Diagnosis of Influenza from Respiratory Autopsy Tissues
Identifieur interne : 002545 ( Main/Exploration ); précédent : 002544; suivant : 002546Diagnosis of Influenza from Respiratory Autopsy Tissues
Auteurs : Amy M. Denison [Géorgie (pays)] ; Dianna M. Blau [Géorgie (pays)] ; Heather A. Jost [Géorgie (pays)] ; Tara Jones [Géorgie (pays)] ; Dominique Rollin [Géorgie (pays)] ; Rongbao Gao [République populaire de Chine] ; Lindy Liu [Géorgie (pays)] ; Julu Bhatnagar [Géorgie (pays)] ; Marlene Deleon-Carnes [Géorgie (pays)] ; Wun-Ju Shieh [Géorgie (pays)] ; Christopher D. Paddock [Géorgie (pays)] ; Clifton Drew [Géorgie (pays)] ; Patricia Adem [Géorgie (pays)] ; Shannon L. Emery [Géorgie (pays)] ; Bo Shu [Géorgie (pays)] ; Kai-Hui Wu [Géorgie (pays)] ; Brigid Batten [Géorgie (pays)] ; Patricia W. Greer [Géorgie (pays)] ; Chalanda S. Smith [Géorgie (pays)] ; Jeanine Bartlett [Géorgie (pays)] ; Jeltley L. Montague [Géorgie (pays)] ; Mitesh Patel [Géorgie (pays)] ; Xiyan Xu [Géorgie (pays)] ; Stephen Lindstrom [Géorgie (pays)] ; Alexander I. Klimov [Géorgie (pays)] ; Sherif R. Zaki [Géorgie (pays)]Source :
- The Journal of Molecular Diagnostics : JMD [ 1525-1578 ] ; 2011.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ARN viral.
- anatomie et histologie : Appareil respiratoire.
- diagnostic : Grippe humaine.
- génétique : Virus de la grippe A, Virus influenza B.
- virologie : Appareil respiratoire, Grippe humaine.
- Autopsie, Humains, Immunohistochimie, RT-PCR.
English descriptors
- KwdEn :
- Autopsy, Humans, Immunohistochemistry, Influenza A virus (genetics), Influenza B virus (genetics), Influenza, Human (diagnosis), Influenza, Human (virology), RNA, Viral (analysis), Respiratory System (anatomy & histology), Respiratory System (virology), Reverse Transcriptase Polymerase Chain Reaction (methods).
- MESH :
- chemical , analysis : RNA, Viral.
- anatomy & histology : Respiratory System.
- diagnosis : Influenza, Human.
- genetics : Influenza A virus, Influenza B virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Influenza, Human, Respiratory System.
- Autopsy, Humans, Immunohistochemistry.
Abstract
The recent influenza pandemic, caused by a novel H1N1 influenza A virus, as well as the seasonal influenza outbreaks caused by varieties of influenza A and B viruses, are responsible for hundreds of thousands of deaths worldwide. Few studies have evaluated the utility of real-time reverse transcription-PCR to detect influenza virus RNA from formalin-fixed, paraffin-embedded tissues obtained at autopsy. In this work, respiratory autopsy tissues from 442 suspect influenza cases were tested by real-time reverse transcription-PCR for seasonal influenza A and B and 2009 pandemic influenza A (H1N1) viruses and the results were compared to those obtained by immunohistochemistry. In total, 222 cases were positive by real-time reverse transcription-PCR, and of 218 real-time, reverse transcription-PCR-positive cases also tested by immunohistochemistry, only 107 were positive. Although formalin-fixed, paraffin-embedded tissues can be used for diagnosis, frozen tissues offer the best chance to make a postmortem diagnosis of influenza because these tissues possess nucleic acids that are less degraded and, as a consequence, provide longer sequence information than that obtained from fixed tissues. We also determined that testing of all available respiratory tissues is critical for optimal detection of influenza virus in postmortem tissues.
