Synthesis of DNA fragments containing 5,6-dihydrothymine, a major product of thymine gamma radiolysis.
Identifieur interne : 002A68 ( PubMed/Curation ); précédent : 002A67; suivant : 002A69Synthesis of DNA fragments containing 5,6-dihydrothymine, a major product of thymine gamma radiolysis.
Auteurs : J C Schulhof [France] ; D. Molko ; R. TeouleSource :
- Nucleic acids research [ 0305-1048 ] ; 1988.
Descripteurs français
- KwdFr :
- MESH :
- analogues et dérivés : Thymine.
- synthèse chimique : ADN, Oligodésoxyribonucléotides.
- Indicateurs et réactifs, Spectrométrie de masse.
English descriptors
- KwdEn :
- MESH :
- chemical , analogs & derivatives : Thymine.
- chemical , chemical synthesis : DNA, Oligodeoxyribonucleotides.
- chemical : Indicators and Reagents.
- Mass Spectrometry.
Abstract
5,6-Dihydrothymine is one of the most important products of base damage by gamma irradiation of DNA in anoxic conditions. This modified base is unstable in the deprotection conditions used for classical synthesis of oligonucleotides. For its incorporation in synthetic DNA fragments, a new set of amino protecting groups has been developed. The 5,6-dihydrothymidine phosphoramidite was successfully employed for the synthesis of two 14-mers and one 17-mer bearing this defect at positions corresponding to restriction enzymes sites. The presence of the modified base still intact in the oligonucleotides was evidenced by mass spectrometry in pyrolytic conditions.
DOI: 10.1093/nar/16.1.319
PubMed: 3340528
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002A68
Links to Exploration step
pubmed:3340528Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Synthesis of DNA fragments containing 5,6-dihydrothymine, a major product of thymine gamma radiolysis.</title>
<author><name sortKey="Schulhof, J C" sort="Schulhof, J C" uniqKey="Schulhof J" first="J C" last="Schulhof">J C Schulhof</name>
<affiliation wicri:level="1"><nlm:affiliation>Département de Recherche Fondamentale de Grenoble, Centre d'Etudes, Nucléaires de Grenoble, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Département de Recherche Fondamentale de Grenoble, Centre d'Etudes, Nucléaires de Grenoble</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Molko, D" sort="Molko, D" uniqKey="Molko D" first="D" last="Molko">D. Molko</name>
</author>
<author><name sortKey="Teoule, R" sort="Teoule, R" uniqKey="Teoule R" first="R" last="Teoule">R. Teoule</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="1988">1988</date>
<idno type="RBID">pubmed:3340528</idno>
<idno type="pmid">3340528</idno>
<idno type="doi">10.1093/nar/16.1.319</idno>
<idno type="wicri:Area/PubMed/Corpus">002A68</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002A68</idno>
<idno type="wicri:Area/PubMed/Curation">002A68</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002A68</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Synthesis of DNA fragments containing 5,6-dihydrothymine, a major product of thymine gamma radiolysis.</title>
<author><name sortKey="Schulhof, J C" sort="Schulhof, J C" uniqKey="Schulhof J" first="J C" last="Schulhof">J C Schulhof</name>
<affiliation wicri:level="1"><nlm:affiliation>Département de Recherche Fondamentale de Grenoble, Centre d'Etudes, Nucléaires de Grenoble, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Département de Recherche Fondamentale de Grenoble, Centre d'Etudes, Nucléaires de Grenoble</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Molko, D" sort="Molko, D" uniqKey="Molko D" first="D" last="Molko">D. Molko</name>
</author>
<author><name sortKey="Teoule, R" sort="Teoule, R" uniqKey="Teoule R" first="R" last="Teoule">R. Teoule</name>
</author>
</analytic>
<series><title level="j">Nucleic acids research</title>
<idno type="ISSN">0305-1048</idno>
<imprint><date when="1988" type="published">1988</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA (chemical synthesis)</term>
<term>Indicators and Reagents</term>
<term>Mass Spectrometry</term>
<term>Oligodeoxyribonucleotides (chemical synthesis)</term>
<term>Thymine (analogs & derivatives)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN (synthèse chimique)</term>
<term>Indicateurs et réactifs</term>
<term>Oligodésoxyribonucléotides (synthèse chimique)</term>
<term>Spectrométrie de masse</term>
<term>Thymine (analogues et dérivés)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Thymine</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>DNA</term>
<term>Oligodeoxyribonucleotides</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Indicators and Reagents</term>
</keywords>
<keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Thymine</term>
</keywords>
<keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>ADN</term>
<term>Oligodésoxyribonucléotides</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Mass Spectrometry</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Indicateurs et réactifs</term>
<term>Spectrométrie de masse</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">5,6-Dihydrothymine is one of the most important products of base damage by gamma irradiation of DNA in anoxic conditions. This modified base is unstable in the deprotection conditions used for classical synthesis of oligonucleotides. For its incorporation in synthetic DNA fragments, a new set of amino protecting groups has been developed. The 5,6-dihydrothymidine phosphoramidite was successfully employed for the synthesis of two 14-mers and one 17-mer bearing this defect at positions corresponding to restriction enzymes sites. The presence of the modified base still intact in the oligonucleotides was evidenced by mass spectrometry in pyrolytic conditions.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">3340528</PMID>
<DateCompleted><Year>1988</Year>
<Month>03</Month>
<Day>07</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>05</Month>
<Day>01</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0305-1048</ISSN>
<JournalIssue CitedMedium="Print"><Volume>16</Volume>
