Rapid discrimination of H5 and H9 subtypes of avian influenza viruses and Newcastle disease virus by multiplex RT-PCR
Identifieur interne : 000906 ( Istex/Corpus ); précédent : 000905; suivant : 000907Rapid discrimination of H5 and H9 subtypes of avian influenza viruses and Newcastle disease virus by multiplex RT-PCR
Auteurs : Hao-Tai Chen ; Jie Zhang ; De-Hui Sun ; Ji-Le Zhang ; Xue-Peng Cai ; Xiang-Tao Liu ; Yao-Zhong Ding ; Li-Na Ma ; Sheng-Hai Yang ; Lei Jin ; Yongsheng LiuSource :
- Veterinary Research Communications [ 0165-7380 ] ; 2008.
Abstract
Abstract: Avian influenza and Newcastle disease are the highly contagious and most economically important diseases in poultry industry throughout the world. A multiplex reverse transcription polymerase chain reaction (mRT-PCR) assay was developed for the rapid and specific discrimination of H5 and H9 subtypes of avian influenza viruses (AIV) and Newcastle disease virus (NDV). Three sets of specific primers were applied in the assay based on the sequences of the hemagglutinin gene of H5-AIV, H9-AIV and fusion protein gene of NDV. 59 clinical samples including the throat washes, oral swabs, and cloacal scrapings were detected by mRT-PCR and single RT-PCR (sRT-PCR), respectively. The results indicated that the sensitivity and specifity of mRT-PCR were in accordance with sRT-PCR. The mRT-PCR developed in this study may therefore provide a new avenue to rapid detection of these important pathogens in one reaction.
Url:
DOI: 10.1007/s11259-008-9052-z
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Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: haotaichen@tom.com</mods:affiliation></affiliation></author><author><name sortKey="Zhang, Jie" sort="Zhang, Jie" uniqKey="Zhang J" first="Jie" last="Zhang">Jie Zhang</name><affiliation><mods:affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</mods:affiliation></affiliation></author><author><name sortKey="Sun, De Hui" sort="Sun, De Hui" uniqKey="Sun D" first="De-Hui" last="Sun">De-Hui Sun</name><affiliation><mods:affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</mods:affiliation></affiliation></author><author><name sortKey="Zhang, Ji Le" sort="Zhang, Ji Le" uniqKey="Zhang J" first="Ji-Le" last="Zhang">Ji-Le Zhang</name><affiliation><mods:affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</mods:affiliation></affiliation></author><author><name sortKey="Cai, Xue Peng" sort="Cai, Xue Peng" uniqKey="Cai X" first="Xue-Peng" last="Cai">Xue-Peng Cai</name><affiliation><mods:affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</mods:affiliation></affiliation></author><author><name sortKey="Liu, Xiang Tao" sort="Liu, Xiang Tao" uniqKey="Liu X" first="Xiang-Tao" last="Liu">Xiang-Tao Liu</name><affiliation><mods:affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</mods:affiliation></affiliation></author><author><name sortKey="Ding, Yao Zhong" sort="Ding, Yao Zhong" uniqKey="Ding Y" first="Yao-Zhong" last="Ding">Yao-Zhong Ding</name><affiliation><mods:affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</mods:affiliation></affiliation></author><author><name sortKey="Ma, Li Na" sort="Ma, Li Na" uniqKey="Ma L" first="Li-Na" last="Ma">Li-Na Ma</name><affiliation><mods:affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</mods:affiliation></affiliation></author><author><name sortKey="Yang, Sheng Hai" sort="Yang, Sheng Hai" uniqKey="Yang S" first="Sheng-Hai" last="Yang">Sheng-Hai Yang</name><affiliation><mods:affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</mods:affiliation></affiliation></author><author><name sortKey="Jin, Lei" sort="Jin, Lei" uniqKey="Jin L" first="Lei" last="Jin">Lei Jin</name><affiliation><mods:affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</mods:affiliation></affiliation></author><author><name sortKey="Liu, Yongsheng" sort="Liu, Yongsheng" uniqKey="Liu Y" first="Yongsheng" last="Liu">Yongsheng Liu</name><affiliation><mods:affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: liuyongshengvip@sina.com.cn</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Veterinary Research Communications</title><title level="j" type="abbrev">Vet Res Commun</title><idno type="ISSN">0165-7380</idno><idno type="eISSN">1573-7446</idno><imprint><publisher ref="https://scientific-publisher.data.istex.fr/ark:/67375/H02-SWLMH5L1-1">Springer Netherlands</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="2008">2008</date><biblScope unit="vol" from="32" to="32">32</biblScope><biblScope unit="issue" from="6" to="6">6</biblScope><biblScope unit="page" from="491">491</biblScope><biblScope unit="page" to="498">498</biblScope></imprint><idno