Caspase‐3 activation in 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP)‐treated mice
Identifieur interne : 006D33 ( Main/Merge ); précédent : 006D32; suivant : 006D34Caspase‐3 activation in 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP)‐treated mice
Auteurs : Hélène Turmel [France] ; Andreas Hartmann [France] ; Karine Parain [France] ; Aicha Douhou [France] ; Anu Srinivasan [États-Unis] ; Yves Agid [France] ; Etienne C. Hirsch [France]Source :
- Movement Disorders [ 0885-3185 ] ; 2001-03.
English descriptors
- KwdEn :
Abstract
In 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP) models of Parkinson's disease (PD), dopaminergic (DA) neurons have been shown to die by apoptosis. Moreover, recent postmortem and in vitro results have indicated that apoptotic cell death induced by 1‐methyl‐4‐phenylpyridinium (MPP+) may be mediated by caspase‐3. To establish whether caspase‐3 activation may indeed play a role in an in vivo model of PD, we studied caspase‐3 activation in C57Bl/6 mice subchronically intoxicated with MPTP. We show that caspase‐3 activation peaks early, at days 1 and 2 after the end of MPTP intoxication. In contrast, pycnotic neurons persist until day 7 postintoxication, indicating that caspase‐3 activation is an early and transient phenomenon in apoptotic death of DA neurons. We further demonstrate that loss of tyrosine hydroxylase (TH) immunoreactivity in this model is indeed due to cell loss rather than to loss of TH protein expression. We conclude that mice subchronically intoxicated with MPTP represent a valid PD model to study and manipulate caspase activation in vivo. © 2001 Movement Disorder Society.
Url:
DOI: 10.1002/mds.1037
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 002D20
- to stream Istex, to step Curation: 002D20
- to stream Istex, to step Checkpoint: 003078
Links to Exploration step
ISTEX:919388BA1F3FE05BB4D9DE7516DA1DB6A839064BLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Caspase‐3 activation in 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP)‐treated mice</title>
<author><name sortKey="Turmel, Helene" sort="Turmel, Helene" uniqKey="Turmel H" first="Hélène" last="Turmel">Hélène Turmel</name>
</author>
<author><name sortKey="Hartmann, Andreas" sort="Hartmann, Andreas" uniqKey="Hartmann A" first="Andreas" last="Hartmann">Andreas Hartmann</name>
</author>
<author><name sortKey="Parain, Karine" sort="Parain, Karine" uniqKey="Parain K" first="Karine" last="Parain">Karine Parain</name>
</author>
<author><name sortKey="Douhou, Aicha" sort="Douhou, Aicha" uniqKey="Douhou A" first="Aicha" last="Douhou">Aicha Douhou</name>
</author>
<author><name sortKey="Srinivasan, Anu" sort="Srinivasan, Anu" uniqKey="Srinivasan A" first="Anu" last="Srinivasan">Anu Srinivasan</name>
</author>
<author><name sortKey="Agid, Yves" sort="Agid, Yves" uniqKey="Agid Y" first="Yves" last="Agid">Yves Agid</name>
<affiliation><country>France</country>
<placeName><settlement type="city">Paris</settlement>
<region type="region" nuts="2">Île-de-France</region>
</placeName>
<orgName type="hospital" n="4">Hôpital de la Salpêtrière</orgName>
</affiliation>
</author>
<author><name sortKey="Hirsch, Etienne C" sort="Hirsch, Etienne C" uniqKey="Hirsch E" first="Etienne C." last="Hirsch">Etienne C. Hirsch</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:919388BA1F3FE05BB4D9DE7516DA1DB6A839064B</idno>
<date when="2001" year="2001">2001</date>
<idno type="doi">10.1002/mds.1037</idno>
<idno type="url">https://api.istex.fr/document/919388BA1F3FE05BB4D9DE7516DA1DB6A839064B/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">002D20</idno>
<idno type="wicri:Area/Istex/Curation">002D20</idno>
<idno type="wicri:Area/Istex/Checkpoint">003078</idno>
<idno type="wicri:doubleKey">0885-3185:2001:Turmel H:caspase:activation:in</idno>
<idno type="wicri:Area/Main/Merge">006D33</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Caspase‐3 activation in 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP)‐treated mice</title>
<author><name sortKey="Turmel, Helene" sort="Turmel, Helene" uniqKey="Turmel H" first="Hélène" last="Turmel">Hélène Turmel</name>
<affiliation wicri:level="1"><country xml:lang="fr">France</country>
<wicri:regionArea>INSERM U 289, Hópital de la Salpétrière, Paris</wicri:regionArea>
<placeName><settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hartmann, Andreas" sort="Hartmann, Andreas" uniqKey="Hartmann A" first="Andreas" last="Hartmann">Andreas Hartmann</name>
<affiliation wicri:level="1"><country xml:lang="fr">France</country>
