Amplification of DNA remnants in mummified human brains from medieval Joseon tombs of Korea.
Identifieur interne : 000C56 ( Ncbi/Merge ); précédent : 000C55; suivant : 000C57Amplification of DNA remnants in mummified human brains from medieval Joseon tombs of Korea.
Auteurs : Chang Seok Oh [Corée du Sud] ; Sang Jun Lee ; Soong Deok Lee ; Myeung Ju Kim ; Yi-Suk Kim ; Do-Seon Lim ; Dong Hoon ShinSource :
- Anthropologischer Anzeiger; Bericht uber die biologisch-anthropologische Literatur [ 0003-5548 ] ; 2013.
English descriptors
- KwdEn :
- Base Sequence, Brain (anatomy & histology), Brain (pathology), Brain Chemistry, DNA (analysis), DNA (genetics), DNA (isolation & purification), DNA, Mitochondrial (genetics), Female, Femur (chemistry), Humans, Male, Microsatellite Repeats, Middle Aged, Molecular Sequence Data, Mummies, Polymerase Chain Reaction, Republic of Korea, Young Adult.
- MESH :
- chemical , analysis : DNA.
- geographic : Republic of Korea.
- anatomy & histology : Brain.
- chemistry : Femur.
- chemical , genetics : DNA, DNA, Mitochondrial.
- chemical , isolation & purification : DNA.
- pathology : Brain.
- Base Sequence, Brain Chemistry, Female, Humans, Male, Microsatellite Repeats, Middle Aged, Molecular Sequence Data, Mummies, Polymerase Chain Reaction, Young Adult.
Abstract
Recently, a number of mummified brains obtained from the remains of medieval Joseon Koreans have been subjected to biological investigations. Although the morphology of the organs had been perfectly maintained on gross examination, we still do not know how well biomolecules such as DNA were preserved. In the present study, the preservation status of remnant DNA in mummified brain tissue was determined by means of comparisons with corresponding DNA taken from the femurs of the same subjects. Quantifiler analysis revealed that DNA from the mummified brain was less fragmented than that contained in the femurs. The better preservation status of the brain DNA was shown also in MiniFiler assays: the number of short tandem repeat (STR) locus profiles for the mummified brain was far higher than in the case of the femur bones. In the case of the mtDNA analysis, longer DNA fragments (821 bp) could be successfully amplified with brain samples, whereas only shorter PCR amplicons (221-263 bp) were seen with the femur samples. Indeed mummified brain tissue, if discovered in amounts suitable for ancient DNA analysis, promises to be the preferred source for genetic analysis of individuals from pre-modern Korean tombs.
PubMed: 23590113
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pubmed:23590113Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Amplification of DNA remnants in mummified human brains from medieval Joseon tombs of Korea.</title><author><name sortKey="Oh, Chang Seok" sort="Oh, Chang Seok" uniqKey="Oh C" first="Chang Seok" last="Oh">Chang Seok Oh</name><affiliation wicri:level="1"><nlm:affiliation>Anthropology and Paleopathology Lab, Department of Anatomy, Seoul National University College of Medicine, 28, Yongon-dong, Chongno-Gu, Seoul 110-799, Republic of Korea. drdoogi@snu.ac.kr</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Anthropology and Paleopathology Lab, Department of Anatomy, Seoul National University College of Medicine, 28, Yongon-dong, Chongno-Gu, Seoul 110-799</wicri:regionArea><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Lee, Sang Jun" sort="Lee, Sang Jun" uniqKey="Lee S" first="Sang Jun" last="Lee">Sang Jun Lee</name></author><author><name sortKey="Lee, Soong Deok" sort="Lee, Soong Deok" uniqKey="Lee S" first="Soong Deok" last="Lee">Soong Deok Lee</name></author><author><name sortKey="Kim, Myeung Ju" sort="Kim, Myeung Ju" uniqKey="Kim M" first="Myeung Ju" last="Kim">Myeung Ju Kim</name></author><author><name sortKey="Kim, Yi Suk" sort="Kim, Yi Suk" uniqKey="Kim Y" first="Yi-Suk" last="Kim">Yi-Suk Kim</name></author><author><name sortKey="Lim, Do Seon" sort="Lim, Do Seon" uniqKey="Lim D" first="Do-Seon" last="Lim">Do-Seon Lim</name></author><author><name sortKey="Shin, Dong Hoon" sort="Shin, Dong Hoon" uniqKey="Shin D" first="Dong Hoon" last="Shin">Dong Hoon Shin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23590113</idno><idno type="pmid">23590113</idno><idno type="wicri:Area/PubMed/Corpus">000244</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000244</idno><idno type="wicri:Area/PubMed/Curation">000244</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000244</idno><idno type="wicri:Area/PubMed/Checkpoint">000244</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000244</idno><idno type="wicri:Area/Ncbi/Merge">000C56</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Amplification of DNA remnants in mummified human brains from medieval Joseon tombs of Korea.