Optimization of influenza A vaccine virus by reverse genetic using chimeric HA and NA genes with an extended PR8 backbone.
Identifieur interne : 000387 ( France/Analysis ); précédent : 000386; suivant : 000388Optimization of influenza A vaccine virus by reverse genetic using chimeric HA and NA genes with an extended PR8 backbone.
Auteurs : Julie Medina [France] ; Houda Boukhebza [France] ; Amélie De Saint Jean [France] ; Régis Sodoyer [France] ; Isabelle Legastelois [France] ; Catherine Moste [France]Source :
- Vaccine [ 1873-2518 ] ; 2015.
Descripteurs français
- KwdFr :
- Alignement de séquences, Analyse de séquence d'ADN, Animaux, Antigènes viraux (génétique), Antigènes viraux (immunologie), Cellules Vero, Cellules rénales canines Madin-Darby, Chiens, Données de séquences moléculaires, Glycoprotéine hémagglutinine du virus influenza (), Glycoprotéine hémagglutinine du virus influenza (génétique), Glycoprotéine hémagglutinine du virus influenza (immunologie), Génétique inverse (), Humains, Protéines de fusion recombinantes (), Protéines de fusion recombinantes (immunologie), Sialidase (génétique), Sous-type H1N1 du virus de la grippe A (croissance et développement), Sous-type H1N1 du virus de la grippe A (génétique), Sous-type H1N1 du virus de la grippe A (immunologie), Séquence d'acides aminés, Vaccins antigrippaux (), Vaccins antigrippaux (génétique), Vaccins antigrippaux (immunologie), Virus recombinants (génétique).
- MESH :
- croissance et développement : Sous-type H1N1 du virus de la grippe A.
- génétique : Antigènes viraux, Glycoprotéine hémagglutinine du virus influenza, Sialidase, Sous-type H1N1 du virus de la grippe A, Vaccins antigrippaux, Virus recombinants.
- immunologie : Antigènes viraux, Glycoprotéine hémagglutinine du virus influenza, Protéines de fusion recombinantes, Sous-type H1N1 du virus de la grippe A, Vaccins antigrippaux.
- Alignement de séquences, Analyse de séquence d'ADN, Animaux, Cellules Vero, Cellules rénales canines Madin-Darby, Chiens, Données de séquences moléculaires, Glycoprotéine hémagglutinine du virus influenza, Génétique inverse, Humains, Protéines de fusion recombinantes, Séquence d'acides aminés, Vaccins antigrippaux.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antigens, Viral (genetics), Antigens, Viral (immunology), Chlorocebus aethiops, Dogs, Hemagglutinin Glycoproteins, Influenza Virus (chemistry), Hemagglutinin Glycoproteins, Influenza Virus (genetics), Hemagglutinin Glycoproteins, Influenza Virus (immunology), Humans, Influenza A Virus, H1N1 Subtype (genetics), Influenza A Virus, H1N1 Subtype (growth & development), Influenza A Virus, H1N1 Subtype (immunology), Influenza Vaccines (chemistry), Influenza Vaccines (genetics), Influenza Vaccines (immunology), Madin Darby Canine Kidney Cells, Molecular Sequence Data, Neuraminidase (genetics), Reassortant Viruses (genetics), Recombinant Fusion Proteins (chemistry), Recombinant Fusion Proteins (immunology), Reverse Genetics (methods), Sequence Alignment, Sequence Analysis, DNA, Vero Cells.
- MESH :
- chemical , chemistry : Hemagglutinin Glycoproteins, Influenza Virus, Influenza Vaccines, Recombinant Fusion Proteins.
- chemical , genetics : Antigens, Viral, Hemagglutinin Glycoproteins, Influenza Virus, Influenza Vaccines, Neuraminidase.
- chemical , immunology : Antigens, Viral, Hemagglutinin Glycoproteins, Influenza Virus, Influenza Vaccines, Recombinant Fusion Proteins.
- genetics : Influenza A Virus, H1N1 Subtype, Reassortant Viruses.
- growth & development : Influenza A Virus, H1N1 Subtype.
- immunology : Influenza A Virus, H1N1 Subtype.
- methods : Reverse Genetics.
- Amino Acid Sequence, Animals, Chlorocebus aethiops, Dogs, Humans, Madin Darby Canine Kidney Cells, Molecular Sequence Data, Sequence Alignment, Sequence Analysis, DNA, Vero Cells.
Abstract
The yield of influenza antigen production may significantly vary between vaccine strains; for example the A/California/07/09 (H1N1)-X179A vaccine virus, prepared during 2009 influenza pandemic, presented a low antigen yield in eggs compared to other seasonal H1N1 reassortants. In this study a bi-chimeric virus expressing HA and NA genes with A/Puerto Rico/8/34 (H1N1) (PR8) and X179A domains was rescued by reverse genetics using a mixture of Vero/CHOK1 cell lines (Medina et al. [7]). The bi-chimeric virus obtained demonstrated to yield much larger amounts of HA than X179A in eggs as measured by single-radial-immunodiffusion (SRID), the reference method to quantify HA protein in influenza vaccine. Such kind of optimized virus using PR8 backbone derived chimeric glycoproteins could be used as improved seed viruses for vaccine production.
DOI: 10.1016/j.vaccine.2015.06.112
PubMed: 26206270
Affiliations:
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pubmed:26206270Le document en format XML
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<term>Antigens, Viral (immunology)</term>
<term>Chlorocebus aethiops</term>
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<term>Molecular Sequence Data</term>
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<front><div type="abstract" xml:lang="en">The yield of influenza antigen production may significantly vary between vaccine strains; for example the A/California/07/09 (H1N1)-X179A vaccine virus, prepared during 2009 influenza pandemic, presented a low antigen yield in eggs compared to other seasonal H1N1 reassortants. In this study a bi-chimeric virus expressing HA and NA genes with A/Puerto Rico/8/34 (H1N1) (PR8) and X179A domains was rescued by reverse genetics using a mixture of Vero/CHOK1 cell lines (Medina et al. [7]). The bi-chimeric virus obtained demonstrated to yield much larger amounts of HA than X179A in eggs as measured by single-radial-immunodiffusion (SRID), the reference method to quantify HA protein in influenza vaccine. Such kind of optimized virus using PR8 backbone derived chimeric glycoproteins could be used as improved seed viruses for vaccine production. </div>
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<name sortKey="De Saint Jean, Amelie" sort="De Saint Jean, Amelie" uniqKey="De Saint Jean A" first="Amélie" last="De Saint Jean">Amélie De Saint Jean</name>
<name sortKey="Legastelois, Isabelle" sort="Legastelois, Isabelle" uniqKey="Legastelois I" first="Isabelle" last="Legastelois">Isabelle Legastelois</name>
<name sortKey="Moste, Catherine" sort="Moste, Catherine" uniqKey="Moste C" first="Catherine" last="Moste">Catherine Moste</name>
<name sortKey="Sodoyer, Regis" sort="Sodoyer, Regis" uniqKey="Sodoyer R" first="Régis" last="Sodoyer">Régis Sodoyer</name>
</country>
</tree>
</affiliations>
</record>
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