Antibody Recognition of a Highly Conserved Influenza Virus Epitope
Identifieur interne : 004459 ( Main/Exploration ); précédent : 004458; suivant : 004460Antibody Recognition of a Highly Conserved Influenza Virus Epitope
Auteurs : Damian C. Ekiert [États-Unis] ; Gira Bhabha [États-Unis] ; Marc-André Elsliger [États-Unis] ; Robert H. E. Friesen [Pays-Bas] ; Mandy Jongeneelen [Pays-Bas] ; Mark Throsby [Pays-Bas] ; Jaap Goudsmit [Pays-Bas] ; Ian A. Wilson [États-Unis]Source :
- Science : (Washington, D.C.) [ 0036-8075 ] ; 2009.
Descripteurs français
- KwdFr :
- Affinité des anticorps, Anticorps antiviraux (), Anticorps antiviraux (immunologie), Antigènes viraux (), Antigènes viraux (immunologie), Concentration en ions d'hydrogène, Conformation des protéines, Cristallisation, Cristallographie aux rayons X, Fragments Fab d'immunoglobuline (), Fragments Fab d'immunoglobuline (immunologie), Fusion membranaire, Glycoprotéine hémagglutinine du virus influenza (), Glycoprotéine hémagglutinine du virus influenza (immunologie), Glycosylation, Humains, Interactions hydrophobes et hydrophiles, Liaison hydrogène, Modèles moléculaires, Sites de fixation des anticorps, Sous-type H1N1 du virus de la grippe A (immunologie), Sous-type H5N1 du virus de la grippe A (immunologie), Structure secondaire des protéines, Structure tertiaire des protéines, Tests de neutralisation, Vaccins antigrippaux, Épitopes (immunologie).
- MESH :
- Pascal (Inist)
- Affinité des anticorps, Anticorps antiviraux, Antigènes viraux, Concentration en ions d'hydrogène, Conformation des protéines, Cristallisation, Cristallographie aux rayons X, Fragments Fab d'immunoglobuline, Fusion membranaire, Glycoprotéine hémagglutinine du virus influenza, Glycosylation, Humains, Interactions hydrophobes et hydrophiles, Liaison hydrogène, Modèles moléculaires, Sites de fixation des anticorps, Structure secondaire des protéines, Structure tertiaire des protéines, Tests de neutralisation, Vaccins antigrippaux, Virus grippal A, Déterminant antigénique, Reconnaissance, Anticorps, Fragment peptidique Fab, Mécanisme action.
English descriptors
- KwdEn :
- Antibodies, Viral (chemistry), Antibodies, Viral (immunology), Antibody, Antibody Affinity, Antigenic determinant, Antigens, Viral (chemistry), Antigens, Viral (immunology), Binding Sites, Antibody, Crystallization, Crystallography, X-Ray, Epitopes (immunology), Fab-Fragment, Glycosylation, Hemagglutinin Glycoproteins, Influenza Virus (chemistry), Hemagglutinin Glycoproteins, Influenza Virus (immunology), Humans, Hydrogen Bonding, Hydrogen-Ion Concentration, Hydrophobic and Hydrophilic Interactions, Immunoglobulin Fab Fragments (chemistry), Immunoglobulin Fab Fragments (immunology), Influenza A Virus, H1N1 Subtype (immunology), Influenza A Virus, H5N1 Subtype (immunology), Influenza A virus, Influenza Vaccines, Mechanism of action, Membrane Fusion, Models, Molecular, Neutralization Tests, Protein Conformation, Protein Structure, Secondary, Protein Structure, Tertiary, Recognition.
- MESH :
- chemical , chemistry : Antibodies, Viral, Antigens, Viral, Hemagglutinin Glycoproteins, Influenza Virus, Immunoglobulin Fab Fragments.
- chemical , immunology : Antibodies, Viral, Antigens, Viral, Epitopes, Hemagglutinin Glycoproteins, Influenza Virus, Immunoglobulin Fab Fragments.
- immunology : Influenza A Virus, H1N1 Subtype, Influenza A Virus, H5N1 Subtype.
- Antibody Affinity, Binding Sites, Antibody, Crystallization, Crystallography, X-Ray, Glycosylation, Humans, Hydrogen Bonding, Hydrogen-Ion Concentration, Hydrophobic and Hydrophilic Interactions, Influenza Vaccines, Membrane Fusion, Models, Molecular, Neutralization Tests, Protein Conformation, Protein Structure, Secondary, Protein Structure, Tertiary.
