Productive Infection of Human Skeletal Muscle Cells by Pandemic and Seasonal Influenza A(H1N1) Viruses
Identifieur interne : 001184 ( Main/Exploration ); précédent : 001183; suivant : 001185Productive Infection of Human Skeletal Muscle Cells by Pandemic and Seasonal Influenza A(H1N1) Viruses
Auteurs : Marion Desdouits [France] ; Sandie Munier [France] ; Marie-Christine Prevost [France] ; Patricia Jeannin [France] ; Gillian Butler-Browne [France] ; Simona Ozden [France] ; Antoine Gessain [France] ; Sylvie Van Der Werf [France] ; Nadia Naffakh [France] ; Pierre-Emmanuel Ceccaldi [France]Source :
- PLoS ONE [ 1932-6203 ] ; 2013.
Abstract
Besides the classical respiratory and systemic symptoms, unusual complications of influenza A infection in humans involve the skeletal muscles. Numerous cases of acute myopathy and/or rhabdomyolysis have been reported, particularly following the outbreak of pandemic influenza A(H1N1) in 2009. The pathogenesis of these influenza-associated myopathies (IAM) remains unkown, although the direct infection of muscle cells is suspected. Here, we studied the susceptibility of cultured human primary muscle cells to a 2009 pandemic and a 2008 seasonal influenza A(H1N1) isolate. Using cells from different donors, we found that differentiated muscle cells (i. e. myotubes) were highly susceptible to infection by both influenza A(H1N1) isolates, whereas undifferentiated cells (i. e. myoblasts) were partially resistant. The receptors for influenza viruses, α2-6 and α2-3 linked sialic acids, were detected on the surface of myotubes and myoblasts. Time line of viral nucleoprotein (NP) expression and nuclear export showed that the first steps of the viral replication cycle could take place in muscle cells. Infected myotubes and myoblasts exhibited budding virions and nuclear inclusions as observed by transmission electron microscopy and correlative light and electron microscopy. Myotubes, but not myoblasts, yielded infectious virus progeny that could further infect naive muscle cells after proteolytic treatment. Infection led to a cytopathic effect with the lysis of muscle cells, as characterized by the release of lactate dehydrogenase. The secretion of proinflammatory cytokines by muscle cells was not affected following infection. Our results are compatible with the hypothesis of a direct muscle infection causing rhabdomyolysis in IAM patients.
Url:
DOI: 10.1371/journal.pone.0079628
Affiliations:
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Le document en format XML
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<author><name sortKey="Gessain, Antoine" sort="Gessain, Antoine" uniqKey="Gessain A" first="Antoine" last="Gessain">Antoine Gessain</name>
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<author><name sortKey="Van Der Werf, Sylvie" sort="Van Der Werf, Sylvie" uniqKey="Van Der Werf S" first="Sylvie" last="Van Der Werf">Sylvie Van Der Werf</name>
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<idno type="RNSR">200118187U</idno>
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<country>France</country>
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</author>
<author><name sortKey="Naffakh, Nadia" sort="Naffakh, Nadia" uniqKey="Naffakh N" first="Nadia" last="Naffakh">Nadia Naffakh</name>
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<idno type="RNSR">200818198A</idno>
<orgName>Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses</orgName>
<orgName type="acronym">GMV-ARN (UMR_3569 / U-Pasteur_2)</orgName>
<date type="end">2019-12-31</date>
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<country>France</country>
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</author>
<author><name sortKey="Ceccaldi, Pierre Emmanuel" sort="Ceccaldi, Pierre Emmanuel" uniqKey="Ceccaldi P" first="Pierre-Emmanuel" last="Ceccaldi">Pierre-Emmanuel Ceccaldi</name>
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<idno type="RNSR">200118187U</idno>
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<orgName type="acronym">EPVO (UMR_3569 / U-Pasteur_3)</orgName>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Département de Virologie, 25-28 rue du Docteur Roux F-75724 Paris Cedex 15</addrLine>
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</address>
