SL1 RNA gene recovery from Enterobius vermicularis ancient DNA in pre-Columbian human coprolites.
Identifieur interne : 000519 ( PubMed/Checkpoint ); précédent : 000518; suivant : 000520SL1 RNA gene recovery from Enterobius vermicularis ancient DNA in pre-Columbian human coprolites.
Auteurs : Alena Mayo I Iguez [Brésil] ; Karl Reinhard ; Marcelo Luiz Carvalho Gonçalves ; Luiz Fernando Ferreira ; Adauto Araújo ; Ana Carolina Paulo VicenteSource :
- International journal for parasitology [ 0020-7519 ] ; 2006.
English descriptors
- KwdEn :
- Animals, Base Sequence, DNA, Helminth (genetics), Enterobiasis (history), Enterobiasis (parasitology), Enterobius (classification), Enterobius (genetics), Enterobius (isolation & purification), History, Ancient, Humans, Molecular Sequence Data, Paleopathology (methods), Polymerase Chain Reaction (methods), RNA, Helminth (genetics), RNA, Spliced Leader (genetics), Sequence Analysis, DNA (methods).
- MESH :
- chemical , genetics : DNA, Helminth, RNA, Helminth, RNA, Spliced Leader.
- classification : Enterobius.
- genetics : Enterobius.
- history : Enterobiasis.
- isolation & purification : Enterobius.
- methods : Paleopathology, Polymerase Chain Reaction, Sequence Analysis, DNA.
- parasitology : Enterobiasis.
- Animals, Base Sequence, History, Ancient, Humans, Molecular Sequence Data.
Abstract
Enterobius vermicularis, pinworm, is one of the most common helminths worldwide, infecting nearly a billion people at all socio-economic levels. In prehistoric populations the paleoparasitological findings show a pinworm homogeneous distribution among hunter-gatherers in North America, intensified with the advent of agriculture. This same increase also occurred in the transition from nomad hunter-gatherers to sedentary farmers in South America, although E. vermicularis infection encompasses only the ancient Andean peoples, with no record among the pre-Colombian populations in the South American lowlands. However, the outline of pinworm paleoepidemiology has been supported by microscopic finding of eggs recovered from coprolites. Since molecular techniques are precise and sensitive in detecting pathogen ancient DNA (aDNA), and also could provide insights into the parasite evolutionary history, in this work we have performed a molecular paleoparasitological study of E. vermicularis. aDNA was recovered and pinworm 5S rRNA spacer sequences were determined from pre-Columbian coprolites (4110 BC-AD 900) from four different North and South American archaeological sites. The sequence analysis confirmed E. vermicularis identity and revealed a similarity among ancient and modern sequences. Moreover, polymorphisms were identified at the relative positions 160, 173 and 180, in independent coprolite samples from Tulán, San Pedro de Atacama, Chile (1080-950 BC). We also verified the presence of peculiarities (Splicing leader (SL1) RNA sequence, spliced donor site, the Sm antigen biding site, and RNA secondary structure) which characterise the SL1 RNA gene. The analysis shows that the SL1 RNA gene of contemporary pinworms was present in pre-Columbian E. vermicularis by 6110 years ago. We were successful in detecting E. vermicularis aDNA even in coprolites without direct microscopic evidence of the eggs, improving the diagnosis of helminth infections in the past and further pinworm paleoepidemiological studies.
DOI: 10.1016/j.ijpara.2006.07.005
PubMed: 16950265
Affiliations:
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Brasil 4365, Manguinhos 21045-900 Rio de Janeiro, RJ</wicri:regionArea><placeName><region type="state">État de Rio de Janeiro</region></placeName></affiliation></author><author><name sortKey="Reinhard, Karl" sort="Reinhard, Karl" uniqKey="Reinhard K" first="Karl" last="Reinhard">Karl Reinhard</name></author><author><name sortKey="Carvalho Goncalves, Marcelo Luiz" sort="Carvalho Goncalves, Marcelo Luiz" uniqKey="Carvalho Goncalves M" first="Marcelo Luiz" last="Carvalho Gonçalves">Marcelo Luiz Carvalho Gonçalves</name></author><author><name sortKey="Ferreira, Luiz Fernando" sort="Ferreira, Luiz Fernando" uniqKey="Ferreira L" first="Luiz Fernando" last="Ferreira">Luiz Fernando Ferreira</name></author><author><name sortKey="Araujo, Adauto" sort="Araujo, Adauto" uniqKey="Araujo A" first="Adauto" last="Araújo">Adauto Araújo</name></author><author><name sortKey="Paulo Vicente, Ana Carolina" sort="Paulo Vicente, Ana Carolina" uniqKey="Paulo Vicente A" first="Ana Carolina" last="Paulo Vicente">Ana Carolina Paulo Vicente</name></author></analytic><series><title level="j">International journal for parasitology</title><idno type="ISSN">0020-7519</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>DNA, Helminth (genetics)</term><term>Enterobiasis (history)</term><term>Enterobiasis (parasitology)</term><term>Enterobius (classification)</term><term>Enterobius (genetics)</term><term>Enterobius (isolation & purification)</term><term>History, Ancient</term><term>Humans</term><term>Molecular Sequence Data</term><term>Paleopathology (methods)</term><term>Polymerase Chain Reaction (methods)</term><term>RNA, Helminth (genetics)</term><term>RNA, Spliced Leader (genetics)</term><term>Sequence Analysis, DNA (methods)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Helminth</term><term>RNA, Helminth</term><term>RNA, Spliced Leader</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Enterobius</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Enterobius</term></keywords><keywords scheme="MESH" qualifier="history" xml:lang="en"><term>Enterobiasis</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Enterobius</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Paleopathology</term><term>Polymerase Chain Reaction</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Enterobiasis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>History, Ancient</term><term>Humans</term><term>Molecular Sequence Data</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Enterobius