PandemieGrippaleV1, Hal, Checkpoint, bibRecord, 000375

Detection of Escherichia coli sequence type 131 by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: implications for infection control policies?

Identifieur interne : 000375 ( Hal/Checkpoint ); précédent : 000374; suivant : 000376

Detection of Escherichia coli sequence type 131 by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: implications for infection control policies?

Auteurs : J. Lafolie ; M. Sauget [France] ; N. Cabrolier ; D. Hocquet [France] ; X. Bertrand [France]

Source :

The Journal of hospital infection [ 1532-2939 ] ; 2015-06-30.

RBID : Hal:hal-01306067

Abstract

Sequence type 131 (ST131) is a predominant lineage among extraintestinal pathogenic Escherichia coli. It plays a major role in the worldwide dissemination of extended-spectrum β-lactamase (ESBL)-producing E. coli. The ST131 pandemic is mainly the result of clonal expansion of the single well-adapted subclone H30-Rx, which is acquired in hospitals more frequently than other ESBL-producing E. coli clones. To develop a rapid method using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify ST131 for infection control purposes. Peak biomarkers of ST131 were identified from the mass spectrum profiles of 109 E. coli isolates (including 50 ST131 isolates). The models accurately identified ST131 isolates from mass spectrum profiles obtained with and without protein extraction. The rapid identification of ST131 isolates with MALDI-TOF MS can be easily implemented in the laboratory, and could help to target infection control measures in patients carrying multi-drug-resistant E. coli that are more likely to spread.

Url:

https://hal.archives-ouvertes.fr/hal-01306067

Links toward previous steps (curation, corpus...)

