DNA fragmentation in human substantia nigra: apoptosis or perimortem effect?
Identifieur interne : 005067 ( Ncbi/Checkpoint ); précédent : 005066; suivant : 005068DNA fragmentation in human substantia nigra: apoptosis or perimortem effect?
Auteurs : A E Kingsbury [Royaume-Uni] ; C D Mardsen ; O J FosterSource :
- Movement disorders : official journal of the Movement Disorder Society [ 0885-3185 ] ; 1998.
English descriptors
- KwdEn :
- MESH :
Abstract
DNA fragmentation was examined in situ in flash-frozen human postmortem midbrain as a marker for programmed cell death. A large series of cases comprising 16 pathologically confirmed idiopathic Parkinson's disease (IPD) cases, 14 control cases without brain pathology, and a group of 6 patients with other parkinsonian movement disorders were examined using TdT-mediated dUTP-biotin 3' end-labeling histology. Labeling of neurons and glia was seen in the substantia nigra of control and IPD cases and in other movement disorder cases. Labeled nuclei were seen in melanized nigral neurons; apoptotic bodies were also found but were more commonly associated with nigral glia. In the control group, labeling of neurons and glia was strongly associated with poor agonal status, assessed by tissue pH, a marker for antemortem hypoxia. The mean tissue pH of the control group with neuronal labeling was 6.28 (SEM .057), which was significantly different from that of the unlabeled group 6.55 (SEM .055). Mean tissue pH for all cases was 6.38. There was no association of nigral neuronal labeling with poor agonal status in the IPD cases, which showed labeling throughout the range of pH values. However, extranigral labeling, seen in the mesencephalon, red nucleus, superior colliculus, rostral pons, and periaqueductal gray matter, in all three subject groups was associated with tissue pH values of less than 6.3. These findings suggest that DNA fragmentation is influenced by antemortem hypoxia and that apoptosis-like changes seen in the postmortem nigra may parallel those seen in experimental ischemia in the animal brain. The likely influence of perimortem factors on these changes indicates that results from postmortem studies of apoptotic cell death in neurodegenerative disease should be treated with caution and underlines the importance of determining postmortem markers for agonal status in human brain.
DOI: 10.1002/mds.870130604
PubMed: 9827610
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 004316
- to stream PubMed, to step Curation: 004316
- to stream PubMed, to step Checkpoint: 004465
- to stream Ncbi, to step Merge: 005067
- to stream Ncbi, to step Curation: 005067
Links to Exploration step
pubmed:9827610Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">DNA fragmentation in human substantia nigra: apoptosis or perimortem effect?</title><author><name sortKey="Kingsbury, A E" sort="Kingsbury, A E" uniqKey="Kingsbury A" first="A E" last="Kingsbury">A E Kingsbury</name><affiliation wicri:level="3"><nlm:affiliation>Parkinson's Disease Society Brain Research Centre, Institute of Neurology, London, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Parkinson's Disease Society Brain Research Centre, Institute of Neurology, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Mardsen, C D" sort="Mardsen, C D" uniqKey="Mardsen C" first="C D" last="Mardsen">C D Mardsen</name></author><author><name sortKey="Foster, O J" sort="Foster, O J" uniqKey="Foster O" first="O J" last="Foster">O J Foster</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1998">1998</date><idno type="RBID">pubmed:9827610</idno><idno type="pmid">9827610</idno><idno type="doi">10.1002/mds.870130604</idno><idno type="wicri:Area/PubMed/Corpus">004316</idno><idno type="wicri:Area/PubMed/Curation">004316</idno><idno type="wicri:Area/PubMed/Checkpoint">004465</idno><idno type="wicri:Area/Ncbi/Merge">005067</idno><idno type="wicri:Area/Ncbi/Curation">005067</idno><idno type="wicri:Area/Ncbi/Checkpoint">005067</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">DNA fragmentation in human substantia nigra: apoptosis or perimortem effect?