vermicularis, pinworm, is one of the most common helminths worldwide, infecting nearly a billion people at all socio-economic levels. In prehistoric populations the paleoparasitological findings show a pinworm homogeneous distribution among hunter-gatherers in North America, intensified with the advent of agriculture. This same increase also occurred in the transition from nomad hunter-gatherers to sedentary farmers in South America, although E. vermicularis infection encompasses only the ancient Andean peoples, with no record among the pre-Colombian populations in the South American lowlands. However, the outline of pinworm paleoepidemiology has been supported by microscopic finding of eggs recovered from coprolites. Since molecular techniques are precise and sensitive in detecting pathogen ancient DNA (aDNA), and also could provide insights into the parasite evolutionary history, in this work we have performed a molecular paleoparasitological study of E. vermicularis. aDNA was recovered and pinworm 5S rRNA spacer sequences were determined from pre-Columbian coprolites (4110 BC-AD 900) from four different North and South American archaeological sites. The sequence analysis confirmed E. vermicularis identity and revealed a similarity among ancient and modern sequences. Moreover, polymorphisms were identified at the relative positions 160, 173 and 180, in independent coprolite samples from Tulán, San Pedro de Atacama, Chile (1080-950 BC). We also verified the presence of peculiarities (Splicing leader (SL1) RNA sequence, spliced donor site, the Sm antigen biding site, and RNA secondary structure) which characterise the SL1 RNA gene. The analysis shows that the SL1 RNA gene of contemporary pinworms was present in pre-Columbian E. vermicularis by 6110 years ago. We were successful in detecting E. vermicularis aDNA even in coprolites without direct microscopic evidence of the eggs, improving the diagnosis of helminth infections in the past and further pinworm paleoepidemiological studies.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16950265</PMID><DateCreated><Year>2006</Year><Month>10</Month><Day>09</Day></DateCreated><DateCompleted><Year>2007</Year><Month>08</Month><Day>23</Day></DateCompleted><DateRevised><Year>2006</Year><Month>10</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0020-7519</ISSN><JournalIssue CitedMedium="Print"><Volume>36</Volume><Issue>13</Issue><PubDate><Year>2006</Year><Month>Nov</Month></PubDate></JournalIssue><Title>International journal for parasitology</Title><ISOAbbreviation>Int. J. 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Since molecular techniques are precise and sensitive in detecting pathogen ancient DNA (aDNA), and also could provide insights into the parasite evolutionary history, in this work we have performed a molecular paleoparasitological study of E. vermicularis. aDNA was recovered and pinworm 5S rRNA spacer sequences were determined from pre-Columbian coprolites (4110 BC-AD 900) from four different North and South American archaeological sites. The sequence analysis confirmed E. vermicularis identity and revealed a similarity among ancient and modern sequences. Moreover, polymorphisms were identified at the relative positions 160, 173 and 180, in independent coprolite samples from Tulán, San Pedro de Atacama, Chile (1080-950 BC). We also verified the presence of peculiarities (Splicing leader (SL1) RNA sequence, spliced donor site, the Sm antigen biding site, and RNA secondary structure) which characterise the SL1 RNA gene. The analysis shows that the SL1 RNA gene of contemporary pinworms was present in pre-Columbian E. vermicularis by 6110 years ago. We were successful in detecting E. vermicularis aDNA even in coprolites without direct microscopic evidence of the eggs, improving the diagnosis of helminth infections in the past and further pinworm paleoepidemiological studies.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Iñiguez</LastName><ForeName>Alena Mayo</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>Intituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. 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PubStatus="received"><Year>2006</Year><Month>05</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2006</Year><Month>07</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2006</Year><Month>07</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>9</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>8</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>9</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16950265</ArticleId><ArticleId IdType="pii">S0020-7519(06)00262-1</ArticleId><ArticleId IdType="doi">10.1016/j.ijpara.2006.07.005</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li></country><region><li>État de Rio de Janeiro</li></region></list><tree><noCountry><name sortKey="Araujo, Adauto" sort="Araujo, Adauto" uniqKey="Araujo A" first="Adauto" last="Araújo">Adauto Araújo</name><name sortKey="Carvalho Goncalves, Marcelo Luiz" sort="Carvalho Goncalves, Marcelo Luiz" uniqKey="Carvalho Goncalves M" first="Marcelo Luiz" last="Carvalho Gonçalves">Marcelo Luiz Carvalho Gonçalves</name><name sortKey="Ferreira, Luiz Fernando" sort="Ferreira, Luiz Fernando" uniqKey="Ferreira L" first="Luiz Fernando" last="Ferreira">Luiz Fernando Ferreira</name><name sortKey="Paulo Vicente, Ana Carolina" sort="Paulo Vicente, Ana Carolina" uniqKey="Paulo Vicente A" first="Ana Carolina" last="Paulo Vicente">Ana Carolina Paulo Vicente</name><name sortKey="Reinhard, Karl" sort="Reinhard, Karl" uniqKey="Reinhard K" first="Karl" last="Reinhard">Karl Reinhard</name></noCountry><country name="Brésil"><region name="État de Rio de Janeiro"><name sortKey="I Iguez, Alena Mayo" sort="I Iguez, Alena Mayo" uniqKey="I Iguez A" first="Alena Mayo" last="I Iguez">Alena Mayo I Iguez</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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