Plasmodium falciparum immunodetection in bone remains of members of the Renaissance Medici family (Florence, Italy, sixteenth century).
Identifieur interne : 000364 ( PubMed/Curation ); précédent : 000363; suivant : 000365Plasmodium falciparum immunodetection in bone remains of members of the Renaissance Medici family (Florence, Italy, sixteenth century).
Auteurs : Gino Fornaciari [Italie] ; Valentina Giuffra ; Ezio Ferroglio ; Sarah Gino ; Raffaella BianucciSource :
- Transactions of the Royal Society of Tropical Medicine and Hygiene [ 1878-3503 ] ; 2010.
Descripteurs français
- Wicri :
- geographic : Italie.
English descriptors
- KwdEn :
- Antigens, Protozoan (analysis), Archaeology, Bone and Bones (chemistry), Bone and Bones (parasitology), Cause of Death, Ceramics, Female, History, 16th Century, Humans, Italy, L-Lactate Dehydrogenase (analysis), Malaria, Falciparum (diagnosis), Malaria, Falciparum (history), Male, Plasmodium falciparum (chemistry), Plasmodium falciparum (isolation & purification), Protozoan Proteins (analysis).
- MESH :
- chemical , analysis : Antigens, Protozoan, L-Lactate Dehydrogenase, Protozoan Proteins.
- geographic : Italy.
- chemistry : Bone and Bones, Plasmodium falciparum.
- diagnosis : Malaria, Falciparum.
- history : Malaria, Falciparum.
- isolation & purification : Plasmodium falciparum.
- parasitology : Bone and Bones.
- Archaeology, Cause of Death, Ceramics, Female, History, 16th Century, Humans, Male.
Abstract
Medical accounts and ancient autopsy reports imply that tertian malarial fevers caused the death of four members of the Medici family of Florence: Eleonora of Toledo (1522-1562), Cardinal Giovanni (1543-1562), don Garzia (1547-1562) and Grand Duke Francesco I (1531-1587). All members of the Medici family hunted in the endemic malarial areas of Tuscany, such as the marshy areas surrounding their villas and along the swampy Maremma and were, therefore, highly exposed to the risk of being infected by Falciparum malaria. To determine if the original death certificates issued by the court physicians were correct, we carried out immunological investigations and then compared the biological results to the historical sources. Bone samples were examined for the presence of Plasmodium falciparum histidine-rich- protein-2 (PfHRP2) and P. falciparum lactate dehydrogenase (PfLDH) using two different qualitative double-antibody immunoassays. Our findings provide the first modern laboratory evidence of the presence of P. falciparum ancient proteins in the skeletal remains of four members of the Medici family. We confirm the clinical diagnosis of the court physicians, using modern methods. Finally, this study demonstrates that immunodetection can be successfully applied not only to mummified tissues but also to skeletal remains, thus opening new paths of investigation for large archaeological series.
DOI: 10.1016/j.trstmh.2010.06.007
PubMed: 20673935
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first="Valentina" last="Giuffra">Valentina Giuffra</name></author><author><name sortKey="Ferroglio, Ezio" sort="Ferroglio, Ezio" uniqKey="Ferroglio E" first="Ezio" last="Ferroglio">Ezio Ferroglio</name></author><author><name sortKey="Gino, Sarah" sort="Gino, Sarah" uniqKey="Gino S" first="Sarah" last="Gino">Sarah Gino</name></author><author><name sortKey="Bianucci, Raffaella" sort="Bianucci, Raffaella" uniqKey="Bianucci R" first="Raffaella" last="Bianucci">Raffaella Bianucci</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20673935</idno><idno type="pmid">20673935</idno><idno type="doi">10.1016/j.trstmh.2010.06.007</idno><idno type="wicri:Area/PubMed/Corpus">000364</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000364</idno><idno type="wicri:Area/PubMed/Curation">000364</idno><idno type="wicri:explorRef" wicri:stream="PubMed" 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qualifier="history" xml:lang="en"><term>Malaria, Falciparum</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Plasmodium falciparum</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Bone and Bones</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Archaeology</term><term>Cause of Death</term><term>Ceramics</term><term>Female</term><term>History, 16th Century</term><term>Humans</term><term>Male</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Italie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Medical accounts and ancient autopsy reports imply that tertian malarial fevers caused the death of four members of the Medici family of Florence: Eleonora of Toledo (1522-1562), Cardinal Giovanni (1543-1562), don Garzia (1547-1562) and Grand Duke Francesco I (1531-1587). All members of the Medici family hunted in the endemic malarial areas of Tuscany, such as the marshy areas surrounding their villas and along the swampy Maremma and were, therefore, highly exposed to the risk of being infected by Falciparum malaria. To determine if the original death certificates issued by the court physicians were correct, we carried out immunological investigations and then compared the biological results to the historical sources. Bone samples were examined for the presence of Plasmodium falciparum histidine-rich- protein-2 (PfHRP2) and P. falciparum lactate dehydrogenase (PfLDH) using two different qualitative double-antibody immunoassays. Our findings provide the first modern laboratory evidence of the presence of P. falciparum ancient proteins in the skeletal remains of four members of the Medici family. We confirm the clinical diagnosis of the court physicians, using modern methods. Finally, this study demonstrates that immunodetection can be successfully applied not only to mummified tissues but also to skeletal remains, thus opening new paths of investigation for large archaeological series.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20673935</PMID><DateCreated><Year>2010</Year><Month>08</Month><Day>16</Day></DateCreated><DateCompleted><Year>2011</Year><Month>02</Month><Day>24</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1878-3503</ISSN><JournalIssue CitedMedium="Internet"><Volume>104</Volume><Issue>9</Issue><PubDate><Year>2010</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Transactions of the Royal Society of Tropical Medicine and Hygiene</Title><ISOAbbreviation>Trans. R. Soc. Trop. Med. 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To determine if the original death certificates issued by the court physicians were correct, we carried out immunological investigations and then compared the biological results to the historical sources. Bone samples were examined for the presence of Plasmodium falciparum histidine-rich- protein-2 (PfHRP2) and P. falciparum lactate dehydrogenase (PfLDH) using two different qualitative double-antibody immunoassays. Our findings provide the first modern laboratory evidence of the presence of P. falciparum ancient proteins in the skeletal remains of four members of the Medici family. We confirm the clinical diagnosis of the court physicians, using modern methods. Finally, this study demonstrates that immunodetection can be successfully applied not only to mummified tissues but also to skeletal remains, thus opening new paths of investigation for large archaeological series.</AbstractText><CopyrightInformation>Copyright 2010 Royal Society of Tropical Medicine and Hygiene. 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