PandemieGrippaleV1, Hal, Corpus, bibRecord, 000675

Ultrastructural Characterization of Zika Virus Replication Factories.

Identifieur interne : 000675 ( Hal/Corpus ); précédent : 000674; suivant : 000676

Ultrastructural Characterization of Zika Virus Replication Factories.

Auteurs : Mirko Cortese ; Sarah Goellner ; Eliana Gisela Acosta ; Christopher John Neufeldt ; Olga Oleksiuk ; Marko Lampe ; Uta Haselmann ; Charlotta Funaya ; Nicole Schieber ; Paolo Ronchi ; Martin Schorb ; Priit Pruunsild ; Yannick Schwab ; Laurent Chatel-Chaix ; Alessia Ruggieri ; Ralf Bartenschlager

Source :

Cell Reports [ 2211-1247 ] ; 2017-02-28.

RBID : Hal:pasteur-01535196

English descriptors

mix :
- Zika virus, electron microscopy, electron tomography, flavivirus, human neural progenitor cells, intermediate filaments, live-cell imaging, microtubules, replication factories, replication organelles.

Abstract

A global concern has emerged with the pandemic spread of Zika virus (ZIKV) infections that can cause severe neurological symptoms in adults and newborns. ZIKV is a positive-strand RNA virus replicating in virus-induced membranous replication factories (RFs). Here we used various imaging techniques to investigate the ultrastructural details of ZIKV RFs and their relationship with host cell organelles. Analyses of human hepatic cells and neural progenitor cells infected with ZIKV revealed endoplasmic reticulum (ER) membrane invaginations containing pore-like openings toward the cytosol, reminiscent to RFs in Dengue virus-infected cells. Both the MR766 African strain and the H/PF/2013 Asian strain, the latter linked to neurological diseases, induce RFs of similar architecture. Importantly, ZIKV infection causes a drastic reorganization of microtubules and intermediate filaments forming cage-like structures surrounding the viral RF. Consistently, ZIKV replication is suppressed by cytoskeleton-targeting drugs. Thus, ZIKV RFs are tightly linked to rearrangements of the host cell cytoskeleton.

Url:

