Multiplex analysis of genetic markers related to body mass index (BMI) and bone mineral density (BMD).
Identifieur interne : 000C41 ( Ncbi/Checkpoint ); précédent : 000C40; suivant : 000C42Multiplex analysis of genetic markers related to body mass index (BMI) and bone mineral density (BMD).
Auteurs : Carolin Fromm-Dornieden [Allemagne] ; Jutta Pepperl ; Bernd Herrmann ; Susanne HummelSource :
- Anthropologischer Anzeiger; Bericht uber die biologisch-anthropologische Literatur [ 0003-5548 ] ; 2012.
English descriptors
- KwdEn :
- Adult, Alpha-Ketoglutarate-Dependent Dioxygenase FTO, Aromatase (genetics), Base Sequence, Body Mass Index, Bone Density (genetics), Cemeteries, DNA, Female, Genetic Markers (genetics), Genotyping Techniques (methods), Germany, History, Medieval, Humans, Interleukin-6 (genetics), Low Density Lipoprotein Receptor-Related Protein-5 (genetics), Male, Microsatellite Repeats, Molecular Sequence Data, Paleopathology, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Proteins (genetics).
- MESH :
- chemical , genetics : Aromatase, Genetic Markers, Interleukin-6, Low Density Lipoprotein Receptor-Related Protein-5, Proteins.
- chemical : Alpha-Ketoglutarate-Dependent Dioxygenase FTO, DNA.
- genetics : Bone Density.
- methods : Genotyping Techniques.
- Adult, Base Sequence, Body Mass Index, Cemeteries, Female, Germany, History, Medieval, Humans, Male, Microsatellite Repeats, Molecular Sequence Data, Paleopathology, Polymerase Chain Reaction, Polymorphism, Single Nucleotide.
Abstract
The multiplex analysis system described here allows simultaneous typing of one short tandem repeat (STR) and three single nucleotide polymorphisms (SNPs) that are associated with obesity and/or osteoporosis. Genes that are related to a high body mass index (BMI) and/or a high bone mineral density (BMD) are presumed to give an advantage in surviving famines. This analysis system makes it possible to genotype the (TTTA)n polymorphism of CYP19 and three SNPs, namely the rs1800795 polymorphism of IL6, the rs373 6228 polymorphism of LRP5 and the rs993 9609 polymorphism of FTO, in a single PCR amplification in recent and ancient DNA samples. Furthermore, it allows a synchronous authentication of the results with the (TATC)n polymorphism of D13S317, the (TCTA)n polymorphism of D21S11 and the (TTTC)n polymorphism of FGA in a partial genetic fingerprinting. For this purpose, PCR products for fragment-length analysis, as well as those for sequence analysis, were amplified together. After amplification, the PCR product was split into two aliquots. The first aliquot was used for fragment-length analysis and the second one for sequence analysis. The analysis system described here has been optimized for analysing ancient samples, since only minimal amounts of material are available.
PubMed: 23350155
Affiliations:
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Bericht uber die biologisch-anthropologische Literatur</title><idno type="ISSN">0003-5548</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Alpha-Ketoglutarate-Dependent Dioxygenase FTO</term><term>Aromatase (genetics)</term><term>Base Sequence</term><term>Body Mass Index</term><term>Bone Density (genetics)</term><term>Cemeteries</term><term>DNA</term><term>Female</term><term>Genetic Markers (genetics)</term><term>Genotyping Techniques (methods)</term><term>Germany</term><term>History, Medieval</term><term>Humans</term><term>Interleukin-6 (genetics)</term><term>Low Density Lipoprotein Receptor-Related Protein-5 (genetics)</term><term>Male</term><term>Microsatellite Repeats</term><term>Molecular Sequence Data</term><term>Paleopathology</term><term>Polymerase Chain Reaction</term><term>Polymorphism, Single Nucleotide</term><term>Proteins (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Aromatase</term><term>Genetic Markers</term><term>Interleukin-6</term><term>Low Density Lipoprotein Receptor-Related Protein-5</term><term>Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Alpha-Ketoglutarate-Dependent Dioxygenase FTO</term><term>DNA</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bone Density</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Genotyping Techniques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Base Sequence</term><term>Body Mass Index</term><term>Cemeteries</term><term>Female</term><term>Germany</term><term>History, Medieval</term><term>Humans</term><term>Male</term><term>Microsatellite Repeats</term><term>Molecular Sequence Data</term><term>Paleopathology</term><term>Polymerase Chain Reaction</term><term>Polymorphism, Single Nucleotide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The multiplex analysis system described here allows simultaneous typing of one short tandem repeat (STR) and three single nucleotide polymorphisms (SNPs) that are associated with obesity and/or osteoporosis. Genes that are related to a high body mass index (BMI) and/or a high bone mineral density (BMD) are presumed to give an advantage in surviving famines. This analysis system makes it possible to genotype the (TTTA)n polymorphism of CYP19 and three SNPs, namely the rs1800795 polymorphism of IL6, the rs373 6228 polymorphism of LRP5 and the rs993 9609 polymorphism of FTO, in a single PCR amplification in recent and ancient DNA samples. Furthermore, it allows a synchronous authentication of the results with the (TATC)n polymorphism of D13S317, the (TCTA)n polymorphism of D21S11 and the (TTTC)n polymorphism of FGA in a partial genetic fingerprinting. For this purpose, PCR products for fragment-length analysis, as well as those for sequence analysis, were amplified together. After amplification, the PCR product was split into two aliquots. The first aliquot was used for fragment-length analysis and the second one for sequence analysis. The analysis system described here has been optimized for analysing ancient samples, since only minimal amounts of material are available.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country></list><tree><noCountry><name sortKey="Herrmann, Bernd" sort="Herrmann, Bernd" uniqKey="Herrmann B" first="Bernd" last="Herrmann">Bernd Herrmann</name><name sortKey="Hummel, Susanne" sort="Hummel, Susanne" uniqKey="Hummel S" first="Susanne" last="Hummel">Susanne Hummel</name><name sortKey="Pepperl, Jutta" sort="Pepperl, Jutta" uniqKey="Pepperl J" first="Jutta" last="Pepperl">Jutta Pepperl</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Fromm Dornieden, Carolin" sort="Fromm Dornieden, Carolin" uniqKey="Fromm Dornieden C" first="Carolin" last="Fromm-Dornieden">Carolin Fromm-Dornieden</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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