Neutralizing immune responses induced by oligomeric H5N1-hemagglutinins from plants
Identifieur interne : 000419 ( Hal/Curation ); précédent : 000418; suivant : 000420Neutralizing immune responses induced by oligomeric H5N1-hemagglutinins from plants
Auteurs : Hoang Trong Phan ; Thuong Thi Ho ; Ha Hoang Chu ; Trang Huyen Vu ; Ulrike Gresch ; Udo ConradSource :
- Veterinary Research [ 0928-4249 ] ; 2017-12.
Abstract
AbstractPlant-based transient expression is an alternative platform to produce hemagglutinin-based subunit vaccines. This production system provides not only fast and effective response in the context of a pandemic but also enables the supply of big volume vaccines at low cost. Crude plant extracts containing influenza hemagglutinin are considered to use as vaccine sources because of avoidance of related purification steps resulting in low cost production allowing veterinary applications. Highly immunogenic influenza hemagglutinins are urgently required to meet these pre-conditions. Here, we present a new and innovative way to generate functional H5 oligomers from avian flu hemagglutinin in planta by the specific interaction of S·Tag and S·Protein. A S·Tag was fused to H5 trimers and this construct was transiently co-expressed in planta with S·Protein-TPs which was multimerized by disulfide bonds via cysteine residues in tailpiece sequences (TP) of IgM antibody. Multimerized S·Protein-TPs serve as bridges/molecular docks to combine S·Tag-fused hemagglutinin trimers to form very large hemagglutinin H5 oligomers. H5 oligomers in the plant crude extract were highly active in hemagglutination resulting in high titers. Immunization of mice with two doses of plant crude extracts containing H5 oligomers after storage for 1 week at 4 °C caused strong immune responses and induced neutralizing specific humoral immune responses in mice. These results allow for the development of cheap influenza vaccines for veterinary application in future.
Url:
DOI: 10.1186/s13567-017-0458-x
Links toward previous steps (curation, corpus...)
- to stream Hal, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000419
Links to Exploration step
Hal:hal-01591129Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Neutralizing immune responses induced by oligomeric H5N1-hemagglutinins from plants</title>
<author><name sortKey="Phan, Hoang Trong" sort="Phan, Hoang Trong" uniqKey="Phan H" first="Hoang Trong" last="Phan">Hoang Trong Phan</name>
</author>
<author><name sortKey="Ho, Thuong Thi" sort="Ho, Thuong Thi" uniqKey="Ho T" first="Thuong Thi" last="Ho">Thuong Thi Ho</name>
</author>
<author><name sortKey="Chu, Ha Hoang" sort="Chu, Ha Hoang" uniqKey="Chu H" first="Ha Hoang" last="Chu">Ha Hoang Chu</name>
</author>
<author><name sortKey="Vu, Trang Huyen" sort="Vu, Trang Huyen" uniqKey="Vu T" first="Trang Huyen" last="Vu">Trang Huyen Vu</name>
</author>
<author><name sortKey="Gresch, Ulrike" sort="Gresch, Ulrike" uniqKey="Gresch U" first="Ulrike" last="Gresch">Ulrike Gresch</name>
</author>
<author><name sortKey="Conrad, Udo" sort="Conrad, Udo" uniqKey="Conrad U" first="Udo" last="Conrad">Udo Conrad</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">HAL</idno>
<idno type="RBID">Hal:hal-01591129</idno>
<idno type="halId">hal-01591129</idno>
<idno type="halUri">https://hal.archives-ouvertes.fr/hal-01591129</idno>
<idno type="url">https://hal.archives-ouvertes.fr/hal-01591129</idno>
<idno type="doi">10.1186/s13567-017-0458-x</idno>
<date when="2017-12">2017-12</date>
<idno type="wicri:Area/Hal/Corpus">000419</idno>
<idno type="wicri:Area/Hal/Curation">000419</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Neutralizing immune responses induced by oligomeric H5N1-hemagglutinins from plants</title>
