PandemieGrippaleV1, Main, Exploration, bibRecord, 001121

Characterization and immunogenicity in mice of recombinant influenza haemagglutinins produced in Leishmania tarentolae

Identifieur interne : 001121 ( Main/Exploration ); précédent : 001120; suivant : 001122

Characterization and immunogenicity in mice of recombinant influenza haemagglutinins produced in Leishmania tarentolae

Auteurs : Corinne Pion [France] ; Virginie Courtois [France] ; Stéphanie Husson [France] ; Marie-Clotilde Bernard [France] ; Marie-Claire Nicolai [France] ; Philippe Talaga [France] ; Emanuelle Trannoy [France] ; Catherine Moste [France] ; Régis Sodoyer [France] ; Isabelle Legastelois [France]

Source :

Vaccine [ 0264-410X ] ; 2014.

RBID : Pascal:14-0250685

Descripteurs français

Pascal (Inist)
- Souris, Leishmania tarentolae, Immunogénicité, Hémagglutinine, Grippe.

English descriptors

KwdEn :
- Hemagglutinin, Immunogenicity, Influenza, Leishmania tarentolae, Mouse.

Abstract

The membrane displayed antigen haemagglutinin (HA) from several influenza strains were expressed in the Leishmania tarentolae system. This non-conventional expression system based on a parasite of lizards, can be readily propagated to high cell density (>10⁸ cells/mL) in a simple incubator at 26°C. The genes encoding HA proteins were cloned from six influenza strains, among these being a 2009 A/H1N1 pandemic strain from swine origin, namely A/California/07/09(H1N1). Soluble HA proteins were secreted into the cell culture medium and were easily and successfully purified via a His-Tag domain fused to the proteins. The overall process could be conducted in less than 3 months and resulted in a yield of approximately 1.5-5 mg of HA per liter of biofermenter culture after purification. The recombinant HA proteins expressed by L. tarentolae were characterized by dynamic light scattering and were observed to be mostly monomeric. The L. tarentolae recombinant HA proteins were immunogenic in mice at a dose of 10 μg when administered twice with an oil-in-water emulsion-based adjuvant. These results suggest that the L. tarentolae expression system may be an alternative to the current egg-based vaccine production.

Affiliations:

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
<settlement type="city">Marcy L'Etoile</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Technology Research Institute Bioaster, 317 Avenue Jean-Jaurès</s1>
<s2>69007 Lyon</s2>
<s3>FRA</s3>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Legastelois, Isabelle" sort="Legastelois, Isabelle" uniqKey="Legastelois I" first="Isabelle" last="Legastelois">Isabelle Legastelois</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Research and Development, Sanofi Pasteur, 1541 Avenue Marcel Mérieux</s1>
<s2>69280 Marcy L'Etoile</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Rhône-Alpes</region>
<settlement type="city">Marcy L'Etoile</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Vaccine</title>
<title level="j" type="abbreviated">Vaccine</title>
<idno type="ISSN">0264-410X</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Vaccine</title>
<title level="j" type="abbreviated">Vaccine</title>
<idno type="ISSN">0264-410X</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Hemagglutinin</term>
<term>Immunogenicity</term>
<term>Influenza</term>
<term>Leishmania tarentolae</term>
<term>Mouse</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Souris</term>
<term>Leishmania tarentolae</term>
<term>Immunogénicité</term>
<term>Hémagglutinine</term>
<term>Grippe</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The membrane displayed antigen haemagglutinin (HA) from several influenza strains were expressed in the Leishmania tarentolae system. This non-conventional expression system based on a parasite of lizards, can be readily propagated to high cell density (>10<sup>8</sup>
 cells/mL) in a simple incubator at 26°C. The genes encoding HA proteins were cloned from six influenza strains, among these being a 2009 A/H1N1 pandemic strain from swine origin, namely A/California/07/09(H1N1). Soluble HA proteins were secreted into the cell culture medium and were easily and successfully purified via a His-Tag domain fused to the proteins. The overall process could be conducted in less than 3 months and resulted in a yield of approximately 1.5-5 mg of HA per liter of biofermenter culture after purification. The recombinant HA proteins expressed by L. tarentolae were characterized by dynamic light scattering and were observed to be mostly monomeric. The L. tarentolae recombinant HA proteins were immunogenic in mice at a dose of 10 μg when administered twice with an oil-in-water emulsion-based adjuvant. These results suggest that the L. tarentolae expression system may be an alternative to the current egg-based vaccine production.</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
</country>
<region><li>Auvergne-Rhône-Alpes</li>
<li>Rhône-Alpes</li>
</region>
<settlement><li>Lyon</li>
<li>Marcy L'Etoile</li>
</settlement>
</list>
<tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Pion, Corinne" sort="Pion, Corinne" uniqKey="Pion C" first="Corinne" last="Pion">Corinne Pion</name>
</region>
<name sortKey="Bernard, Marie Clotilde" sort="Bernard, Marie Clotilde" uniqKey="Bernard M" first="Marie-Clotilde" last="Bernard">Marie-Clotilde Bernard</name>
<name sortKey="Courtois, Virginie" sort="Courtois, Virginie" uniqKey="Courtois V" first="Virginie" last="Courtois">Virginie Courtois</name>
<name sortKey="Husson, Stephanie" sort="Husson, Stephanie" uniqKey="Husson S" first="Stéphanie" last="Husson">Stéphanie Husson</name>
<name sortKey="Legastelois, Isabelle" sort="Legastelois, Isabelle" uniqKey="Legastelois I" first="Isabelle" last="Legastelois">Isabelle Legastelois</name>
<name sortKey="Moste, Catherine" sort="Moste, Catherine" uniqKey="Moste C" first="Catherine" last="Moste">Catherine Moste</name>
<name sortKey="Nicolai, Marie Claire" sort="Nicolai, Marie Claire" uniqKey="Nicolai M" first="Marie-Claire" last="Nicolai">Marie-Claire Nicolai</name>
<name sortKey="Sodoyer, Regis" sort="Sodoyer, Regis" uniqKey="Sodoyer R" first="Régis" last="Sodoyer">Régis Sodoyer</name>
<name sortKey="Sodoyer, Regis" sort="Sodoyer, Regis" uniqKey="Sodoyer R" first="Régis" last="Sodoyer">Régis Sodoyer</name>
<name sortKey="Talaga, Philippe" sort="Talaga, Philippe" uniqKey="Talaga P" first="Philippe" last="Talaga">Philippe Talaga</name>
<name sortKey="Trannoy, Emanuelle" sort="Trannoy, Emanuelle" uniqKey="Trannoy E" first="Emanuelle" last="Trannoy">Emanuelle Trannoy</name>
</country>
</tree>
</affiliations>
</record>

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Characterization and immunogenicity in mice of recombinant influenza haemagglutinins produced in Leishmania tarentolae

Characterization and immunogenicity in mice of recombinant influenza haemagglutinins produced in Leishmania tarentolae

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