type="ISSN">0165-7380</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0165-7380</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Avian influenza and Newcastle disease are the highly contagious and most economically important diseases in poultry industry throughout the world. A multiplex reverse transcription polymerase chain reaction (mRT-PCR) assay was developed for the rapid and specific discrimination of H5 and H9 subtypes of avian influenza viruses (AIV) and Newcastle disease virus (NDV). Three sets of specific primers were applied in the assay based on the sequences of the hemagglutinin gene of H5-AIV, H9-AIV and fusion protein gene of NDV. 59 clinical samples including the throat washes, oral swabs, and cloacal scrapings were detected by mRT-PCR and single RT-PCR (sRT-PCR), respectively. The results indicated that the sensitivity and specifity of mRT-PCR were in accordance with sRT-PCR. The mRT-PCR developed in this study may therefore provide a new avenue to rapid detection of these important pathogens in one reaction.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Hao-tai Chen</name><affiliations><json:string>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</json:string><json:string>E-mail: haotaichen@tom.com</json:string></affiliations></json:item><json:item><name>Jie Zhang</name><affiliations><json:string>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</json:string></affiliations></json:item><json:item><name>De-hui Sun</name><affiliations><json:string>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</json:string></affiliations></json:item><json:item><name>Ji-le Zhang</name><affiliations><json:string>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</json:string></affiliations></json:item><json:item><name>Xue-peng Cai</name><affiliations><json:string>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</json:string></affiliations></json:item><json:item><name>Xiang-tao Liu</name><affiliations><json:string>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</json:string></affiliations></json:item><json:item><name>Yao-zhong Ding</name><affiliations><json:string>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</json:string></affiliations></json:item><json:item><name>Li-na Ma</name><affiliations><json:string>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</json:string></affiliations></json:item><json:item><name>Sheng-hai Yang</name><affiliations><json:string>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</json:string></affiliations></json:item><json:item><name>Lei Jin</name><affiliations><json:string>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</json:string></affiliations></json:item><json:item><name>Yongsheng Liu</name><affiliations><json:string>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</json:string><json:string>E-mail: liuyongshengvip@sina.com.cn</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Multiplex RT-PCR</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Avian influenza</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>H5 subtype</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>H9 subtype</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Newcastle disease</value></json:item></subject><articleId><json:string>9052</json:string><json:string>s11259-008-9052-z</json:string></articleId><arkIstex>ark:/67375/VQC-1LJNJKKP-Q</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: Avian influenza and Newcastle disease are the highly contagious and most economically important diseases in poultry industry throughout the world. A multiplex reverse transcription polymerase chain reaction (mRT-PCR) assay was developed for the rapid and specific discrimination of H5 and H9 subtypes of avian influenza viruses (AIV) and Newcastle disease virus (NDV). Three sets of specific primers were applied in the assay based on the sequences of the hemagglutinin gene of H5-AIV, H9-AIV and fusion protein gene of NDV. 59 clinical samples including the throat washes, oral swabs, and cloacal scrapings were detected by mRT-PCR and single RT-PCR (sRT-PCR), respectively. The results indicated that the sensitivity and specifity of mRT-PCR were in accordance with sRT-PCR. The mRT-PCR developed in this study may therefore provide a new avenue to rapid detection of these important pathogens in one reaction.