The 'common deletion' is not increased in parkinsonian substantia nigra as shown by competitive polymerase chain reaction.
Identifieur interne : 004613 ( PubMed/Corpus ); précédent : 004612; suivant : 004614The 'common deletion' is not increased in parkinsonian substantia nigra as shown by competitive polymerase chain reaction.
Auteurs : S. Kösel ; R. Egensperger ; N M Schnopp ; M B GraeberSource :
- Movement disorders : official journal of the Movement Disorder Society [ 0885-3185 ] ; 1997.
English descriptors
- KwdEn :
- Aged, Aged, 80 and over, Case-Control Studies, Cytochrome P-450 CYP2D6 (genetics), DNA, Mitochondrial (chemistry), DNA, Mitochondrial (genetics), Disease Susceptibility, Female, Gene Deletion, Genotype, Humans, Linear Models, Male, Middle Aged, NAD(P)H Dehydrogenase (Quinone) (deficiency), NAD(P)H Dehydrogenase (Quinone) (genetics), Parkinson Disease (genetics), Point Mutation, Polymerase Chain Reaction, Substantia Nigra (chemistry), Substantia Nigra (pathology).
- MESH :
- chemical , chemistry : DNA, Mitochondrial.
- chemical , deficiency : NAD(P)H Dehydrogenase (Quinone).
- chemical , genetics : Cytochrome P-450 CYP2D6, DNA, Mitochondrial, NAD(P)H Dehydrogenase (Quinone).
- chemistry : Substantia Nigra.
- genetics : Parkinson Disease.
- pathology : Substantia Nigra.
- Aged, Aged, 80 and over, Case-Control Studies, Disease Susceptibility, Female, Gene Deletion, Genotype, Humans, Linear Models, Male, Middle Aged, Point Mutation, Polymerase Chain Reaction.
Abstract
Previous studies have estimated levels of mitochondrial DNA (mtDNA) carrying the 4,977-base-pair 'common deletion' in tissues from patients with Parkinson's disease (PD) by using semiquantitative techniques. The role of this deleted mtDNA species in the pathogenesis of PD has remained controversial. We have applied competitive polymerase chain reaction to achieve exact quantitation of deleted mtDNA in the substantia nigra and additional brain regions of cases with neuropathologically confirmed Lewy-body parkinsonism. In addition, genotyping was carried out for CYP2D6G1,934A and CYP2D6C2,938T alleles and the mitochondrial ND2 (nucleotide 5,460) and transfer RNA for glutamine (nucleotide 4,336) sequence variants. Parkinsonian brains showed 1-3% deleted mtDNA in the substantia nigra, that is, deletion levels were not higher than in age-matched controls. Our findings suggest that the defect in complex I of the respiratory chain observed in PD is not primarily due to the 'common deletion.'
DOI: 10.1002/mds.870120504
PubMed: 9380043
Links to Exploration step
pubmed:9380043Le document en format XML
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Egensperger</name></author><author><name sortKey="Schnopp, N M" sort="Schnopp, N M" uniqKey="Schnopp N" first="N M" last="Schnopp">N M Schnopp</name></author><author><name sortKey="Graeber, M B" sort="Graeber, M B" uniqKey="Graeber M" first="M B" last="Graeber">M B Graeber</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1997">1997</date><idno type="RBID">pubmed:9380043</idno><idno type="pmid">9380043</idno><idno type="doi">10.1002/mds.870120504</idno><idno type="wicri:Area/PubMed/Corpus">004613</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The 'common deletion' is not increased in parkinsonian substantia nigra as shown by competitive polymerase chain reaction.</title><author><name sortKey="Kosel, S" sort="Kosel, S" uniqKey="Kosel S" first="S" last="Kösel">S. Kösel</name><affiliation><nlm:affiliation>Molecular Neuropathology Laboratory, Ludwig-Maximilians University, Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Egensperger, R" sort="Egensperger, R" uniqKey="Egensperger R" first="R" last="Egensperger">R. Egensperger</name></author><author><name sortKey="Schnopp, N M" sort="Schnopp, N M" uniqKey="Schnopp N" first="N M" last="Schnopp">N M Schnopp</name></author><author><name sortKey="Graeber, M B" sort="Graeber, M B" uniqKey="Graeber M" first="M B" last="Graeber">M B Graeber</name></author></analytic><series><title level="j">Movement disorders : official journal of the Movement Disorder Society</title><idno type="ISSN">0885-3185</idno><imprint><date when="1997" type="published">1997</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aged</term><term>Aged, 80 and over</term><term>Case-Control Studies</term><term>Cytochrome P-450 CYP2D6 (genetics)</term><term>DNA, Mitochondrial (chemistry)</term><term>DNA, Mitochondrial (genetics)</term><term>Disease Susceptibility</term><term>Female</term><term>Gene Deletion</term><term>Genotype</term><term>Humans</term><term>Linear Models</term><term>Male</term><term>Middle Aged</term><term>NAD(P)H Dehydrogenase (Quinone) (deficiency)</term><term>NAD(P)H Dehydrogenase (Quinone) (genetics)</term><term>Parkinson Disease (genetics)</term><term>Point Mutation</term><term>Polymerase Chain Reaction</term><term>Substantia Nigra (chemistry)</term><term>Substantia Nigra (pathology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Mitochondrial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="deficiency" xml:lang="en"><term>NAD(P)H Dehydrogenase (Quinone)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cytochrome P-450 CYP2D6</term><term>DNA, Mitochondrial</term><term>NAD(P)H Dehydrogenase (Quinone)</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Substantia Nigra</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Parkinson Disease</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Substantia Nigra</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Aged</term><term>Aged, 80 and over</term><term>Case-Control Studies</term><term>Disease Susceptibility</term><term>Female</term><term>Gene Deletion</term><term>Genotype</term><term>Humans</term><term>Linear Models</term><term>Male</term><term>Middle Aged</term><term>Point Mutation</term><term>Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Previous studies have estimated levels of mitochondrial DNA (mtDNA) carrying the 4,977-base-pair 'common deletion' in tissues from patients with Parkinson's disease (PD) by using semiquantitative techniques. The role of this deleted mtDNA species in the pathogenesis of PD has remained controversial. We have applied competitive polymerase chain reaction to achieve exact quantitation of deleted mtDNA in the substantia nigra and additional brain regions of cases with neuropathologically confirmed Lewy-body parkinsonism. In addition, genotyping was carried out for CYP2D6G1,934A and CYP2D6C2,938T alleles and the mitochondrial ND2 (nucleotide 5,460) and transfer RNA for glutamine (nucleotide 4,336) sequence variants. Parkinsonian brains showed 1-3% deleted mtDNA in the substantia nigra, that is, deletion levels were not higher than in age-matched controls. Our findings suggest that the defect in complex I of the respiratory chain observed in PD is not primarily due to the 'common deletion.'