</title><author><name sortKey="Oh, Chang Seok" sort="Oh, Chang Seok" uniqKey="Oh C" first="Chang Seok" last="Oh">Chang Seok Oh</name><affiliation wicri:level="1"><nlm:affiliation>Anthropology and Paleopathology Lab, Department of Anatomy, Seoul National University College of Medicine, 28, Yongon-dong, Chongno-Gu, Seoul 110-799, Republic of Korea. drdoogi@snu.ac.kr</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Anthropology and Paleopathology Lab, Department of Anatomy, Seoul National University College of Medicine, 28, Yongon-dong, Chongno-Gu, Seoul 110-799</wicri:regionArea><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Lee, Sang Jun" sort="Lee, Sang Jun" uniqKey="Lee S" first="Sang Jun" last="Lee">Sang Jun Lee</name></author><author><name sortKey="Lee, Soong Deok" sort="Lee, Soong Deok" uniqKey="Lee S" first="Soong Deok" last="Lee">Soong Deok Lee</name></author><author><name sortKey="Kim, Myeung Ju" sort="Kim, Myeung Ju" uniqKey="Kim M" first="Myeung Ju" last="Kim">Myeung Ju Kim</name></author><author><name sortKey="Kim, Yi Suk" sort="Kim, Yi Suk" uniqKey="Kim Y" first="Yi-Suk" last="Kim">Yi-Suk Kim</name></author><author><name sortKey="Lim, Do Seon" sort="Lim, Do Seon" uniqKey="Lim D" first="Do-Seon" last="Lim">Do-Seon Lim</name></author><author><name sortKey="Shin, Dong Hoon" sort="Shin, Dong Hoon" uniqKey="Shin D" first="Dong Hoon" last="Shin">Dong Hoon Shin</name></author></analytic><series><title level="j">Anthropologischer Anzeiger; Bericht uber die biologisch-anthropologische Literatur</title><idno type="ISSN">0003-5548</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Brain (anatomy & histology)</term><term>Brain (pathology)</term><term>Brain Chemistry</term><term>DNA (analysis)</term><term>DNA (genetics)</term><term>DNA (isolation & purification)</term><term>DNA, Mitochondrial (genetics)</term><term>Female</term><term>Femur (chemistry)</term><term>Humans</term><term>Male</term><term>Microsatellite Repeats</term><term>Middle Aged</term><term>Molecular Sequence Data</term><term>Mummies</term><term>Polymerase Chain Reaction</term><term>Republic of Korea</term><term>Young Adult</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Republic of Korea</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Brain</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Femur</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA</term><term>DNA, Mitochondrial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Brain</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Brain Chemistry</term><term>Female</term><term>Humans</term><term>Male</term><term>Microsatellite Repeats</term><term>Middle Aged</term><term>Molecular Sequence Data</term><term>Mummies</term><term>Polymerase Chain Reaction</term><term>Young Adult</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Recently, a number of mummified brains obtained from the remains of medieval Joseon Koreans have been subjected to biological investigations. Although the morphology of the organs had been perfectly maintained on gross examination, we still do not know how well biomolecules such as DNA were preserved. In the present study, the preservation status of remnant DNA in mummified brain tissue was determined by means of comparisons with corresponding DNA taken from the femurs of the same subjects. Quantifiler analysis revealed that DNA from the mummified brain was less fragmented than that contained in the femurs. The better preservation status of the brain DNA was shown also in MiniFiler assays: the number of short tandem repeat (STR) locus profiles for the mummified brain was far higher than in the case of the femur bones. In the case of the mtDNA analysis, longer DNA fragments (821 bp) could be successfully amplified with brain samples, whereas only shorter PCR amplicons (221-263 bp) were seen with the femur samples. Indeed mummified brain tissue, if discovered in amounts suitable for ancient DNA analysis, promises to be the preferred source for genetic analysis of individuals from pre-modern Korean tombs.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23590113</PMID><DateCreated><Year>2013</Year><Month>04</Month><Day>17</Day></DateCreated><DateCompleted><Year>2013</Year><Month>06</Month><Day>18</Day></DateCompleted><DateRevised><Year>2013</Year><Month>04</Month><Day>17</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0003-5548</ISSN><JournalIssue CitedMedium="Print"><Volume>70</Volume><Issue>1</Issue><PubDate><Year>2013</Year></PubDate></JournalIssue><Title>Anthropologischer Anzeiger; Bericht uber die biologisch-anthropologische Literatur</Title><ISOAbbreviation>Anthropol Anz</ISOAbbreviation></Journal><ArticleTitle>Amplification of DNA remnants in mummified human brains from medieval Joseon tombs of Korea.