</abstract><qualityIndicators><score>4.715</score><pdfWordCount>3059</pdfWordCount><pdfCharCount>18789</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>8</pdfPageCount><pdfPageSize>439.37 x 666.142 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>138</abstractWordCount><abstractCharCount>922</abstractCharCount><keywordCount>5</keywordCount><pdfWordsPerPage>382</pdfWordsPerPage><pdfText>true</pdfText></qualityIndicators><title>Rapid discrimination of H5 and H9 subtypes of avian influenza viruses and Newcastle disease virus by multiplex RT-PCR</title><genre><json:string>research-article</json:string></genre><host><title>Veterinary Research 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RT-PCR</title><author><persName><forename type="first">Hao-tai</forename><surname>Chen</surname></persName><email>haotaichen@tom.com</email><affiliation><orgName type="department">Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute</orgName><orgName type="institution">Chinese Academy of Agricultural Sciences</orgName><address><settlement>Lanzhou</settlement><postCode>730046</postCode><country key="CN" xml:lang="en">CHINA</country></address></affiliation></author><author><persName><forename type="first">Jie</forename><surname>Zhang</surname></persName><affiliation><orgName type="department">Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute</orgName><orgName type="institution">Chinese Academy of Agricultural Sciences</orgName><address><settlement>Lanzhou</settlement><postCode>730046</postCode><country key="CN" xml:lang="en">CHINA</country></address></affiliation></author><author><persName><forename type="first">De-hui</forename><surname>Sun</surname></persName><affiliation><orgName type="department">Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute</orgName><orgName type="institution">Chinese Academy of Agricultural Sciences</orgName><address><settlement>Lanzhou</settlement><postCode>730046</postCode><country key="CN" xml:lang="en">CHINA</country></address></affiliation></author><author><persName><forename type="first">Ji-le</forename><surname>Zhang</surname></persName><affiliation><orgName type="department">Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute</orgName><orgName type="institution">Chinese Academy of Agricultural Sciences</orgName><address><settlement>Lanzhou</settlement><postCode>730046</postCode><country key="CN" xml:lang="en">CHINA</country></address></affiliation></author><author><persName><forename type="first">Xue-peng</forename><surname>Cai</surname></persName><affiliation><orgName type="department">Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute</orgName><orgName type="institution">Chinese Academy of Agricultural Sciences</orgName><address><settlement>Lanzhou</settlement><postCode>730046</postCode><country key="CN" xml:lang="en">CHINA</country></address></affiliation></author><author><persName><forename type="first">Xiang-tao</forename><surname>Liu</surname></persName><affiliation><orgName type="department">Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute</orgName><orgName type="institution">Chinese Academy of Agricultural Sciences</orgName><address><settlement>Lanzhou</settlement><postCode>730046</postCode><country key="CN" xml:lang="en">CHINA</country></address></affiliation></author><author><persName><forename type="first">Yao-zhong</forename><surname>Ding</surname></persName><affiliation><orgName type="department">Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute</orgName><orgName type="institution">Chinese Academy of Agricultural Sciences</orgName><address><settlement>Lanzhou</settlement><postCode>730046</postCode><country key="CN" xml:lang="en">CHINA</country></address></affiliation></author><author><persName><forename type="first">Li-na</forename><surname>Ma</surname></persName><affiliation><orgName type="department">Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute</orgName><orgName type="institution">Chinese Academy of Agricultural Sciences</orgName><address><settlement>Lanzhou</settlement><postCode>730046</postCode><country key="CN" xml:lang="en">CHINA</country></address></affiliation></author><author><persName><forename type="first">Sheng-hai</forename><surname>Yang</surname></persName><affiliation><orgName type="department">Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute</orgName><orgName type="institution">Chinese Academy of Agricultural Sciences</orgName><address><settlement>Lanzhou</settlement><postCode>730046</postCode><country key="CN" xml:lang="en">CHINA</country></address></affiliation></author><author><persName><forename type="first">Lei</forename><surname>Jin</surname></persName><affiliation><orgName type="department">Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute</orgName><orgName type="institution">Chinese Academy of Agricultural Sciences</orgName><address><settlement>Lanzhou</settlement><postCode>730046</postCode><country key="CN" xml:lang="en">CHINA</country></address></affiliation></author><author role="corresp"><persName><forename