Detection of respiratory viruses using a multiplex real-time PCR assay in Germany, 2009/10
Identifieur interne : 001374 ( Main/Exploration ); précédent : 001373; suivant : 001375Detection of respiratory viruses using a multiplex real-time PCR assay in Germany, 2009/10
Auteurs : Sibylle Bierbaum [Allemagne] ; Johannes Forster [Allemagne] ; Reinhard Berner [Allemagne] ; Gerta Rücker [Allemagne] ; Gernot Rohde [Pays-Bas] ; Dieter Neumann-Haefelin [Allemagne] ; Marcus Panning [Allemagne]Source :
- Archives of Virology [ 0304-8608 ] ; 2013.
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Adulte d'âge moyen, Allemagne (épidémiologie), Enfant, Enfant d'âge préscolaire, Femelle, Humains, Infections de l'appareil respiratoire (diagnostic), Infections de l'appareil respiratoire (virologie), Infections de l'appareil respiratoire (épidémiologie), Jeune adulte, Mâle, Nourrisson, Prévalence, Réaction de polymérisation en chaine en temps réel (), Réaction de polymérisation en chaine multiplex (), Sensibilité et spécificité, Techniques de diagnostic moléculaire (), Virologie (), Virus (), Virus (génétique), Virus (isolement et purification), Études prospectives.
- MESH :
- diagnostic : Infections de l'appareil respiratoire.
- génétique : Virus.
- isolement et purification : Virus.
- virologie : Infections de l'appareil respiratoire.
- épidémiologie : Allemagne, Infections de l'appareil respiratoire.
- Adolescent, Adulte, Adulte d'âge moyen, Enfant, Enfant d'âge préscolaire, Femelle, Humains, Jeune adulte, Mâle, Nourrisson, Prévalence, Réaction de polymérisation en chaine en temps réel, Réaction de polymérisation en chaine multiplex, Sensibilité et spécificité, Techniques de diagnostic moléculaire, Virologie, Virus, Études prospectives.
- Wicri :
- geographic : Allemagne.
English descriptors
- KwdEn :
- Adolescent, Adult, Child, Child, Preschool, Female, Germany (epidemiology), Humans, Infant, Male, Middle Aged, Molecular Diagnostic Techniques (methods), Multiplex Polymerase Chain Reaction (methods), Prevalence, Prospective Studies, Real-Time Polymerase Chain Reaction (methods), Respiratory Tract Infections (diagnosis), Respiratory Tract Infections (epidemiology), Respiratory Tract Infections (virology), Sensitivity and Specificity, Virology (methods), Viruses (classification), Viruses (genetics), Viruses (isolation & purification), Young Adult.
- MESH :
- geographic , epidemiology : Germany.
- classification : Viruses.
- diagnosis : Respiratory Tract Infections.
- epidemiology : Respiratory Tract Infections.
- genetics : Viruses.
- isolation & purification : Viruses.
- methods : Molecular Diagnostic Techniques, Multiplex Polymerase Chain Reaction, Real-Time Polymerase Chain Reaction, Virology.
- virology : Respiratory Tract Infections.
- Adolescent, Adult, Child, Child, Preschool, Female, Humans, Infant, Male, Middle Aged, Prevalence, Prospective Studies, Sensitivity and Specificity, Young Adult.
Abstract
The aim of this study was to determine the prevalence of respiratory viruses and to prospectively evaluate the performance of the fast-track diagnostics (FTD) respiratory pathogens multiplex PCR assay shortly after the 2009/10 influenza pandemic. Highly sensitive monoplex real-time PCR assays served as references. Discrepant results were further analyzed by the xTAG RVP Fast assay. A total of 369 respiratory samples from children and adults were collected prospectively in Germany from December 2009 until June 2010. The sensitivity and specificity of the FTD assay after resolution of discrepant results was 92.2 % and 99.5 %, respectively. Lowest specificity of the FTD assay was observed for human bocavirus. Multiple detections were recorded in 33/369 (8.9 %) of the samples by monoplex PCR and in 43/369 (11.7 %) using the FTD assay. The most prevalent viruses were respiratory syncytial virus and human metapneumovirus. Only pandemic influenza virus A/H1N1 (2009), and not seasonal influenza virus, was detected. Viruses other than influenza virus accounted for the majority of acute respiratory infections. The FTD assay can be easily implemented in general diagnostic laboratories and facilitate the optimization of patient-management schemes.
