-origin influenza A (H1N1) viruses using a localized surface plasmon coupled fluorescence fiber-optic biosensor

Detection of swine-origin influenza A (H1N1) viruses using a localized surface plasmon coupled fluorescence fiber-optic biosensor

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