type="first">Yongsheng</forename><surname>Liu</surname></persName><email>liuyongshengvip@sina.com.cn</email><affiliation><orgName type="department">Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute</orgName><orgName type="institution">Chinese Academy of Agricultural Sciences</orgName><address><settlement>Lanzhou</settlement><postCode>730046</postCode><country key="CN" xml:lang="en">CHINA</country></address></affiliation></author><idno 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<ref target="http://www.tei-c.org/">We use TEI</ref></desc></application></appInfo></encodingDesc><profileDesc><abstract xml:lang="en"><head>Abstract</head><p>Avian influenza and Newcastle disease are the highly contagious and most economically important diseases in poultry industry throughout the world. A multiplex reverse transcription polymerase chain reaction (mRT-PCR) assay was developed for the rapid and specific discrimination of H5 and H9 subtypes of avian influenza viruses (AIV) and Newcastle disease virus (NDV). Three sets of specific primers were applied in the assay based on the sequences of the hemagglutinin gene of H5-AIV, H9-AIV and fusion protein gene of NDV. 59 clinical samples including the throat washes, oral swabs, and cloacal scrapings were detected by mRT-PCR and single RT-PCR (sRT-PCR), respectively. The results indicated that the sensitivity and specifity of mRT-PCR were in accordance with sRT-PCR. The mRT-PCR developed in this study may therefore provide a new avenue to rapid detection of these important pathogens in one reaction.</p></abstract><textClass ana="subject"><keywords scheme="journal-subject"><list><item><term type="Primary">Medicine & Public Health</term></item><item><term type="Secondary">Zoology </term></item><item><term type="Secondary">Veterinary Medicine</term></item></list></keywords></textClass><textClass ana="abbreviation"><keywords scheme="abbreviations"><list></list></keywords></textClass><textClass ana="keyword"><keywords xml:lang="en"><term>Multiplex RT-PCR</term><term>Avian influenza</term><term>H5 subtype</term><term>H9 subtype</term><term>Newcastle disease</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc><revisionDesc><change when="2020-05-28" who="#istex" xml:id="pub2tei">formatting</change><change when="2008-04-02">Registration</change><change when="2007-10-12">Received</change><change 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NumberingStyle="Unnumbered"><JournalID>11259</JournalID><JournalPrintISSN>0165-7380</JournalPrintISSN><JournalElectronicISSN>1573-7446</JournalElectronicISSN><JournalTitle>Veterinary Research Communications</JournalTitle><JournalSubTitle>An International Journal Publishing Topical Reviews and Research Articles on all Aspects of the Veterinary Sciences</JournalSubTitle><JournalAbbreviatedTitle>Vet Res Commun</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Medicine & Public Health</JournalSubject><JournalSubject Type="Secondary">Zoology </JournalSubject><JournalSubject Type="Secondary">Veterinary Medicine</JournalSubject></JournalSubjectGroup></JournalInfo><Volume OutputMedium="All"><VolumeInfo TocLevels="0" VolumeType="Regular"><VolumeIDStart>32</VolumeIDStart><VolumeIDEnd>32</VolumeIDEnd><VolumeIssueCount>8</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular" OutputMedium="All"><IssueInfo IssueType="Regular" TocLevels="0"><IssueIDStart>6</IssueIDStart><IssueIDEnd>6</IssueIDEnd><IssueArticleCount>9</IssueArticleCount><IssueHistory><OnlineDate><Year>2008</Year><Month>7</Month><Day>12</Day></OnlineDate><PrintDate><Year>2008</Year><Month>7</Month><Day>12</Day></PrintDate><CoverDate><Year>2008</Year><Month>8</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Springer Science+Business Media B.V.</CopyrightHolderName><CopyrightYear>2008</CopyrightYear></IssueCopyright></IssueInfo><Article ID="s11259-008-9052-z" OutputMedium="All"><ArticleInfo ArticleType="OriginalPaper" ContainsESM="No" Language="En" NumberingStyle="Unnumbered" TocLevels="0"><ArticleID>9052</ArticleID><ArticleDOI>10.1007/s11259-008-9052-z</ArticleDOI><ArticleCitationID>491</ArticleCitationID><ArticleSequenceNumber>9</ArticleSequenceNumber><ArticleTitle Language="En">Rapid discrimination of H5 and H9 subtypes of avian influenza viruses and Newcastle disease virus by multiplex RT-PCR</ArticleTitle><ArticleCategory>Original Article</ArticleCategory><ArticleFirstPage>491</ArticleFirstPage><ArticleLastPage>498</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2008</Year><Month>4</Month><Day>2</Day></RegistrationDate><Received><Year>2007</Year><Month>10</Month><Day>12</Day></Received><Accepted><Year>2008</Year><Month>4</Month><Day>2</Day></Accepted><OnlineDate><Year>2008</Year><Month>5</Month><Day>15</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer Science+Business Media B.V.