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</address>
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<orgName>Université Paris Diderot - Paris 7</orgName>
<orgName type="acronym">UPD7</orgName>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine>
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<orgName type="acronym">CNRS</orgName>
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</address>
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<country>France</country>
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</author>
</analytic>
<idno type="DOI">10.1371/journal.pone.0079628</idno>
<series><title level="j">PLoS ONE</title>
<idno type="ISSN">1932-6203</idno>
<imprint><date type="datePub">2013</date>
</imprint>
</series>
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<front><div type="abstract" xml:lang="en"> <p>Besides the classical respiratory and systemic symptoms, unusual complications of influenza A infection in humans involve the skeletal muscles. Numerous cases of acute myopathy and/or rhabdomyolysis have been reported, particularly following the outbreak of pandemic influenza A(H1N1) in 2009. The pathogenesis of these influenza-associated myopathies (IAM) remains unkown, although the direct infection of muscle cells is suspected. Here, we studied the susceptibility of cultured human primary muscle cells to a 2009 pandemic and a 2008 seasonal influenza A(H1N1) isolate. Using cells from different donors, we found that differentiated muscle cells (i. e. myotubes) were highly susceptible to infection by both influenza A(H1N1) isolates, whereas undifferentiated cells (i. e. myoblasts) were partially resistant. The receptors for influenza viruses, α2-6 and α2-3 linked sialic acids, were detected on the surface of myotubes and myoblasts. Time line of viral nucleoprotein (NP) expression and nuclear export showed that the first steps of the viral replication cycle could take place in muscle cells. Infected myotubes and myoblasts exhibited budding virions and nuclear inclusions as observed by transmission electron microscopy and correlative light and electron microscopy. Myotubes, but not myoblasts, yielded infectious virus progeny that could further infect naive muscle cells after proteolytic treatment. Infection led to a cytopathic effect with the lysis of muscle cells, as characterized by the release of lactate dehydrogenase. The secretion of proinflammatory cytokines by muscle cells was not affected following infection. Our results are compatible with the hypothesis of a direct muscle infection causing rhabdomyolysis in IAM patients.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
</country>
</list>
<tree><country name="France"><noRegion><name sortKey="Desdouits, Marion" sort="Desdouits, Marion" uniqKey="Desdouits M" first="Marion" last="Desdouits">Marion Desdouits</name>
</noRegion>
<name sortKey="Butler Browne, Gillian" sort="Butler Browne, Gillian" uniqKey="Butler Browne G" first="Gillian" last="Butler-Browne">Gillian Butler-Browne</name>
<name sortKey="Ceccaldi, Pierre Emmanuel" sort="Ceccaldi, Pierre Emmanuel" uniqKey="Ceccaldi P" first="Pierre-Emmanuel" last="Ceccaldi">Pierre-Emmanuel Ceccaldi</name>
<name sortKey="Gessain, Antoine" sort="Gessain, Antoine" uniqKey="Gessain A" first="Antoine" last="Gessain">Antoine Gessain</name>
<name sortKey="Jeannin, Patricia" sort="Jeannin, Patricia" uniqKey="Jeannin P" first="Patricia" last="Jeannin">Patricia Jeannin</name>
<name sortKey="Munier, Sandie" sort="Munier, Sandie" uniqKey="Munier S" first="Sandie" last="Munier">Sandie Munier</name>
<name sortKey="Naffakh, Nadia" sort="Naffakh, Nadia" uniqKey="Naffakh N" first="Nadia" last="Naffakh">Nadia Naffakh</name>
<name sortKey="Ozden, Simona" sort="Ozden, Simona" uniqKey="Ozden S" first="Simona" last="Ozden">Simona Ozden</name>
<name sortKey="Prevost, Marie Christine" sort="Prevost, Marie Christine" uniqKey="Prevost M" first="Marie-Christine" last="Prevost">Marie-Christine Prevost</name>
<name sortKey="Van Der Werf, Sylvie" sort="Van Der Werf, Sylvie" uniqKey="Van Der Werf S" first="Sylvie" last="Van Der Werf">Sylvie Van Der Werf</name>
</country>
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</affiliations>
</record>
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