Corpus
Hal
Racine
Corpus
Checkpoint
Hal
Racine
Hal
Exploration
Accueil
Racine
Racine

Serveur d'exploration sur les pandémies grippales

Attention, ce site est en cours de développement !
Attention, site généré par des moyens informatiques à partir de corpus bruts.
Les informations ne sont donc pas validées.

Bacteriophage LM33_P1, a fast-acting weapon against the pandemic ST131-O25b:H4 Escherichia coli clonal complex.

Identifieur interne : 000071 ( Hal/Corpus ); précédent : 000070; suivant : 000072

Bacteriophage LM33_P1, a fast-acting weapon against the pandemic ST131-O25b:H4 Escherichia coli clonal complex.

Auteurs : Nicolas Dufour ; Olivier Clermont ; Béatrice La Combe ; Jonathan Messika ; Sara Dion ; Varun Khanna ; Erick Denamur ; Jean-Damien Ricard ; Laurent Debarbieux

Source :

RBID : Hal:pasteur-01539016

English descriptors

Abstract

OBJECTIVES:Amongst the highly diverse Escherichia coli population, the ST131-O25b:H4 clonal complex is particularly worrisome as it is associated with a high level of antibiotic resistance. The lack of new antibiotics, the worldwide continuous increase of infections caused by MDR bacteria and the need for narrow-spectrum antimicrobial agents have revived interest in phage therapy. In this article, we describe a virulent bacteriophage, LM33_P1, which specifically infects O25b strains, and provide data related to its therapeutic potential.METHODS:A large panel of E. coli strains (n = 283) was used to assess both the specificity and the activity of bacteriophage LM33_P1. Immunology, biochemistry and genetics-based methods confirmed this specificity. Virology methods and sequencing were used to characterize this bacteriophage in vitro, while three relevant mouse models were employed to show its in vivo efficacy.RESULTS: Bacteriophage LM33_P1 exclusively infects O25b E. coli strains with a 70% coverage on sequence types associated with high antibiotic resistance (ST131 and ST69). This specificity is due to an interaction with the LPS mediated by an original tail fibre. LM33_P1 also has exceptional intrinsic properties with a high adsorption constant and produces over 300 virions per cell in <10 min. Using animal pneumonia, septicaemia and urinary tract infection models, we showed the in vivo efficacy of LM33_P1 to reduce the bacterial load in several organs.CONCLUSION: Bacteriophage LM33_P1 represents the first weapon that specifically and quickly kills O25b E. coli strains. Therapeutic approaches derived from this bacteriophage could be developed to stop or slow down the spread of the ST131-O25b:H4 drug-resistant clonal complex in humans.