</CopyrightHolderName><CopyrightYear>2008</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Hao-tai</GivenName><FamilyName>Chen</FamilyName></AuthorName><Contact><Email>haotaichen@tom.com</Email></Contact></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Jie</GivenName><FamilyName>Zhang</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>De-hui</GivenName><FamilyName>Sun</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Ji-le</GivenName><FamilyName>Zhang</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Xue-peng</GivenName><FamilyName>Cai</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Xiang-tao</GivenName><FamilyName>Liu</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Yao-zhong</GivenName><FamilyName>Ding</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Li-na</GivenName><FamilyName>Ma</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Sheng-hai</GivenName><FamilyName>Yang</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Lei</GivenName><FamilyName>Jin</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Yongsheng</GivenName><FamilyName>Liu</FamilyName></AuthorName><Contact><Phone>+86-931-8342166</Phone><Fax>+86-931-8340977</Fax><Email>liuyongshengvip@sina.com.cn</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute</OrgDivision><OrgName>Chinese Academy of Agricultural Sciences</OrgName><OrgAddress><City>Lanzhou</City><Postcode>730046</Postcode><Country Code="CN">China</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para TextBreak="No">Avian influenza and Newcastle disease are the highly contagious and most economically important diseases in poultry industry throughout the world. A multiplex reverse transcription polymerase chain reaction (mRT-PCR) assay was developed for the rapid and specific discrimination of H5 and H9 subtypes of avian influenza viruses (AIV) and Newcastle disease virus (NDV). Three sets of specific primers were applied in the assay based on the sequences of the hemagglutinin gene of H5-AIV, H9-AIV and fusion protein gene of NDV. 59 clinical samples including the throat washes, oral swabs, and cloacal scrapings were detected by mRT-PCR and single RT-PCR (sRT-PCR), respectively. The results indicated that the sensitivity and specifity of mRT-PCR were in accordance with sRT-PCR. The mRT-PCR developed in this study may therefore provide a new avenue to rapid detection of these important pathogens in one reaction.</Para></Abstract><KeywordGroup Language="En"><Heading>Keywords</Heading><Keyword>Multiplex RT-PCR</Keyword><Keyword>Avian influenza</Keyword><Keyword>H5 subtype</Keyword><Keyword>H9 subtype</Keyword><Keyword>Newcastle disease</Keyword></KeywordGroup><AbbreviationGroup><AbbreviationGroupSection ID="AGS1"><Heading>Abbreviations</Heading><DefinitionList><DefinitionListEntry><Term>AI</Term><Description><Para TextBreak="No">avian influenza</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>AIV</Term><Description><Para TextBreak="No">avian influenza virus</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>F</Term><Description><Para TextBreak="No">fusion protein</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>HA</Term><Description><Para TextBreak="No">hemagglutinin</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>mRT-PCR</Term><Description><Para TextBreak="No">multiplex RT-PCR</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>ND</Term><Description><Para TextBreak="No">Newcastle disease</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>NDV</Term><Description><Para TextBreak="No">Newcastle disease virus</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>RT-PCR</Term><Description><Para TextBreak="No">reverse transcription polymerase chain reaction</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>sRT-PCR</Term><Description><Para TextBreak="No">single RT-PCR</Para></Description></DefinitionListEntry></DefinitionList></AbbreviationGroupSection></AbbreviationGroup><ArticleNote Type="Misc"><SimplePara>Hao-tai Chen and Jie Zhang contributed equally to this work.