to stream Hal, to step Corpus: 000149
to stream Hal, to step Curation: 000149

Links to Exploration step

Hal:hal-01306067

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Detection of Escherichia coli sequence type 131 by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: implications for infection control policies?</title>
<author><name sortKey="Lafolie, J" sort="Lafolie, J" uniqKey="Lafolie J" first="J" last="Lafolie">J. Lafolie</name>
</author>
<author><name sortKey="Sauget, M" sort="Sauget, M" uniqKey="Sauget M" first="M" last="Sauget">M. Sauget</name>
<affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-300165" status="VALID">          <orgName>Centre Hospitalier Régional Universitaire [Besançon]</orgName>
          <orgName type="acronym">CHRU Besançon</orgName>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.chu-besancon.fr/</ref>
          </desc>
        </hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Cabrolier, N" sort="Cabrolier, N" uniqKey="Cabrolier N" first="N" last="Cabrolier">N. Cabrolier</name>
</author>
<author><name sortKey="Hocquet, D" sort="Hocquet, D" uniqKey="Hocquet D" first="D" last="Hocquet">D. Hocquet</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-57629" status="VALID">          <idno type="IdRef">166212377</idno>
          <idno type="RNSR">200812285Y</idno>
          <orgName>Laboratoire Chrono-environnement - UFC (UMR 6249)</orgName>
          <orgName type="acronym">LCE</orgName>
          <desc>            <address>              <addrLine>UFR Sciences et Techniques - 16, route de Gray - 25030 Besançon Cedex</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://chrono-environnement.univ-fcomte.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-426438" type="direct"></relation>
            <relation name="UMR6249" active="#struct-441569" type="direct"></relation>
            <relation active="#struct-458810" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle active="#struct-426438" type="direct"><org type="regroupinstitution" xml:id="struct-426438" status="VALID">          <idno type="IdRef">200716271</idno>
          <orgName>Université Bourgogne Franche-Comté [COMUE]</orgName>
          <orgName type="acronym">UBFC</orgName>
          <date type="start">2015-04-01</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ubfc.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR6249" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-458810" type="direct"><org type="institution" xml:id="struct-458810" status="VALID">          <idno type="IdRef">026403188</idno>
          <idno type="ISNI">0000 0001 2188 3779 </idno>
          <orgName>Université de Franche-Comté</orgName>
          <orgName type="acronym">UFC</orgName>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-fcomte.fr</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city" wicri:auto="siege">Besançon</settlement>
<region type="region" nuts="2">Franche-Comté</region>
</placeName>
<orgName type="university">Université de Franche-Comté</orgName>
<orgName type="institution" wicri:auto="newGroup">Université de Bourgogne Franche-Comté</orgName>
</affiliation>
</author>
<author><name sortKey="Bertrand, X" sort="Bertrand, X" uniqKey="Bertrand X" first="X" last="Bertrand">X. Bertrand</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-57629" status="VALID">          <idno type="IdRef">166212377</idno>
          <idno type="RNSR">200812285Y</idno>
          <orgName>Laboratoire Chrono-environnement - UFC (UMR 6249)</orgName>
          <orgName type="acronym">LCE</orgName>
          <desc>            <address>              <addrLine>UFR Sciences et Techniques - 16, route de Gray - 25030 Besançon Cedex</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://chrono-environnement.univ-fcomte.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-426438" type="direct"></relation>
            <relation name="UMR6249" active="#struct-441569" type="direct"></relation>
            <relation active="#struct-458810" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle active="#struct-426438" type="direct"><org type="regroupinstitution" xml:id="struct-426438" status="VALID">          <idno type="IdRef">200716271</idno>
          <orgName>Université Bourgogne Franche-Comté [COMUE]</orgName>
          <orgName type="acronym">UBFC</orgName>
          <date type="start">2015-04-01</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ubfc.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR6249" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-458810" type="direct"><org type="institution" xml:id="struct-458810" status="VALID">          <idno type="IdRef">026403188</idno>
          <idno type="ISNI">0000 0001 2188 3779 </idno>
          <orgName>Université de Franche-Comté</orgName>
          <orgName type="acronym">UFC</orgName>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-fcomte.fr</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city" wicri:auto="siege">Besançon</settlement>
<region type="region" nuts="2">Franche-Comté</region>
</placeName>
<orgName type="university">Université de Franche-Comté</orgName>
<orgName type="institution" wicri:auto="newGroup">Université de Bourgogne Franche-Comté</orgName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">HAL</idno>
<idno type="RBID">Hal:hal-01306067</idno>
<idno type="halId">hal-01306067</idno>
<idno type="halUri">https://hal.archives-ouvertes.fr/hal-01306067</idno>