Url:
DOI: 10.1093/jac/dkw253

Links to Exploration step

Hal:pasteur-01539016

Le document en format XML

<record>
<TEI>
<teiHeader>
<fileDesc>
<titleStmt>
<title xml:lang="en">Bacteriophage LM33_P1, a fast-acting weapon against the pandemic ST131-O25b:H4 Escherichia coli clonal complex.</title>
<author>
<name sortKey="Dufour, Nicolas" sort="Dufour, Nicolas" uniqKey="Dufour N" first="Nicolas" last="Dufour">Nicolas Dufour</name>
<affiliation>
<hal:affiliation type="laboratory" xml:id="struct-28309" status="OLD">
<orgName>Biologie Moléculaire du Gène chez les Extrêmophiles</orgName>
<orgName type="acronym">BMGE</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>Département Microbiologie - 25-28 rue du Docteur Roux, F-75724 Paris Cedex 15</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://research.pasteur.fr/fr/team/molecular-biology-of-gene-in-extremophiles/</ref>
</desc>
<listRelation>
<relation active="#struct-300027" type="direct"></relation>
</listRelation>
<tutelles>
<tutelle active="#struct-300027" type="direct">
<org type="institution" xml:id="struct-300027" status="VALID">
<idno type="IdRef">027936643</idno>
<idno type="ISNI">0000 0001 2353 6535</idno>
<orgName>Institut Pasteur [Paris]</orgName>
<date type="start">1887-06-04</date>
<desc>
<address>
<addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://www.pasteur.fr</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Clermont, Olivier" sort="Clermont, Olivier" uniqKey="Clermont O" first="Olivier" last="Clermont">Olivier Clermont</name>
<affiliation>
<hal:affiliation type="laboratory" xml:id="struct-247341" status="OLD">
<idno type="RNSR">201420743R</idno>
<idno type="IdRef">235514829</idno>
<orgName>Infection, Anti-microbiens, Modélisation, Evolution</orgName>
<orgName type="acronym">IAME (UMR_S_1137 / U1137)</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>Faculté de médecine Paris Diderot Paris 7 - site Bichat - 16 rue Henri Huchard 75890 Paris Cedex 18</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://www.iame-research.center/</ref>
</desc>
<listRelation>
<relation active="#struct-15786" type="direct"></relation>
<relation name="UMR_S_1137" active="#struct-300301" type="direct"></relation>
<relation active="#struct-303171" type="direct"></relation>
<relation name="U1137" active="#struct-303623" type="direct"></relation>
</listRelation>
<tutelles>
<tutelle active="#struct-15786" type="direct">
<org type="institution" xml:id="struct-15786" status="VALID">
<orgName>Université Paris 13</orgName>
<orgName type="acronym">UP13</orgName>
<desc>
<address>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-paris13.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_S_1137" active="#struct-300301" type="direct">
<org type="institution" xml:id="struct-300301" status="OLD">
<idno type="ISNI">0000000121514068</idno>
<idno type="IdRef">027542084</idno>
<orgName>Université Paris Diderot - Paris 7</orgName>
<orgName type="acronym">UPD7</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-paris-diderot.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-303171" type="direct">
<org type="regroupinstitution" xml:id="struct-303171" status="VALID">
<orgName>Université Sorbonne Paris Cité</orgName>
<orgName type="acronym">USPC</orgName>
<date type="start">2010</date>
<desc>
<address>
<addrLine>Université Sorbonne Paris Cité | 100-104, avenue de France | 75013 Paris</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.sorbonne-paris-cite.fr/fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="U1137" active="#struct-303623" type="direct">
<org type="institution" xml:id="struct-303623" status="VALID">
<idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc>
<address>
<addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author>
<name sortKey="La Combe, Beatrice" sort="La Combe, Beatrice" uniqKey="La Combe B" first="Béatrice" last="La Combe">Béatrice La Combe</name>
<affiliation>
<hal:affiliation type="laboratory" xml:id="struct-247341" status="OLD">
<idno type="RNSR">201420743R</idno>
<idno type="IdRef">235514829</idno>
<orgName>Infection, Anti-microbiens, Modélisation, Evolution</orgName>
<orgName type="acronym">IAME (UMR_S_1137 / U1137)</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>Faculté de médecine Paris Diderot Paris 7 - site Bichat - 16 rue Henri Huchard 75890 Paris Cedex 18</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://www.iame-research.center/</ref>
</desc>
<listRelation>
<relation active="#struct-15786" type="direct"></relation>
<relation name="UMR_S_1137" active="#struct-300301" type="direct"></relation>
<relation active="#struct-303171" type="direct"></relation>
<relation name="U1137" active="#struct-303623" type="direct"></relation>
</listRelation>
<tutelles>
<tutelle active="#struct-15786" type="direct">
<org type="institution" xml:id="struct-15786" status="VALID">
<orgName>Université Paris 13</orgName>
<orgName type="acronym">UP13</orgName>
<desc>
<address>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-paris13.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_S_1137" active="#struct-300301" type="direct">
<org type="institution" xml:id="struct-300301" status="OLD">
<idno type="ISNI">0000000121514068</idno>
<idno type="IdRef">027542084</idno>
<orgName>Université Paris Diderot - Paris 7</orgName>
<orgName type="acronym">UPD7</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-paris-diderot.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-303171" type="direct">
<org type="regroupinstitution" xml:id="struct-303171" status="VALID">
<orgName>Université Sorbonne Paris Cité</orgName>
<orgName type="acronym">USPC</orgName>
<date type="start">2010</date>
<desc>
<address>
<addrLine>Université Sorbonne Paris Cité | 100-104, avenue de France | 75013 Paris</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.sorbonne-paris-cite.fr/fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="U1137" active="#struct-303623" type="direct">
<org type="institution" xml:id="struct-303623" status="VALID">
<idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc>
<address>
<addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Messika, Jonathan" sort="Messika, Jonathan" uniqKey="Messika J" first="Jonathan" last="Messika">Jonathan Messika</name>
<affiliation>
<hal:affiliation type="department" xml:id="struct-457457" status="VALID">