Url:
DOI: 10.1016/j.jmoldx.2010.09.004
PubMed: 21354045
PubMed Central: 3128551
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000726
- to stream Pmc, to step Curation: 000726
- to stream Pmc, to step Checkpoint: 000901
- to stream PubMed, to step Corpus: 001090
- to stream PubMed, to step Curation: 001090
- to stream PubMed, to step Checkpoint: 001015
- to stream Ncbi, to step Merge: 000F71
- to stream Ncbi, to step Curation: 000F71
- to stream Ncbi, to step Checkpoint: 000F71
- to stream Main, to step Merge: 002638
- to stream Main, to step Curation: 002545
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Diagnosis of Influenza from Respiratory Autopsy Tissues</title><author><name sortKey="Denison, Amy M" sort="Denison, Amy M" uniqKey="Denison A" first="Amy M." last="Denison">Amy M. Denison</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Blau, Dianna M" sort="Blau, Dianna M" uniqKey="Blau D" first="Dianna M." last="Blau">Dianna M. Blau</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Jost, Heather A" sort="Jost, Heather A" uniqKey="Jost H" first="Heather A." last="Jost">Heather A. Jost</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Jones, Tara" sort="Jones, Tara" uniqKey="Jones T" first="Tara" last="Jones">Tara Jones</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Rollin, Dominique" sort="Rollin, Dominique" uniqKey="Rollin D" first="Dominique" last="Rollin">Dominique Rollin</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Rongbao" sort="Gao, Rongbao" uniqKey="Gao R" first="Rongbao" last="Gao">Rongbao Gao</name><affiliation wicri:level="3"><nlm:aff id="aff3">Influenza Department, National Institute for Viral Disease Control and Prevention, Center for Disease Control and Prevention, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Influenza Department, National Institute for Viral Disease Control and Prevention, Center for Disease Control and Prevention, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Lindy" sort="Liu, Lindy" uniqKey="Liu L" first="Lindy" last="Liu">Lindy Liu</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Bhatnagar, Julu" sort="Bhatnagar, Julu" uniqKey="Bhatnagar J" first="Julu" last="Bhatnagar">Julu Bhatnagar</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Deleon Carnes, Marlene" sort="Deleon Carnes, Marlene" uniqKey="Deleon Carnes M" first="Marlene" last="Deleon-Carnes">Marlene Deleon-Carnes</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Shieh, Wun Ju" sort="Shieh, Wun Ju" uniqKey="Shieh W" first="Wun-Ju" last="Shieh">Wun-Ju Shieh</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Paddock, Christopher D" sort="Paddock, Christopher D" uniqKey="Paddock C" first="Christopher D." last="Paddock">Christopher D. Paddock</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Drew, Clifton" sort="Drew, Clifton" uniqKey="Drew C" first="Clifton" last="Drew">Clifton Drew</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Adem, Patricia" sort="Adem, Patricia" uniqKey="Adem P" first="Patricia" last="Adem">Patricia Adem</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Emery, Shannon L" sort="Emery, Shannon L" uniqKey="Emery S" first="Shannon L." last="Emery">Shannon L. Emery</name><affiliation wicri:level="1"><nlm:aff id="aff2">Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Shu, Bo" sort="Shu, Bo" uniqKey="Shu B" first="Bo" last="Shu">Bo Shu</name><affiliation wicri:level="1"><nlm:aff id="aff2">Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Kai Hui" sort="Wu, Kai Hui" uniqKey="Wu K" first="Kai-Hui" last="Wu">Kai-Hui Wu</name><affiliation wicri:level="1"><nlm:aff id="aff2">Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Batten, Brigid" sort="Batten, Brigid" uniqKey="Batten B" first="Brigid" last="Batten">Brigid Batten</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Greer, Patricia W" sort="Greer, Patricia W" uniqKey="Greer P" first="Patricia W." last="Greer">Patricia W. Greer</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Smith, Chalanda S" sort="Smith, Chalanda S" uniqKey="Smith C" first="Chalanda S." last="Smith">Chalanda S. Smith</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Bartlett, Jeanine" sort="Bartlett, Jeanine" uniqKey="Bartlett J" first="Jeanine" last="Bartlett">Jeanine Bartlett</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Montague, Jeltley L" sort="Montague, Jeltley L" uniqKey="Montague J" first="Jeltley L." last="Montague">Jeltley L. Montague</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Patel, Mitesh" sort="Patel, Mitesh" uniqKey="Patel M" first="Mitesh" last="Patel">Mitesh Patel</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Xiyan" sort="Xu, Xiyan" uniqKey="Xu X" first="Xiyan" last="Xu">Xiyan Xu</name><affiliation wicri:level="1"><nlm:aff