<idno type="url">https://hal.archives-ouvertes.fr/hal-01306067</idno>
<date when="2015-06-30">2015-06-30</date>
<idno type="wicri:Area/Hal/Corpus">000149</idno>
<idno type="wicri:Area/Hal/Curation">000149</idno>
<idno type="wicri:Area/Hal/Checkpoint">000375</idno>
<idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000375</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Detection of Escherichia coli sequence type 131 by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: implications for infection control policies?</title>
<author><name sortKey="Lafolie, J" sort="Lafolie, J" uniqKey="Lafolie J" first="J" last="Lafolie">J. Lafolie</name>
</author>
<author><name sortKey="Sauget, M" sort="Sauget, M" uniqKey="Sauget M" first="M" last="Sauget">M. Sauget</name>
<affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-300165" status="VALID">          <orgName>Centre Hospitalier Régional Universitaire [Besançon]</orgName>
          <orgName type="acronym">CHRU Besançon</orgName>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.chu-besancon.fr/</ref>
          </desc>
        </hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Cabrolier, N" sort="Cabrolier, N" uniqKey="Cabrolier N" first="N" last="Cabrolier">N. Cabrolier</name>
</author>
<author><name sortKey="Hocquet, D" sort="Hocquet, D" uniqKey="Hocquet D" first="D" last="Hocquet">D. Hocquet</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-57629" status="VALID">          <idno type="IdRef">166212377</idno>
          <idno type="RNSR">200812285Y</idno>
          <orgName>Laboratoire Chrono-environnement - UFC (UMR 6249)</orgName>
          <orgName type="acronym">LCE</orgName>
          <desc>            <address>              <addrLine>UFR Sciences et Techniques - 16, route de Gray - 25030 Besançon Cedex</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://chrono-environnement.univ-fcomte.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-426438" type="direct"></relation>
            <relation name="UMR6249" active="#struct-441569" type="direct"></relation>
            <relation active="#struct-458810" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle active="#struct-426438" type="direct"><org type="regroupinstitution" xml:id="struct-426438" status="VALID">          <idno type="IdRef">200716271</idno>
          <orgName>Université Bourgogne Franche-Comté [COMUE]</orgName>
          <orgName type="acronym">UBFC</orgName>
          <date type="start">2015-04-01</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ubfc.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR6249" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-458810" type="direct"><org type="institution" xml:id="struct-458810" status="VALID">          <idno type="IdRef">026403188</idno>
          <idno type="ISNI">0000 0001 2188 3779 </idno>
          <orgName>Université de Franche-Comté</orgName>
          <orgName type="acronym">UFC</orgName>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-fcomte.fr</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city" wicri:auto="siege">Besançon</settlement>
<region type="region" nuts="2">Franche-Comté</region>
</placeName>
<orgName type="university">Université de Franche-Comté</orgName>
<orgName type="institution" wicri:auto="newGroup">Université de Bourgogne Franche-Comté</orgName>
</affiliation>
</author>
<author><name sortKey="Bertrand, X" sort="Bertrand, X" uniqKey="Bertrand X" first="X" last="Bertrand">X. Bertrand</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-57629" status="VALID">          <idno type="IdRef">166212377</idno>
          <idno type="RNSR">200812285Y</idno>
          <orgName>Laboratoire Chrono-environnement - UFC (UMR 6249)</orgName>
          <orgName type="acronym">LCE</orgName>
          <desc>            <address>              <addrLine>UFR Sciences et Techniques - 16, route de Gray - 25030 Besançon Cedex</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://chrono-environnement.univ-fcomte.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-426438" type="direct"></relation>
            <relation name="UMR6249" active="#struct-441569" type="direct"></relation>
            <relation active="#struct-458810" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle active="#struct-426438" type="direct"><org type="regroupinstitution" xml:id="struct-426438" status="VALID">          <idno type="IdRef">200716271</idno>
          <orgName>Université Bourgogne Franche-Comté [COMUE]</orgName>
          <orgName type="acronym">UBFC</orgName>
          <date type="start">2015-04-01</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ubfc.fr</ref>
          </desc>
        </org>
</tutelle>
<tutelle name="UMR6249" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-458810" type="direct"><org type="institution" xml:id="struct-458810" status="VALID">          <idno type="IdRef">026403188</idno>
          <idno type="ISNI">0000 0001 2188 3779 </idno>
          <orgName>Université de Franche-Comté</orgName>
          <orgName type="acronym">UFC</orgName>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-fcomte.fr</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city" wicri:auto="siege">Besançon</settlement>
<region type="region" nuts="2">Franche-Comté</region>
</placeName>
<orgName type="university">Université de Franche-Comté</orgName>
