Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Colombian South America times.
Identifieur interne : 000454 ( PubMed/Checkpoint ); précédent : 000453; suivant : 000455Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Colombian South America times.
Auteurs : Daniela Leles [Brésil] ; Adauto Araújo ; Luiz Fernando Ferreira ; Ana Carolina Paulo Vicente ; Alena Mayo I IguezSource :
- Memorias do Instituto Oswaldo Cruz [ 0074-0276 ] ; 2008.
English descriptors
- KwdEn :
- Animals, Ascariasis (diagnosis), Ascariasis (history), Ascaris (genetics), Ascaris (isolation & purification), Cytochromes b (chemistry), Cytochromes b (genetics), DNA, Helminth (chemistry), DNA, Helminth (genetics), DNA, Helminth (isolation & purification), Feces (parasitology), History, Ancient, Humans, Paleopathology (methods), Polymerase Chain Reaction, Sensitivity and Specificity, Sequence Analysis, DNA, South America.
- MESH :
- chemical , chemistry : Cytochromes b, DNA, Helminth.
- geographic : South America.
- diagnosis : Ascariasis.
- genetics : Ascaris, Cytochromes b, DNA, Helminth.
- history : Ascariasis.
- isolation & purification : Ascaris, DNA, Helminth.
- methods : Paleopathology.
- parasitology : Feces.
- Animals, History, Ancient, Humans, Polymerase Chain Reaction, Sensitivity and Specificity, Sequence Analysis, DNA.
Abstract
Paleoparasitological studies using microscopy showed that Ascarisand Trichuris trichiura are the human intestinal parasites most found in archaeological sites. However, in pre-Columbian South American archaeological sites, Ascaris is rare. In this work we standardized a molecular methodology for Ascaris diagnosis directly from ancient DNA retrieved from coprolites. Using cythochrome b gene (142 bp) target, ancient DNA sequences were retrieved from South American samples, negative by microscopy. Moreover, the methodology applied was sensitive enough to detect ancient DNA extracted from 30 Ascaris eggs from an European coprolite. These results revealed a new scenery for the paleodistribution of Ascaris in South America.
PubMed: 18327505
Affiliations:
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Helminth</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Paleopathology</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Feces</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>History, Ancient</term><term>Humans</term><term>Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term><term>Sequence Analysis, DNA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Paleoparasitological studies using microscopy showed that Ascarisand Trichuris trichiura are the human intestinal parasites most found in archaeological sites. However, in pre-Columbian South American archaeological sites, Ascaris is rare. In this work we standardized a molecular methodology for Ascaris diagnosis directly from ancient DNA retrieved from coprolites. Using cythochrome b gene (142 bp) target, ancient DNA sequences were retrieved from South American samples, negative by microscopy. Moreover, the methodology applied was sensitive enough to detect ancient DNA extracted from 30 Ascaris eggs from an European coprolite. These results revealed a new scenery for the paleodistribution of Ascaris in South America.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18327505</PMID><DateCreated><Year>2008</Year><Month>03</Month><Day>27</Day></DateCreated><DateCompleted><Year>2008</Year><Month>10</Month><Day>14</Day></DateCompleted><DateRevised><Year>2008</Year><Month>03</Month><Day>27</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0074-0276</ISSN><JournalIssue CitedMedium="Print"><Volume>103</Volume><Issue>1</Issue><PubDate><Year>2008</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Memorias do Instituto Oswaldo Cruz</Title><ISOAbbreviation>Mem. Inst. 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America</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2007</Year><Month>09</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2007</Year><Month>12</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>3</Month><Day>11</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>10</Month><Day>15</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>3</Month><Day>11</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18327505</ArticleId><ArticleId IdType="pii">S0074-02762008005000004</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li></country></list><tree><noCountry><name sortKey="Araujo, Adauto" sort="Araujo, Adauto" uniqKey="Araujo A" first="Adauto" last="Araújo">Adauto Araújo</name><name sortKey="Ferreira, Luiz Fernando" sort="Ferreira, Luiz Fernando" uniqKey="Ferreira L" first="Luiz Fernando" last="Ferreira">Luiz Fernando Ferreira</name><name sortKey="I Iguez, Alena Mayo" sort="I Iguez, Alena Mayo" uniqKey="I Iguez A" first="Alena Mayo" last="I Iguez">Alena Mayo I Iguez</name><name sortKey="Vicente, Ana Carolina Paulo" sort="Vicente, Ana Carolina Paulo" uniqKey="Vicente A" first="Ana Carolina Paulo" last="Vicente">Ana Carolina Paulo Vicente</name></noCountry><country name="Brésil"><noRegion><name sortKey="Leles, Daniela" sort="Leles, Daniela" uniqKey="Leles D" first="Daniela" last="Leles">Daniela Leles</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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