id="aff2">Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Lindstrom, Stephen" sort="Lindstrom, Stephen" uniqKey="Lindstrom S" first="Stephen" last="Lindstrom">Stephen Lindstrom</name><affiliation wicri:level="1"><nlm:aff id="aff2">Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Klimov, Alexander I" sort="Klimov, Alexander I" uniqKey="Klimov A" first="Alexander I." last="Klimov">Alexander I. Klimov</name><affiliation wicri:level="1"><nlm:aff id="aff2">Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Zaki, Sherif R" sort="Zaki, Sherif R" uniqKey="Zaki S" first="Sherif R." last="Zaki">Sherif R. Zaki</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">21354045</idno><idno type="pmc">3128551</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128551</idno><idno type="RBID">PMC:3128551</idno><idno type="doi">10.1016/j.jmoldx.2010.09.004</idno><date when="2011">2011</date><idno type="wicri:Area/Pmc/Corpus">000726</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000726</idno><idno type="wicri:Area/Pmc/Curation">000726</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000726</idno><idno type="wicri:Area/Pmc/Checkpoint">000901</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000901</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:21354045</idno><idno type="wicri:Area/PubMed/Corpus">001090</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001090</idno><idno type="wicri:Area/PubMed/Curation">001090</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001090</idno><idno type="wicri:Area/PubMed/Checkpoint">001015</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001015</idno><idno type="wicri:Area/Ncbi/Merge">000F71</idno><idno type="wicri:Area/Ncbi/Curation">000F71</idno><idno type="wicri:Area/Ncbi/Checkpoint">000F71</idno><idno type="wicri:doubleKey">1525-1578:2011:Denison A:diagnosis:of:influenza</idno><idno type="wicri:Area/Main/Merge">002638</idno><idno type="wicri:Area/Main/Curation">002545</idno><idno type="wicri:Area/Main/Exploration">002545</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Diagnosis of Influenza from Respiratory Autopsy Tissues</title><author><name sortKey="Denison, Amy M" sort="Denison, Amy M" uniqKey="Denison A" first="Amy M." last="Denison">Amy M. Denison</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Blau, Dianna M" sort="Blau, Dianna M" uniqKey="Blau D" first="Dianna M." last="Blau">Dianna M. Blau</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Jost, Heather A" sort="Jost, Heather A" uniqKey="Jost H" first="Heather A." last="Jost">Heather A. Jost</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Jones, Tara" sort="Jones, Tara" uniqKey="Jones T" first="Tara" last="Jones">Tara Jones</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Rollin, Dominique" sort="Rollin, Dominique" uniqKey="Rollin D" first="Dominique" last="Rollin">Dominique Rollin</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Rongbao" sort="Gao, Rongbao" uniqKey="Gao R" first="Rongbao" last="Gao">Rongbao Gao</name><affiliation wicri:level="3"><nlm:aff id="aff3">Influenza Department, National Institute for Viral Disease Control and Prevention, Center for Disease Control and Prevention, Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Influenza Department, National Institute for Viral Disease Control and Prevention, Center for Disease Control and Prevention, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Lindy" sort="Liu, Lindy" uniqKey="Liu L" first="Lindy" last="Liu">Lindy Liu</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Bhatnagar, Julu" sort="Bhatnagar, Julu" uniqKey="Bhatnagar J" first="Julu" last="Bhatnagar">Julu Bhatnagar</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Deleon Carnes, Marlene" sort="Deleon Carnes, Marlene" uniqKey="Deleon Carnes M" first="Marlene" last="Deleon-Carnes">Marlene Deleon-Carnes</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Shieh, Wun Ju" sort="Shieh, Wun Ju" uniqKey="Shieh W" first="Wun-Ju" last="Shieh">Wun-Ju Shieh</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Paddock, Christopher D" sort="Paddock, Christopher D" uniqKey="Paddock C" first="Christopher D." last="Paddock">Christopher D. Paddock</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Drew, Clifton" sort="Drew, Clifton" uniqKey="Drew C" first="Clifton" last="Drew">Clifton Drew</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Adem, Patricia" sort="Adem, Patricia" uniqKey="Adem P" first="Patricia" last="Adem">Patricia Adem</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Emery, Shannon L" sort="Emery, Shannon L" uniqKey="Emery S" first="Shannon L." last="Emery">Shannon L. Emery</name><affiliation wicri:level="1"><nlm:aff id="aff2">Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Shu, Bo" sort="Shu, Bo" uniqKey="Shu B" first="Bo" last="Shu">Bo