Superior protection provided by a single dose of MF59-adjuvanted whole inactivated H5N1 influenza vaccine in type 1 diabetic mice
Identifieur interne : 000C94 ( Istex/Checkpoint ); précédent : 000C93; suivant : 000C95Superior protection provided by a single dose of MF59-adjuvanted whole inactivated H5N1 influenza vaccine in type 1 diabetic mice
Auteurs : Jian Wu [République populaire de Chine] ; Fuyan Wang [République populaire de Chine] ; Fang Fang [République populaire de Chine] ; Wenjie Zhang [République populaire de Chine] ; Haiyan Chang [République populaire de Chine] ; Liyun Zheng [République populaire de Chine] ; Ze Chen [République populaire de Chine, Niger]Source :
- Archives of Virology [ 0304-8608 ] ; 2011.
Abstract
Abstract: Vaccination is the preferred strategy for the prevention of influenza virus infection. Both H5N1 subunit and split vaccines have shown poor immunogenicity in clinical trials thus far. Therefore, it is urgent to develop more immunogenic and antigen-sparing H5N1 influenza vaccines as well as safe and effective adjuvants for humans, especially for immunocompromised people such as patients with diabetes mellitus. In this study, the protective effect of an MF59-adjuvanted inactivated whole-virion H5N1 vaccine was investigated in a type 1 diabetic mouse model. Mice (both healthy and diabetic) were immunized with a single dose of the inactivated vaccine, alone or adjuvanted with MF59 or Al(OH)3. After four weeks, mice were challenged with a lethal dose of H5N1 virus. Antibody responses, survival rates, lung virus titers and body weight changes were tested. The results showed that addition of MF59 or Al(OH)3 to the vaccine enhanced the antibody responses in both healthy mice and diabetic mice, but the MF59-adjuvanted groups showed higher antibody responses than the Al(OH)3-adjuvanted groups. The addition of MF59 or Al(OH)3 to the vaccine led to a conversion of the immune response from a Th1-biased response to an enhanced mixed Th1/Th2 profile. The MF59-adjuvanted inactivated whole-virion H5N1 vaccine provided superior protection in type 1 diabetic mice to either the vaccine alone or the vaccine adjuvanted with Al(OH)3.
Url:
DOI: 10.1007/s00705-010-0860-4
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
ISTEX:678A4C11B338AAAD11D12FE53BEF4769151F2F83Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Superior protection provided by a single dose of MF59-adjuvanted whole inactivated H5N1 influenza vaccine in type 1 diabetic mice</title><author><name sortKey="Wu, Jian" sort="Wu, Jian" uniqKey="Wu J" first="Jian" last="Wu">Jian Wu</name></author><author><name sortKey="Wang, Fuyan" sort="Wang, Fuyan" uniqKey="Wang F" first="Fuyan" last="Wang">Fuyan Wang</name></author><author><name sortKey="Fang, Fang" sort="Fang, Fang" uniqKey="Fang F" first="Fang" last="Fang">Fang Fang</name></author><author><name sortKey="Zhang, Wenjie" sort="Zhang, Wenjie" uniqKey="Zhang W" first="Wenjie" last="Zhang">Wenjie Zhang</name></author><author><name sortKey="Chang, Haiyan" sort="Chang, Haiyan" uniqKey="Chang H" first="Haiyan" last="Chang">Haiyan Chang</name></author><author><name sortKey="Zheng, Liyun" sort="Zheng, Liyun" uniqKey="Zheng L" first="Liyun" last="Zheng">Liyun Zheng</name></author><author><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:678A4C11B338AAAD11D12FE53BEF4769151F2F83</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1007/s00705-010-0860-4</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-FJCS526S-R/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000A69</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000A69</idno><idno type="wicri:Area/Istex/Curation">000A69</idno><idno type="wicri:Area/Istex/Checkpoint">000C94</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000C94</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Superior protection provided by a single dose of MF59-adjuvanted whole inactivated H5N1 influenza vaccine in type 1 diabetic mice</title><author><name sortKey="Wu, Jian" sort="Wu, Jian" uniqKey="Wu J" first="Jian" last="Wu">Jian Wu</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Sciences, Hunan Normal University, 410081, Changsha, Hunan</wicri:regionArea><wicri:noRegion>Hunan</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Fuyan" sort="Wang, Fuyan" uniqKey="Wang F" first="Fuyan" last="Wang">Fuyan Wang</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Sciences, Hunan Normal University, 410081, Changsha, Hunan</wicri:regionArea><wicri:noRegion>Hunan</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Immunology, Xiangya School of Medicine, Central South University, 410078, Changsha</wicri:regionArea><wicri:noRegion>Changsha</wicri:noRegion></affiliation></author><author><name sortKey="Fang, Fang" sort="Fang, Fang" uniqKey="Fang F" first="Fang" last="Fang">Fang Fang</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Sciences, Hunan Normal