</title><author><name sortKey="Kingsbury, A E" sort="Kingsbury, A E" uniqKey="Kingsbury A" first="A E" last="Kingsbury">A E Kingsbury</name><affiliation wicri:level="3"><nlm:affiliation>Parkinson's Disease Society Brain Research Centre, Institute of Neurology, London, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Parkinson's Disease Society Brain Research Centre, Institute of Neurology, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Mardsen, C D" sort="Mardsen, C D" uniqKey="Mardsen C" first="C D" last="Mardsen">C D Mardsen</name></author><author><name sortKey="Foster, O J" sort="Foster, O J" uniqKey="Foster O" first="O J" last="Foster">O J Foster</name></author></analytic><series><title level="j">Movement disorders : official journal of the Movement Disorder Society</title><idno type="ISSN">0885-3185</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aged</term><term>Aged, 80 and over</term><term>Apoptosis</term><term>Cadaver</term><term>DNA Fragmentation</term><term>Female</term><term>Humans</term><term>Hydrogen-Ion Concentration</term><term>In Situ Nick-End Labeling</term><term>Male</term><term>Movement Disorders (pathology)</term><term>Parkinson Disease (pathology)</term><term>Substantia Nigra (pathology)</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Movement Disorders</term><term>Parkinson Disease</term><term>Substantia Nigra</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Aged</term><term>Aged, 80 and over</term><term>Apoptosis</term><term>Cadaver</term><term>DNA Fragmentation</term><term>Female</term><term>Humans</term><term>Hydrogen-Ion Concentration</term><term>In Situ Nick-End Labeling</term><term>Male</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">DNA fragmentation was examined in situ in flash-frozen human postmortem midbrain as a marker for programmed cell death. A large series of cases comprising 16 pathologically confirmed idiopathic Parkinson's disease (IPD) cases, 14 control cases without brain pathology, and a group of 6 patients with other parkinsonian movement disorders were examined using TdT-mediated dUTP-biotin 3' end-labeling histology. Labeling of neurons and glia was seen in the substantia nigra of control and IPD cases and in other movement disorder cases. Labeled nuclei were seen in melanized nigral neurons; apoptotic bodies were also found but were more commonly associated with nigral glia. In the control group, labeling of neurons and glia was strongly associated with poor agonal status, assessed by tissue pH, a marker for antemortem hypoxia. The mean tissue pH of the control group with neuronal labeling was 6.28 (SEM .057), which was significantly different from that of the unlabeled group 6.55 (SEM .055). Mean tissue pH for all cases was 6.38. There was no association of nigral neuronal labeling with poor agonal status in the IPD cases, which showed labeling throughout the range of pH values. However, extranigral labeling, seen in the mesencephalon, red nucleus, superior colliculus, rostral pons, and periaqueductal gray matter, in all three subject groups was associated with tissue pH values of less than 6.3. These findings suggest that DNA fragmentation is influenced by antemortem hypoxia and that apoptosis-like changes seen in the postmortem nigra may parallel those seen in experimental ischemia in the animal brain. The likely influence of perimortem factors on these changes indicates that results from postmortem studies of apoptotic cell death in neurodegenerative disease should be treated with caution and underlines the importance of determining postmortem markers for agonal status in human brain.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Grand Londres</li></region><settlement><li>Londres</li></settlement></list><tree><noCountry><name sortKey="Foster, O J" sort="Foster, O J" uniqKey="Foster O" first="O J" last="Foster">O J Foster</name><name sortKey="Mardsen, C D" sort="Mardsen, C D" uniqKey="Mardsen C" first="C D" last="Mardsen">C D Mardsen</name></noCountry><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Kingsbury, A E" sort="Kingsbury, A E" uniqKey="Kingsbury A" first="A E" last="Kingsbury">A E Kingsbury</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Santé/explor/MovDisordV3/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005067 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 005067 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Santé
|area= MovDisordV3
|flux= Ncbi
|étape= Checkpoint
|type= RBID
|clé= pubmed:9827610
|texte= DNA fragmentation in human substantia nigra: apoptosis or perimortem effect?
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:9827610" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a MovDisordV3
| This area was generated with Dilib version V0.6.23. |  |