Shu</name><affiliation wicri:level="1"><nlm:aff id="aff2">Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Kai Hui" sort="Wu, Kai Hui" uniqKey="Wu K" first="Kai-Hui" last="Wu">Kai-Hui Wu</name><affiliation wicri:level="1"><nlm:aff id="aff2">Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Batten, Brigid" sort="Batten, Brigid" uniqKey="Batten B" first="Brigid" last="Batten">Brigid Batten</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Greer, Patricia W" sort="Greer, Patricia W" uniqKey="Greer P" first="Patricia W." last="Greer">Patricia W. Greer</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Smith, Chalanda S" sort="Smith, Chalanda S" uniqKey="Smith C" first="Chalanda S." last="Smith">Chalanda S. Smith</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Bartlett, Jeanine" sort="Bartlett, Jeanine" uniqKey="Bartlett J" first="Jeanine" last="Bartlett">Jeanine Bartlett</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Montague, Jeltley L" sort="Montague, Jeltley L" uniqKey="Montague J" first="Jeltley L." last="Montague">Jeltley L. Montague</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Patel, Mitesh" sort="Patel, Mitesh" uniqKey="Patel M" first="Mitesh" last="Patel">Mitesh Patel</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Xiyan" sort="Xu, Xiyan" uniqKey="Xu X" first="Xiyan" last="Xu">Xiyan Xu</name><affiliation wicri:level="1"><nlm:aff id="aff2">Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Lindstrom, Stephen" sort="Lindstrom, Stephen" uniqKey="Lindstrom S" first="Stephen" last="Lindstrom">Stephen Lindstrom</name><affiliation wicri:level="1"><nlm:aff id="aff2">Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Klimov, Alexander I" sort="Klimov, Alexander I" uniqKey="Klimov A" first="Alexander I." last="Klimov">Alexander I. Klimov</name><affiliation wicri:level="1"><nlm:aff id="aff2">Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Viral Surveillance and Diagnostic Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Zaki, Sherif R" sort="Zaki, Sherif R" uniqKey="Zaki S" first="Sherif R." last="Zaki">Sherif R. Zaki</name><affiliation wicri:level="1"><nlm:aff id="aff1">Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia</nlm:aff><country xml:lang="fr">Géorgie (pays)</country><wicri:regionArea>Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta</wicri:regionArea><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author></analytic><series><title level="j">The Journal of Molecular Diagnostics : JMD</title><idno type="ISSN">1525-1578</idno><idno type="eISSN">1943-7811</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Autopsy</term><term>Humans</term><term>Immunohistochemistry</term><term>Influenza A virus (genetics)</term><term>Influenza B virus (genetics)</term><term>Influenza, Human (diagnosis)</term><term>Influenza, Human (virology)</term><term>RNA, Viral (analysis)</term><term>Respiratory System (anatomy & histology)</term><term>Respiratory System (virology)</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term><term>Appareil respiratoire (anatomie et histologie)</term><term>Appareil respiratoire (virologie)</term><term>Autopsie</term><term>Grippe humaine (diagnostic)</term><term>Grippe humaine (virologie)</term><term>Humains</term><term>Immunohistochimie</term><term>RT-PCR ()</term><term>Virus de la grippe A (génétique)</term><term>Virus influenza B (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="anatomie et histologie" xml:lang="fr"><term>Appareil respiratoire</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Respiratory System</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term><term>Influenza B virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus de la grippe A</term><term>Virus influenza B</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Appareil respiratoire</term><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza, Human</term><term>Respiratory System</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Autopsy</term><term>Humans</term><term>Immunohistochemistry</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Autopsie</term><term>Humains</term><term>Immunohistochimie</term><term>RT-PCR</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The recent influenza pandemic, caused by a novel H1N1 influenza A virus, as well as the seasonal influenza outbreaks caused by varieties of influenza A and B viruses, are responsible for hundreds of thousands of deaths worldwide. Few studies have evaluated the utility of real-time reverse transcription-PCR to detect influenza virus RNA from formalin-fixed, paraffin-embedded tissues obtained at autopsy. In this work, respiratory autopsy tissues from 442 suspect influenza cases were tested by real-time reverse transcription-PCR for seasonal influenza