<wicri:regionArea>INSERM U 289, Hópital de la Salpétrière, Paris</wicri:regionArea>
<placeName><settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Parain, Karine" sort="Parain, Karine" uniqKey="Parain K" first="Karine" last="Parain">Karine Parain</name>
<affiliation wicri:level="1"><country xml:lang="fr">France</country>
<wicri:regionArea>INSERM U 289, Hópital de la Salpétrière, Paris</wicri:regionArea>
<placeName><settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Douhou, Aicha" sort="Douhou, Aicha" uniqKey="Douhou A" first="Aicha" last="Douhou">Aicha Douhou</name>
<affiliation wicri:level="1"><country xml:lang="fr">France</country>
<wicri:regionArea>INSERM U 289, Hópital de la Salpétrière, Paris</wicri:regionArea>
<placeName><settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Srinivasan, Anu" sort="Srinivasan, Anu" uniqKey="Srinivasan A" first="Anu" last="Srinivasan">Anu Srinivasan</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>IDUN Pharmaceuticals Inc., La Jolla, California</wicri:regionArea>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Agid, Yves" sort="Agid, Yves" uniqKey="Agid Y" first="Yves" last="Agid">Yves Agid</name>
<affiliation wicri:level="1"><country xml:lang="fr">France</country>
<wicri:regionArea>INSERM U 289, Hópital de la Salpétrière, Paris</wicri:regionArea>
<placeName><settlement type="city">Paris</settlement>
</placeName>
<placeName><settlement type="city">Paris</settlement>
<region type="region" nuts="2">Île-de-France</region>
</placeName>
<orgName type="hospital" n="4">Hôpital de la Salpêtrière</orgName>
</affiliation>
</author>
<author><name sortKey="Hirsch, Etienne C" sort="Hirsch, Etienne C" uniqKey="Hirsch E" first="Etienne C." last="Hirsch">Etienne C. Hirsch</name>
<affiliation wicri:level="1"><country xml:lang="fr">France</country>
<wicri:regionArea>INSERM U 289, Hópital de la Salpétrière, Paris</wicri:regionArea>
<placeName><settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Movement Disorders</title>
<title level="j" type="sub">Official Journal of the Movement Disorder Society</title>
<title level="j" type="abbrev">Mov. Disord.</title>
<idno type="ISSN">0885-3185</idno>
<idno type="eISSN">1531-8257</idno>
<imprint><publisher>John Wiley & Sons, Inc.</publisher>
<pubPlace>New York</pubPlace>
<date type="published" when="2001-03">2001-03</date>
<biblScope unit="vol">16</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="185">185</biblScope>
<biblScope unit="page" to="189">189</biblScope>
</imprint>
<idno type="ISSN">0885-3185</idno>
</series>
<idno type="istex">919388BA1F3FE05BB4D9DE7516DA1DB6A839064B</idno>
<idno type="DOI">10.1002/mds.1037</idno>
<idno type="ArticleID">MDS1037</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0885-3185</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>MPP+</term>
<term>MPTP</term>
<term>Parkinson's disease</term>
<term>apoptosis</term>
<term>caspase‐3</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">In 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP) models of Parkinson's disease (PD), dopaminergic (DA) neurons have been shown to die by apoptosis. Moreover, recent postmortem and in vitro results have indicated that apoptotic cell death induced by 1‐methyl‐4‐phenylpyridinium (MPP+) may be mediated by caspase‐3. To establish whether caspase‐3 activation may indeed play a role in an in vivo model of PD, we studied caspase‐3 activation in C57Bl/6 mice subchronically intoxicated with MPTP. We show that caspase‐3 activation peaks early, at days 1 and 2 after the end of MPTP intoxication. In contrast, pycnotic neurons persist until day 7 postintoxication, indicating that caspase‐3 activation is an early and transient phenomenon in apoptotic death of DA neurons. We further demonstrate that loss of tyrosine hydroxylase (TH) immunoreactivity in this model is indeed due to cell loss rather than to loss of TH protein expression. We conclude that mice subchronically intoxicated with MPTP represent a valid PD model to study and manipulate caspase activation in vivo. © 2001 Movement Disorder Society.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Santé/explor/MovDisordV3/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 006D33 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 006D33 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Santé |area= MovDisordV3 |flux= Main |étape= Merge |type= RBID |clé= ISTEX:919388BA1F3FE05BB4D9DE7516DA1DB6A839064B |texte= Caspase‐3 activation in 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP)‐treated mice }}
This area was generated with Dilib version V0.6.23. |