Url:
DOI: 10.1007/s00705-013-1876-3
PubMed: 24126621
PubMed Central: 7086993
Affiliations:
- Allemagne, Pays-Bas
- Bade-Wurtemberg, District de Dresde, District de Fribourg-en-Brisgau, Saxe (Land)
- Dresde, Fribourg-en-Brisgau
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Le document en format XML
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polymérisation en chaine en temps réel ()</term><term>Réaction de polymérisation en chaine multiplex ()</term><term>Sensibilité et spécificité</term><term>Techniques de diagnostic moléculaire ()</term><term>Virologie ()</term><term>Virus ()</term><term>Virus (génétique)</term><term>Virus (isolement et purification)</term><term>Études prospectives</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Germany</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Respiratory Tract Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections de l'appareil respiratoire</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Respiratory Tract Infections</term></keywords><keywords scheme="MESH" qualifier="genetics" 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xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Child</term><term>Child, Preschool</term><term>Female</term><term>Humans</term><term>Infant</term><term>Male</term><term>Middle Aged</term><term>Prevalence</term><term>Prospective Studies</term><term>Sensitivity and Specificity</term><term>Young Adult</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Humains</term><term>Jeune adulte</term><term>Mâle</term><term>Nourrisson</term><term>Prévalence</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Réaction de polymérisation en chaine multiplex</term><term>Sensibilité et spécificité</term><term>Techniques de diagnostic moléculaire</term><term>Virologie</term><term>Virus</term><term>Études prospectives</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Allemagne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The aim of this study was to determine the prevalence of respiratory viruses and to prospectively evaluate the performance of the fast-track diagnostics (FTD) respiratory pathogens multiplex PCR assay shortly after the 2009/10 influenza pandemic. Highly sensitive monoplex real-time PCR assays served as references. Discrepant results were further analyzed by the xTAG RVP Fast assay. A total of 369 respiratory samples from children and adults were collected prospectively in Germany from December 2009 until June 2010. The sensitivity and specificity of the FTD assay after resolution of discrepant results was 92.2 % and 99.5 %, respectively. Lowest specificity of the FTD assay was observed for human bocavirus. Multiple detections were recorded in 33/369 (8.9 %) of the samples by monoplex PCR and in 43/369 (11.7 %) using the FTD assay. The most prevalent viruses were respiratory syncytial virus and human metapneumovirus. Only pandemic influenza virus A/H1N1 (2009), and not seasonal influenza virus, was detected. Viruses other than influenza virus accounted for the majority of acute respiratory infections. The FTD assay can be easily implemented in general diagnostic laboratories and facilitate the optimization of patient-management schemes.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Anderson, Tp" uniqKey="Anderson T">TP Anderson</name></author><author><name sortKey="Werno, Am" uniqKey="Werno A">AM Werno</name></author><author><name sortKey="Barratt, K" uniqKey="Barratt K">K Barratt</name></author><author><name sortKey="Mahagamasekera, P" uniqKey="Mahagamasekera P">P Mahagamasekera</name></author><author><name sortKey="Murdoch, Dr" uniqKey="Murdoch D">DR Murdoch</name></author><author><name sortKey="Jennings, Lc" uniqKey="Jennings L">LC Jennings</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bierbaum, S" uniqKey="Bierbaum S">S Bierbaum</name></author><author><name sortKey="Konigsfeld, N" uniqKey="Konigsfeld N">N Konigsfeld</name></author><author><name sortKey="Besazza, N" uniqKey="Besazza 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last="Berner">Reinhard Berner</name><name sortKey="Berner, Reinhard" sort="Berner, Reinhard" uniqKey="Berner R" first="Reinhard" last="Berner">Reinhard Berner</name><name sortKey="Forster, Johannes" sort="Forster, Johannes" uniqKey="Forster J" first="Johannes" last="Forster">Johannes Forster</name><name sortKey="Neumann Haefelin, Dieter" sort="Neumann Haefelin, Dieter" uniqKey="Neumann Haefelin D" first="Dieter" last="Neumann-Haefelin">Dieter Neumann-Haefelin</name><name sortKey="Panning, Marcus" sort="Panning, Marcus" uniqKey="Panning M" first="Marcus" last="Panning">Marcus Panning</name><name sortKey="Rucker, Gerta" sort="Rucker, Gerta" uniqKey="Rucker G" first="Gerta" last="Rücker">Gerta Rücker</name></country><country name="Pays-Bas"><noRegion><name sortKey="Rohde, Gernot" sort="Rohde, Gernot" uniqKey="Rohde G" first="Gernot" last="Rohde">Gernot Rohde</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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