<orgName>Service de Réanimation Médico-Chirurgicale [Hôpital Louis Mourier]</orgName>
<desc>
<address>
<country key="FR"></country>
</address>
</desc>
<listRelation>
<relation active="#struct-300068" type="direct"></relation>
<relation active="#struct-344348" type="direct"></relation>
</listRelation>
<tutelles>
<tutelle active="#struct-300068" type="direct">
<org type="regroupinstitution" xml:id="struct-300068" status="VALID">
<orgName>Assistance publique - Hôpitaux de Paris (AP-HP)</orgName>
<orgName type="acronym">APHP</orgName>
<desc>
<address>
<country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
<tutelle active="#struct-344348" type="direct">
<org type="institution" xml:id="struct-344348" status="VALID">
<orgName>Hopital Louis Mourier - AP-HP [Colombes]</orgName>
<desc>
<address>
<addrLine>178 Rue des Renouillers, 92700 Colombes</addrLine>
<country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Dion, Sara" sort="Dion, Sara" uniqKey="Dion S" first="Sara" last="Dion">Sara Dion</name>
<affiliation>
<hal:affiliation type="laboratory" xml:id="struct-247341" status="OLD">
<idno type="RNSR">201420743R</idno>
<idno type="IdRef">235514829</idno>
<orgName>Infection, Anti-microbiens, Modélisation, Evolution</orgName>
<orgName type="acronym">IAME (UMR_S_1137 / U1137)</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>Faculté de médecine Paris Diderot Paris 7 - site Bichat - 16 rue Henri Huchard 75890 Paris Cedex 18</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://www.iame-research.center/</ref>
</desc>
<listRelation>
<relation active="#struct-15786" type="direct"></relation>
<relation name="UMR_S_1137" active="#struct-300301" type="direct"></relation>
<relation active="#struct-303171" type="direct"></relation>
<relation name="U1137" active="#struct-303623" type="direct"></relation>
</listRelation>
<tutelles>
<tutelle active="#struct-15786" type="direct">
<org type="institution" xml:id="struct-15786" status="VALID">
<orgName>Université Paris 13</orgName>
<orgName type="acronym">UP13</orgName>
<desc>
<address>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-paris13.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_S_1137" active="#struct-300301" type="direct">
<org type="institution" xml:id="struct-300301" status="OLD">
<idno type="ISNI">0000000121514068</idno>
<idno type="IdRef">027542084</idno>
<orgName>Université Paris Diderot - Paris 7</orgName>
<orgName type="acronym">UPD7</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-paris-diderot.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-303171" type="direct">
<org type="regroupinstitution" xml:id="struct-303171" status="VALID">
<orgName>Université Sorbonne Paris Cité</orgName>
<orgName type="acronym">USPC</orgName>
<date type="start">2010</date>
<desc>
<address>
<addrLine>Université Sorbonne Paris Cité | 100-104, avenue de France | 75013 Paris</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.sorbonne-paris-cite.fr/fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="U1137" active="#struct-303623" type="direct">
<org type="institution" xml:id="struct-303623" status="VALID">
<idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc>
<address>
<addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Khanna, Varun" sort="Khanna, Varun" uniqKey="Khanna V" first="Varun" last="Khanna">Varun Khanna</name>
<affiliation>
<hal:affiliation type="laboratory" xml:id="struct-463018" status="OLD">
<orgName>Centre de Bioinformatique, Biostatistique et Biologie Intégrative</orgName>
<orgName type="acronym">C3BI</orgName>
<date type="start">2015-03-01</date>
<date type="end">2019-04-30</date>
<desc>
<address>
<addrLine>25-28 rue du docteur Roux, 75724 Paris cedex 15</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://c3bi.pasteur.fr/</ref>
</desc>
<listRelation>
<relation active="#struct-300027" type="direct"></relation>
<relation name="USR3756" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles>
<tutelle active="#struct-300027" type="direct">
<org type="institution" xml:id="struct-300027" status="VALID">
<idno type="IdRef">027936643</idno>
<idno type="ISNI">0000 0001 2353 6535</idno>
<orgName>Institut Pasteur [Paris]</orgName>
<date type="start">1887-06-04</date>
<desc>
<address>
<addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://www.pasteur.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="USR3756" active="#struct-441569" type="direct">
<org type="institution" xml:id="struct-441569" status="VALID">
<idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc>
<address>
<country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Denamur, Erick" sort="Denamur, Erick" uniqKey="Denamur E" first="Erick" last="Denamur">Erick Denamur</name>
<affiliation>
<hal:affiliation type="institution" xml:id="struct-349749" status="INCOMING">
<orgName>Hôpitaux Universitaires Paris Nord Val de Seine</orgName>
<desc>
<address>
<country key="FR"></country>
</address>
</desc>
</hal:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Ricard, Jean Damien" sort="Ricard, Jean Damien" uniqKey="Ricard J" first="Jean-Damien" last="Ricard">Jean-Damien Ricard</name>
<affiliation>
<hal:affiliation type="laboratory" xml:id="struct-247341" status="OLD">
<idno type="RNSR">201420743R</idno>
<idno type="IdRef">235514829</idno>
<orgName>Infection, Anti-microbiens, Modélisation, Evolution</orgName>
<orgName type="acronym">IAME (UMR_S_1137 / U1137)</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>Faculté de médecine Paris Diderot Paris 7 - site Bichat - 16 rue Henri Huchard 75890 Paris Cedex 18</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://www.iame-research.center/</ref>
</desc>
<listRelation>
<relation active="#struct-15786" type="direct"></relation>
<relation name="UMR_S_1137" active="#struct-300301" type="direct"></relation>
<relation active="#struct-303171" type="direct"></relation>
<relation name="U1137" active="#struct-303623" type="direct"></relation>
</listRelation>
<tutelles>
<tutelle active="#struct-15786" type="direct">
<org type="institution" xml:id="struct-15786" status="VALID">
<orgName>Université Paris 13</orgName>
<orgName type="acronym">UP13</orgName>
<desc>
<address>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-paris13.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_S_1137" active="#struct-300301" type="direct">
<org type="institution" xml:id="struct-300301" status="OLD">
<idno type="ISNI">0000000121514068</idno>