</ArticleTitle><Pagination><MedlinePgn>57-81</MedlinePgn></Pagination><Abstract><AbstractText>Recently, a number of mummified brains obtained from the remains of medieval Joseon Koreans have been subjected to biological investigations. Although the morphology of the organs had been perfectly maintained on gross examination, we still do not know how well biomolecules such as DNA were preserved. In the present study, the preservation status of remnant DNA in mummified brain tissue was determined by means of comparisons with corresponding DNA taken from the femurs of the same subjects. Quantifiler analysis revealed that DNA from the mummified brain was less fragmented than that contained in the femurs. The better preservation status of the brain DNA was shown also in MiniFiler assays: the number of short tandem repeat (STR) locus profiles for the mummified brain was far higher than in the case of the femur bones. In the case of the mtDNA analysis, longer DNA fragments (821 bp) could be successfully amplified with brain samples, whereas only shorter PCR amplicons (221-263 bp) were seen with the femur samples. Indeed mummified brain tissue, if discovered in amounts suitable for ancient DNA analysis, promises to be the preferred source for genetic analysis of individuals from pre-modern Korean tombs.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Oh</LastName><ForeName>Chang Seok</ForeName><Initials>CS</Initials><AffiliationInfo><Affiliation>Anthropology and Paleopathology Lab, Department of Anatomy, Seoul National University College of Medicine, 28, Yongon-dong, Chongno-Gu, Seoul 110-799, Republic of Korea. drdoogi@snu.ac.kr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Sang Jun</ForeName><Initials>SJ</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Soong Deok</ForeName><Initials>SD</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Myeung Ju</ForeName><Initials>MJ</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Yi-Suk</ForeName><Initials>YS</Initials></Author><Author ValidYN="Y"><LastName>Lim</LastName><ForeName>Do-Seon</ForeName><Initials>DS</Initials></Author><Author ValidYN="Y"><LastName>Shin</LastName><ForeName>Dong Hoon</ForeName><Initials>DH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Anthropol Anz</MedlineTA><NlmUniqueID>0372377</NlmUniqueID><ISSNLinking>0003-5548</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004272">DNA, Mitochondrial</NameOfSubstance></Chemical><Chemical><RegistryNumber>9007-49-2</RegistryNumber><NameOfSubstance UI="D004247">DNA</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001921" MajorTopicYN="N">Brain</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001923" MajorTopicYN="Y">Brain Chemistry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004247" MajorTopicYN="N">DNA</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004272" MajorTopicYN="N">DNA, Mitochondrial</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005269" MajorTopicYN="N">Femur</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018895" MajorTopicYN="N">Microsatellite Repeats</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009106" MajorTopicYN="Y">Mummies</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D056910" MajorTopicYN="N" Type="Geographic">Republic of Korea</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055815" MajorTopicYN="N">Young Adult</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>4</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>4</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>6</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23590113</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country><settlement><li>Séoul</li></settlement></list><tree><noCountry><name sortKey="Kim, Myeung Ju" sort="Kim, Myeung Ju" uniqKey="Kim M" first="Myeung Ju" last="Kim">Myeung Ju Kim</name><name sortKey="Kim, Yi Suk" sort="Kim, Yi Suk" uniqKey="Kim Y" first="Yi-Suk" last="Kim">Yi-Suk Kim</name><name sortKey="Lee, Sang Jun" sort="Lee, Sang Jun" uniqKey="Lee S" first="Sang Jun" last="Lee">Sang Jun Lee</name><name sortKey="Lee, Soong Deok" sort="Lee, Soong Deok" uniqKey="Lee S" first="Soong Deok" last="Lee">Soong Deok Lee</name><name sortKey="Lim, Do Seon" sort="Lim, Do Seon" uniqKey="Lim D" first="Do-Seon" last="Lim">Do-Seon Lim</name><name sortKey="Shin, Dong Hoon" sort="Shin, Dong Hoon" uniqKey="Shin D" first="Dong Hoon" last="Shin">Dong Hoon Shin</name></noCountry><country name="Corée du Sud"><noRegion><name sortKey="Oh, Chang Seok" sort="Oh, Chang Seok" uniqKey="Oh C" first="Chang Seok" last="Oh">Chang Seok Oh</name></noRegion></country></tree></affiliations></record>Pour 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