Anti-basal ganglia antibodies in PANDAS.
Identifieur interne : 000D93 ( Ncbi/Checkpoint ); précédent : 000D92; suivant : 000D94Anti-basal ganglia antibodies in PANDAS.
Auteurs : Harvey S. Singer [États-Unis] ; Christopher R. Loiselle ; Olivia Lee ; Karen Minzer ; Susan Swedo ; Franz H. GrusSource :
- Movement disorders : official journal of the Movement Disorder Society [ 0885-3185 ] ; 2004.
English descriptors
- KwdEn :
- Adolescent, Antibodies, Anti-Idiotypic (immunology), Autoimmune Diseases (immunology), Autoimmune Diseases (microbiology), Basal Ganglia (immunology), Basal Ganglia (pathology), Blotting, Western, Brain (immunology), Brain (microbiology), Child, Child, Preschool, Enzyme-Linked Immunosorbent Assay, Female, Globus Pallidus (immunology), Globus Pallidus (pathology), Humans, Male, Mental Disorders (microbiology), Streptococcal Infections (complications), Streptococcal Infections (immunology), Tics (immunology), Tics (microbiology).
- MESH :
- chemical , immunology : Antibodies, Anti-Idiotypic.
- complications : Streptococcal Infections.
- immunology : Autoimmune Diseases, Basal Ganglia, Brain, Globus Pallidus, Streptococcal Infections, Tics.
- microbiology : Autoimmune Diseases, Brain, Mental Disorders, Tics.
- pathology : Basal Ganglia, Globus Pallidus.
- Adolescent, Blotting, Western, Child, Child, Preschool, Enzyme-Linked Immunosorbent Assay, Female, Humans, Male.
Abstract
An autoimmune-mediated mechanism involving molecular mimicry has been proposed for a variety of pediatric movement disorders that occur after a streptococcal infection. In this study, anti-basal ganglia antibodies (ABGA) were measured in 15 children with the diagnosis of pediatric autoimmune neuropsychiatric disorder associated with streptococcal infection (PANDAS) and compared with those in 15 controls. ELISA and Western immunoblotting (WB) methods were used to detect ABGA against supernatant (S1), pellet (P2), and synaptosomal preparations from adult postmortem caudate, putamen, and globus pallidus. ELISA optical density values did not differ between PANDAS patients and controls across all preparations. Immunoblotting identified multiple bands in all subjects with no differences in the number of bands or their total density. Discriminant analysis, used to assess mean binding patterns, showed that PANDAS patients differed from controls only for the caudate S1 fraction (Wilks' lambda = 0.0236, P < 0.0002), with PANDAS-primarily tic subjects providing the greatest discrimination. Among the epitopes contributing to differences between PANDAS and control in the caudate S1 fraction, mean binding to the epitope at 183 kDa was the most different between groups. In conclusion, ELISA measurements do not differentiate between PANDAS and controls, suggesting a lack of major antibody changes in this disorder. Further immunoblot analyses using a caudate supernatant fraction are required to completely exclude the possibility of minor antibody repertoire differences in PANDAS subjects, especially in those who primarily have tics.
DOI: 10.1002/mds.20052
PubMed: 15077238
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Anti-basal ganglia antibodies in PANDAS.</title><author><name sortKey="Singer, Harvey S" sort="Singer, Harvey S" uniqKey="Singer H" first="Harvey S" last="Singer">Harvey S. Singer</name><affiliation wicri:level="2"><nlm:affiliation>Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. hsinger@jhmi.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Loiselle, Christopher R" sort="Loiselle, Christopher R" uniqKey="Loiselle C" first="Christopher R" last="Loiselle">Christopher R. Loiselle</name></author><author><name sortKey="Lee, Olivia" sort="Lee, Olivia" uniqKey="Lee O" first="Olivia" last="Lee">Olivia Lee</name></author><author><name sortKey="Minzer, Karen" sort="Minzer, Karen" uniqKey="Minzer K" first="Karen" last="Minzer">Karen Minzer</name></author><author><name sortKey="Swedo, Susan" sort="Swedo, Susan" uniqKey="Swedo S" first="Susan" last="Swedo">Susan Swedo</name></author><author><name sortKey="Grus, Franz H" sort="Grus, Franz H" uniqKey="Grus F" first="Franz H" last="Grus">Franz H. Grus</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15077238</idno><idno type="pmid">15077238</idno><idno type="doi">10.1002/mds.20052</idno><idno type="wicri:Area/PubMed/Corpus">003491</idno><idno type="wicri:Area/PubMed/Curation">003491</idno><idno type="wicri:Area/PubMed/Checkpoint">003564</idno><idno type="wicri:Area/Ncbi/Merge">000D93</idno><idno type="wicri:Area/Ncbi/Curation">000D93</idno><idno type="wicri:Area/Ncbi/Checkpoint">000D93</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Anti-basal ganglia antibodies in PANDAS.</title><author><name sortKey="Singer, Harvey S" sort="Singer, Harvey S" uniqKey="Singer H" first="Harvey S" last="Singer">Harvey S. Singer</name><affiliation wicri:level="2"><nlm:affiliation>Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. hsinger@jhmi.