<orgName type="institution" wicri:auto="newGroup">Université de Bourgogne Franche-Comté</orgName>
</affiliation>
</author>
</analytic>
<series><title level="j">The Journal of hospital infection</title>
<idno type="ISSN">1532-2939</idno>
<imprint><date type="datePub">2015-06-30</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">              <p>Sequence type 131 (ST131) is a predominant lineage among extraintestinal pathogenic Escherichia coli. It plays a major role in the worldwide dissemination of extended-spectrum β-lactamase (ESBL)-producing E. coli. The ST131 pandemic is mainly the result of clonal expansion of the single well-adapted subclone H30-Rx, which is acquired in hospitals more frequently than other ESBL-producing E. coli clones. To develop a rapid method using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify ST131 for infection control purposes. Peak biomarkers of ST131 were identified from the mass spectrum profiles of 109 E. coli isolates (including 50 ST131 isolates). The models accurately identified ST131 isolates from mass spectrum profiles obtained with and without protein extraction. The rapid identification of ST131 isolates with MALDI-TOF MS can be easily implemented in the laboratory, and could help to target infection control measures in patients carrying multi-drug-resistant E. coli that are more likely to spread. </p>
            </div>
</front>
</TEI>
<hal api="V3">          <titleStmt>            <title xml:lang="en">Detection of Escherichia coli sequence type 131 by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: implications for infection control policies?</title>
            <author role="aut">              <persName>                <forename type="first">J</forename>
                <surname>Lafolie</surname>
              </persName>
              <idno type="halauthorid">1328722</idno>
            </author>
            <author role="aut">              <persName>                <forename type="first">M</forename>
                <surname>Sauget</surname>
              </persName>
              <idno type="halauthorid">1328723</idno>
              <affiliation ref="#struct-300165"></affiliation>
              <affiliation ref="#struct-57629"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">N</forename>
                <surname>Cabrolier</surname>
              </persName>
              <idno type="halauthorid">1328724</idno>
            </author>
            <author role="aut">              <persName>                <forename type="first">D</forename>
                <surname>Hocquet</surname>
              </persName>
              <idno type="halauthorid">1328686</idno>
              <affiliation ref="#struct-57629"></affiliation>
              <affiliation ref="#struct-300165"></affiliation>
            </author>
            <author role="aut">              <persName>                <forename type="first">X</forename>
                <surname>Bertrand</surname>
              </persName>
              <idno type="halauthorid">1126289</idno>
              <affiliation ref="#struct-57629"></affiliation>
              <affiliation ref="#struct-300165"></affiliation>
            </author>
            <editor role="depositor">              <persName>                <forename>Pascal</forename>
                <surname>Cholley</surname>
              </persName>
              <email type="md5">289fb269f92df7cf2b22284a6d51d91a</email>
              <email type="domain">chu-besancon.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>            <edition n="v1" type="current">              <date type="whenSubmitted">2016-04-22 11:43:08</date>
              <date type="whenModified">2018-09-05 17:04:03</date>
              <date type="whenReleased">2016-04-22 11:43:08</date>
              <date type="whenProduced">2015-06-30</date>
            </edition>
            <respStmt>              <resp>contributor</resp>
              <name key="134320">                <persName>                  <forename>Pascal</forename>
                  <surname>Cholley</surname>
                </persName>
                <email type="md5">289fb269f92df7cf2b22284a6d51d91a</email>
                <email type="domain">chu-besancon.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>            <distributor>CCSD</distributor>
            <idno type="halId">hal-01306067</idno>
            <idno type="halUri">https://hal.archives-ouvertes.fr/hal-01306067</idno>
            <idno type="halBibtex">lafolie:hal-01306067</idno>
            <idno type="halRefHtml">The Journal of hospital infection, 2015, 90 (3), pp.208-12</idno>
            <idno type="halRef">The Journal of hospital infection, 2015, 90 (3), pp.208-12</idno>
          </publicationStmt>
          <seriesStmt>            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="UNIV-FCOMTE">Université de Franche-Comté</idno>
            <idno type="stamp" n="CHRONO-ENVIRONNEMENT" corresp="UNIV-FCOMTE">Laboratoire Chrono Environnement</idno>
          </seriesStmt>
          <notesStmt>            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>            <biblStruct>              <analytic>                <title xml:lang="en">Detection of Escherichia coli sequence type 131 by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: implications for infection control policies?</title>
                <author role="aut">                  <persName>                    <forename type="first">J</forename>
