1918 Influenza Virus Hemagglutinin (HA) and the Viral RNA Polymerase Complex Enhance Viral Pathogenicity, but Only HA Induces Aberrant Host Responses in Mice
Identifieur interne : 001168 ( Main/Exploration ); précédent : 001167; suivant : 0011691918 Influenza Virus Hemagglutinin (HA) and the Viral RNA Polymerase Complex Enhance Viral Pathogenicity, but Only HA Induces Aberrant Host Responses in Mice
Auteurs : Tokiko Watanabe ; Jennifer Tisoncik-Go ; Nicolas Tchitchek ; Shinji Watanabe ; Arndt G. Benecke [France] ; Michael G. Katze ; Yoshihiro KawaokaSource :
- Journal of Virology [ 0022-538X ] ; 2013-05.
Abstract
The 1918 pandemic influenza virus was the most devastating infectious agent in human history, causing fatal pneumonia and an estimated 20 to 50 million deaths worldwide. Previous studies indicated a prominent role of the hemagglutinin (HA) gene in efficient replication and high virulence of the 1918 virus in mice. It is, however, still unclear whether the high replication ability or the 1918 influenza virus HA gene is required for 1918 virus to exhibit high virulence in mice. Here, we examined the biological properties of reassortant viruses between the 1918 virus and a contemporary human H1N1 virus (A/Kawasaki/173/2001 [K173]) in a mouse model. In addition to the 1918 influenza virus HA, we demonstrated the role of the viral RNA replication complex in efficient replication of viruses in mouse lungs, whereas only the HA gene is responsible for lethality in mice. Global gene expression profiling of infected mouse lungs revealed that the 1918 influenza virus HA was sufficient to induce transcriptional changes similar to those induced by the 1918 virus, despite difference in lymphocyte gene expression. Increased expression of genes associated with the acute-phase response and the protein ubiquitination pathway were enriched during infections with the 1918 and 1918HA/K173 viruses, whereas reassortant viruses bearing the 1918 viral RNA polymerase complex induced transcriptional changes similar to those seen with the K173 virus. Taken together, these data suggest that HA and the viral RNA polymerase complex are critical determinants of Spanish influenza pathogenesis, but only HA, and not the viral RNA polymerase complex and NP, is responsible for extreme host responses observed in mice infected with the 1918 influenza virus.
Url:
DOI: 10.1128/JVI.02753-12
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Hal, to step Corpus: 000009
- to stream Hal, to step Curation: 000009
- to stream Hal, to step Checkpoint: 000475
- to stream Main, to step Merge: 001180
- to stream Main, to step Curation: 001168
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">1918 Influenza Virus Hemagglutinin (HA) and the Viral RNA Polymerase Complex Enhance Viral Pathogenicity, but Only HA Induces Aberrant Host Responses in Mice</title>
<author><name sortKey="Watanabe, Tokiko" sort="Watanabe, Tokiko" uniqKey="Watanabe T" first="Tokiko" last="Watanabe">Tokiko Watanabe</name>
</author>
<author><name sortKey="Tisoncik Go, Jennifer" sort="Tisoncik Go, Jennifer" uniqKey="Tisoncik Go J" first="Jennifer" last="Tisoncik-Go">Jennifer Tisoncik-Go</name>
</author>
<author><name sortKey="Tchitchek, Nicolas" sort="Tchitchek, Nicolas" uniqKey="Tchitchek N" first="Nicolas" last="Tchitchek">Nicolas Tchitchek</name>
</author>
<author><name sortKey="Watanabe, Shinji" sort="Watanabe, Shinji" uniqKey="Watanabe S" first="Shinji" last="Watanabe">Shinji Watanabe</name>
</author>
<author><name sortKey="Benecke, Arndt G" sort="Benecke, Arndt G" uniqKey="Benecke A" first="Arndt G." last="Benecke">Arndt G. Benecke</name>
<affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-498235" status="OLD"> <orgName>Expression des Gènes et comportement adaptatifs = Molecular Genetics, Neurophysiology and Behavior</orgName>
<orgName type="acronym">NPS-15</orgName>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.ibps.upmc.fr/fr/Recherche/umr-8246/expression-des-genes-et-comportement-adaptatifs</ref>
</desc>
<listRelation> <relation active="#struct-246944" type="direct"></relation>
<relation name="UM119" active="#struct-93591" type="indirect"></relation>
<relation active="#struct-262166" type="indirect"></relation>
<relation active="#struct-93591" type="indirect"></relation>
<relation active="#struct-303623" type="indirect"></relation>
<relation name="FR 3631" active="#struct-441569" type="indirect"></relation>
<relation name="U1130" active="#struct-303623" type="indirect"></relation>
<relation name="UMR8246" active="#struct-441569" type="indirect"></relation>
</listRelation>
<tutelles><tutelle active="#struct-246944" type="direct"><org type="laboratory" xml:id="struct-246944" status="OLD"> <idno type="IdRef">227096843</idno>
<idno type="RNSR">201420752A</idno>
<orgName>Neuroscience Paris Seine</orgName>
<orgName type="acronym">NPS</orgName>
<desc> <address> <addrLine>Campus Jussieu - 9 quai Saint-Bernard - Bâtiment B/4ème étage - 75252 Paris cedex 05</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.ibps.upmc.fr/fr/Recherche/umr-8246</ref>
</desc>
<listRelation> <relation name="UM119" active="#struct-93591" type="direct"></relation>
<relation active="#struct-262166" type="direct"></relation>
<relation active="#struct-93591" type="direct"></relation>
<relation active="#struct-303623" type="direct"></relation>
<relation name="FR 3631" active="#struct-441569" type="direct"></relation>
<relation name="U1130" active="#struct-303623" type="direct"></relation>
<relation name="UMR8246" active="#struct-441569" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle name="UM119" active="#struct-93591" type="indirect"><org type="institution" xml:id="struct-93591" status="OLD"> <orgName>Université Pierre et Marie Curie - Paris 6</orgName>
<orgName type="acronym">UPMC</orgName>
<date type="end">2017-12-31</date>
<desc> <address> <addrLine>4 place Jussieu - 75005 Paris</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.upmc.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-262166" type="indirect"><org type="regrouplaboratory" xml:id="struct-262166" status="OLD"> <idno type="RNSR">201420879N</idno>
<orgName>Institut de Biologie Paris Seine</orgName>
<orgName type="acronym">IBPS</orgName>
<date type="start">2014-01-01</date>
<desc> <address> <addrLine>Quai Saint-Bernard75252 PARIS CEDEX 05</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.ibps.upmc.fr/</ref>
</desc>
<listRelation> <relation active="#struct-93591" type="direct"></relation>
<relation active="#struct-303623" type="direct"></relation>
<relation name="FR 3631" active="#struct-441569" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle active="#struct-93591" type="indirect"><org type="institution" xml:id="struct-93591" status="OLD"> <orgName>Université Pierre et Marie Curie - Paris 6</orgName>
<orgName type="acronym">UPMC</orgName>
<date type="end">2017-12-31</date>
<desc> <address> <addrLine>4 place Jussieu - 75005 Paris</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.upmc.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="FR 3631" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="U1130" active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR8246" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Katze, Michael G" sort="Katze, Michael G" uniqKey="Katze M" first="Michael G." last="Katze">Michael G. Katze</name>
</author>
<author><name sortKey="Kawaoka, Yoshihiro" sort="Kawaoka, Yoshihiro" uniqKey="Kawaoka Y" first="Yoshihiro" last="Kawaoka">Yoshihiro Kawaoka</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">HAL</idno>
<idno type="RBID">Hal:hal-01543311</idno>
<idno type="halId">hal-01543311</idno>
<idno type="halUri">https://hal.archives-ouvertes.fr/hal-01543311</idno>
<idno type="url">https://hal.archives-ouvertes.fr/hal-01543311</idno>
<idno type="doi">10.1128/JVI.02753-12</idno>
<date when="2013-05">2013-05</date>
<idno type="wicri:Area/Hal/Corpus">000009</idno>
<idno type="wicri:Area/Hal/Curation">000009</idno>
<idno type="wicri:Area/Hal/Checkpoint">000475</idno>
<idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000475</idno>
<idno type="wicri:doubleKey">0022-538X:2013:Watanabe T:influenza:virus:hemagglutinin</idno>
<idno type="wicri:Area/Main/Merge">001180</idno>
<idno type="wicri:Area/Main/Curation">001168</idno>
<idno type="wicri:Area/Main/Exploration">001168</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">1918 Influenza Virus Hemagglutinin (HA) and the Viral RNA Polymerase Complex Enhance Viral Pathogenicity, but Only HA Induces Aberrant Host Responses in Mice</title>
<author><name sortKey="Watanabe, Tokiko" sort="Watanabe, Tokiko" uniqKey="Watanabe T" first="Tokiko" last="Watanabe">Tokiko Watanabe</name>
</author>
<author><name sortKey="Tisoncik Go, Jennifer" sort="Tisoncik Go, Jennifer" uniqKey="Tisoncik Go J" first="Jennifer" last="Tisoncik-Go">Jennifer Tisoncik-Go</name>
</author>
<author><name sortKey="Tchitchek, Nicolas" sort="Tchitchek, Nicolas" uniqKey="Tchitchek N" first="Nicolas" last="Tchitchek">Nicolas Tchitchek</name>
</author>
<author><name sortKey="Watanabe, Shinji" sort="Watanabe, Shinji" uniqKey="Watanabe S" first="Shinji" last="Watanabe">Shinji Watanabe</name>
</author>
<author><name sortKey="Benecke, Arndt G" sort="Benecke, Arndt G" uniqKey="Benecke A" first="Arndt G." last="Benecke">Arndt G. Benecke</name>
<affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-498235" status="OLD"> <orgName>Expression des Gènes et comportement adaptatifs = Molecular Genetics, Neurophysiology and Behavior</orgName>
<orgName type="acronym">NPS-15</orgName>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.ibps.upmc.fr/fr/Recherche/umr-8246/expression-des-genes-et-comportement-adaptatifs</ref>
</desc>
<listRelation> <relation active="#struct-246944" type="direct"></relation>
<relation name="UM119" active="#struct-93591" type="indirect"></relation>
<relation active="#struct-262166" type="indirect"></relation>
<relation active="#struct-93591" type="indirect"></relation>
<relation active="#struct-303623" type="indirect"></relation>
<relation name="FR 3631" active="#struct-441569" type="indirect"></relation>
<relation name="U1130" active="#struct-303623" type="indirect"></relation>
<relation name="UMR8246" active="#struct-441569" type="indirect"></relation>
</listRelation>
<tutelles><tutelle active="#struct-246944" type="direct"><org type="laboratory" xml:id="struct-246944" status="OLD"> <idno type="IdRef">227096843</idno>
<idno type="RNSR">201420752A</idno>
<orgName>Neuroscience Paris Seine</orgName>
<orgName type="acronym">NPS</orgName>
<desc> <address> <addrLine>Campus Jussieu - 9 quai Saint-Bernard - Bâtiment B/4ème étage - 75252 Paris cedex 05</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.ibps.upmc.fr/fr/Recherche/umr-8246</ref>
</desc>
<listRelation> <relation name="UM119" active="#struct-93591" type="direct"></relation>
<relation active="#struct-262166" type="direct"></relation>
<relation active="#struct-93591" type="direct"></relation>
<relation active="#struct-303623" type="direct"></relation>
<relation name="FR 3631" active="#struct-441569" type="direct"></relation>
<relation name="U1130" active="#struct-303623" type="direct"></relation>
<relation name="UMR8246" active="#struct-441569" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle name="UM119" active="#struct-93591" type="indirect"><org type="institution" xml:id="struct-93591" status="OLD"> <orgName>Université Pierre et Marie Curie - Paris 6</orgName>
<orgName type="acronym">UPMC</orgName>
<date type="end">2017-12-31</date>
<desc> <address> <addrLine>4 place Jussieu - 75005 Paris</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.upmc.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-262166" type="indirect"><org type="regrouplaboratory" xml:id="struct-262166" status="OLD"> <idno type="RNSR">201420879N</idno>
<orgName>Institut de Biologie Paris Seine</orgName>
<orgName type="acronym">IBPS</orgName>
<date type="start">2014-01-01</date>
<desc> <address> <addrLine>Quai Saint-Bernard75252 PARIS CEDEX 05</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.ibps.upmc.fr/</ref>
</desc>
<listRelation> <relation active="#struct-93591" type="direct"></relation>
<relation active="#struct-303623" type="direct"></relation>
<relation name="FR 3631" active="#struct-441569" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle active="#struct-93591" type="indirect"><org type="institution" xml:id="struct-93591" status="OLD"> <orgName>Université Pierre et Marie Curie - Paris 6</orgName>
<orgName type="acronym">UPMC</orgName>
<date type="end">2017-12-31</date>
<desc> <address> <addrLine>4 place Jussieu - 75005 Paris</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.upmc.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="FR 3631" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="U1130" active="#struct-303623" type="indirect"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR8246" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Katze, Michael G" sort="Katze, Michael G" uniqKey="Katze M" first="Michael G." last="Katze">Michael G. Katze</name>
</author>
<author><name sortKey="Kawaoka, Yoshihiro" sort="Kawaoka, Yoshihiro" uniqKey="Kawaoka Y" first="Yoshihiro" last="Kawaoka">Yoshihiro Kawaoka</name>
</author>
</analytic>
<idno type="DOI">10.1128/JVI.02753-12</idno>
<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
<imprint><date type="datePub">2013-05</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"> <p>The 1918 pandemic influenza virus was the most devastating infectious agent in human history, causing fatal pneumonia and an estimated 20 to 50 million deaths worldwide. Previous studies indicated a prominent role of the hemagglutinin (HA) gene in efficient replication and high virulence of the 1918 virus in mice. It is, however, still unclear whether the high replication ability or the 1918 influenza virus HA gene is required for 1918 virus to exhibit high virulence in mice. Here, we examined the biological properties of reassortant viruses between the 1918 virus and a contemporary human H1N1 virus (A/Kawasaki/173/2001 [K173]) in a mouse model. In addition to the 1918 influenza virus HA, we demonstrated the role of the viral RNA replication complex in efficient replication of viruses in mouse lungs, whereas only the HA gene is responsible for lethality in mice. Global gene expression profiling of infected mouse lungs revealed that the 1918 influenza virus HA was sufficient to induce transcriptional changes similar to those induced by the 1918 virus, despite difference in lymphocyte gene expression. Increased expression of genes associated with the acute-phase response and the protein ubiquitination pathway were enriched during infections with the 1918 and 1918HA/K173 viruses, whereas reassortant viruses bearing the 1918 viral RNA polymerase complex induced transcriptional changes similar to those seen with the K173 virus. Taken together, these data suggest that HA and the viral RNA polymerase complex are critical determinants of Spanish influenza pathogenesis, but only HA, and not the viral RNA polymerase complex and NP, is responsible for extreme host responses observed in mice infected with the 1918 influenza virus.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
</country>
</list>
<tree><noCountry><name sortKey="Katze, Michael G" sort="Katze, Michael G" uniqKey="Katze M" first="Michael G." last="Katze">Michael G. Katze</name>
<name sortKey="Kawaoka, Yoshihiro" sort="Kawaoka, Yoshihiro" uniqKey="Kawaoka Y" first="Yoshihiro" last="Kawaoka">Yoshihiro Kawaoka</name>
<name sortKey="Tchitchek, Nicolas" sort="Tchitchek, Nicolas" uniqKey="Tchitchek N" first="Nicolas" last="Tchitchek">Nicolas Tchitchek</name>
<name sortKey="Tisoncik Go, Jennifer" sort="Tisoncik Go, Jennifer" uniqKey="Tisoncik Go J" first="Jennifer" last="Tisoncik-Go">Jennifer Tisoncik-Go</name>
<name sortKey="Watanabe, Shinji" sort="Watanabe, Shinji" uniqKey="Watanabe S" first="Shinji" last="Watanabe">Shinji Watanabe</name>
<name sortKey="Watanabe, Tokiko" sort="Watanabe, Tokiko" uniqKey="Watanabe T" first="Tokiko" last="Watanabe">Tokiko Watanabe</name>
</noCountry>
<country name="France"><noRegion><name sortKey="Benecke, Arndt G" sort="Benecke, Arndt G" uniqKey="Benecke A" first="Arndt G." last="Benecke">Arndt G. Benecke</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/PandemieGrippaleV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001168 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001168 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= PandemieGrippaleV1 |flux= Main |étape= Exploration |type= RBID |clé= Hal:hal-01543311 |texte= 1918 Influenza Virus Hemagglutinin (HA) and the Viral RNA Polymerase Complex Enhance Viral Pathogenicity, but Only HA Induces Aberrant Host Responses in Mice }}
![]() | This area was generated with Dilib version V0.6.34. | ![]() |