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Loiselle, Christopher R" sort="Loiselle, Christopher R" uniqKey="Loiselle C" first="Christopher R" last="Loiselle">Christopher R. Loiselle</name></author><author><name sortKey="Lee, Olivia" sort="Lee, Olivia" uniqKey="Lee O" first="Olivia" last="Lee">Olivia Lee</name></author><author><name sortKey="Minzer, Karen" sort="Minzer, Karen" uniqKey="Minzer K" first="Karen" last="Minzer">Karen Minzer</name></author><author><name sortKey="Swedo, Susan" sort="Swedo, Susan" uniqKey="Swedo S" first="Susan" last="Swedo">Susan Swedo</name></author><author><name sortKey="Grus, Franz H" sort="Grus, Franz H" uniqKey="Grus F" first="Franz H" last="Grus">Franz H. Grus</name></author></analytic><series><title level="j">Movement disorders : official journal of the Movement Disorder Society</title><idno type="ISSN">0885-3185</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Antibodies, Anti-Idiotypic (immunology)</term><term>Autoimmune Diseases (immunology)</term><term>Autoimmune Diseases (microbiology)</term><term>Basal Ganglia (immunology)</term><term>Basal Ganglia (pathology)</term><term>Blotting, Western</term><term>Brain (immunology)</term><term>Brain (microbiology)</term><term>Child</term><term>Child, Preschool</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Globus Pallidus (immunology)</term><term>Globus Pallidus (pathology)</term><term>Humans</term><term>Male</term><term>Mental Disorders (microbiology)</term><term>Streptococcal Infections (complications)</term><term>Streptococcal Infections (immunology)</term><term>Tics (immunology)</term><term>Tics (microbiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Anti-Idiotypic</term></keywords><keywords scheme="MESH" qualifier="complications" xml:lang="en"><term>Streptococcal Infections</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Autoimmune Diseases</term><term>Basal Ganglia</term><term>Brain</term><term>Globus Pallidus</term><term>Streptococcal Infections</term><term>Tics</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Autoimmune Diseases</term><term>Brain</term><term>Mental Disorders</term><term>Tics</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Basal Ganglia</term><term>Globus Pallidus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Blotting, Western</term><term>Child</term><term>Child, Preschool</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Humans</term><term>Male</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An autoimmune-mediated mechanism involving molecular mimicry has been proposed for a variety of pediatric movement disorders that occur after a streptococcal infection. In this study, anti-basal ganglia antibodies (ABGA) were measured in 15 children with the diagnosis of pediatric autoimmune neuropsychiatric disorder associated with streptococcal infection (PANDAS) and compared with those in 15 controls. ELISA and Western immunoblotting (WB) methods were used to detect ABGA against supernatant (S1), pellet (P2), and synaptosomal preparations from adult postmortem caudate, putamen, and globus pallidus. ELISA optical density values did not differ between PANDAS patients and controls across all preparations. Immunoblotting identified multiple bands in all subjects with no differences in the number of bands or their total density. Discriminant analysis, used to assess mean binding patterns, showed that PANDAS patients differed from controls only for the caudate S1 fraction (Wilks' lambda = 0.0236, P < 0.0002), with PANDAS-primarily tic subjects providing the greatest discrimination. Among the epitopes contributing to differences between PANDAS and control in the caudate S1 fraction, mean binding to the epitope at 183 kDa was the most different between groups. In conclusion, ELISA measurements do not differentiate between PANDAS and controls, suggesting a lack of major antibody changes in this disorder. Further immunoblot analyses using a caudate supernatant fraction are required to completely exclude the possibility of minor antibody repertoire differences in PANDAS subjects, especially in those who primarily have tics.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><noCountry><name sortKey="Grus, Franz H" sort="Grus, Franz H" uniqKey="Grus F" first="Franz H" last="Grus">Franz H. Grus</name><name sortKey="Lee, Olivia" sort="Lee, Olivia" uniqKey="Lee O" first="Olivia" last="Lee">Olivia Lee</name><name sortKey="Loiselle, Christopher R" sort="Loiselle, Christopher R" uniqKey="Loiselle C" first="Christopher R" last="Loiselle">Christopher R. Loiselle</name><name sortKey="Minzer, Karen" sort="Minzer, Karen" uniqKey="Minzer K" first="Karen" last="Minzer">Karen Minzer</name><name sortKey="Swedo, Susan" sort="Swedo, Susan" uniqKey="Swedo S" first="Susan" last="Swedo">Susan Swedo</name></noCountry><country name="États-Unis"><region name="Maryland"><name sortKey="Singer, Harvey S" sort="Singer, Harvey S" uniqKey="Singer H" first="Harvey S" last="Singer">Harvey S. Singer</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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