</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Rapid discrimination of H5 and H9 subtypes of avian influenza viruses and Newcastle disease virus by multiplex RT-PCR</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Rapid discrimination of H5 and H9 subtypes of avian influenza viruses and Newcastle disease virus by multiplex RT-PCR</title></titleInfo><name type="personal"><namePart type="given">Hao-tai</namePart><namePart type="family">Chen</namePart><affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</affiliation><affiliation>E-mail: haotaichen@tom.com</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jie</namePart><namePart type="family">Zhang</namePart><affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">De-hui</namePart><namePart type="family">Sun</namePart><affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ji-le</namePart><namePart type="family">Zhang</namePart><affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Xue-peng</namePart><namePart type="family">Cai</namePart><affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Xiang-tao</namePart><namePart type="family">Liu</namePart><affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yao-zhong</namePart><namePart type="family">Ding</namePart><affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Li-na</namePart><namePart type="family">Ma</namePart><affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sheng-hai</namePart><namePart type="family">Yang</namePart><affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Lei</namePart><namePart type="family">Jin</namePart><affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Yongsheng</namePart><namePart type="family">Liu</namePart><affiliation>Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China</affiliation><affiliation>E-mail: liuyongshengvip@sina.com.cn</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer Netherlands</publisher><place><placeTerm type="text">Dordrecht</placeTerm></place><dateCreated encoding="w3cdtf">2007-10-12</dateCreated><dateIssued encoding="w3cdtf">2008-08-01</dateIssued><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Avian influenza and Newcastle disease are the highly contagious and most economically important diseases in poultry industry throughout the world. A multiplex reverse transcription polymerase chain reaction (mRT-PCR) assay was developed for the rapid and specific discrimination of H5 and H9 subtypes of avian influenza viruses (AIV) and Newcastle disease virus (NDV). Three sets of specific primers were applied in the assay based on the sequences of the hemagglutinin gene of H5-AIV, H9-AIV and fusion protein gene of NDV. 59 clinical samples including the throat washes, oral swabs, and cloacal scrapings were detected by mRT-PCR and single RT-PCR (sRT-PCR), respectively. The results indicated that the sensitivity and specifity of mRT-PCR were in accordance with sRT-PCR. The mRT-PCR developed in this study may therefore provide a new avenue to rapid detection of these important pathogens in one reaction.</abstract><note>Original Article</note><subject lang="en"><genre>Keywords</genre><topic>Multiplex RT-PCR</topic><topic>Avian influenza</topic><topic>H5 subtype</topic><topic>H9 subtype</topic><topic>Newcastle disease</topic></subject><subject></subject><relatedItem type="host"><titleInfo><title>Veterinary Research Communications</title><subTitle>An International Journal Publishing Topical Reviews and Research Articles on all Aspects of the Veterinary Sciences</subTitle></titleInfo><titleInfo type="abbreviated"><title>Vet Res Commun</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2008</dateIssued><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><subject><genre>Medicine & Public Health</genre><topic>Zoology</topic><topic>Veterinary Medicine</topic></subject><identifier type="ISSN">0165-7380</identifier><identifier type="eISSN">1573-7446</identifier><identifier type="JournalID">11259</identifier><identifier type="IssueArticleCount">9</identifier><identifier type="VolumeIssueCount">8</identifier><part><date>2008</date><detail type="volume"><number>32</number><caption>vol.</caption></detail><detail type="issue"><number>6</number><caption>no.</caption></detail><extent unit="pages"><start>491</start><end>498</end></extent></part><recordInfo><recordOrigin>Springer Science+Business Media B.V., 2008</recordOrigin></recordInfo></relatedItem><identifier type="istex">0BB44734E28DE0D0E5CDF16BE2028ABB79AFF4CE</identifier><identifier type="ark">ark:/67375/VQC-1LJNJKKP-Q</identifier><identifier type="DOI">10.1007/s11259-008-9052-z</identifier><identifier type="ArticleID">9052</identifier><identifier type="ArticleID">s11259-008-9052-z</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer Science+Business Media B.V., 2008</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Converted from (version 1.2.14) to MODS version 3.6.</recordOrigin><recordCreationDate encoding="w3cdtf">2020-05-29</recordCreationDate></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-1LJNJKKP-Q/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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