Bordetella pertussis monophosphoryl lipid A as adjuvant for inactivated split virion influenza vaccine in mice.
Identifieur interne : 003E25 ( Main/Exploration ); précédent : 003E24; suivant : 003E26Bordetella pertussis monophosphoryl lipid A as adjuvant for inactivated split virion influenza vaccine in mice.
Auteurs : Wagner Quintilio [Brésil] ; Flavia S. Kubrusly ; Dmitri Iourtov ; Cosue Miyaki ; Maria Aparecida Sakauchi ; Fernanda Lúcio ; Sandra De Cássia Dias ; Celia S. Takata ; Eliane N. Miyaji ; Hisako G. Higashi ; Luciana C C. Leite ; Isaias RawSource :
- Vaccine [ 1873-2518 ] ; 2009.
Descripteurs français
- KwdFr :
- Adjuvants immunologiques (isolement et purification), Adjuvants immunologiques (pharmacologie), Animaux, Anticorps antiviraux (sang), Bordetella pertussis (), Hydroxyde d'aluminium (pharmacologie), Immunoglobuline G (sang), Interféron gamma (métabolisme), Interleukine-4 (métabolisme), Lipide A (analogues et dérivés), Lipide A (isolement et purification), Lipide A (pharmacologie), Souris, Souris de lignée BALB C, Tests d'inhibition de l'hémagglutination, Vaccins antigrippaux (immunologie), Vaccins sous-unitaires (immunologie).
- MESH :
- analogues et dérivés : Lipide A.
- immunologie : Vaccins antigrippaux, Vaccins sous-unitaires.
- isolement et purification : Adjuvants immunologiques, Lipide A.
- métabolisme : Interféron gamma, Interleukine-4.
- pharmacologie : Adjuvants immunologiques, Hydroxyde d'aluminium, Lipide A.
- sang : Anticorps antiviraux, Immunoglobuline G.
- Animaux, Bordetella pertussis, Souris, Souris de lignée BALB C, Tests d'inhibition de l'hémagglutination.
English descriptors
- KwdEn :
- Adjuvants, Immunologic (isolation & purification), Adjuvants, Immunologic (pharmacology), Aluminum Hydroxide (pharmacology), Animals, Antibodies, Viral (blood), Bordetella pertussis (chemistry), Hemagglutination Inhibition Tests, Immunoglobulin G (blood), Influenza Vaccines (immunology), Interferon-gamma (metabolism), Interleukin-4 (metabolism), Lipid A (analogs & derivatives), Lipid A (isolation & purification), Lipid A (pharmacology), Mice, Mice, Inbred BALB C, Vaccines, Subunit (immunology).
- MESH :
- chemical , analogs & derivatives : Lipid A.
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- chemical , immunology : Influenza Vaccines, Vaccines, Subunit.
- chemical , isolation & purification : Adjuvants, Immunologic, Lipid A.
- chemical , metabolism : Interferon-gamma, Interleukin-4.
- chemical , pharmacology : Adjuvants, Immunologic, Aluminum Hydroxide, Lipid A.
- chemistry : Bordetella pertussis.
- Animals, Hemagglutination Inhibition Tests, Mice, Mice, Inbred BALB C.
Abstract
The world production capacity of influenza vaccines is a concern in face of the potential influenza pandemic. The use of adjuvants could increase several fold the current installed production capacity. Bordetella pertussis monophosphyl lipid A (MPLA) was produced by acid hydrolysis of LPS, obtained as a by-product of its removal from cellular pertussis vaccine, generating a product with 4 side chains. We have investigated different formulations including MPLA alone or combined with Al(OH)(3) as adjuvants for an inactivated split virion influenza vaccine. Our results demonstrate that MPLA at concentrations as low as 0.01 microg per dose of vaccine is effective, even with a 4-fold reduction of the regular vaccine dose, as measured by the induction of protective hemagglutination inhibition (HAI) titers. Al(OH)(3) can be combined with 0.01-10 microg MPLA, inducing even higher immune responses. Al(OH)(3) caused a drift of the immune response induced by the vaccine towards a Th2 profile, as evaluated by an increase in the IgG1:IgG2a ratio, while MPLA showed a more balanced response. Moreover, the use of MPLA and Al(OH)(3) combination led to the induction of the highest IgG levels together with the secretion of both IFN-gamma and IL-4. Although cell-mediated immune responses have not been usually taken into account for influenza vaccine formulations, they may be relevant for the induction of cross-protection as well as immunological memory for both inter-pandemic and pandemic influenza vaccines. Our results indicate that a more favorable profile of both humoral and cell-mediated immune responses may be obtained using the MPLA/Al(OH)(3) formulation.
