Immuno-interferometric sensor for the detection of influenza A nucleoprotein
Identifieur interne : 001296 ( Istex/Corpus ); précédent : 001295; suivant : 001297Immuno-interferometric sensor for the detection of influenza A nucleoprotein
Auteurs : Leslie R. Farris ; Nan Wu ; Wenhui Wang ; Lisa-Jo A. Clarizia ; Xingwei Wang ; Melisenda J. McdonaldSource :
- Analytical and Bioanalytical Chemistry [ 1618-2642 ] ; 2010.
Abstract
Abstract: Influenza A virus, both seasonal and pandemic, has the potential to cause rampant devastating disease around the world. The most relied upon methods of viral detection require days, skilled workers, and laboratory settings to complete properly. Here, we report two methods for the detection of the nucleoprotein from inactivated influenza A (IFA-NP), a patented polymer–protein antibody orientation immuno-method, termed ALYNGSA, and a newly fabricated optical label-free Fabry–Perot interferometric immunosensor. The ALYGNSA assay for IFA-NP had a level of detection below 5 μg/mL of inactivated virus sample. The label-free detection through Fabry–Perot interferometry with the ALYGNSA orientation yielded an improved sensitivity to 1 μg/mL over the fluorescence sandwich assay alone. Characterization of the detection surface by fluorescence microscopy and non-contact AFM corroborated interferometry results. The resulting label-free detection method has the prospective for adaptation into a portable multi-chip sensor capable of real-time in situ detection of influenza A virus.
Url:
DOI: 10.1007/s00216-009-3235-5
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Farris</name><affiliation><mods:affiliation>Department of Chemistry, University of Massachusetts, One University Ave., 01854, Lowell, MA, USA</mods:affiliation></affiliation></author><author><name sortKey="Wu, Nan" sort="Wu, Nan" uniqKey="Wu N" first="Nan" last="Wu">Nan Wu</name><affiliation><mods:affiliation>Department of Electrical and Computer Engineering, University of Massachusetts, One University Ave., 01854, Lowell, MA, USA</mods:affiliation></affiliation></author><author><name sortKey="Wang, Wenhui" sort="Wang, Wenhui" uniqKey="Wang W" first="Wenhui" last="Wang">Wenhui Wang</name><affiliation><mods:affiliation>Department of Electrical and Computer Engineering, University of Massachusetts, One University Ave., 01854, Lowell, MA, USA</mods:affiliation></affiliation></author><author><name sortKey="Clarizia, Lisa Jo A" sort="Clarizia, Lisa Jo A" uniqKey="Clarizia L" first="Lisa-Jo A." last="Clarizia">Lisa-Jo A. 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The most relied upon methods of viral detection require days, skilled workers, and laboratory settings to complete properly. Here, we report two methods for the detection of the nucleoprotein from inactivated influenza A (IFA-NP), a patented polymer–protein antibody orientation immuno-method, termed ALYNGSA, and a newly fabricated optical label-free Fabry–Perot interferometric immunosensor. The ALYGNSA assay for IFA-NP had a level of detection below 5 μg/mL of inactivated virus sample. The label-free detection through Fabry–Perot interferometry with the ALYGNSA orientation yielded an improved sensitivity to 1 μg/mL over the fluorescence sandwich assay alone. Characterization of the detection surface by fluorescence microscopy and non-contact AFM corroborated interferometry results. The resulting label-free detection method has the prospective for adaptation into a portable multi-chip sensor capable of real-time in situ detection of influenza A virus.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Leslie R. Farris</name><affiliations><json:string>Department of Chemistry, University of Massachusetts, One University Ave., 01854, Lowell, MA, USA</json:string></affiliations></json:item><json:item><name>Nan Wu</name><affiliations><json:string>Department of Electrical and Computer Engineering, University of Massachusetts, One University Ave., 01854, Lowell, MA, USA</json:string></affiliations></json:item><json:item><name>Wenhui Wang</name><affiliations><json:string>Department of Electrical and Computer Engineering, University of Massachusetts, One University Ave., 01854, Lowell, MA, USA</json:string></affiliations></json:item><json:item><name>Lisa-Jo A. 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DisplayOrder="Western"><GivenName>Lisa-Jo</GivenName><GivenName>A.</GivenName><FamilyName>Clarizia</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Xingwei</GivenName><FamilyName>Wang</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Melisenda</GivenName><GivenName>J.</GivenName><FamilyName>McDonald</FamilyName></AuthorName><Contact><Email>Melisenda_McDonald@uml.edu</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>Department of Chemistry</OrgDivision><OrgName>University of Massachusetts</OrgName><OrgAddress><Street>One University Ave.</Street><City>Lowell</City><State>MA</State><Postcode>01854</Postcode><Country Code="US">USA</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Department of Electrical and Computer Engineering</OrgDivision><OrgName>University of Massachusetts</OrgName><OrgAddress><Street>One University Ave.</Street><City>Lowell</City><State>MA</State><Postcode>01854</Postcode><Country Code="US">USA</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En" OutputMedium="All"><Heading>Abstract</Heading><Para>Influenza A virus, both seasonal and pandemic, has the potential to cause rampant devastating disease around the world. The most relied upon methods of viral detection require days, skilled workers, and laboratory settings to complete properly. Here, we report two methods for the detection of the nucleoprotein from inactivated influenza A (IFA-NP), a patented polymer–protein antibody orientation immuno-method, termed ALYNGSA, and a newly fabricated optical label-free Fabry–Perot interferometric immunosensor. The ALYGNSA assay for IFA-NP had a level of detection below 5 μg/mL of inactivated virus sample. The label-free detection through Fabry–Perot interferometry with the ALYGNSA orientation yielded an improved sensitivity to 1 μg/mL over the fluorescence sandwich assay alone. Characterization of the detection surface by fluorescence microscopy and non-contact AFM corroborated interferometry results. The resulting label-free detection method has the prospective for adaptation into a portable multi-chip sensor capable of real-time in situ detection of influenza A virus.