</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">9380043</PMID><DateCreated><Year>1997</Year><Month>11</Month><Day>07</Day></DateCreated><DateCompleted><Year>1997</Year><Month>11</Month><Day>07</Day></DateCompleted><DateRevised><Year>2007</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0885-3185</ISSN><JournalIssue CitedMedium="Print"><Volume>12</Volume><Issue>5</Issue><PubDate><Year>1997</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Movement disorders : official journal of the Movement Disorder Society</Title><ISOAbbreviation>Mov. Disord.</ISOAbbreviation></Journal><ArticleTitle>The 'common deletion' is not increased in parkinsonian substantia nigra as shown by competitive polymerase chain reaction.</ArticleTitle><Pagination><MedlinePgn>639-45</MedlinePgn></Pagination><Abstract><AbstractText>Previous studies have estimated levels of mitochondrial DNA (mtDNA) carrying the 4,977-base-pair 'common deletion' in tissues from patients with Parkinson's disease (PD) by using semiquantitative techniques. The role of this deleted mtDNA species in the pathogenesis of PD has remained controversial. We have applied competitive polymerase chain reaction to achieve exact quantitation of deleted mtDNA in the substantia nigra and additional brain regions of cases with neuropathologically confirmed Lewy-body parkinsonism. In addition, genotyping was carried out for CYP2D6G1,934A and CYP2D6C2,938T alleles and the mitochondrial ND2 (nucleotide 5,460) and transfer RNA for glutamine (nucleotide 4,336) sequence variants. Parkinsonian brains showed 1-3% deleted mtDNA in the substantia nigra, that is, deletion levels were not higher than in age-matched controls. Our findings suggest that the defect in complex I of the respiratory chain observed in PD is not primarily due to the 'common deletion.'</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kösel</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Molecular Neuropathology Laboratory, Ludwig-Maximilians University, Munich, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Egensperger</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Schnopp</LastName><ForeName>N M</ForeName><Initials>NM</Initials></Author><Author ValidYN="Y"><LastName>Graeber</LastName><ForeName>M B</ForeName><Initials>MB</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>UNITED STATES</Country><MedlineTA>Mov Disord</MedlineTA><NlmUniqueID>8610688</NlmUniqueID><ISSNLinking>0885-3185</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004272">DNA, Mitochondrial</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.14.14.1</RegistryNumber><NameOfSubstance UI="D019389">Cytochrome P-450 CYP2D6</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.6.5.2</RegistryNumber><NameOfSubstance UI="D016660">NAD(P)H Dehydrogenase (Quinone)</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" UI="D000368">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D000369">Aged, 80 and over</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D016022">Case-Control Studies</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D019389">Cytochrome P-450 CYP2D6</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000235">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D004272">DNA, Mitochondrial</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName><QualifierName MajorTopicYN="Y" UI="Q000235">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D004198">Disease Susceptibility</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D005260">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y" UI="D017353">Gene Deletion</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D005838">Genotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D006801">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D016014">Linear Models</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D008297">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D008875">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D016660">NAD(P)H Dehydrogenase (Quinone)</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000172">deficiency</QualifierName><QualifierName MajorTopicYN="N" UI="Q000235">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D010300">Parkinson Disease</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000235">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D017354">Point Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D016133">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D013378">Substantia Nigra</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000737">chemistry</QualifierName><QualifierName MajorTopicYN="N" UI="Q000473">pathology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1997</Year><Month>9</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1997</Year><Month>9</Month><Day>26</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1997</Year><Month>9</Month><Day>26</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9380043</ArticleId><ArticleId IdType="doi">10.1002/mds.870120504</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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