https://hal-riip.archives-ouvertes.fr/pasteur-01535196

DOI: 10.1016/j.celrep.2017.02.014

Links to Exploration step

Hal:pasteur-01535196

Le document en format XML

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<author><name sortKey="Schieber, Nicole" sort="Schieber, Nicole" uniqKey="Schieber N" first="Nicole" last="Schieber">Nicole Schieber</name>
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<author><name sortKey="Ronchi, Paolo" sort="Ronchi, Paolo" uniqKey="Ronchi P" first="Paolo" last="Ronchi">Paolo Ronchi</name>
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<author><name sortKey="Schorb, Martin" sort="Schorb, Martin" uniqKey="Schorb M" first="Martin" last="Schorb">Martin Schorb</name>
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<author><name sortKey="Pruunsild, Priit" sort="Pruunsild, Priit" uniqKey="Pruunsild P" first="Priit" last="Pruunsild">Priit Pruunsild</name>
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<author><name sortKey="Schwab, Yannick" sort="Schwab, Yannick" uniqKey="Schwab Y" first="Yannick" last="Schwab">Yannick Schwab</name>
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<author><name sortKey="Chatel Chaix, Laurent" sort="Chatel Chaix, Laurent" uniqKey="Chatel Chaix L" first="Laurent" last="Chatel-Chaix">Laurent Chatel-Chaix</name>
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<author><name sortKey="Ruggieri, Alessia" sort="Ruggieri, Alessia" uniqKey="Ruggieri A" first="Alessia" last="Ruggieri">Alessia Ruggieri</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-500956" status="INCOMING">          <orgName>Department of Infectious Diseases, Molecular Virology</orgName>
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<author><name sortKey="Bartenschlager, Ralf" sort="Bartenschlager, Ralf" uniqKey="Bartenschlager R" first="Ralf" last="Bartenschlager">Ralf Bartenschlager</name>
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<idno type="DOI">10.1016/j.celrep.2017.02.014</idno>
<series><title level="j">Cell Reports</title>
<idno type="ISSN">2211-1247</idno>
<imprint><date type="datePub">2017-02-28</date>
</imprint>
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<profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>Zika virus</term>
<term>electron microscopy</term>
<term>electron tomography</term>
<term>flavivirus</term>
<term>human neural progenitor cells</term>
<term>intermediate filaments</term>
<term>live-cell imaging</term>
<term>microtubules</term>
<term>replication factories</term>
<term>replication organelles</term>
</keywords>
</textClass>
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</teiHeader>
<front><div type="abstract" xml:lang="en">              <p>A global concern has emerged with the pandemic spread of Zika virus (ZIKV) infections that can cause severe neurological symptoms in adults and newborns. ZIKV is a positive-strand RNA virus replicating in virus-induced membranous replication factories (RFs). Here we used various imaging techniques to investigate the ultrastructural details of ZIKV RFs and their relationship with host cell organelles. Analyses of human hepatic cells and neural progenitor cells infected with ZIKV revealed endoplasmic reticulum (ER) membrane invaginations containing pore-like openings toward the cytosol, reminiscent to RFs in Dengue virus-infected cells. Both the MR766 African strain and the H/PF/2013 Asian strain, the latter linked to neurological diseases, induce RFs of similar architecture. Importantly, ZIKV infection causes a drastic reorganization of microtubules and intermediate filaments forming cage-like structures surrounding the viral RF. Consistently, ZIKV replication is suppressed by cytoskeleton-targeting drugs. Thus, ZIKV RFs are tightly linked to rearrangements of the host cell cytoskeleton.</p>
            </div>
</front>
</TEI>
<hal api="V3">          <titleStmt>            <title xml:lang="eng">Ultrastructural Characterization of Zika Virus Replication Factories.</title>
            <author role="aut">              <persName>                <forename type="first">Mirko</forename>
                <surname>Cortese</surname>
              </persName>
              <idno type="halauthorid">1555039</idno>
              <affiliation ref="#struct-500956"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Sarah</forename>
                <surname>Goellner</surname>
              </persName>
              <idno type="halauthorid">1555040</idno>
              <affiliation ref="#struct-500956"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Eliana Gisela</forename>
                <surname>Acosta</surname>
              </persName>
              <idno type="halauthorid">1555041</idno>
              <affiliation ref="#struct-500956"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Christopher John</forename>
                <surname>Neufeldt</surname>
              </persName>
              <idno type="halauthorid">1555042</idno>
              <affiliation ref="#struct-500956"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Olga</forename>
                <surname>Oleksiuk</surname>
              </persName>
              <idno type="halauthorid">1555043</idno>
              <affiliation ref="#struct-500956"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Marko</forename>
                <surname>Lampe</surname>
              </persName>
              <idno type="halauthorid">1555044</idno>
              <affiliation ref="#struct-30303"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Uta</forename>
                <surname>Haselmann</surname>
              </persName>
              <idno type="halauthorid">1555045</idno>
              <affiliation ref="#struct-500956"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Charlotta</forename>
                <surname>Funaya</surname>
              </persName>
              <idno type="halauthorid">1555046</idno>
              <affiliation ref="#struct-179987"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Nicole</forename>
                <surname>Schieber</surname>
              </persName>
              <idno type="halauthorid">1555047</idno>
