PandemieGrippaleV1, Main, Curation, bibRecord, 004B99

Rebuilding microbial genomes

Identifieur interne : 004B99 ( Main/Curation ); précédent : 004B98; suivant : 004C00

Rebuilding microbial genomes

Auteurs : Robert A. Holt [Canada] ; Rene Warren [Canada] ; Stephane Flibotte [Canada] ; Perseus I. Missirlis [Canada] ; Duane E. Smailus [Canada]

Source :

BioEssays [ 0265-9247 ] ; 2007-06.

RBID : ISTEX:6FDFE0B8826F066DE4B55591C2C196674968813B

English descriptors

Teeft :
- Acad, Bioessays, Biol chem, Bipartite genome, British columbia, Cell hydrolase, Chromosome, Clone, Coli, Coli host, Donor, Donor chromosome, Donor genome, Escherichia, Escherichia coli, Essential genes, Gene, Genetic material, Genome, Genome assembly, Genome resolution, Genomic, Haemophilus, Haemophilus influenzae, Host cell, Host chromosome, Host genome, Host organism, Hydrolase, Influenzae, Influenzae chromosome, Influenzae genes, Influenzae genome, Large segments, Lethal genes, Microbial, Microbial genome, Minimal tiling, Model system, Natl, Plasmid, Proc, Proc natl acad, Recombination, Replication, Ribosomal, Ribosomal protein, Ribosomal proteins, Rnase, Synthetase, Synthetic genome, Toxic, Toxic genes, Toxicity, Transporter.

Abstract

Engineered microbes are of great potential utility in biotechnology and basic research. In principle, a cell can be built from scratch by assembling small molecule sets with auto‐catalytic properties. Alternatively, DNA can be isolated or directly synthesized and molded into a synthetic genome using existing genomic blueprints and molecular biology tools. Activating such a synthetic genome will yield a synthetic cell. Here we examine obstacles associated with this latter approach using a model system whereby a donor genome from H. influenzae is fragmented, and the pieces are then modified and reassembled stepwise in an E. coli host cell. There are obstacles associated with this strategy related to DNA transfer, DNA replication, cross‐talk in gene regulation and compatibility of gene products between donor and host. Encouragingly, analysis of gene expression indicates widespread transcription of H. influenzae genes in E. coli, and analysis of gap locations in H. influenzae and other microbial genome assemblies reveals few genes routinely incompatible with E. coli. In conclusion, rebuilding and booting a genome remains a feasible and pragmatic approach to creating a synthetic microbial cell. BioEssays 29:580–590, 2007. © 2007 Wiley Periodicals, Inc.

Url:

https://api.istex.fr/ark:/67375/WNG-C2V7CZB2-6/fulltext.pdf

DOI: 10.1002/bies.20585

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Le document en format XML

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<author><name sortKey="Flibotte, Stephane" sort="Flibotte, Stephane" uniqKey="Flibotte S" first="Stephane" last="Flibotte">Stephane Flibotte</name>
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<term>Cell hydrolase</term>
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<term>Clone</term>
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<term>Host chromosome</term>
<term>Host genome</term>
<term>Host organism</term>
<term>Hydrolase</term>
<term>Influenzae</term>
<term>Influenzae chromosome</term>
<term>Influenzae genes</term>
<term>Influenzae genome</term>
<term>Large segments</term>
<term>Lethal genes</term>
<term>Microbial</term>
<term>Microbial genome</term>
<term>Minimal tiling</term>
<term>Model system</term>
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<term>Plasmid</term>
<term>Proc</term>
<term>Proc natl acad</term>
<term>Recombination</term>
<term>Replication</term>
<term>Ribosomal</term>
<term>Ribosomal protein</term>
<term>Ribosomal proteins</term>
<term>Rnase</term>
<term>Synthetase</term>
<term>Synthetic genome</term>
<term>Toxic</term>
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<front><div type="abstract" xml:lang="en">Engineered microbes are of great potential utility in biotechnology and basic research. In principle, a cell can be built from scratch by assembling small molecule sets with auto‐catalytic properties. Alternatively, DNA can be isolated or directly synthesized and molded into a synthetic genome using existing genomic blueprints and molecular biology tools. Activating such a synthetic genome will yield a synthetic cell. Here we examine obstacles associated with this latter approach using a model system whereby a donor genome from H. influenzae is fragmented, and the pieces are then modified and reassembled stepwise in an E. coli host cell. There are obstacles associated with this strategy related to DNA transfer, DNA replication, cross‐talk in gene regulation and compatibility of gene products between donor and host. Encouragingly, analysis of gene expression indicates widespread transcription of H. influenzae genes in E. coli, and analysis of gap locations in H. influenzae and other microbial genome assemblies reveals few genes routinely incompatible with E. coli. In conclusion, rebuilding and booting a genome remains a feasible and pragmatic approach to creating a synthetic microbial cell. BioEssays 29:580–590, 2007. © 2007 Wiley Periodicals, Inc.</div>
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Rebuilding microbial genomes

Rebuilding microbial genomes

Source :

English descriptors

Abstract

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