<idno type="IdRef">027542084</idno>
<orgName>Université Paris Diderot - Paris 7</orgName>
<orgName type="acronym">UPD7</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-paris-diderot.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-303171" type="direct">
<org type="regroupinstitution" xml:id="struct-303171" status="VALID">
<orgName>Université Sorbonne Paris Cité</orgName>
<orgName type="acronym">USPC</orgName>
<date type="start">2010</date>
<desc>
<address>
<addrLine>Université Sorbonne Paris Cité | 100-104, avenue de France | 75013 Paris</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.sorbonne-paris-cite.fr/fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="U1137" active="#struct-303623" type="direct">
<org type="institution" xml:id="struct-303623" status="VALID">
<idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc>
<address>
<addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Debarbieux, Laurent" sort="Debarbieux, Laurent" uniqKey="Debarbieux L" first="Laurent" last="Debarbieux">Laurent Debarbieux</name>
<affiliation>
<hal:affiliation type="laboratory" xml:id="struct-28309" status="OLD">
<orgName>Biologie Moléculaire du Gène chez les Extrêmophiles</orgName>
<orgName type="acronym">BMGE</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>Département Microbiologie - 25-28 rue du Docteur Roux, F-75724 Paris Cedex 15</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://research.pasteur.fr/fr/team/molecular-biology-of-gene-in-extremophiles/</ref>
</desc>
<listRelation>
<relation active="#struct-300027" type="direct"></relation>
</listRelation>
<tutelles>
<tutelle active="#struct-300027" type="direct">
<org type="institution" xml:id="struct-300027" status="VALID">
<idno type="IdRef">027936643</idno>
<idno type="ISNI">0000 0001 2353 6535</idno>
<orgName>Institut Pasteur [Paris]</orgName>
<date type="start">1887-06-04</date>
<desc>
<address>
<addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://www.pasteur.fr</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
</titleStmt>
<publicationStmt>
<idno type="wicri:source">HAL</idno>
<idno type="RBID">Hal:pasteur-01539016</idno>
<idno type="halId">pasteur-01539016</idno>
<idno type="halUri">https://hal-pasteur.archives-ouvertes.fr/pasteur-01539016</idno>
<idno type="url">https://hal-pasteur.archives-ouvertes.fr/pasteur-01539016</idno>
<idno type="doi">10.1093/jac/dkw253</idno>
<date when="2016-11">2016-11</date>
<idno type="wicri:Area/Hal/Corpus">000071</idno>
</publicationStmt>
<sourceDesc>
<biblStruct>
<analytic>
<title xml:lang="en">Bacteriophage LM33_P1, a fast-acting weapon against the pandemic ST131-O25b:H4 Escherichia coli clonal complex.</title>
<author>
<name sortKey="Dufour, Nicolas" sort="Dufour, Nicolas" uniqKey="Dufour N" first="Nicolas" last="Dufour">Nicolas Dufour</name>
<affiliation>
<hal:affiliation type="laboratory" xml:id="struct-28309" status="OLD">
<orgName>Biologie Moléculaire du Gène chez les Extrêmophiles</orgName>
<orgName type="acronym">BMGE</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>Département Microbiologie - 25-28 rue du Docteur Roux, F-75724 Paris Cedex 15</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://research.pasteur.fr/fr/team/molecular-biology-of-gene-in-extremophiles/</ref>
</desc>
<listRelation>
<relation active="#struct-300027" type="direct"></relation>
</listRelation>
<tutelles>
<tutelle active="#struct-300027" type="direct">
<org type="institution" xml:id="struct-300027" status="VALID">
<idno type="IdRef">027936643</idno>
<idno type="ISNI">0000 0001 2353 6535</idno>
<orgName>Institut Pasteur [Paris]</orgName>
<date type="start">1887-06-04</date>
<desc>
<address>
<addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://www.pasteur.fr</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Clermont, Olivier" sort="Clermont, Olivier" uniqKey="Clermont O" first="Olivier" last="Clermont">Olivier Clermont</name>
<affiliation>
<hal:affiliation type="laboratory" xml:id="struct-247341" status="OLD">
<idno type="RNSR">201420743R</idno>
<idno type="IdRef">235514829</idno>
<orgName>Infection, Anti-microbiens, Modélisation, Evolution</orgName>
<orgName type="acronym">IAME (UMR_S_1137 / U1137)</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>Faculté de médecine Paris Diderot Paris 7 - site Bichat - 16 rue Henri Huchard 75890 Paris Cedex 18</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://www.iame-research.center/</ref>
</desc>
<listRelation>
<relation active="#struct-15786" type="direct"></relation>
<relation name="UMR_S_1137" active="#struct-300301" type="direct"></relation>
<relation active="#struct-303171" type="direct"></relation>
<relation name="U1137" active="#struct-303623" type="direct"></relation>
</listRelation>
<tutelles>
<tutelle active="#struct-15786" type="direct">
<org type="institution" xml:id="struct-15786" status="VALID">
<orgName>Université Paris 13</orgName>
<orgName type="acronym">UP13</orgName>
<desc>
<address>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-paris13.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_S_1137" active="#struct-300301" type="direct">
<org type="institution" xml:id="struct-300301" status="OLD">
<idno type="ISNI">0000000121514068</idno>
<idno type="IdRef">027542084</idno>
<orgName>Université Paris Diderot - Paris 7</orgName>
<orgName type="acronym">UPD7</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-paris-diderot.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-303171" type="direct">
<org type="regroupinstitution" xml:id="struct-303171" status="VALID">
<orgName>Université Sorbonne Paris Cité</orgName>
<orgName type="acronym">USPC</orgName>
<date type="start">2010</date>
<desc>
<address>
<addrLine>Université Sorbonne Paris Cité | 100-104, avenue de France | 75013 Paris</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.sorbonne-paris-cite.fr/fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="U1137" active="#struct-303623" type="direct">
<org type="institution" xml:id="struct-303623" status="VALID">
<idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc>
<address>
<addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author>
<name sortKey="La Combe, Beatrice" sort="La Combe, Beatrice" uniqKey="La Combe B" first="Béatrice" last="La Combe">Béatrice La Combe</name>
<affiliation>
<hal:affiliation type="laboratory" xml:id="struct-247341" status="OLD">