Abstract
Influenza virus presents an important and persistent threat to public health worldwide, and current vaccines provide immunity to viral isolates similar to the vaccine strain. High-affinity antibodies against a conserved epitope could provide immunity to the diverse influenza subtypes and protection against future pandemic viruses. Cocrystal structures were determined at 2.2 and 2.7 angstrom resolutions for broadly neutralizing human antibody CR6261 Fab in complexes with the major surface antigen (hemagglutinin, HA) from viruses responsible for the 1918 H1N1 influenza pandemic and a recent lethal case of H5N1 avian influenza. In contrast to other structurally characterized influenza antibodies, CR6261 recognizes a highly conserved helical region in the membrane-proximal stem of HA1 and HA2. The antibody neutralizes the virus by blocking conformational rearrangements associated with membrane fusion. The CR6261 epitope identified here should accelerate the design and implementation of improved vaccines that can elicit CR6261-like antibodies, as well as antibody-based therapies for the treatment of influenza.
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Affiliations:
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Le document en format XML
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (chemistry)</term>
<term>Antibodies, Viral (immunology)</term>
<term>Antibody</term>
<term>Antibody Affinity</term>
<term>Antigenic determinant</term>
<term>Antigens, Viral (chemistry)</term>
<term>Antigens, Viral (immunology)</term>
<term>Binding Sites, Antibody</term>
<term>Crystallization</term>
<term>Crystallography, X-Ray</term>
<term>Epitopes (immunology)</term>
<term>Fab-Fragment</term>
<term>Glycosylation</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (chemistry)</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (immunology)</term>
<term>Humans</term>
<term>Hydrogen Bonding</term>
<term>Hydrogen-Ion Concentration</term>
<term>Hydrophobic and Hydrophilic Interactions</term>
<term>Immunoglobulin Fab Fragments (chemistry)</term>
<term>Immunoglobulin Fab Fragments (immunology)</term>
<term>Influenza A Virus, H1N1 Subtype (immunology)</term>
<term>Influenza A Virus, H5N1 Subtype (immunology)</term>
<term>Influenza A virus</term>
<term>Influenza Vaccines</term>
<term>Mechanism of action</term>
<term>Membrane Fusion</term>
<term>Models, Molecular</term>
<term>Neutralization Tests</term>
<term>Protein Conformation</term>
<term>Protein Structure, Secondary</term>
<term>Protein Structure, Tertiary</term>
<term>Recognition</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Affinité des anticorps</term>
<term>Anticorps antiviraux ()</term>
<term>Anticorps antiviraux (immunologie)</term>
<term>Antigènes viraux ()</term>
<term>Antigènes viraux (immunologie)</term>
<term>Concentration en ions d'hydrogène</term>
<term>Conformation des protéines</term>
<term>Cristallisation</term>
<term>Cristallographie aux rayons X</term>
<term>Fragments Fab d'immunoglobuline ()</term>
<term>Fragments Fab d'immunoglobuline (immunologie)</term>
<term>Fusion membranaire</term>
<term>Glycoprotéine hémagglutinine du virus influenza ()</term>
<term>Glycoprotéine hémagglutinine du virus influenza (immunologie)</term>
<term>Glycosylation</term>
<term>Humains</term>
<term>Interactions hydrophobes et hydrophiles</term>
<term>Liaison hydrogène</term>
<term>Modèles moléculaires</term>
<term>Sites de fixation des anticorps</term>
<term>Sous-type H1N1 du virus de la grippe A (immunologie)</term>
<term>Sous-type H5N1 du virus de la grippe A (immunologie)</term>
<term>Structure secondaire des protéines</term>
<term>Structure tertiaire des protéines</term>
<term>Tests de neutralisation</term>
<term>Vaccins antigrippaux</term>
<term>Épitopes (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antibodies, Viral</term>
<term>Antigens, Viral</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus</term>
<term>Immunoglobulin Fab Fragments</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term>
<term>Antigens, Viral</term>
<term>Epitopes</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus</term>
<term>Immunoglobulin Fab Fragments</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Antigènes viraux</term>
<term>Fragments Fab d'immunoglobuline</term>
<term>Glycoprotéine hémagglutinine du virus influenza</term>
<term>Sous-type H1N1 du virus de la grippe A</term>