University, 410081, Changsha, Hunan</wicri:regionArea><wicri:noRegion>Hunan</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Wenjie" sort="Zhang, Wenjie" uniqKey="Zhang W" first="Wenjie" last="Zhang">Wenjie Zhang</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Xinhua Hospital Affiliated to Shanghai Jiaotong University of Medicine, 200092, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Chang, Haiyan" sort="Chang, Haiyan" uniqKey="Chang H" first="Haiyan" last="Chang">Haiyan Chang</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Sciences, Hunan Normal University, 410081, Changsha, Hunan</wicri:regionArea><wicri:noRegion>Hunan</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Liyun" sort="Zheng, Liyun" uniqKey="Zheng L" first="Liyun" last="Zheng">Liyun Zheng</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, Hubei</wicri:regionArea><wicri:noRegion>Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Sciences, Hunan Normal University, 410081, Changsha, Hunan</wicri:regionArea><wicri:noRegion>Hunan</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, Hubei</wicri:regionArea><wicri:noRegion>Hubei</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shanghai Institute of Biological Products, 200052, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Niger</country></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Archives of Virology</title><title level="j" type="abbrev">Arch Virol</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher ref="https://scientific-publisher.data.istex.fr/ark:/67375/H02-SWLMH5L1-1">Springer Vienna</publisher><pubPlace>Vienna</pubPlace><date type="published" when="2011">2011</date><biblScope unit="vol" from="156" to="156">156</biblScope><biblScope unit="issue" from="3" to="3">3</biblScope><biblScope unit="page" from="387">387</biblScope><biblScope unit="page" to="395">395</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Vaccination is the preferred strategy for the prevention of influenza virus infection. Both H5N1 subunit and split vaccines have shown poor immunogenicity in clinical trials thus far. Therefore, it is urgent to develop more immunogenic and antigen-sparing H5N1 influenza vaccines as well as safe and effective adjuvants for humans, especially for immunocompromised people such as patients with diabetes mellitus. In this study, the protective effect of an MF59-adjuvanted inactivated whole-virion H5N1 vaccine was investigated in a type 1 diabetic mouse model. Mice (both healthy and diabetic) were immunized with a single dose of the inactivated vaccine, alone or adjuvanted with MF59 or Al(OH)3. After four weeks, mice were challenged with a lethal dose of H5N1 virus. Antibody responses, survival rates, lung virus titers and body weight changes were tested. The results showed that addition of MF59 or Al(OH)3 to the vaccine enhanced the antibody responses in both healthy mice and diabetic mice, but the MF59-adjuvanted groups showed higher antibody responses than the Al(OH)3-adjuvanted groups. The addition of MF59 or Al(OH)3 to the vaccine led to a conversion of the immune response from a Th1-biased response to an enhanced mixed Th1/Th2 profile. The MF59-adjuvanted inactivated whole-virion H5N1 vaccine provided superior protection in type 1 diabetic mice to either the vaccine alone or the vaccine adjuvanted with Al(OH)3.</div></front></TEI><affiliations><list><country><li>Niger</li><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wu, Jian" sort="Wu, Jian" uniqKey="Wu J" first="Jian" last="Wu">Jian Wu</name></noRegion><name sortKey="Chang, Haiyan" sort="Chang, Haiyan" uniqKey="Chang H" first="Haiyan" last="Chang">Haiyan Chang</name><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><name sortKey="Fang, Fang" sort="Fang, Fang" uniqKey="Fang F" first="Fang" last="Fang">Fang Fang</name><name sortKey="Wang, Fuyan" sort="Wang, Fuyan" uniqKey="Wang F" first="Fuyan" last="Wang">Fuyan Wang</name><name sortKey="Wang, Fuyan" sort="Wang, Fuyan" uniqKey="Wang F" first="Fuyan" last="Wang">Fuyan Wang</name><name sortKey="Zhang, Wenjie" sort="Zhang, Wenjie" uniqKey="Zhang W" first="Wenjie" last="Zhang">Wenjie Zhang</name><name sortKey="Zheng, Liyun" sort="Zheng, Liyun" uniqKey="Zheng L" first="Liyun" last="Zheng">Liyun Zheng</name></country><country name="Niger"><noRegion><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/PandemieGrippaleV1/Data/Istex/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C94 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Checkpoint/biblio.hfd -nk 000C94 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= PandemieGrippaleV1
|flux= Istex
|étape= Checkpoint
|type= RBID
|clé= ISTEX:678A4C11B338AAAD11D12FE53BEF4769151F2F83
|texte= Superior protection provided by a single dose of MF59-adjuvanted whole inactivated H5N1 influenza vaccine in type 1 diabetic mice
}}
| This area was generated with Dilib version V0.6.34. |  |