<idno type="RNSR">201420743R</idno>
<idno type="IdRef">235514829</idno>
<orgName>Infection, Anti-microbiens, Modélisation, Evolution</orgName>
<orgName type="acronym">IAME (UMR_S_1137 / U1137)</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>Faculté de médecine Paris Diderot Paris 7 - site Bichat - 16 rue Henri Huchard 75890 Paris Cedex 18</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://www.iame-research.center/</ref>
</desc>
<listRelation>
<relation active="#struct-15786" type="direct"></relation>
<relation name="UMR_S_1137" active="#struct-300301" type="direct"></relation>
<relation active="#struct-303171" type="direct"></relation>
<relation name="U1137" active="#struct-303623" type="direct"></relation>
</listRelation>
<tutelles>
<tutelle active="#struct-15786" type="direct">
<org type="institution" xml:id="struct-15786" status="VALID">
<orgName>Université Paris 13</orgName>
<orgName type="acronym">UP13</orgName>
<desc>
<address>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-paris13.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_S_1137" active="#struct-300301" type="direct">
<org type="institution" xml:id="struct-300301" status="OLD">
<idno type="ISNI">0000000121514068</idno>
<idno type="IdRef">027542084</idno>
<orgName>Université Paris Diderot - Paris 7</orgName>
<orgName type="acronym">UPD7</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-paris-diderot.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-303171" type="direct">
<org type="regroupinstitution" xml:id="struct-303171" status="VALID">
<orgName>Université Sorbonne Paris Cité</orgName>
<orgName type="acronym">USPC</orgName>
<date type="start">2010</date>
<desc>
<address>
<addrLine>Université Sorbonne Paris Cité | 100-104, avenue de France | 75013 Paris</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.sorbonne-paris-cite.fr/fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="U1137" active="#struct-303623" type="direct">
<org type="institution" xml:id="struct-303623" status="VALID">
<idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc>
<address>
<addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Messika, Jonathan" sort="Messika, Jonathan" uniqKey="Messika J" first="Jonathan" last="Messika">Jonathan Messika</name>
<affiliation>
<hal:affiliation type="department" xml:id="struct-457457" status="VALID">
<orgName>Service de Réanimation Médico-Chirurgicale [Hôpital Louis Mourier]</orgName>
<desc>
<address>
<country key="FR"></country>
</address>
</desc>
<listRelation>
<relation active="#struct-300068" type="direct"></relation>
<relation active="#struct-344348" type="direct"></relation>
</listRelation>
<tutelles>
<tutelle active="#struct-300068" type="direct">
<org type="regroupinstitution" xml:id="struct-300068" status="VALID">
<orgName>Assistance publique - Hôpitaux de Paris (AP-HP)</orgName>
<orgName type="acronym">APHP</orgName>
<desc>
<address>
<country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
<tutelle active="#struct-344348" type="direct">
<org type="institution" xml:id="struct-344348" status="VALID">
<orgName>Hopital Louis Mourier - AP-HP [Colombes]</orgName>
<desc>
<address>
<addrLine>178 Rue des Renouillers, 92700 Colombes</addrLine>
<country key="FR"></country>
</address>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Dion, Sara" sort="Dion, Sara" uniqKey="Dion S" first="Sara" last="Dion">Sara Dion</name>
<affiliation>
<hal:affiliation type="laboratory" xml:id="struct-247341" status="OLD">
<idno type="RNSR">201420743R</idno>
<idno type="IdRef">235514829</idno>
<orgName>Infection, Anti-microbiens, Modélisation, Evolution</orgName>
<orgName type="acronym">IAME (UMR_S_1137 / U1137)</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>Faculté de médecine Paris Diderot Paris 7 - site Bichat - 16 rue Henri Huchard 75890 Paris Cedex 18</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://www.iame-research.center/</ref>
</desc>
<listRelation>
<relation active="#struct-15786" type="direct"></relation>
<relation name="UMR_S_1137" active="#struct-300301" type="direct"></relation>
<relation active="#struct-303171" type="direct"></relation>
<relation name="U1137" active="#struct-303623" type="direct"></relation>
</listRelation>
<tutelles>
<tutelle active="#struct-15786" type="direct">
<org type="institution" xml:id="struct-15786" status="VALID">
<orgName>Université Paris 13</orgName>
<orgName type="acronym">UP13</orgName>
<desc>
<address>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-paris13.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_S_1137" active="#struct-300301" type="direct">
<org type="institution" xml:id="struct-300301" status="OLD">
<idno type="ISNI">0000000121514068</idno>
<idno type="IdRef">027542084</idno>
<orgName>Université Paris Diderot - Paris 7</orgName>
<orgName type="acronym">UPD7</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-paris-diderot.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-303171" type="direct">
<org type="regroupinstitution" xml:id="struct-303171" status="VALID">
<orgName>Université Sorbonne Paris Cité</orgName>
<orgName type="acronym">USPC</orgName>
<date type="start">2010</date>
<desc>
<address>
<addrLine>Université Sorbonne Paris Cité | 100-104, avenue de France | 75013 Paris</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.sorbonne-paris-cite.fr/fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="U1137" active="#struct-303623" type="direct">
<org type="institution" xml:id="struct-303623" status="VALID">
<idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc>
<address>
<addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Khanna, Varun" sort="Khanna, Varun" uniqKey="Khanna V" first="Varun" last="Khanna">Varun Khanna</name>
<affiliation>
<hal:affiliation type="laboratory" xml:id="struct-463018" status="OLD">
<orgName>Centre de Bioinformatique, Biostatistique et Biologie Intégrative</orgName>
<orgName type="acronym">C3BI</orgName>
<date type="start">2015-03-01</date>
<date type="end">2019-04-30</date>
<desc>
<address>
<addrLine>25-28 rue du docteur Roux, 75724 Paris cedex 15</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://c3bi.pasteur.fr/</ref>
</desc>
<listRelation>
<relation active="#struct-300027" type="direct"></relation>
<relation name="USR3756" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles>
<tutelle active="#struct-300027" type="direct">