<author><name sortKey="Phan, Hoang Trong" sort="Phan, Hoang Trong" uniqKey="Phan H" first="Hoang Trong" last="Phan">Hoang Trong Phan</name>
</author>
<author><name sortKey="Ho, Thuong Thi" sort="Ho, Thuong Thi" uniqKey="Ho T" first="Thuong Thi" last="Ho">Thuong Thi Ho</name>
</author>
<author><name sortKey="Chu, Ha Hoang" sort="Chu, Ha Hoang" uniqKey="Chu H" first="Ha Hoang" last="Chu">Ha Hoang Chu</name>
</author>
<author><name sortKey="Vu, Trang Huyen" sort="Vu, Trang Huyen" uniqKey="Vu T" first="Trang Huyen" last="Vu">Trang Huyen Vu</name>
</author>
<author><name sortKey="Gresch, Ulrike" sort="Gresch, Ulrike" uniqKey="Gresch U" first="Ulrike" last="Gresch">Ulrike Gresch</name>
</author>
<author><name sortKey="Conrad, Udo" sort="Conrad, Udo" uniqKey="Conrad U" first="Udo" last="Conrad">Udo Conrad</name>
</author>
</analytic>
<idno type="DOI">10.1186/s13567-017-0458-x</idno>
<series><title level="j">Veterinary Research</title>
<idno type="ISSN">0928-4249</idno>
<imprint><date type="datePub">2017-12</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"> <p>AbstractPlant-based transient expression is an alternative platform to produce hemagglutinin-based subunit vaccines. This production system provides not only fast and effective response in the context of a pandemic but also enables the supply of big volume vaccines at low cost. Crude plant extracts containing influenza hemagglutinin are considered to use as vaccine sources because of avoidance of related purification steps resulting in low cost production allowing veterinary applications. Highly immunogenic influenza hemagglutinins are urgently required to meet these pre-conditions. Here, we present a new and innovative way to generate functional H5 oligomers from avian flu hemagglutinin in planta by the specific interaction of S·Tag and S·Protein. A S·Tag was fused to H5 trimers and this construct was transiently co-expressed in planta with S·Protein-TPs which was multimerized by disulfide bonds via cysteine residues in tailpiece sequences (TP) of IgM antibody. Multimerized S·Protein-TPs serve as bridges/molecular docks to combine S·Tag-fused hemagglutinin trimers to form very large hemagglutinin H5 oligomers. H5 oligomers in the plant crude extract were highly active in hemagglutination resulting in high titers. Immunization of mice with two doses of plant crude extracts containing H5 oligomers after storage for 1 week at 4 °C caused strong immune responses and induced neutralizing specific humoral immune responses in mice. These results allow for the development of cheap influenza vaccines for veterinary application in future.</p>
</div>
</front>
</TEI>
<hal api="V3"> <titleStmt> <title xml:lang="en">Neutralizing immune responses induced by oligomeric H5N1-hemagglutinins from plants</title>
<author role="aut"> <persName> <forename type="first">Hoang Trong</forename>
<surname>Phan</surname>
</persName>
<email type="md5">1b67abd0026c2355efa65353ab10af65</email>
<email type="domain">ipk-gatersleben.de</email>
<idno type="halauthorid">1619225</idno>
</author>
<author role="aut"> <persName> <forename type="first">Thuong Thi</forename>
<surname>Ho</surname>
</persName>
<email type="md5">a5e3c7c343a20194d934b03dc8358409</email>
<email type="domain">gmail.com</email>
<idno type="halauthorid">1619226</idno>
</author>
<author role="aut"> <persName> <forename type="first">Ha Hoang</forename>
<surname>Chu</surname>
</persName>
<email type="md5">c7f01929094cc9331179a1722f3e19e5</email>
<email type="domain">ibt.ac.vn</email>
<idno type="halauthorid">1619227</idno>
</author>
