Microneedle delivery of an M2e-TLR5 ligand fusion protein to skin confers broadly cross-protective influenza immunity
Identifieur interne : 000E28 ( Main/Curation ); précédent : 000E27; suivant : 000E29Microneedle delivery of an M2e-TLR5 ligand fusion protein to skin confers broadly cross-protective influenza immunity
Auteurs : Bao-Zhong Wang [États-Unis] ; Harvinder S. Gill [États-Unis] ; Cheng He [République populaire de Chine] ; Changbo Ou [République populaire de Chine] ; Li Wang [États-Unis] ; Ying-Chun Wang [États-Unis] ; Hao Feng [États-Unis] ; Han Zhang [États-Unis] ; Mark R. Prausnitz [États-Unis] ; Richard W. Compans [États-Unis]Source :
- Journal of controlled release : official journal of the Controlled Release Society [ 0168-3659 ] ; 2014.
Descripteurs français
- KwdFr :
- Administration par voie cutanée, Aiguilles, Animaux, Cellules rénales canines Madin-Darby, Chiens, Flagelline (administration et posologie), Flagelline (immunologie), Immunité, Immunoglobuline G (immunologie), Immunoglobuline G (sang), Infections à Orthomyxoviridae (), Infections à Orthomyxoviridae (immunologie), Infections à Orthomyxoviridae (sang), Infections à Orthomyxoviridae (virologie), Ligands, Lignée cellulaire, Microinjections, Protéines de fusion recombinantes (administration et posologie), Protéines de fusion recombinantes (immunologie), Protéines de la matrice virale (administration et posologie), Protéines de la matrice virale (immunologie), Récepteur de type Toll-5 (), Récepteur de type Toll-5 (immunologie), Souris, Sous-type H1N1 du virus de la grippe A (immunologie), Sous-type H3N2 du virus de la grippe A (immunologie), Vaccins antigrippaux (administration et posologie).
- MESH :
- administration et posologie : Flagelline, Protéines de fusion recombinantes, Protéines de la matrice virale, Vaccins antigrippaux.
- immunologie : Flagelline, Immunoglobuline G, Infections à Orthomyxoviridae, Protéines de fusion recombinantes, Protéines de la matrice virale, Récepteur de type Toll-5, Sous-type H1N1 du virus de la grippe A, Sous-type H3N2 du virus de la grippe A.
- sang : Immunoglobuline G, Infections à Orthomyxoviridae.
- virologie : Infections à Orthomyxoviridae.
- Administration par voie cutanée, Aiguilles, Animaux, Cellules rénales canines Madin-Darby, Chiens, Immunité, Infections à Orthomyxoviridae, Ligands, Lignée cellulaire, Microinjections, Récepteur de type Toll-5, Souris.
English descriptors
- KwdEn :
- Administration, Cutaneous, Animals, Cell Line, Dogs, Flagellin (administration & dosage), Flagellin (immunology), Immunity, Immunoglobulin G (blood), Immunoglobulin G (immunology), Influenza A Virus, H1N1 Subtype (immunology), Influenza A Virus, H3N2 Subtype (immunology), Influenza Vaccines (administration & dosage), Ligands, Madin Darby Canine Kidney Cells, Mice, Microinjections, Needles, Orthomyxoviridae Infections (blood), Orthomyxoviridae Infections (immunology), Orthomyxoviridae Infections (prevention & control), Orthomyxoviridae Infections (virology), Recombinant Fusion Proteins (administration & dosage), Recombinant Fusion Proteins (immunology), Toll-Like Receptor 5 (chemistry), Toll-Like Receptor 5 (immunology), Viral Matrix Proteins (administration & dosage), Viral Matrix Proteins (immunology).
- MESH :
- chemical , administration & dosage : Flagellin, Influenza Vaccines, Recombinant Fusion Proteins, Viral Matrix Proteins.
- chemical , blood : Immunoglobulin G.
- chemical , chemistry : Toll-Like Receptor 5.
- chemical , immunology : Flagellin, Immunoglobulin G, Recombinant Fusion Proteins, Toll-Like Receptor 5, Viral Matrix Proteins.
- blood : Orthomyxoviridae Infections.
- immunology : Influenza A Virus, H1N1 Subtype, Influenza A Virus, H3N2 Subtype, Orthomyxoviridae Infections.
- prevention & control : Orthomyxoviridae Infections.
- virology : Orthomyxoviridae Infections.
- Administration, Cutaneous, Animals, Cell Line, Dogs, Immunity, Ligands, Madin Darby Canine Kidney Cells, Mice, Microinjections, Needles.
Abstract
Influenza vaccines with broad cross-protection are urgently needed to prevent an emerging influenza pandemic. A fusion protein of the TLR5-agonist domains from flagellin and multiple repeats of the conserved extracellular domain of the influenza matrix protein 2 (M2e) was constructed, purified and evaluated as such a vaccine. A painless vaccination method suitable for possible self-administration using coated microneedle arrays was investigated for skin-targeted delivery of the fusion protein in a mouse model. The results demonstrate that microneedle immunization induced strong humoral as well as mucosal antibody responses and conferred complete protection against homo- and heterosubtypic lethal virus challenges. Protective efficacy with microneedles was found to be significantly better than that seen with conventional intramuscular injection, and comparable to that observed with intranasal immunization. Because of its advantages for administration, safety and storage, microneedle delivery of M2e-flagellin fusion protein is a promising approach for an easy-to-administer universal influenza vaccine.