DOI: 10.1016/j.vaccine.2009.04.047
PubMed: 19393709
Affiliations:
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Le document en format XML
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Kubrusly</name></author><author><name sortKey="Iourtov, Dmitri" sort="Iourtov, Dmitri" uniqKey="Iourtov D" first="Dmitri" last="Iourtov">Dmitri Iourtov</name></author><author><name sortKey="Miyaki, Cosue" sort="Miyaki, Cosue" uniqKey="Miyaki C" first="Cosue" last="Miyaki">Cosue Miyaki</name></author><author><name sortKey="Sakauchi, Maria Aparecida" sort="Sakauchi, Maria Aparecida" uniqKey="Sakauchi M" first="Maria Aparecida" last="Sakauchi">Maria Aparecida Sakauchi</name></author><author><name sortKey="Lucio, Fernanda" sort="Lucio, Fernanda" uniqKey="Lucio F" first="Fernanda" last="Lúcio">Fernanda Lúcio</name></author><author><name sortKey="Dias, Sandra De Cassia" sort="Dias, Sandra De Cassia" uniqKey="Dias S" first="Sandra De Cássia" last="Dias">Sandra De Cássia Dias</name></author><author><name sortKey="Takata, Celia S" sort="Takata, Celia S" uniqKey="Takata C" first="Celia S" last="Takata">Celia S. 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The use of adjuvants could increase several fold the current installed production capacity. Bordetella pertussis monophosphyl lipid A (MPLA) was produced by acid hydrolysis of LPS, obtained as a by-product of its removal from cellular pertussis vaccine, generating a product with 4 side chains. We have investigated different formulations including MPLA alone or combined with Al(OH)(3) as adjuvants for an inactivated split virion influenza vaccine. Our results demonstrate that MPLA at concentrations as low as 0.01 microg per dose of vaccine is effective, even with a 4-fold reduction of the regular vaccine dose, as measured by the induction of protective hemagglutination inhibition (HAI) titers. Al(OH)(3) can be combined with 0.01-10 microg MPLA, inducing even higher immune responses. Al(OH)(3) caused a drift of the immune response induced by the vaccine towards a Th2 profile, as evaluated by an increase in the IgG1:IgG2a ratio, while MPLA showed a more balanced response. Moreover, the use of MPLA and Al(OH)(3) combination led to the induction of the highest IgG levels together with the secretion of both IFN-gamma and IL-4. Although cell-mediated immune responses have not been usually taken into account for influenza vaccine formulations, they may be relevant for the induction of cross-protection as well as immunological memory for both inter-pandemic and pandemic influenza vaccines. Our results indicate that a more favorable profile of both humoral and cell-mediated immune responses may be obtained using the MPLA/Al(OH)(3) formulation.</div></front></TEI><affiliations><list><country><li>Brésil</li></country><region><li>État de São Paulo</li></region></list><tree><noCountry><name sortKey="Dias, Sandra De Cassia" sort="Dias, Sandra De Cassia" uniqKey="Dias S" first="Sandra De Cássia" last="Dias">Sandra De Cássia Dias</name><name sortKey="Higashi, Hisako G" sort="Higashi, Hisako G" uniqKey="Higashi H" first="Hisako G" last="Higashi">Hisako G. Higashi</name><name sortKey="Iourtov, Dmitri" sort="Iourtov, Dmitri" uniqKey="Iourtov D" first="Dmitri" last="Iourtov">Dmitri Iourtov</name><name sortKey="Kubrusly, Flavia S" sort="Kubrusly, Flavia S" uniqKey="Kubrusly F" first="Flavia S" last="Kubrusly">Flavia S. Kubrusly</name><name sortKey="Leite, Luciana C C" sort="Leite, Luciana C C" uniqKey="Leite L" first="Luciana C C" last="Leite">Luciana C C. Leite</name><name sortKey="Lucio, Fernanda" sort="Lucio, Fernanda" uniqKey="Lucio F" first="Fernanda" last="Lúcio">Fernanda Lúcio</name><name sortKey="Miyaji, Eliane N" sort="Miyaji, Eliane N" uniqKey="Miyaji E" first="Eliane N" last="Miyaji">Eliane N. Miyaji</name><name sortKey="Miyaki, Cosue" sort="Miyaki, Cosue" uniqKey="Miyaki C" first="Cosue" last="Miyaki">Cosue Miyaki</name><name sortKey="Raw, Isaias" sort="Raw, Isaias" uniqKey="Raw I" first="Isaias" last="Raw">Isaias Raw</name><name sortKey="Sakauchi, Maria Aparecida" sort="Sakauchi, Maria Aparecida" uniqKey="Sakauchi M" first="Maria Aparecida" last="Sakauchi">Maria Aparecida Sakauchi</name><name sortKey="Takata, Celia S" sort="Takata, Celia S" uniqKey="Takata C" first="Celia S" last="Takata">Celia S. Takata</name></noCountry><country name="Brésil"><region name="État de São Paulo"><name sortKey="Quintilio, Wagner" sort="Quintilio, Wagner" uniqKey="Quintilio W" first="Wagner" last="Quintilio">Wagner Quintilio</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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