<author role="aut"> <persName> <forename type="first">Trang Huyen</forename>
<surname>Vu</surname>
</persName>
<email type="md5">e5c5c1e568927523338b8c383c887a24</email>
<email type="domain">gmail.com</email>
<idno type="halauthorid">1619228</idno>
</author>
<author role="aut"> <persName> <forename type="first">Ulrike</forename>
<surname>Gresch</surname>
</persName>
<email type="md5">68523c262e113bd871870459bde273b1</email>
<email type="domain">ipk-gatersleben.de</email>
<idno type="halauthorid">1619229</idno>
</author>
<author role="crp"> <persName> <forename type="first">Udo</forename>
<surname>Conrad</surname>
</persName>
<email type="md5">011463265a6b9de8c1966b61b5eb94bf</email>
<email type="domain">ipk-gatersleben.de</email>
<idno type="halauthorid">1313295</idno>
</author>
<editor role="depositor"> <persName> <forename>BMC</forename>
<surname>BMC</surname>
</persName>
<email type="md5">750df22138c3a8cb85044e5ee79ee867</email>
<email type="domain">biomedcentral.com</email>
</editor>
</titleStmt>
<editionStmt> <edition n="v1" type="current"> <date type="whenSubmitted">2017-09-20 20:03:04</date>
<date type="whenModified">2017-09-23 01:05:59</date>
<date type="whenReleased">2017-09-21 17:49:19</date>
<date type="whenProduced">2017-12</date>
<date type="whenEndEmbargoed">2017-09-20</date>
<ref type="file" target="https://hal.archives-ouvertes.fr/hal-01591129/document"> <date notBefore="2017-09-20"></date>
</ref>
<ref type="file" subtype="publisherPaid" n="1" target="https://hal.archives-ouvertes.fr/hal-01591129/file/13567_2017_Article_458.pdf"> <date notBefore="2017-09-20"></date>
</ref>
<ref type="externalLink" target="https://veterinaryresearch.biomedcentral.com/track/pdf/10.1186/s13567-017-0458-x"></ref>
</edition>
<respStmt> <resp>contributor</resp>
<name key="326461"> <persName> <forename>BMC</forename>
<surname>BMC</surname>
</persName>
<email type="md5">750df22138c3a8cb85044e5ee79ee867</email>
<email type="domain">biomedcentral.com</email>
</name>
</respStmt>
</editionStmt>
<publicationStmt> <distributor>CCSD</distributor>
<idno type="halId">hal-01591129</idno>
<idno type="halUri">https://hal.archives-ouvertes.fr/hal-01591129</idno>
<idno type="halBibtex">phan:hal-01591129</idno>
<idno type="halRefHtml">Veterinary Research, BioMed Central, 2017, 48 (1), pp.53. ⟨10.1186/s13567-017-0458-x⟩</idno>
<idno type="halRef">Veterinary Research, BioMed Central, 2017, 48 (1), pp.53. ⟨10.1186/s13567-017-0458-x⟩</idno>
</publicationStmt>
<seriesStmt> <idno type="stamp" n="ARINRA-VETR" corresp="ARINRA">Veterinary research</idno>
<idno type="stamp" n="ARINRA">Archives des revues INRA</idno>
</seriesStmt>
<notesStmt> <note type="audience" n="2">International</note>
<note type="popular" n="0">No</note>
<note type="peer" n="1">Yes</note>
</notesStmt>
<sourceDesc> <biblStruct> <analytic> <title xml:lang="en">Neutralizing immune responses induced by oligomeric H5N1-hemagglutinins from plants</title>
<author role="aut"> <persName> <forename type="first">Hoang Trong</forename>
<surname>Phan</surname>
</persName>
<email type="md5">1b67abd0026c2355efa65353ab10af65</email>
<email type="domain">ipk-gatersleben.de</email>
<idno type="halauthorid">1619225</idno>
</author>
<author role="aut"> <persName> <forename type="first">Thuong Thi</forename>
<surname>Ho</surname>
</persName>
<email type="md5">a5e3c7c343a20194d934b03dc8358409</email>
<email type="domain">gmail.com</email>
<idno type="halauthorid">1619226</idno>
</author>
<author role="aut"> <persName> <forename type="first">Ha Hoang</forename>
<surname>Chu</surname>
</persName>
<email type="md5">c7f01929094cc9331179a1722f3e19e5</email>
<email type="domain">ibt.ac.vn</email>
<idno type="halauthorid">1619227</idno>
</author>