Url:
DOI: 10.1016/j.jconrel.2014.01.002
PubMed: 24417966
PubMed Central: 3951682
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PMC:3951682Le document en format XML
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Administration, Cutaneous</term>
<term>Animals</term>
<term>Cell Line</term>
<term>Dogs</term>
<term>Flagellin (administration & dosage)</term>
<term>Flagellin (immunology)</term>
<term>Immunity</term>
<term>Immunoglobulin G (blood)</term>
<term>Immunoglobulin G (immunology)</term>
<term>Influenza A Virus, H1N1 Subtype (immunology)</term>
<term>Influenza A Virus, H3N2 Subtype (immunology)</term>
<term>Influenza Vaccines (administration & dosage)</term>
<term>Ligands</term>
<term>Madin Darby Canine Kidney Cells</term>
<term>Mice</term>
<term>Microinjections</term>
<term>Needles</term>
<term>Orthomyxoviridae Infections (blood)</term>
<term>Orthomyxoviridae Infections (immunology)</term>
<term>Orthomyxoviridae Infections (prevention & control)</term>
<term>Orthomyxoviridae Infections (virology)</term>
<term>Recombinant Fusion Proteins (administration & dosage)</term>
<term>Recombinant Fusion Proteins (immunology)</term>
<term>Toll-Like Receptor 5 (chemistry)</term>
<term>Toll-Like Receptor 5 (immunology)</term>
<term>Viral Matrix Proteins (administration & dosage)</term>
<term>Viral Matrix Proteins (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Administration par voie cutanée</term>
<term>Aiguilles</term>
<term>Animaux</term>
<term>Cellules rénales canines Madin-Darby</term>
<term>Chiens</term>
<term>Flagelline (administration et posologie)</term>
<term>Flagelline (immunologie)</term>
<term>Immunité</term>
<term>Immunoglobuline G (immunologie)</term>
<term>Immunoglobuline G (sang)</term>
<term>Infections à Orthomyxoviridae ()</term>
<term>Infections à Orthomyxoviridae (immunologie)</term>
<term>Infections à Orthomyxoviridae (sang)</term>
<term>Infections à Orthomyxoviridae (virologie)</term>
<term>Ligands</term>
<term>Lignée cellulaire</term>
<term>Microinjections</term>
<term>Protéines de fusion recombinantes (administration et posologie)</term>
<term>Protéines de fusion recombinantes (immunologie)</term>
<term>Protéines de la matrice virale (administration et posologie)</term>
<term>Protéines de la matrice virale (immunologie)</term>
<term>Récepteur de type Toll-5 ()</term>
<term>Récepteur de type Toll-5 (immunologie)</term>
<term>Souris</term>
<term>Sous-type H1N1 du virus de la grippe A (immunologie)</term>
<term>Sous-type H3N2 du virus de la grippe A (immunologie)</term>
<term>Vaccins antigrippaux (administration et posologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Flagellin</term>
<term>Influenza Vaccines</term>
<term>Recombinant Fusion Proteins</term>
<term>Viral Matrix Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Immunoglobulin G</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Toll-Like Receptor 5</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Flagellin</term>
<term>Immunoglobulin G</term>
<term>Recombinant Fusion Proteins</term>
<term>Toll-Like Receptor 5</term>
<term>Viral Matrix Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Flagelline</term>
<term>Protéines de fusion recombinantes</term>
<term>Protéines de la matrice virale</term>
<term>Vaccins antigrippaux</term>
</keywords>
<keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Orthomyxoviridae Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Flagelline</term>
<term>Immunoglobuline G</term>
<term>Infections à Orthomyxoviridae</term>
<term>Protéines de fusion recombinantes</term>
<term>Protéines de la matrice virale</term>
<term>Récepteur de type Toll-5</term>
<term>Sous-type H1N1 du virus de la grippe A</term>
<term>Sous-type H3N2 du virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term>
<term>Influenza A Virus, H3N2 Subtype</term>
<term>Orthomyxoviridae Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Orthomyxoviridae Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Immunoglobuline G</term>
<term>Infections à Orthomyxoviridae</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à Orthomyxoviridae</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Orthomyxoviridae Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Administration, Cutaneous</term>
<term>Animals</term>
<term>Cell Line</term>
<term>Dogs</term>
<term>Immunity</term>
<term>Ligands</term>
<term>Madin Darby Canine Kidney Cells</term>
<term>Mice</term>
<term>Microinjections</term>
<term>Needles</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Administration par voie cutanée</term>
<term>Aiguilles</term>
<term>Animaux</term>
<term>Cellules rénales canines Madin-Darby</term>
<term>Chiens</term>
<term>Immunité</term>
<term>Infections à Orthomyxoviridae</term>
<term>Ligands</term>
<term>Lignée cellulaire</term>
<term>Microinjections</term>
<term>Récepteur de type Toll-5</term>
<term>Souris</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="P1">Influenza vaccines with broad cross-protection are urgently needed to prevent an emerging influenza pandemic. A fusion protein of the TLR5-agonist domains from flagellin and multiple repeats of the conserved extracellular domain of the influenza matrix protein 2 (M2e) was constructed, purified and evaluated as such a vaccine. A painless vaccination method suitable for possible self-administration using coated microneedle arrays was investigated for skin-targeted delivery of the fusion protein in a mouse model. The results demonstrate that microneedle immunization induced strong humoral as well as mucosal antibody responses and conferred complete protection against homo- and heterosubtypic lethal virus challenges. Protective efficacy with microneedles was found to be significantly better than that seen with conventional intramuscular injection, and comparable to that observed with intranasal immunization. Because of its advantages for administration, safety and storage, microneedle delivery of M2e-flagellin fusion protein is a promising approach for an easy-to-administer universal influenza vaccine.</p>
</div>
</front>
</TEI>
</record>
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