Detection of antibodies against botulinum toxins
Identifieur interne : 002186 ( PascalFrancis/Checkpoint ); précédent : 002185; suivant : 002187Detection of antibodies against botulinum toxins
Auteurs : Dorothea Sesardic [Royaume-Uni] ; Russell G. A. Jones [Royaume-Uni] ; Tong Leung [Royaume-Uni] ; Toni Alsop [Royaume-Uni] ; Robert Tierney [Royaume-Uni]Source :
- Movement disorders [ 0885-3185 ] ; 2004.
Descripteurs français
- Pascal (Inist)
English descriptors
Abstract
After immunisation with botulinum vaccine, antibodies to multiple epitopes are produced. Only some of these will have the capacity to neutralise the toxin activity. In fact, the ability of toxoid vaccine to induce toxin neutralising antibodies has provided the basis for the use of therapeutic antitoxins and immunoglobulins for the prophylaxis and treatment of diseases caused by bacterial toxins. Increasing indications for the chronic use of botulinum toxin for therapy have inevitably resulted in concern for patients becoming unresponsive because of the presence of circulating toxin-specific antibodies. Highly sensitive and relevant assays to detect only clinically relevant toxin neutralising antibodies are essential. Although immunoassays often provide the sensitivity, their relevance and specificity is often questioned. The mouse protection LD50 bioassay is considered most relevant but can often only detect 10 mIU/ml of antitoxin. This sensitivity, although sufficient for confirming protective immunity, is inadequate for patients undergoing toxin therapy. An intramuscular paralysis assay improves the sensitivity to ca. 1 mIU/ml, and a mouse ex vivo diaphragm assay, with sensitivity of <0.5 mIU/ml, is the most sensitive functional assay to date for this purpose. Alternative approaches for the detection of antibodies to botulinum toxin have included in vitro endopeptidase activity neutralisation. Unlike any other functional assay, this approach is not reliant on serotype-specific antibodies for specificity. Most recent promising developments are focused on cellular assays utilising primary rat embryonic cord cells or more conveniently in vitro differentiated established cell lines such as human neuroblastoma cells.
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A." last="Jones">Russell G. A. Jones</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Division of Bacteriology, National Institute for Biological Standards and Control</s1><s2>Potters Bar, Hertfordshire</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>Potters Bar, Hertfordshire</wicri:noRegion></affiliation></author><author><name sortKey="Leung, Tong" sort="Leung, Tong" uniqKey="Leung T" first="Tong" last="Leung">Tong Leung</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Division of Bacteriology, National Institute for Biological Standards and Control</s1><s2>Potters Bar, Hertfordshire</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>Potters Bar, Hertfordshire</wicri:noRegion></affiliation></author><author><name sortKey="Alsop, Toni" sort="Alsop, Toni" uniqKey="Alsop T" first="Toni" last="Alsop">Toni Alsop</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Division of Bacteriology, National Institute for Biological Standards and Control</s1><s2>Potters Bar, Hertfordshire</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>Potters Bar, Hertfordshire</wicri:noRegion></affiliation></author><author><name sortKey="Tierney, Robert" sort="Tierney, Robert" uniqKey="Tierney R" first="Robert" last="Tierney">Robert Tierney</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Division of Bacteriology, National Institute for Biological Standards and Control</s1><s2>Potters Bar, Hertfordshire</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>Potters Bar, Hertfordshire</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Movement disorders</title><title level="j" type="abbreviated">Mov. disord.</title><idno type="ISSN">0885-3185</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Movement disorders</title><title level="j" type="abbreviated">Mov. disord.</title><idno type="ISSN">0885-3185</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bontoxilysin</term><term>Medical screening</term><term>Nervous system diseases</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Système nerveux pathologie</term><term>Dépistage</term><term>Bontoxilysin</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">After immunisation with botulinum vaccine, antibodies to multiple epitopes are produced. Only some of these will have the capacity to neutralise the toxin activity. In fact, the ability of toxoid vaccine to induce toxin neutralising antibodies has provided the basis for the use of therapeutic antitoxins and immunoglobulins for the prophylaxis and treatment of diseases caused by bacterial toxins. Increasing indications for the chronic use of botulinum toxin for therapy have inevitably resulted in concern for patients becoming unresponsive because of the presence of circulating toxin-specific antibodies. Highly sensitive and relevant assays to detect only clinically relevant toxin neutralising antibodies are essential. Although immunoassays often provide the sensitivity, their relevance and specificity is often questioned. The mouse