</Para></Abstract><KeywordGroup Language="En" OutputMedium="All"><Heading>Keywords</Heading><Keyword>Influenza A nucleoprotein</Keyword><Keyword>Biosensor</Keyword><Keyword>Immunoassay</Keyword><Keyword>Interferometry</Keyword><Keyword>Laser scanning confocal microscopy</Keyword><Keyword>Atomic force microscopy</Keyword><Keyword>Optical sensors</Keyword><Keyword>Immunoassays/ELISA</Keyword><Keyword>Influenza A</Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Immuno-interferometric sensor for the detection of influenza A nucleoprotein</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Immuno-interferometric sensor for the detection of influenza A nucleoprotein</title></titleInfo><name type="personal"><namePart type="given">Leslie</namePart><namePart type="given">R.</namePart><namePart type="family">Farris</namePart><affiliation>Department of Chemistry, University of Massachusetts, One University Ave., 01854, Lowell, MA, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Nan</namePart><namePart type="family">Wu</namePart><affiliation>Department of Electrical and Computer Engineering, University of Massachusetts, One University Ave., 01854, Lowell, MA, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Wenhui</namePart><namePart type="family">Wang</namePart><affiliation>Department of Electrical and Computer Engineering, University of Massachusetts, One University Ave., 01854, Lowell, MA, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Lisa-Jo</namePart><namePart type="given">A.</namePart><namePart type="family">Clarizia</namePart><affiliation>Department of Chemistry, University of Massachusetts, One University Ave., 01854, Lowell, MA, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Xingwei</namePart><namePart type="family">Wang</namePart><affiliation>Department of Electrical and Computer Engineering, University of Massachusetts, One University Ave., 01854, Lowell, MA, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Melisenda</namePart><namePart type="given">J.</namePart><namePart type="family">McDonald</namePart><affiliation>Department of Chemistry, University of Massachusetts, One University Ave., 01854, Lowell, MA, USA</affiliation><affiliation>E-mail: Melisenda_McDonald@uml.edu</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Berlin/Heidelberg</placeTerm></place><dateCreated encoding="w3cdtf">2009-08-21</dateCreated><dateIssued encoding="w3cdtf">2010-01-01</dateIssued><copyrightDate encoding="w3cdtf">2009</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Influenza A virus, both seasonal and pandemic, has the potential to cause rampant devastating disease around the world. The most relied upon methods of viral detection require days, skilled workers, and laboratory settings to complete properly. Here, we report two methods for the detection of the nucleoprotein from inactivated influenza A (IFA-NP), a patented polymer–protein antibody orientation immuno-method, termed ALYNGSA, and a newly fabricated optical label-free Fabry–Perot interferometric immunosensor. The ALYGNSA assay for IFA-NP had a level of detection below 5 μg/mL of inactivated virus sample. The label-free detection through Fabry–Perot interferometry with the ALYGNSA orientation yielded an improved sensitivity to 1 μg/mL over the fluorescence sandwich assay alone. Characterization of the detection surface by fluorescence microscopy and non-contact AFM corroborated interferometry results. The resulting label-free detection method has the prospective for adaptation into a portable multi-chip sensor capable of real-time in situ detection of influenza A virus.</abstract><note>Original Paper</note><subject lang="en"><genre>Keywords</genre><topic>Influenza A nucleoprotein</topic><topic>Biosensor</topic><topic>Immunoassay</topic><topic>Interferometry</topic><topic>Laser scanning confocal microscopy</topic><topic>Atomic force microscopy</topic><topic>Optical sensors</topic><topic>Immunoassays/ELISA</topic><topic>Influenza A</topic></subject><relatedItem type="host"><titleInfo><title>Analytical and Bioanalytical Chemistry</title></titleInfo><titleInfo type="abbreviated"><title>Anal Bioanal Chem</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2010</dateIssued><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><subject><genre>Chemistry</genre><topic>Ecotoxicology</topic><topic>Inorganic Chemistry</topic><topic>Food Science</topic><topic>Analytical Chemistry</topic><topic>Physical Chemistry</topic></subject><identifier type="ISSN">1618-2642</identifier><identifier type="eISSN">1618-2650</identifier><identifier type="JournalID">216</identifier><identifier type="IssueArticleCount">46</identifier><identifier type="VolumeIssueCount">8</identifier><part><date>2010</date><detail type="issue"><title>Marine Ecotoxicology</title></detail><detail type="volume"><number>396</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="pages"><start>667</start><end>674</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 2010</recordOrigin></recordInfo></relatedItem><identifier type="istex">9E66246BFCCF4FAF4EC1A65759873E95335FE04C</identifier><identifier type="ark">ark:/67375/VQC-V8P6NQX3-3</identifier><identifier type="DOI">10.1007/s00216-009-3235-5</identifier><identifier type="ArticleID">3235</identifier><identifier type="ArticleID">s00216-009-3235-5</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 2009</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Converted from (version 1.2.14) to MODS version 3.6.</recordOrigin><recordCreationDate encoding="w3cdtf">2020-05-30</recordCreationDate></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-V8P6NQX3-3/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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