<Issue>1</Issue>
<PubDate><Year>1988</Year>
<Month>Jan</Month>
<Day>11</Day>
</PubDate>
</JournalIssue>
<Title>Nucleic acids research</Title>
<ISOAbbreviation>Nucleic Acids Res.</ISOAbbreviation>
</Journal>
<ArticleTitle>Synthesis of DNA fragments containing 5,6-dihydrothymine, a major product of thymine gamma radiolysis.</ArticleTitle>
<Pagination><MedlinePgn>319-26</MedlinePgn>
</Pagination>
<Abstract><AbstractText>5,6-Dihydrothymine is one of the most important products of base damage by gamma irradiation of DNA in anoxic conditions. This modified base is unstable in the deprotection conditions used for classical synthesis of oligonucleotides. For its incorporation in synthetic DNA fragments, a new set of amino protecting groups has been developed. The 5,6-dihydrothymidine phosphoramidite was successfully employed for the synthesis of two 14-mers and one 17-mer bearing this defect at positions corresponding to restriction enzymes sites. The presence of the modified base still intact in the oligonucleotides was evidenced by mass spectrometry in pyrolytic conditions.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Schulhof</LastName>
<ForeName>J C</ForeName>
<Initials>JC</Initials>
<AffiliationInfo><Affiliation>Département de Recherche Fondamentale de Grenoble, Centre d'Etudes, Nucléaires de Grenoble, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Molko</LastName>
<ForeName>D</ForeName>
<Initials>D</Initials>
</Author>
<Author ValidYN="Y"><LastName>Teoule</LastName>
<ForeName>R</ForeName>
<Initials>R</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Nucleic Acids Res</MedlineTA>
<NlmUniqueID>0411011</NlmUniqueID>
<ISSNLinking>0305-1048</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D007202">Indicators and Reagents</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D009838">Oligodeoxyribonucleotides</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>696-04-8</RegistryNumber>
<NameOfSubstance UI="C042836">5,6-dihydrothymine</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9007-49-2</RegistryNumber>
<NameOfSubstance UI="D004247">DNA</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>QR26YLT7LT</RegistryNumber>
<NameOfSubstance UI="D013941">Thymine</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D004247" MajorTopicYN="N">DNA</DescriptorName>
<QualifierName UI="Q000138" MajorTopicYN="Y">chemical synthesis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007202" MajorTopicYN="N">Indicators and Reagents</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013058" MajorTopicYN="N">Mass Spectrometry</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009838" MajorTopicYN="N">Oligodeoxyribonucleotides</DescriptorName>
<QualifierName UI="Q000138" MajorTopicYN="Y">chemical synthesis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013941" MajorTopicYN="N">Thymine</DescriptorName>
<QualifierName UI="Q000031" MajorTopicYN="Y">analogs & derivatives</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1988</Year>
<Month>1</Month>
<Day>11</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>1988</Year>
<Month>1</Month>
<Day>11</Day>
<Hour>0</Hour>
<Minute>1</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>1988</Year>
<Month>1</Month>
<Day>11</Day>
<Hour>0</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">3340528</ArticleId>
<ArticleId IdType="pmc">PMC334629</ArticleId>
<ArticleId IdType="doi">10.1093/nar/16.1.319</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>C R Acad Sci Hebd Seances Acad Sci D. 1974 Dec 2;279(23):1797-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">4218540</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1976 Nov;3(11):2959-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1005110</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Enzymol. 1980;65(1):499-560</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6246368</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1982 May 25;10(10):3249-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7099961</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1982 Nov 11;10(21):6675-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7177853</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1984 Jun 11;12(11):4539-57</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6547529</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 1987 Apr 21;26(8):2232-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3040084</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1986 Oct 10;14(19):7661-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3774542</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1987 Jan 12;15(1):219-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3029671</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1987 Jan 26;15(2):397-416</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3822812</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1987 Mar 11;15(5):2191-201</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3562225</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 1987 Feb 10;26(3):964-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3552040</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 1987 Feb 24;26(4):1086-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3567156</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochimie. 1985 Jul-Aug;67(7-8):801-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">4084602</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002A68 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 002A68 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= PubMed |étape= Curation |type= RBID |clé= pubmed:3340528 |texte= Synthesis of DNA fragments containing 5,6-dihydrothymine, a major product of thymine gamma radiolysis. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i -Sk "pubmed:3340528" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
This area was generated with Dilib version V0.6.33. |