protection LD<sub>50</sub> bioassay is considered most relevant but can often only detect 10 mIU/ml of antitoxin. This sensitivity, although sufficient for confirming protective immunity, is inadequate for patients undergoing toxin therapy. An intramuscular paralysis assay improves the sensitivity to ca. 1 mIU/ml, and a mouse ex vivo diaphragm assay, with sensitivity of <0.5 mIU/ml, is the most sensitive functional assay to date for this purpose. Alternative approaches for the detection of antibodies to botulinum toxin have included in vitro endopeptidase activity neutralisation. Unlike any other functional assay, this approach is not reliant on serotype-specific antibodies for specificity. Most recent promising developments are focused on cellular assays utilising primary rat embryonic cord cells or more conveniently in vitro differentiated established cell lines such as human neuroblastoma cells.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0885-3185</s0></fA01><fA03 i2="1"><s0>Mov. disord.</s0></fA03><fA05><s2>19</s2></fA05><fA06><s3>SUP8</s3></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Detection of antibodies against botulinum toxins</s1></fA08><fA09 i1="01" i2="1" l="ENG"><s1>Basic and Therapeutic Aspects of Neurotoxins</s1></fA09><fA11 i1="01" i2="1"><s1>SESARDIC (Dorothea)</s1></fA11><fA11 i1="02" i2="1"><s1>JONES (Russell G. A.)</s1></fA11><fA11 i1="03" i2="1"><s1>LEUNG (Tong)</s1></fA11><fA11 i1="04" i2="1"><s1>ALSOP (Toni)</s1></fA11><fA11 i1="05" i2="1"><s1>TIERNEY (Robert)</s1></fA11><fA12 i1="01" i2="1"><s1>BIGALKE (HANS)</s1><s9>ed.</s9></fA12><fA12 i1="02" i2="1"><s1>DRESSLER (Dirk)</s1><s9>ed.</s9></fA12><fA12 i1="03" i2="1"><s1>JANKOVIC (Joseph)</s1><s9>ed.</s9></fA12><fA14 i1="01"><s1>Division of Bacteriology, National Institute for Biological Standards and Control</s1><s2>Potters Bar, Hertfordshire</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></fA14><fA15 i1="01"><s1>Institute of Toxicology, Medical School of Hannover</s1><s2>Hannover</s2><s3>DEU</s3><sZ>1 aut.</sZ></fA15><fA15 i1="02"><s1>Department of Neurology, Rostock University</s1><s2>Rostock</s2><s3>DEU</s3><sZ>2 aut.</sZ></fA15><fA15 i1="03"><s1>Parkinson's Disease Center and Movement Disorders Clinic, Department of Neurology, Baylor College of Medicine</s1><s2>Houston, Texas</s2><s3>USA</s3><sZ>3 aut.</sZ></fA15><fA20><s1>85-91</s1></fA20><fA21><s1>2004</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>20953</s2><s5>354000113591720120</s5></fA43><fA44><s0>0000</s0><s1>© 2004 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>66 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>04-0228587</s0></fA47><fA60><s1>P</s1><s2>C</s2></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Movement disorders</s0></fA64><fA66 i1="01"><s0>USA</s0></fA66><fC01 i1="01" l="ENG"><s0>After immunisation with botulinum vaccine, antibodies to multiple epitopes are produced. Only some of these will have the capacity to neutralise the toxin activity. In fact, the ability of toxoid vaccine to induce toxin neutralising antibodies has provided the basis for the use of therapeutic antitoxins and immunoglobulins for the prophylaxis and treatment of diseases caused by bacterial toxins. Increasing indications for the chronic use of botulinum toxin for therapy have inevitably resulted in concern for patients becoming unresponsive because of the presence of circulating toxin-specific antibodies. Highly sensitive and relevant assays to detect only clinically relevant toxin neutralising antibodies are essential. Although immunoassays often provide the sensitivity, their relevance and specificity is often questioned. The mouse protection LD<sub>50</sub> bioassay is considered most relevant but can often only detect 10 mIU/ml of antitoxin. This sensitivity, although sufficient for confirming protective immunity, is inadequate for patients undergoing toxin therapy. An intramuscular paralysis assay improves the sensitivity to ca. 1 mIU/ml, and a mouse ex vivo diaphragm assay, with sensitivity of <0.5 mIU/ml, is the most sensitive functional assay to date for this purpose. Alternative approaches for the detection of antibodies to botulinum toxin have included in vitro endopeptidase activity neutralisation. Unlike any other functional assay, this approach is not reliant on serotype-specific antibodies for specificity. 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Conference</s1><s3>Hannover DEU</s3><s4>2002</s4></fA30></pR></standard></inist><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Sesardic, Dorothea" sort="Sesardic, Dorothea" uniqKey="Sesardic D" first="Dorothea" last="Sesardic">Dorothea Sesardic</name></noRegion><name sortKey="Alsop, Toni" sort="Alsop, Toni" uniqKey="Alsop T" first="Toni" last="Alsop">Toni Alsop</name><name sortKey="Jones, Russell G A" sort="Jones, Russell G A" uniqKey="Jones R" first="Russell G. A." last="Jones">Russell G. A. Jones</name><name sortKey="Leung, Tong" sort="Leung, Tong" uniqKey="Leung T" first="Tong" last="Leung">Tong Leung</name><name sortKey="Tierney, Robert" sort="Tierney, Robert" uniqKey="Tierney R" first="Robert" last="Tierney">Robert Tierney</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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