              <affiliation ref="#struct-134947"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Paolo</forename>
                <surname>Ronchi</surname>
              </persName>
              <idno type="halauthorid">1555048</idno>
              <affiliation ref="#struct-30303"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Martin</forename>
                <surname>Schorb</surname>
              </persName>
              <idno type="halauthorid">1555049</idno>
              <affiliation ref="#struct-30303"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Priit</forename>
                <surname>Pruunsild</surname>
              </persName>
              <idno type="halauthorid">1555050</idno>
              <affiliation ref="#struct-306189"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Yannick</forename>
                <surname>Schwab</surname>
              </persName>
              <idno type="halauthorid">176745</idno>
              <affiliation ref="#struct-30303"></affiliation>
              <affiliation ref="#struct-134947"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Laurent</forename>
                <surname>Chatel-Chaix</surname>
              </persName>
              <idno type="halauthorid">1555051</idno>
              <affiliation ref="#struct-54664"></affiliation>
              <affiliation ref="#struct-500956"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">Alessia</forename>
                <surname>Ruggieri</surname>
              </persName>
              <idno type="halauthorid">1555052</idno>
              <affiliation ref="#struct-500956"></affiliation>
            </author>
            <author role="crp">              <persName>                <forename type="first">Ralf</forename>
                <surname>Bartenschlager</surname>
              </persName>
              <email type="md5">9daba53b67ba715d99e7e84334eafe41</email>
              <email type="domain">med.uni-heidelberg.de</email>
              <idno type="halauthorid">1581352</idno>
              <affiliation ref="#struct-500956"></affiliation>
              <affiliation ref="#struct-500957"></affiliation>
            </author>
            <editor role="depositor">              <persName>                <forename>Michel</forename>
                <surname>Courcelles</surname>
              </persName>
              <email type="md5">235ee71abfb5f3fb19e1b204192e01ee</email>
              <email type="domain">iaf.inrs.ca</email>
            </editor>
            <funder>This work was supported by grants from the Deutsche Forschungsgemeinschaft (SFB 1129 to R.B and A.R). L.C.-C. holds a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada (RGPIN-2016-05584). P.P. holds a fellowship by the Excellence Cluster Cell Networks at Heidelberg University. C.J.N is funded by a European Molecular Biology Organization (EMBO) Long-Term Fellowship (ALTF 466-2016) </funder>
          </titleStmt>
          <editionStmt>            <edition n="v1" type="current">              <date type="whenSubmitted">2017-06-08 20:19:11</date>
              <date type="whenModified">2020-05-18 11:00:35</date>
              <date type="whenReleased">2017-07-10 11:42:09</date>
              <date type="whenProduced">2017-02-28</date>
              <date type="whenEndEmbargoed">2017-07-10</date>
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            <respStmt>              <resp>contributor</resp>
              <name key="157387">                <persName>                  <forename>Michel</forename>
                  <surname>Courcelles</surname>
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                <email type="md5">235ee71abfb5f3fb19e1b204192e01ee</email>
                <email type="domain">iaf.inrs.ca</email>
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            <idno type="halRefHtml">Cell Reports, Elsevier Inc, 2017, 18 (9), pp.2113-2123. ⟨10.1016/j.celrep.2017.02.014⟩</idno>
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          <seriesStmt>            <idno type="stamp" n="RIIP">Institut Pasteur RIIP (Réseau International)</idno>
            <idno type="stamp" n="INRS-IAF" corresp="RIIP">Institut Armand Frappier</idno>
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          <notesStmt>            <note type="audience" n="2">International</note>
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          <sourceDesc>            <biblStruct>              <analytic>                <title xml:lang="eng">Ultrastructural Characterization of Zika Virus Replication Factories.</title>
                <author role="aut">                  <persName>                    <forename type="first">Mirko</forename>
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                  </persName>
                  <idno type="halauthorid">1555039</idno>
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                </author>
                <author role="aut">                  <persName>                    <forename type="first">Sarah</forename>
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                  <idno type="halauthorid">1555040</idno>
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                </author>
                <author role="aut">                  <persName>                    <forename type="first">Eliana Gisela</forename>
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                </author>
                <author role="aut">                  <persName>                    <forename type="first">Christopher John</forename>
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                <author role="aut">                  <persName>                    <forename type="first">Olga</forename>
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                  <idno type="halauthorid">1555043</idno>
                  <affiliation ref="#struct-500956"></affiliation>
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                <author role="aut">                  <persName>                    <forename type="first">Marko</forename>
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                  <idno type="halauthorid">1555044</idno>
