Ultrastructural Characterization of Zika Virus Replication Factories.
Identifieur interne : 000675 ( Hal/Corpus ); précédent : 000674; suivant : 000676Ultrastructural Characterization of Zika Virus Replication Factories.
Auteurs : Mirko Cortese ; Sarah Goellner ; Eliana Gisela Acosta ; Christopher John Neufeldt ; Olga Oleksiuk ; Marko Lampe ; Uta Haselmann ; Charlotta Funaya ; Nicole Schieber ; Paolo Ronchi ; Martin Schorb ; Priit Pruunsild ; Yannick Schwab ; Laurent Chatel-Chaix ; Alessia Ruggieri ; Ralf BartenschlagerSource :
- Cell Reports [ 2211-1247 ] ; 2017-02-28.
English descriptors
- mix :
Abstract
A global concern has emerged with the pandemic spread of Zika virus (ZIKV) infections that can cause severe neurological symptoms in adults and newborns. ZIKV is a positive-strand RNA virus replicating in virus-induced membranous replication factories (RFs). Here we used various imaging techniques to investigate the ultrastructural details of ZIKV RFs and their relationship with host cell organelles. Analyses of human hepatic cells and neural progenitor cells infected with ZIKV revealed endoplasmic reticulum (ER) membrane invaginations containing pore-like openings toward the cytosol, reminiscent to RFs in Dengue virus-infected cells. Both the MR766 African strain and the H/PF/2013 Asian strain, the latter linked to neurological diseases, induce RFs of similar architecture. Importantly, ZIKV infection causes a drastic reorganization of microtubules and intermediate filaments forming cage-like structures surrounding the viral RF. Consistently, ZIKV replication is suppressed by cytoskeleton-targeting drugs. Thus, ZIKV RFs are tightly linked to rearrangements of the host cell cytoskeleton.
Url:
DOI: 10.1016/j.celrep.2017.02.014
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Hal:pasteur-01535196Le document en format XML
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<front><div type="abstract" xml:lang="en"> <p>A global concern has emerged with the pandemic spread of Zika virus (ZIKV) infections that can cause severe neurological symptoms in adults and newborns. ZIKV is a positive-strand RNA virus replicating in virus-induced membranous replication factories (RFs). Here we used various imaging techniques to investigate the ultrastructural details of ZIKV RFs and their relationship with host cell organelles. Analyses of human hepatic cells and neural progenitor cells infected with ZIKV revealed endoplasmic reticulum (ER) membrane invaginations containing pore-like openings toward the cytosol, reminiscent to RFs in Dengue virus-infected cells. Both the MR766 African strain and the H/PF/2013 Asian strain, the latter linked to neurological diseases, induce RFs of similar architecture. Importantly, ZIKV infection causes a drastic reorganization of microtubules and intermediate filaments forming cage-like structures surrounding the viral RF. Consistently, ZIKV replication is suppressed by cytoskeleton-targeting drugs. Thus, ZIKV RFs are tightly linked to rearrangements of the host cell cytoskeleton.</p>
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<funder>This work was supported by grants from the Deutsche Forschungsgemeinschaft (SFB 1129 to R.B and A.R). L.C.-C. holds a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada (RGPIN-2016-05584). P.P. holds a fellowship by the Excellence Cluster Cell Networks at Heidelberg University. C.J.N is funded by a European Molecular Biology Organization (EMBO) Long-Term Fellowship (ALTF 466-2016) </funder>
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<abstract xml:lang="en"> <p>A global concern has emerged with the pandemic spread of Zika virus (ZIKV) infections that can cause severe neurological symptoms in adults and newborns. ZIKV is a positive-strand RNA virus replicating in virus-induced membranous replication factories (RFs). Here we used various imaging techniques to investigate the ultrastructural details of ZIKV RFs and their relationship with host cell organelles. Analyses of human hepatic cells and neural progenitor cells infected with ZIKV revealed endoplasmic reticulum (ER) membrane invaginations containing pore-like openings toward the cytosol, reminiscent to RFs in Dengue virus-infected cells. Both the MR766 African strain and the H/PF/2013 Asian strain, the latter linked to neurological diseases, induce RFs of similar architecture. Importantly, ZIKV infection causes a drastic reorganization of microtubules and intermediate filaments forming cage-like structures surrounding the viral RF. Consistently, ZIKV replication is suppressed by cytoskeleton-targeting drugs. Thus, ZIKV RFs are tightly linked to rearrangements of the host cell cytoskeleton.</p>
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