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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Detection of Mycobacterial DNA in Andean Mummies</title><author><name sortKey="Konomi, Nami" sort="Konomi, Nami" uniqKey="Konomi N" first="Nami" last="Konomi">Nami Konomi</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Lebwohl, Eve" sort="Lebwohl, Eve" uniqKey="Lebwohl E" first="Eve" last="Lebwohl">Eve Lebwohl</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Mowbray, Ken" sort="Mowbray, Ken" uniqKey="Mowbray K" first="Ken" last="Mowbray">Ken Mowbray</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Tattersall, Ian" sort="Tattersall, Ian" uniqKey="Tattersall I" first="Ian" last="Tattersall">Ian Tattersall</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhang, David" sort="Zhang, David" uniqKey="Zhang D" first="David" last="Zhang">David Zhang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">12454182</idno><idno type="pmc">154635</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC154635</idno><idno type="RBID">PMC:154635</idno><idno type="doi">10.1128/JCM.40.12.4738-4740.2002</idno><date when="2002">2002</date><idno type="wicri:Area/Pmc/Corpus">000289</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000289</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Detection of Mycobacterial DNA in Andean Mummies</title><author><name sortKey="Konomi, Nami" sort="Konomi, Nami" uniqKey="Konomi N" first="Nami" last="Konomi">Nami Konomi</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Lebwohl, Eve" sort="Lebwohl, Eve" uniqKey="Lebwohl E" first="Eve" last="Lebwohl">Eve Lebwohl</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Mowbray, Ken" sort="Mowbray, Ken" uniqKey="Mowbray K" first="Ken" last="Mowbray">Ken Mowbray</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Tattersall, Ian" sort="Tattersall, Ian" uniqKey="Tattersall I" first="Ian" last="Tattersall">Ian Tattersall</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhang, David" sort="Zhang, David" uniqKey="Zhang D" first="David" last="Zhang">David Zhang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Clinical Microbiology</title><idno type="ISSN">0095-1137</idno><idno type="eISSN">1098-660X</idno><imprint><date when="2002">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The identification of genetic material from pathogenic organisms in ancient tissues provides a powerful tool for the study of certain infectious diseases in historic populations. We have obtained tissue samples from the genital areas of 12 mummies in the American Museum of Natural History collection in New York, N.Y. The mummies were excavated in the Andes Mountain region of South America, and radiocarbon dating estimates that the mummies date from <sc>a.d.</sc> 140 to 1200. DNAs were successfully extracted from all tissues and were suitable for PCR analysis. PCRs were carried out to detect <italic>Mycobacterium tuberculosis</italic> complex and mycobacteria other than <italic>M. tuberculosis</italic> (MOTB). <italic>M. tuberculosis</italic> complex was detected in 2 out of 12 samples, and MOTB were detected in 7 samples. This study confirmed the adequate preservation of genetic material in mummified tissues and the existence of mycobacteria, including <italic>M. tuberculosis</italic>, in historic populations in South America.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Microbiol</journal-id><journal-id journal-id-type="iso-abbrev">J. Clin. Microbiol</journal-id><journal-id journal-id-type="publisher-id">jcm</journal-id><journal-title-group><journal-title>Journal of Clinical Microbiology</journal-title></journal-title-group><issn pub-type="ppub">0095-1137</issn><issn pub-type="epub">1098-660X</issn><publisher><publisher-name>American Society for Microbiology (ASM)</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">12454182</article-id><article-id pub-id-type="pmc">154635</article-id><article-id pub-id-type="publisher-id">0880</article-id><article-id pub-id-type="doi">10.1128/JCM.40.12.4738-4740.2002</article-id><article-categories><subj-group subj-group-type="heading"><subject>Mycobacteriology and Aerobic Actinomycetes</subject></subj-group></article-categories><title-group><article-title>Detection of Mycobacterial DNA in Andean Mummies</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Konomi</surname><given-names>Nami</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Lebwohl</surname><given-names>Eve</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Mowbray</surname><given-names>Ken</given-names></name><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>Tattersall</surname><given-names>Ian</given-names></name><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>David</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff id="aff1">Department of Pathology, Mount Sinai School of Medicine, New York University,<label>1</label> American Museum of Natural History, New York, New York<label>2</label></aff><author-notes><corresp id="cor1"><label>*</label>Corresponding author. Mailing address: Molecular Pathology Laboratory, Mount Sinai School of Medicine, Box 1122, One Gustave Levy Pl., New York, NY 10021. Phone: (212) 659-8173. Fax: (212) 427-2082. E-mail: <email>David.Zhang@Msnyuhealth.org</email>.</corresp></author-notes><pub-date pub-type="ppub"><month>12</month><year>2002</year></pub-date><volume>40</volume><issue>12</issue><fpage>4738</fpage><lpage>4740</lpage><history><date date-type="received"><day>20</day><month>6</month><year>2002</year></date><date date-type="rev-recd"><day>18</day><month>7</month><year>2002</year></date><date date-type="accepted"><day>3</day><month>9</month><year>2002</year></date></history><permissions><copyright-statement>Copyright © 2002, American Society for Microbiology</copyright-statement><copyright-year>2002</copyright-year></permissions><abstract><p>The identification of genetic material from pathogenic organisms in ancient tissues provides a powerful tool for the study of certain infectious diseases in historic populations. We have obtained tissue samples from the genital areas of 12 mummies in the American Museum of Natural History collection in New York, N.Y. The mummies were excavated in the Andes Mountain region of South America, and radiocarbon dating estimates that the mummies date from <sc>a.d.</sc> 140 to 1200. DNAs were successfully extracted from all tissues and were suitable for PCR analysis. PCRs were carried out to detect <italic>Mycobacterium tuberculosis</italic> complex and mycobacteria other than <italic>M. tuberculosis</italic> (MOTB). <italic>M. tuberculosis</italic> complex was detected in 2 out of 12 samples, and MOTB were detected in 7 samples. This study confirmed the adequate preservation of genetic material in mummified tissues and the existence of mycobacteria, including <italic>M. tuberculosis</italic>, in historic populations in South America.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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