<org type="institution" xml:id="struct-300027" status="VALID">
<idno type="IdRef">027936643</idno>
<idno type="ISNI">0000 0001 2353 6535</idno>
<orgName>Institut Pasteur [Paris]</orgName>
<date type="start">1887-06-04</date>
<desc>
<address>
<addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://www.pasteur.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="USR3756" active="#struct-441569" type="direct">
<org type="institution" xml:id="struct-441569" status="VALID">
<idno type="IdRef">02636817X</idno>
<idno type="ISNI">0000000122597504</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc>
<address>
<country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Denamur, Erick" sort="Denamur, Erick" uniqKey="Denamur E" first="Erick" last="Denamur">Erick Denamur</name>
<affiliation>
<hal:affiliation type="institution" xml:id="struct-349749" status="INCOMING">
<orgName>Hôpitaux Universitaires Paris Nord Val de Seine</orgName>
<desc>
<address>
<country key="FR"></country>
</address>
</desc>
</hal:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Ricard, Jean Damien" sort="Ricard, Jean Damien" uniqKey="Ricard J" first="Jean-Damien" last="Ricard">Jean-Damien Ricard</name>
<affiliation>
<hal:affiliation type="laboratory" xml:id="struct-247341" status="OLD">
<idno type="RNSR">201420743R</idno>
<idno type="IdRef">235514829</idno>
<orgName>Infection, Anti-microbiens, Modélisation, Evolution</orgName>
<orgName type="acronym">IAME (UMR_S_1137 / U1137)</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>Faculté de médecine Paris Diderot Paris 7 - site Bichat - 16 rue Henri Huchard 75890 Paris Cedex 18</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://www.iame-research.center/</ref>
</desc>
<listRelation>
<relation active="#struct-15786" type="direct"></relation>
<relation name="UMR_S_1137" active="#struct-300301" type="direct"></relation>
<relation active="#struct-303171" type="direct"></relation>
<relation name="U1137" active="#struct-303623" type="direct"></relation>
</listRelation>
<tutelles>
<tutelle active="#struct-15786" type="direct">
<org type="institution" xml:id="struct-15786" status="VALID">
<orgName>Université Paris 13</orgName>
<orgName type="acronym">UP13</orgName>
<desc>
<address>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-paris13.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_S_1137" active="#struct-300301" type="direct">
<org type="institution" xml:id="struct-300301" status="OLD">
<idno type="ISNI">0000000121514068</idno>
<idno type="IdRef">027542084</idno>
<orgName>Université Paris Diderot - Paris 7</orgName>
<orgName type="acronym">UPD7</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>5 rue Thomas-Mann - 75205 Paris cedex 13</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-paris-diderot.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-303171" type="direct">
<org type="regroupinstitution" xml:id="struct-303171" status="VALID">
<orgName>Université Sorbonne Paris Cité</orgName>
<orgName type="acronym">USPC</orgName>
<date type="start">2010</date>
<desc>
<address>
<addrLine>Université Sorbonne Paris Cité | 100-104, avenue de France | 75013 Paris</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.sorbonne-paris-cite.fr/fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="U1137" active="#struct-303623" type="direct">
<org type="institution" xml:id="struct-303623" status="VALID">
<idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc>
<address>
<addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Debarbieux, Laurent" sort="Debarbieux, Laurent" uniqKey="Debarbieux L" first="Laurent" last="Debarbieux">Laurent Debarbieux</name>
<affiliation>
<hal:affiliation type="laboratory" xml:id="struct-28309" status="OLD">
<orgName>Biologie Moléculaire du Gène chez les Extrêmophiles</orgName>
<orgName type="acronym">BMGE</orgName>
<date type="end">2019-12-31</date>
<desc>
<address>
<addrLine>Département Microbiologie - 25-28 rue du Docteur Roux, F-75724 Paris Cedex 15</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://research.pasteur.fr/fr/team/molecular-biology-of-gene-in-extremophiles/</ref>
</desc>
<listRelation>
<relation active="#struct-300027" type="direct"></relation>
</listRelation>
<tutelles>
<tutelle active="#struct-300027" type="direct">
<org type="institution" xml:id="struct-300027" status="VALID">
<idno type="IdRef">027936643</idno>
<idno type="ISNI">0000 0001 2353 6535</idno>
<orgName>Institut Pasteur [Paris]</orgName>
<date type="start">1887-06-04</date>
<desc>
<address>
<addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://www.pasteur.fr</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
</analytic>
<idno type="DOI">10.1093/jac/dkw253</idno>
<series>
<title level="j">Journal of Antimicrobial Chemotherapy</title>
<idno type="ISSN">0305-7453</idno>
<imprint>
<date type="datePub">2016-11</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc>
<textClass>
<keywords scheme="mix" xml:lang="en">
<term>23 bacteriophage</term>
<term>Escherichia coli</term>
<term>T131 -O25b:H4</term>
<term>antibiotic resistance</term>
<term>extended spectrum beta-lactamase</term>
<term>phage therapy</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front>
<div type="abstract" xml:lang="0">
<p>OBJECTIVES:Amongst the highly diverse Escherichia coli population, the ST131-O25b:H4 clonal complex is particularly worrisome as it is associated with a high level of antibiotic resistance. The lack of new antibiotics, the worldwide continuous increase of infections caused by MDR bacteria and the need for narrow-spectrum antimicrobial agents have revived interest in phage therapy. In this article, we describe a virulent bacteriophage, LM33_P1, which specifically infects O25b strains, and provide data related to its therapeutic potential.METHODS:A large panel of E. coli strains (n = 283) was used to assess both the specificity and the activity of bacteriophage LM33_P1. Immunology, biochemistry and genetics-based methods confirmed this specificity. Virology methods and sequencing were used to characterize this bacteriophage in vitro, while three relevant mouse models were employed to show its in vivo efficacy.RESULTS: Bacteriophage LM33_P1 exclusively infects O25b E. coli strains with a 70% coverage on sequence types associated with high antibiotic resistance (ST131 and ST69). This specificity is due to an interaction with the LPS mediated by an original tail fibre. LM33_P1 also has exceptional intrinsic properties with a high adsorption constant and produces over 300 virions per cell in <10 min. Using animal pneumonia, septicaemia and urinary tract infection models, we showed the in vivo efficacy of LM33_P1 to reduce the bacterial load in several organs.CONCLUSION: Bacteriophage LM33_P1 represents the first weapon that specifically and quickly kills O25b E. coli strains. Therapeutic approaches derived from this bacteriophage could be developed to stop or slow down the spread of the ST131-O25b:H4 drug-resistant clonal complex in humans.</p>