<author role="aut"> <persName> <forename type="first">Trang Huyen</forename>
<surname>Vu</surname>
</persName>
<email type="md5">e5c5c1e568927523338b8c383c887a24</email>
<email type="domain">gmail.com</email>
<idno type="halauthorid">1619228</idno>
</author>
<author role="aut"> <persName> <forename type="first">Ulrike</forename>
<surname>Gresch</surname>
</persName>
<email type="md5">68523c262e113bd871870459bde273b1</email>
<email type="domain">ipk-gatersleben.de</email>
<idno type="halauthorid">1619229</idno>
</author>
<author role="crp"> <persName> <forename type="first">Udo</forename>
<surname>Conrad</surname>
</persName>
<email type="md5">011463265a6b9de8c1966b61b5eb94bf</email>
<email type="domain">ipk-gatersleben.de</email>
<idno type="halauthorid">1313295</idno>
</author>
</analytic>
<monogr> <idno type="halJournalId" status="VALID">19840</idno>
<idno type="issn">0928-4249</idno>
<idno type="eissn">1297-9716</idno>
<title level="j">Veterinary Research</title>
<imprint> <publisher>BioMed Central</publisher>
<biblScope unit="volume">48</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="pp">53</biblScope>
<date type="datePub">2017-12</date>
<date type="dateEpub">2017</date>
</imprint>
</monogr>
<idno type="doi">10.1186/s13567-017-0458-x</idno>
</biblStruct>
</sourceDesc>
<profileDesc> <langUsage> <language ident="en">English</language>
</langUsage>
<textClass> <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
<classCode scheme="halTypology" n="ART">Journal articles</classCode>
</textClass>
<abstract xml:lang="en"> <p>AbstractPlant-based transient expression is an alternative platform to produce hemagglutinin-based subunit vaccines. This production system provides not only fast and effective response in the context of a pandemic but also enables the supply of big volume vaccines at low cost. Crude plant extracts containing influenza hemagglutinin are considered to use as vaccine sources because of avoidance of related purification steps resulting in low cost production allowing veterinary applications. Highly immunogenic influenza hemagglutinins are urgently required to meet these pre-conditions. Here, we present a new and innovative way to generate functional H5 oligomers from avian flu hemagglutinin in planta by the specific interaction of S·Tag and S·Protein. A S·Tag was fused to H5 trimers and this construct was transiently co-expressed in planta with S·Protein-TPs which was multimerized by disulfide bonds via cysteine residues in tailpiece sequences (TP) of IgM antibody. Multimerized S·Protein-TPs serve as bridges/molecular docks to combine S·Tag-fused hemagglutinin trimers to form very large hemagglutinin H5 oligomers. H5 oligomers in the plant crude extract were highly active in hemagglutination resulting in high titers. Immunization of mice with two doses of plant crude extracts containing H5 oligomers after storage for 1 week at 4 °C caused strong immune responses and induced neutralizing specific humoral immune responses in mice. These results allow for the development of cheap influenza vaccines for veterinary application in future.</p>
</abstract>
</profileDesc>
</hal>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/PandemieGrippaleV1/Data/Hal/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000419 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Hal/Curation/biblio.hfd -nk 000419 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= PandemieGrippaleV1 |flux= Hal |étape= Curation |type= RBID |clé= Hal:hal-01591129 |texte= Neutralizing immune responses induced by oligomeric H5N1-hemagglutinins from plants }}
![]() | This area was generated with Dilib version V0.6.34. | ![]() |