<term>Sous-type H5N1 du virus de la grippe A</term>
<term>Épitopes</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term>
<term>Influenza A Virus, H5N1 Subtype</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Antibody Affinity</term>
<term>Binding Sites, Antibody</term>
<term>Crystallization</term>
<term>Crystallography, X-Ray</term>
<term>Glycosylation</term>
<term>Humans</term>
<term>Hydrogen Bonding</term>
<term>Hydrogen-Ion Concentration</term>
<term>Hydrophobic and Hydrophilic Interactions</term>
<term>Influenza Vaccines</term>
<term>Membrane Fusion</term>
<term>Models, Molecular</term>
<term>Neutralization Tests</term>
<term>Protein Conformation</term>
<term>Protein Structure, Secondary</term>
<term>Protein Structure, Tertiary</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Affinité des anticorps</term>
<term>Anticorps antiviraux</term>
<term>Antigènes viraux</term>
<term>Concentration en ions d'hydrogène</term>
<term>Conformation des protéines</term>
<term>Cristallisation</term>
<term>Cristallographie aux rayons X</term>
<term>Fragments Fab d'immunoglobuline</term>
<term>Fusion membranaire</term>
<term>Glycoprotéine hémagglutinine du virus influenza</term>
<term>Glycosylation</term>
<term>Humains</term>
<term>Interactions hydrophobes et hydrophiles</term>
<term>Liaison hydrogène</term>
<term>Modèles moléculaires</term>
<term>Sites de fixation des anticorps</term>
<term>Structure secondaire des protéines</term>
<term>Structure tertiaire des protéines</term>
<term>Tests de neutralisation</term>
<term>Vaccins antigrippaux</term>
<term>Virus grippal A</term>
<term>Déterminant antigénique</term>
<term>Reconnaissance</term>
<term>Anticorps</term>
<term>Fragment peptidique Fab</term>
<term>Mécanisme action</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Influenza virus presents an important and persistent threat to public health worldwide, and current vaccines provide immunity to viral isolates similar to the vaccine strain. High-affinity antibodies against a conserved epitope could provide immunity to the diverse influenza subtypes and protection against future pandemic viruses. Cocrystal structures were determined at 2.2 and 2.7 angstrom resolutions for broadly neutralizing human antibody CR6261 Fab in complexes with the major surface antigen (hemagglutinin, HA) from viruses responsible for the 1918 H1N1 influenza pandemic and a recent lethal case of H5N1 avian influenza. In contrast to other structurally characterized influenza antibodies, CR6261 recognizes a highly conserved helical region in the membrane-proximal stem of HA1 and HA2. The antibody neutralizes the virus by blocking conformational rearrangements associated with membrane fusion. The CR6261 epitope identified here should accelerate the design and implementation of improved vaccines that can elicit CR6261-like antibodies, as well as antibody-based therapies for the treatment of influenza.</div>
</front>
</TEI>
<affiliations><list><country><li>Pays-Bas</li>
<li>États-Unis</li>
</country>
<region><li>Californie</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Californie"><name sortKey="Ekiert, Damian C" sort="Ekiert, Damian C" uniqKey="Ekiert D" first="Damian C." last="Ekiert">Damian C. Ekiert</name>
</region>
<name sortKey="Bhabha, Gira" sort="Bhabha, Gira" uniqKey="Bhabha G" first="Gira" last="Bhabha">Gira Bhabha</name>
<name sortKey="Elsliger, Marc Andre" sort="Elsliger, Marc Andre" uniqKey="Elsliger M" first="Marc-André" last="Elsliger">Marc-André Elsliger</name>
<name sortKey="Wilson, Ian A" sort="Wilson, Ian A" uniqKey="Wilson I" first="Ian A." last="Wilson">Ian A. Wilson</name>
<name sortKey="Wilson, Ian A" sort="Wilson, Ian A" uniqKey="Wilson I" first="Ian A." last="Wilson">Ian A. Wilson</name>
</country>
<country name="Pays-Bas"><noRegion><name sortKey="Friesen, Robert H E" sort="Friesen, Robert H E" uniqKey="Friesen R" first="Robert H. E." last="Friesen">Robert H. E. Friesen</name>
</noRegion>
<name sortKey="Goudsmit, Jaap" sort="Goudsmit, Jaap" uniqKey="Goudsmit J" first="Jaap" last="Goudsmit">Jaap Goudsmit</name>
<name sortKey="Jongeneelen, Mandy" sort="Jongeneelen, Mandy" uniqKey="Jongeneelen M" first="Mandy" last="Jongeneelen">Mandy Jongeneelen</name>
<name sortKey="Throsby, Mark" sort="Throsby, Mark" uniqKey="Throsby M" first="Mark" last="Throsby">Mark Throsby</name>
</country>
</tree>
</affiliations>
</record>
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