</div>
</front>
</TEI>
<hal api="V3">
<titleStmt>
<title xml:lang="en">Bacteriophage LM33_P1, a fast-acting weapon against the pandemic ST131-O25b:H4 Escherichia coli clonal complex.</title>
<author role="aut">
<persName>
<forename type="first">Nicolas</forename>
<surname>Dufour</surname>
</persName>
<idno type="halauthorid">994201</idno>
<affiliation ref="#struct-28309"></affiliation>
<affiliation ref="#struct-247341"></affiliation>
<affiliation ref="#struct-457457"></affiliation>
</author>
<author role="aut">
<persName>
<forename type="first">Olivier</forename>
<surname>Clermont</surname>
</persName>
<idno type="halauthorid">408953</idno>
<affiliation ref="#struct-247341"></affiliation>
</author>
<author role="aut">
<persName>
<forename type="first">Béatrice</forename>
<surname>La Combe</surname>
</persName>
<idno type="halauthorid">1558587</idno>
<affiliation ref="#struct-247341"></affiliation>
<affiliation ref="#struct-457457"></affiliation>
</author>
<author role="aut">
<persName>
<forename type="first">Jonathan</forename>
<surname>Messika</surname>
</persName>
<idno type="halauthorid">1235513</idno>
<affiliation ref="#struct-457457"></affiliation>
<affiliation ref="#struct-247341"></affiliation>
</author>
<author role="aut">
<persName>
<forename type="first">Sara</forename>
<surname>Dion</surname>
</persName>
<idno type="halauthorid">397005</idno>
<affiliation ref="#struct-247341"></affiliation>
</author>
<author role="aut">
<persName>
<forename type="first">Varun</forename>
<surname>Khanna</surname>
</persName>
<idno type="halauthorid">1128107</idno>
<affiliation ref="#struct-463018"></affiliation>
</author>
<author role="aut">
<persName>
<forename type="first">Erick</forename>
<surname>Denamur</surname>
</persName>
<idno type="halauthorid">566790</idno>
<affiliation ref="#struct-349749"></affiliation>
<affiliation ref="#struct-247341"></affiliation>
</author>
<author role="aut">
<persName>
<forename type="first">Jean-Damien</forename>
<surname>Ricard</surname>
</persName>
<idno type="halauthorid">467405</idno>
<affiliation ref="#struct-247341"></affiliation>
<affiliation ref="#struct-457457"></affiliation>
</author>
<author role="crp">
<persName>
<forename type="first">Laurent</forename>
<surname>Debarbieux</surname>
</persName>
<email type="md5">57d04d2b8f1e414bb238ff02e0295383</email>
<email type="domain">pasteur.fr</email>
<idno type="idhal" notation="string">laurent-debarbieux</idno>
<idno type="idhal" notation="numeric">14176</idno>
<idno type="halauthorid">1251013</idno>
<idno type="ORCID">https://orcid.org/0000-0001-6875-5758</idno>
<affiliation ref="#struct-28309"></affiliation>
</author>
<editor role="depositor">
<persName>
<forename>Laurent</forename>
<surname>Debarbieux</surname>
</persName>
<email type="md5">57d04d2b8f1e414bb238ff02e0295383</email>
<email type="domain">pasteur.fr</email>
</editor>
<funder>This project was supported by a joint research grant from both Institut Pasteur and Assistance Publique–Hôpitaux de Paris (Programme Transversal de Recherche #417 and Poste d′Accueil pour praticien hospitalier).</funder>
</titleStmt>
<editionStmt>
<edition n="v1" type="current">
<date type="whenSubmitted">2017-06-14 12:01:14</date>
<date type="whenModified">2020-04-09 11:57:52</date>
<date type="whenReleased">2017-06-19 14:51:37</date>
<date type="whenProduced">2016-11</date>
<date type="whenEndEmbargoed">2017-07-08</date>
<ref type="file" target="https://hal-pasteur.archives-ouvertes.fr/pasteur-01539016/document">
<date notBefore="2017-07-08"></date>
</ref>
<ref type="file" subtype="author" n="1" target="https://hal-pasteur.archives-ouvertes.fr/pasteur-01539016/file/Dufour-FinHal.pdf">
<date notBefore="2017-07-08"></date>
</ref>
<ref type="externalLink" target="https://academic.oup.com/jac/article-pdf/71/11/3072/16863860/dkw253.pdf"></ref>
</edition>
<respStmt>
<resp>contributor</resp>
<name key="162902">
<persName>
<forename>Laurent</forename>
<surname>Debarbieux</surname>
</persName>
<email type="md5">57d04d2b8f1e414bb238ff02e0295383</email>
<email type="domain">pasteur.fr</email>
</name>
</respStmt>
</editionStmt>
<publicationStmt>
<distributor>CCSD</distributor>
<idno type="halId">pasteur-01539016</idno>
<idno type="halUri">https://hal-pasteur.archives-ouvertes.fr/pasteur-01539016</idno>
<idno type="halBibtex">dufour:pasteur-01539016</idno>
<idno type="halRefHtml">Journal of Antimicrobial Chemotherapy, Oxford University Press (OUP), 2016, 71 (11), pp.3072-3080. ⟨10.1093/jac/dkw253⟩</idno>
<idno type="halRef">Journal of Antimicrobial Chemotherapy, Oxford University Press (OUP), 2016, 71 (11), pp.3072-3080. ⟨10.1093/jac/dkw253⟩</idno>
<availability status="restricted">
<licence target="http://creativecommons.org/licenses/by-nc-sa/">Attribution - NonCommercial - ShareAlike</licence>
</availability>
</publicationStmt>
<seriesStmt>
<idno type="stamp" n="PASTEUR">Institut Pasteur</idno>
<idno type="stamp" n="UNIV-PARIS7" corresp="UNIV-PARIS">Université Denis Diderot - Paris VII</idno>
<idno type="stamp" n="APHP" corresp="INSERM">AP-HP</idno>
<idno type="stamp" n="UNIV-PARIS13">Université Paris-Nord - Paris XIII </idno>
<idno type="stamp" n="USPC">Université Sorbonne Paris Cité</idno>
<idno type="stamp" n="INSERM">INSERM - Institut national de la santé et de la recherche médicale</idno>
<idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
<idno type="stamp" n="IAME" corresp="SORBONNE-PARIS-NORD">(Infection, Antimicrobiens, Modélisation, Evolution) - UMR 1137</idno>
<idno type="stamp" n="SORBONNE-PARIS-NORD">Université Sorbonne Paris Nord</idno>
<idno type="stamp" n="UNIV-PARIS">Université de Paris</idno>
<idno type="stamp" n="UP-SANTE">Université de Paris - Faculté de Santé</idno>
</seriesStmt>
<notesStmt>
<note type="audience" n="2">International</note>
<note type="popular" n="0">No</note>