A and B and 2009 pandemic influenza A (H1N1) viruses and the results were compared to those obtained by immunohistochemistry. In total, 222 cases were positive by real-time reverse transcription-PCR, and of 218 real-time, reverse transcription-PCR-positive cases also tested by immunohistochemistry, only 107 were positive. Although formalin-fixed, paraffin-embedded tissues can be used for diagnosis, frozen tissues offer the best chance to make a postmortem diagnosis of influenza because these tissues possess nucleic acids that are less degraded and, as a consequence, provide longer sequence information than that obtained from fixed tissues. We also determined that testing of all available respiratory tissues is critical for optimal detection of influenza virus in postmortem tissues.</p></div></front></TEI><affiliations><list><country><li>Géorgie (pays)</li><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="Géorgie (pays)"><noRegion><name sortKey="Denison, Amy M" sort="Denison, Amy M" uniqKey="Denison A" first="Amy M." last="Denison">Amy M. Denison</name></noRegion><name sortKey="Adem, Patricia" sort="Adem, Patricia" uniqKey="Adem P" first="Patricia" last="Adem">Patricia Adem</name><name sortKey="Bartlett, Jeanine" sort="Bartlett, Jeanine" uniqKey="Bartlett J" first="Jeanine" last="Bartlett">Jeanine Bartlett</name><name sortKey="Batten, Brigid" sort="Batten, Brigid" uniqKey="Batten B" first="Brigid" last="Batten">Brigid Batten</name><name sortKey="Bhatnagar, Julu" sort="Bhatnagar, Julu" uniqKey="Bhatnagar J" first="Julu" last="Bhatnagar">Julu Bhatnagar</name><name sortKey="Blau, Dianna M" sort="Blau, Dianna M" uniqKey="Blau D" first="Dianna M." last="Blau">Dianna M. Blau</name><name sortKey="Deleon Carnes, Marlene" sort="Deleon Carnes, Marlene" uniqKey="Deleon Carnes M" first="Marlene" last="Deleon-Carnes">Marlene Deleon-Carnes</name><name sortKey="Drew, Clifton" sort="Drew, Clifton" uniqKey="Drew C" first="Clifton" last="Drew">Clifton Drew</name><name sortKey="Emery, Shannon L" sort="Emery, Shannon L" uniqKey="Emery S" first="Shannon L." last="Emery">Shannon L. Emery</name><name sortKey="Greer, Patricia W" sort="Greer, Patricia W" uniqKey="Greer P" first="Patricia W." last="Greer">Patricia W. Greer</name><name sortKey="Jones, Tara" sort="Jones, Tara" uniqKey="Jones T" first="Tara" last="Jones">Tara Jones</name><name sortKey="Jost, Heather A" sort="Jost, Heather A" uniqKey="Jost H" first="Heather A." last="Jost">Heather A. Jost</name><name sortKey="Klimov, Alexander I" sort="Klimov, Alexander I" uniqKey="Klimov A" first="Alexander I." last="Klimov">Alexander I. Klimov</name><name sortKey="Lindstrom, Stephen" sort="Lindstrom, Stephen" uniqKey="Lindstrom S" first="Stephen" last="Lindstrom">Stephen Lindstrom</name><name sortKey="Liu, Lindy" sort="Liu, Lindy" uniqKey="Liu L" first="Lindy" last="Liu">Lindy Liu</name><name sortKey="Montague, Jeltley L" sort="Montague, Jeltley L" uniqKey="Montague J" first="Jeltley L." last="Montague">Jeltley L. Montague</name><name sortKey="Paddock, Christopher D" sort="Paddock, Christopher D" uniqKey="Paddock C" first="Christopher D." last="Paddock">Christopher D. Paddock</name><name sortKey="Patel, Mitesh" sort="Patel, Mitesh" uniqKey="Patel M" first="Mitesh" last="Patel">Mitesh Patel</name><name sortKey="Rollin, Dominique" sort="Rollin, Dominique" uniqKey="Rollin D" first="Dominique" last="Rollin">Dominique Rollin</name><name sortKey="Shieh, Wun Ju" sort="Shieh, Wun Ju" uniqKey="Shieh W" first="Wun-Ju" last="Shieh">Wun-Ju Shieh</name><name sortKey="Shu, Bo" sort="Shu, Bo" uniqKey="Shu B" first="Bo" last="Shu">Bo Shu</name><name sortKey="Smith, Chalanda S" sort="Smith, Chalanda S" uniqKey="Smith C" first="Chalanda S." last="Smith">Chalanda S. Smith</name><name sortKey="Wu, Kai Hui" sort="Wu, Kai Hui" uniqKey="Wu K" first="Kai-Hui" last="Wu">Kai-Hui Wu</name><name sortKey="Xu, Xiyan" sort="Xu, Xiyan" uniqKey="Xu X" first="Xiyan" last="Xu">Xiyan Xu</name><name sortKey="Zaki, Sherif R" sort="Zaki, Sherif R" uniqKey="Zaki S" first="Sherif R." last="Zaki">Sherif R. Zaki</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Gao, Rongbao" sort="Gao, Rongbao" uniqKey="Gao R" first="Rongbao" last="Gao">Rongbao Gao</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/PandemieGrippaleV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002545 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002545 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= PandemieGrippaleV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:3128551
|texte= Diagnosis of Influenza from Respiratory Autopsy Tissues
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:21354045" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PandemieGrippaleV1
| This area was generated with Dilib version V0.6.34. |  |