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                </author>
                <author role="aut">                  <persName>                    <forename type="first">Uta</forename>
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                  <idno type="halauthorid">1555045</idno>
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                </author>
                <author role="aut">                  <persName>                    <forename type="first">Charlotta</forename>
                    <surname>Funaya</surname>
                  </persName>
                  <idno type="halauthorid">1555046</idno>
                  <affiliation ref="#struct-179987"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Nicole</forename>
                    <surname>Schieber</surname>
                  </persName>
                  <idno type="halauthorid">1555047</idno>
                  <affiliation ref="#struct-134947"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Paolo</forename>
                    <surname>Ronchi</surname>
                  </persName>
                  <idno type="halauthorid">1555048</idno>
                  <affiliation ref="#struct-30303"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Martin</forename>
                    <surname>Schorb</surname>
                  </persName>
                  <idno type="halauthorid">1555049</idno>
                  <affiliation ref="#struct-30303"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Priit</forename>
                    <surname>Pruunsild</surname>
                  </persName>
                  <idno type="halauthorid">1555050</idno>
                  <affiliation ref="#struct-306189"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Yannick</forename>
                    <surname>Schwab</surname>
                  </persName>
                  <idno type="halauthorid">176745</idno>
                  <affiliation ref="#struct-30303"></affiliation>
                  <affiliation ref="#struct-134947"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Laurent</forename>
                    <surname>Chatel-Chaix</surname>
                  </persName>
                  <idno type="halauthorid">1555051</idno>
                  <affiliation ref="#struct-54664"></affiliation>
                  <affiliation ref="#struct-500956"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Alessia</forename>
                    <surname>Ruggieri</surname>
                  </persName>
                  <idno type="halauthorid">1555052</idno>
                  <affiliation ref="#struct-500956"></affiliation>
                </author>
                <author role="crp">                  <persName>                    <forename type="first">Ralf</forename>
                    <surname>Bartenschlager</surname>
                  </persName>
                  <email type="md5">9daba53b67ba715d99e7e84334eafe41</email>
                  <email type="domain">med.uni-heidelberg.de</email>
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                </author>
              </analytic>
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                <idno type="issn">2211-1247</idno>
                <idno type="eissn">2211-1247</idno>
                <title level="j">Cell Reports</title>
                <imprint>                  <publisher>Elsevier Inc</publisher>
                  <biblScope unit="volume">18</biblScope>
                  <biblScope unit="issue">9</biblScope>
                  <biblScope unit="pp">2113-2123</biblScope>
                  <date type="datePub">2017-02-28</date>
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              <idno type="doi">10.1016/j.celrep.2017.02.014</idno>
              <idno type="pubmed">28249158</idno>
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          <profileDesc>            <langUsage>              <language ident="en">English</language>
            </langUsage>
            <textClass>              <keywords scheme="author">                <term xml:lang="en">intermediate filaments</term>
                <term xml:lang="en">live-cell imaging</term>
                <term xml:lang="en">Zika virus</term>
                <term xml:lang="en">electron microscopy</term>
                <term xml:lang="en">electron tomography</term>
                <term xml:lang="en">flavivirus</term>
                <term xml:lang="en">human neural progenitor cells</term>
                <term xml:lang="en">microtubules</term>
                <term xml:lang="en">replication factories</term>
                <term xml:lang="en">replication organelles</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">              <p>A global concern has emerged with the pandemic spread of Zika virus (ZIKV) infections that can cause severe neurological symptoms in adults and newborns. ZIKV is a positive-strand RNA virus replicating in virus-induced membranous replication factories (RFs). Here we used various imaging techniques to investigate the ultrastructural details of ZIKV RFs and their relationship with host cell organelles. Analyses of human hepatic cells and neural progenitor cells infected with ZIKV revealed endoplasmic reticulum (ER) membrane invaginations containing pore-like openings toward the cytosol, reminiscent to RFs in Dengue virus-infected cells. Both the MR766 African strain and the H/PF/2013 Asian strain, the latter linked to neurological diseases, induce RFs of similar architecture. Importantly, ZIKV infection causes a drastic reorganization of microtubules and intermediate filaments forming cage-like structures surrounding the viral RF. Consistently, ZIKV replication is suppressed by cytoskeleton-targeting drugs. Thus, ZIKV RFs are tightly linked to rearrangements of the host cell cytoskeleton.</p>
            </abstract>
          </profileDesc>
        </hal>
</record>

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Ultrastructural Characterization of Zika Virus Replication Factories.

Ultrastructural Characterization of Zika Virus Replication Factories.

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