<note type="peer" n="1">Yes</note>
</notesStmt>
<sourceDesc>
<biblStruct>
<analytic>
<title xml:lang="en">Bacteriophage LM33_P1, a fast-acting weapon against the pandemic ST131-O25b:H4 Escherichia coli clonal complex.</title>
<author role="aut">
<persName>
<forename type="first">Nicolas</forename>
<surname>Dufour</surname>
</persName>
<idno type="halauthorid">994201</idno>
<affiliation ref="#struct-28309"></affiliation>
<affiliation ref="#struct-247341"></affiliation>
<affiliation ref="#struct-457457"></affiliation>
</author>
<author role="aut">
<persName>
<forename type="first">Olivier</forename>
<surname>Clermont</surname>
</persName>
<idno type="halauthorid">408953</idno>
<affiliation ref="#struct-247341"></affiliation>
</author>
<author role="aut">
<persName>
<forename type="first">Béatrice</forename>
<surname>La Combe</surname>
</persName>
<idno type="halauthorid">1558587</idno>
<affiliation ref="#struct-247341"></affiliation>
<affiliation ref="#struct-457457"></affiliation>
</author>
<author role="aut">
<persName>
<forename type="first">Jonathan</forename>
<surname>Messika</surname>
</persName>
<idno type="halauthorid">1235513</idno>
<affiliation ref="#struct-457457"></affiliation>
<affiliation ref="#struct-247341"></affiliation>
</author>
<author role="aut">
<persName>
<forename type="first">Sara</forename>
<surname>Dion</surname>
</persName>
<idno type="halauthorid">397005</idno>
<affiliation ref="#struct-247341"></affiliation>
</author>
<author role="aut">
<persName>
<forename type="first">Varun</forename>
<surname>Khanna</surname>
</persName>
<idno type="halauthorid">1128107</idno>
<affiliation ref="#struct-463018"></affiliation>
</author>
<author role="aut">
<persName>
<forename type="first">Erick</forename>
<surname>Denamur</surname>
</persName>
<idno type="halauthorid">566790</idno>
<affiliation ref="#struct-349749"></affiliation>
<affiliation ref="#struct-247341"></affiliation>
</author>
<author role="aut">
<persName>
<forename type="first">Jean-Damien</forename>
<surname>Ricard</surname>
</persName>
<idno type="halauthorid">467405</idno>
<affiliation ref="#struct-247341"></affiliation>
<affiliation ref="#struct-457457"></affiliation>
</author>
<author role="crp">
<persName>
<forename type="first">Laurent</forename>
<surname>Debarbieux</surname>
</persName>
<email type="md5">57d04d2b8f1e414bb238ff02e0295383</email>
<email type="domain">pasteur.fr</email>
<idno type="idhal" notation="string">laurent-debarbieux</idno>
<idno type="idhal" notation="numeric">14176</idno>
<idno type="halauthorid">1251013</idno>
<idno type="ORCID">https://orcid.org/0000-0001-6875-5758</idno>
<affiliation ref="#struct-28309"></affiliation>
</author>
</analytic>
<monogr>
<idno type="halJournalId" status="VALID">15068</idno>
<idno type="issn">0305-7453</idno>
<idno type="eissn">1460-2091</idno>
<title level="j">Journal of Antimicrobial Chemotherapy</title>
<imprint>
<publisher>Oxford University Press (OUP)</publisher>
<biblScope unit="volume">71</biblScope>
<biblScope unit="issue">11</biblScope>
<biblScope unit="pp">3072-3080</biblScope>
<date type="datePub">2016-11</date>
<date type="dateEpub">2016-07-07</date>
</imprint>
</monogr>
<idno type="doi">10.1093/jac/dkw253</idno>
<idno type="pubmed">27387322</idno>
</biblStruct>
</sourceDesc>
<profileDesc>
<langUsage>
<language ident="en">English</language>
</langUsage>
<textClass>
<keywords scheme="author">
<term xml:lang="en">T131 -O25b:H4</term>
<term xml:lang="en">Escherichia coli</term>
<term xml:lang="en">antibiotic resistance</term>
<term xml:lang="en">phage therapy</term>
<term xml:lang="en">23 bacteriophage</term>
<term xml:lang="en">extended spectrum beta-lactamase</term>
</keywords>
<classCode scheme="halDomain" n="sdv.mp.bac">Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology</classCode>
<classCode scheme="halTypology" n="ART">Journal articles</classCode>
</textClass>
<abstract xml:lang="0">
<p>OBJECTIVES:Amongst the highly diverse Escherichia coli population, the ST131-O25b:H4 clonal complex is particularly worrisome as it is associated with a high level of antibiotic resistance. The lack of new antibiotics, the worldwide continuous increase of infections caused by MDR bacteria and the need for narrow-spectrum antimicrobial agents have revived interest in phage therapy. In this article, we describe a virulent bacteriophage, LM33_P1, which specifically infects O25b strains, and provide data related to its therapeutic potential.METHODS:A large panel of E. coli strains (n = 283) was used to assess both the specificity and the activity of bacteriophage LM33_P1. Immunology, biochemistry and genetics-based methods confirmed this specificity. Virology methods and sequencing were used to characterize this bacteriophage in vitro, while three relevant mouse models were employed to show its in vivo efficacy.RESULTS: Bacteriophage LM33_P1 exclusively infects O25b E. coli strains with a 70% coverage on sequence types associated with high antibiotic resistance (ST131 and ST69). This specificity is due to an interaction with the LPS mediated by an original tail fibre. LM33_P1 also has exceptional intrinsic properties with a high adsorption constant and produces over 300 virions per cell in <10 min. Using animal pneumonia, septicaemia and urinary tract infection models, we showed the in vivo efficacy of LM33_P1 to reduce the bacterial load in several organs.CONCLUSION: Bacteriophage LM33_P1 represents the first weapon that specifically and quickly kills O25b E. coli strains. Therapeutic approaches derived from this bacteriophage could be developed to stop or slow down the spread of the ST131-O25b:H4 drug-resistant clonal complex in humans.</p>
</abstract>
</profileDesc>
</hal>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/PandemieGrippaleV1/Data/Hal/Corpus

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000071 | SxmlIndent | more

Ou

HfdSelect -h $EXPLOR_AREA/Data/Hal/Corpus/biblio.hfd -nk 000071 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    PandemieGrippaleV1
   |flux=    Hal
   |étape=   Corpus
   |type=    RBID
   |clé=     Hal:pasteur-01539016
   |texte=   Bacteriophage LM33_P1, a fast-acting weapon against the pandemic ST131-O25b:H4 Escherichia coli clonal complex.
}}
Wicri

This area was generated with Dilib version V0.6.34.
Data generation: Wed Jun 10 11:04:28 2020. Site generation: Sun Mar 28 09:10:28 2021