A continuous peptide epitope reacting with pandemic influenza AH1N1 predicted by bioinformatic approaches
Identifieur interne : 000194 ( Istex/Checkpoint ); précédent : 000193; suivant : 000195A continuous peptide epitope reacting with pandemic influenza AH1N1 predicted by bioinformatic approaches
Auteurs : Jonathan P. Carrillo-Vazquez [Mexique] ; José Correa-Basurto [Mexique] ; Jazmin García-Machorro [Mexique] ; Rafael Campos-Rodríguez [Mexique] ; Violaine Moreau [France] ; Jorge L. Rosas-Trigueros [Mexique] ; Cesar A. Reyes-L Pez [Mexique] ; Marlon Rojas-L Pez [Mexique] ; Absalom Zamorano-Carrillo [Mexique]Source :
- Journal of Molecular Recognition [ 0952-3499 ] ; 2015-09.
Abstract
Computational identification of potential epitopes with an immunogenic capacity challenges immunological research. Several methods show considerable success, and together with experimental studies, the efficiency of the algorithms to identify potential peptides with biological activity has improved. Herein, an epitope was designed by combining bioinformatics, docking, and molecular dynamics simulations. The hemagglutinin protein of the H1N1 influenza pandemic strain served as a template, owing to the interest of obtaining a scheme of immunization. Afterward, we performed enzyme‐linked immunosorbent assay (ELISA) using the epitope to analyze if any antibodies in human sera before and after the influenza outbreak in 2009 recognize this peptide. Also, a plaque reduction neutralization test induced by virus‐neutralizing antibodies and the IgG determination showed the biological activity of this computationally designed peptide. The results of the ELISAs demonstrated that the serum of both prepandemic and pandemic recognized the epitope. Moreover, the plaque reduction neutralization test evidenced the capacity of the designed peptide to neutralize influenza virus in Madin‐Darby canine cells. Copyright © 2015 John Wiley & Sons, Ltd.
Computational identification of epitopes with immunogenic capacity shows considerable success, we design a peptide using bioinformatics, docking and molecular dynamics simulation to obtain a H1N1 epitope using the Hemagglutinin protein. Afterward we performed ELISA assays with human sera and a plaque reduction neutralization test demonstrating the capacity of this designed peptide against the Influenza Virus in Madin Darby Kanine Cells.
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DOI: 10.1002/jmr.2470
Affiliations:
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Several methods show considerable success, and together with experimental studies, the efficiency of the algorithms to identify potential peptides with biological activity has improved. Herein, an epitope was designed by combining bioinformatics, docking, and molecular dynamics simulations. The hemagglutinin protein of the H1N1 influenza pandemic strain served as a template, owing to the interest of obtaining a scheme of immunization. Afterward, we performed enzyme‐linked immunosorbent assay (ELISA) using the epitope to analyze if any antibodies in human sera before and after the influenza outbreak in 2009 recognize this peptide. Also, a plaque reduction neutralization test induced by virus‐neutralizing antibodies and the IgG determination showed the biological activity of this computationally designed peptide. The results of the ELISAs demonstrated that the serum of both prepandemic and pandemic recognized the epitope. Moreover, the plaque reduction neutralization test evidenced the capacity of the designed peptide to neutralize influenza virus in Madin‐Darby canine cells. Copyright © 2015 John Wiley & Sons, Ltd.</div><div type="abstract">Computational identification of epitopes with immunogenic capacity shows considerable success, we design a peptide using bioinformatics, docking and molecular dynamics simulation to obtain a H1N1 epitope using the Hemagglutinin protein. Afterward we performed ELISA assays with human sera and a plaque reduction neutralization test demonstrating the capacity of this designed peptide against the Influenza Virus in Madin Darby Kanine Cells.</div></front></TEI><affiliations><list><country><li>France</li><li>Mexique</li></country><region><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li></region><settlement><li>Montpellier</li></settlement></list><tree><country name="Mexique"><noRegion><name sortKey="Carrillo Azquez, Jonathan P" sort="Carrillo Azquez, Jonathan P" uniqKey="Carrillo Azquez J" first="Jonathan P." last="Carrillo-Vazquez">Jonathan P. Carrillo-Vazquez</name></noRegion><name sortKey="Campos Odriguez, Rafael" sort="Campos Odriguez, Rafael" uniqKey="Campos Odriguez R" first="Rafael" last="Campos-Rodríguez">Rafael Campos-Rodríguez</name><name sortKey="Correa Asurto, Jose" sort="Correa Asurto, Jose" uniqKey="Correa Asurto J" first="José" last="Correa-Basurto">José Correa-Basurto</name><name sortKey="Garcia Achorro, Jazmin" sort="Garcia Achorro, Jazmin" uniqKey="Garcia Achorro J" first="Jazmin" last="García-Machorro">Jazmin García-Machorro</name><name sortKey="Reyes Pez, Cesar A" sort="Reyes Pez, Cesar A" uniqKey="Reyes Pez C" first="Cesar A." last="Reyes-L Pez">Cesar A. Reyes-L Pez</name><name sortKey="Rojas Pez, Marlon" sort="Rojas Pez, Marlon" uniqKey="Rojas Pez M" first="Marlon" last="Rojas-L Pez">Marlon Rojas-L Pez</name><name sortKey="Rosas Rigueros, Jorge L" sort="Rosas Rigueros, Jorge L" uniqKey="Rosas Rigueros J" first="Jorge L." last="Rosas-Trigueros">Jorge L. Rosas-Trigueros</name><name sortKey="Zamorano Arrillo, Absalom" sort="Zamorano Arrillo, Absalom" uniqKey="Zamorano Arrillo A" first="Absalom" last="Zamorano-Carrillo">Absalom Zamorano-Carrillo</name></country><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Moreau, Violaine" sort="Moreau, Violaine" uniqKey="Moreau V" first="Violaine" last="Moreau">Violaine Moreau</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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