                    <surname>Lafolie</surname>
                  </persName>
                  <idno type="halauthorid">1328722</idno>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">M</forename>
                    <surname>Sauget</surname>
                  </persName>
                  <idno type="halauthorid">1328723</idno>
                  <affiliation ref="#struct-300165"></affiliation>
                  <affiliation ref="#struct-57629"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">N</forename>
                    <surname>Cabrolier</surname>
                  </persName>
                  <idno type="halauthorid">1328724</idno>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">D</forename>
                    <surname>Hocquet</surname>
                  </persName>
                  <idno type="halauthorid">1328686</idno>
                  <affiliation ref="#struct-57629"></affiliation>
                  <affiliation ref="#struct-300165"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">X</forename>
                    <surname>Bertrand</surname>
                  </persName>
                  <idno type="halauthorid">1126289</idno>
                  <affiliation ref="#struct-57629"></affiliation>
                  <affiliation ref="#struct-300165"></affiliation>
                </author>
              </analytic>
              <monogr>                <idno type="halJournalId" status="INCOMING">108798</idno>
                <idno type="issn">1532-2939</idno>
                <title level="j">The Journal of hospital infection</title>
                <imprint>                  <biblScope unit="volume">90</biblScope>
                  <biblScope unit="issue">3</biblScope>
                  <biblScope unit="pp">208-12</biblScope>
                  <date type="datePub">2015-06-30</date>
                </imprint>
              </monogr>
              <idno type="pubmed">25799482</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>            <langUsage>              <language ident="en">English</language>
            </langUsage>
            <textClass>              <classCode scheme="mesh">Algorithms</classCode>
              <classCode scheme="mesh">Biomarkers</classCode>
              <classCode scheme="mesh">beta-Lactamases</classCode>
              <classCode scheme="mesh">Drug Resistance, Multiple, Bacterial</classCode>
              <classCode scheme="mesh">Escherichia coli</classCode>
              <classCode scheme="mesh">Escherichia coli Infections</classCode>
              <classCode scheme="mesh">Humans</classCode>
              <classCode scheme="mesh">Infection Control</classCode>
              <classCode scheme="mesh">Multilocus Sequence Typing</classCode>
              <classCode scheme="mesh">Sensitivity and Specificity</classCode>
              <classCode scheme="mesh">Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</classCode>
              <classCode scheme="halDomain" n="sdv.mp">Life Sciences [q-bio]/Microbiology and Parasitology</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">              <p>Sequence type 131 (ST131) is a predominant lineage among extraintestinal pathogenic Escherichia coli. It plays a major role in the worldwide dissemination of extended-spectrum β-lactamase (ESBL)-producing E. coli. The ST131 pandemic is mainly the result of clonal expansion of the single well-adapted subclone H30-Rx, which is acquired in hospitals more frequently than other ESBL-producing E. coli clones. To develop a rapid method using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify ST131 for infection control purposes. Peak biomarkers of ST131 were identified from the mass spectrum profiles of 109 E. coli isolates (including 50 ST131 isolates). The models accurately identified ST131 isolates from mass spectrum profiles obtained with and without protein extraction. The rapid identification of ST131 isolates with MALDI-TOF MS can be easily implemented in the laboratory, and could help to target infection control measures in patients carrying multi-drug-resistant E. coli that are more likely to spread. </p>
            </abstract>
          </profileDesc>
        </hal>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/PandemieGrippaleV1/Data/Hal/Checkpoint

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000375 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Hal/Checkpoint/biblio.hfd -nk 000375 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    PandemieGrippaleV1
   |flux=    Hal
   |étape=   Checkpoint
   |type=    RBID
   |clé=     Hal:hal-01306067
   |texte=   Detection of Escherichia coli sequence type 131 by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: implications for infection control policies?
}}

This area was generated with Dilib version V0.6.34.
Data generation: Wed Jun 10 11:04:28 2020. Site generation: Sun Mar 28 09:10:28 2021

	Serveur d'exploration sur les pandémies grippales
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur les pandémies grippales

Detection of Escherichia coli sequence type 131 by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: implications for infection control policies?

Detection of Escherichia coli sequence type 131 by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: implications for infection control policies?

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri