Co-operative autoregulation of a replication protein gene.
Identifieur interne : 002972 ( PubMed/Curation ); précédent : 002971; suivant : 002973Co-operative autoregulation of a replication protein gene.
Auteurs : A E Gammie ; J H CrosaSource :
- Molecular microbiology [ 0950-382X ] ; 1991.
Descripteurs français
- KwdFr :
- Activation de la transcription, Analyse de mutations d'ADN, Données de séquences moléculaires, Plasmides (génétique), Protéines bactériennes (génétique), Protéines de liaison à l'ADN (génétique), Protéines de répression (génétique), Régions promotrices (génétique) (génétique), Régulation de l'expression des gènes bactériens, Réplication de l'ADN (génétique), Réplicon (génétique), Similitude de séquences d'acides nucléiques, Séquence nucléotidique, Transactivateurs.
- MESH :
- génétique : Plasmides, Protéines bactériennes, Protéines de liaison à l'ADN, Protéines de répression, Régions promotrices (génétique), Réplication de l'ADN, Réplicon.
- Activation de la transcription, Analyse de mutations d'ADN, Données de séquences moléculaires, Régulation de l'expression des gènes bactériens, Similitude de séquences d'acides nucléiques, Séquence nucléotidique, Transactivateurs.
English descriptors
- KwdEn :
- Bacterial Proteins (genetics), Base Sequence, DNA Mutational Analysis, DNA Replication (genetics), DNA-Binding Proteins (genetics), Gene Expression Regulation, Bacterial, Molecular Sequence Data, Plasmids (genetics), Promoter Regions, Genetic (genetics), Replicon (genetics), Repressor Proteins (genetics), Sequence Homology, Nucleic Acid, Trans-Activators, Transcriptional Activation.
- MESH :
- chemical , genetics : Bacterial Proteins, DNA-Binding Proteins, Repressor Proteins.
- genetics : DNA Replication, Plasmids, Promoter Regions, Genetic, Replicon.
- Base Sequence, DNA Mutational Analysis, Gene Expression Regulation, Bacterial, Molecular Sequence Data, Sequence Homology, Nucleic Acid, Trans-Activators, Transcriptional Activation.
Abstract
In this work we present the localization and characterization of the repl promoter (Prepl) and show aspects of the regulation. Comparison of Prepl with other autoregulated replication protein gene promoters revealed similarities, but Prepl differs from some of these characterized promoters in not being regulated by the heat-shock RNA polymerase. Primer extension analysis showed that Prepl is contained within five helically aligned 18 base pair repeats, or 18-mers of the previously defined minimal origin. In addition, we find that Prepl is autoregulated by a trans-acting product encoded in the REPI region. Purified Repl protein binds to the 18-mer region of the origin, suggesting that the repl gene is autoregulated by the protein product. The autoregulation appears to be co-operative since decreasing the 18-mer binding site region results in a concomitant non-linear loss of autorepression. The deletion derivatives show a decreased ability to bind the Repl protein when compared with origin DNA containing all of the binding region. The diminished capacity of the various deletion derivatives to bind Repl in vitro correlates with the loss of autorepression seen in vivo.
DOI: 10.1111/j.1365-2958.1991.tb01861.x
PubMed: 1809840
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A E Gammie<affiliation><nlm:affiliation>Department of Microbiology and Immunology, Oregon Health Sciences University, Portland 97201.</nlm:affiliation><wicri:noCountry code="subField">Portland 97201</wicri:noCountry></affiliation>Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Co-operative autoregulation of a replication protein gene.</title><author><name sortKey="Gammie, A E" sort="Gammie, A E" uniqKey="Gammie A" first="A E" last="Gammie">A E Gammie</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, Oregon Health Sciences University, Portland 97201.</nlm:affiliation><wicri:noCountry code="subField">Portland 97201</wicri:noCountry></affiliation></author><author><name sortKey="Crosa, J H" sort="Crosa, J H" uniqKey="Crosa J" first="J H" last="Crosa">J H Crosa</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1991">1991</date><idno type="RBID">pubmed:1809840</idno><idno type="pmid">1809840</idno><idno type="doi">10.1111/j.1365-2958.1991.tb01861.x</idno><idno type="wicri:Area/PubMed/Corpus">002972</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002972</idno><idno type="wicri:Area/PubMed/Curation">002972</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002972</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Co-operative autoregulation of a replication protein gene.</title><author><name sortKey="Gammie, A E" sort="Gammie, A E" uniqKey="Gammie A" first="A E" last="Gammie">A E Gammie</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, Oregon Health Sciences University, Portland 97201.</nlm:affiliation><wicri:noCountry code="subField">Portland 97201</wicri:noCountry></affiliation></author><author><name sortKey="Crosa, J H" sort="Crosa, J H" uniqKey="Crosa J" first="J H" last="Crosa">J H Crosa</name></author></analytic><series><title level="j">Molecular microbiology</title><idno type="ISSN">0950-382X</idno><imprint><date when="1991" type="published">1991</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Proteins (genetics)</term><term>Base Sequence</term><term>DNA Mutational Analysis</term><term>DNA Replication (genetics)</term><term>DNA-Binding Proteins (genetics)</term><term>Gene Expression Regulation, Bacterial</term><term>Molecular Sequence Data</term><term>Plasmids (genetics)</term><term>Promoter Regions, Genetic (genetics)</term><term>Replicon (genetics)</term><term>Repressor Proteins (genetics)</term><term>Sequence Homology, Nucleic Acid</term><term>Trans-Activators</term><term>Transcriptional Activation</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation de la transcription</term><term>Analyse de mutations d'ADN</term><term>Données de séquences moléculaires</term><term>Plasmides (génétique)</term><term>Protéines bactériennes (génétique)</term><term>Protéines de liaison à l'ADN (génétique)</term><term>Protéines de répression (génétique)</term><term>Régions promotrices (génétique) (génétique)</term><term>Régulation de l'expression des gènes bactériens</term><term>Réplication de l'ADN (génétique)</term><term>Réplicon (génétique)</term><term>Similitude de séquences d'acides nucléiques</term><term>Séquence nucléotidique</term><term>Transactivateurs</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>DNA-Binding Proteins</term><term>Repressor Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>DNA Replication</term><term>Plasmids</term><term>Promoter Regions, Genetic</term><term>Replicon</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Plasmides</term><term>Protéines bactériennes</term><term>Protéines de liaison à l'ADN</term><term>Protéines de répression</term><term>Régions promotrices (génétique)</term><term>Réplication de l'ADN</term><term>Réplicon</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>DNA Mutational Analysis</term><term>Gene Expression Regulation, Bacterial</term><term>Molecular Sequence Data</term><term>Sequence Homology, Nucleic Acid</term><term>Trans-Activators</term><term>Transcriptional Activation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Activation de la transcription</term><term>Analyse de mutations d'ADN</term><term>Données de séquences moléculaires</term><term>Régulation de l'expression des gènes bactériens</term><term>Similitude de séquences d'acides nucléiques</term><term>Séquence nucléotidique</term><term>Transactivateurs</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this work we present the localization and characterization of the repl promoter (Prepl) and show aspects of the regulation. Comparison of Prepl with other autoregulated replication protein gene promoters revealed similarities, but Prepl differs from some of these characterized promoters in not being regulated by the heat-shock RNA polymerase. Primer extension analysis showed that Prepl is contained within five helically aligned 18 base pair repeats, or 18-mers of the previously defined minimal origin. In addition, we find that Prepl is autoregulated by a trans-acting product encoded in the REPI region. Purified Repl protein binds to the 18-mer region of the origin, suggesting that the repl gene is autoregulated by the protein product. The autoregulation appears to be co-operative since decreasing the 18-mer binding site region results in a concomitant non-linear loss of autorepression. The deletion derivatives show a decreased ability to bind the Repl protein when compared with origin DNA containing all of the binding region. The diminished capacity of the various deletion derivatives to bind Repl in vitro correlates with the loss of autorepression seen in vivo.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1809840</PMID><DateCompleted><Year>1992</Year><Month>05</Month><Day>27</Day></DateCompleted><DateRevised><Year>2019</Year><Month>07</Month><Day>25</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0950-382X</ISSN><JournalIssue CitedMedium="Print"><Volume>5</Volume><Issue>12</Issue><PubDate><Year>1991</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Molecular microbiology</Title><ISOAbbreviation>Mol. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Co-operative autoregulation of a replication protein gene.</ArticleTitle><Pagination><MedlinePgn>3015-23</MedlinePgn></Pagination><Abstract><AbstractText>In this work we present the localization and characterization of the repl promoter (Prepl) and show aspects of the regulation. Comparison of Prepl with other autoregulated replication protein gene promoters revealed similarities, but Prepl differs from some of these characterized promoters in not being regulated by the heat-shock RNA polymerase. Primer extension analysis showed that Prepl is contained within five helically aligned 18 base pair repeats, or 18-mers of the previously defined minimal origin. In addition, we find that Prepl is autoregulated by a trans-acting product encoded in the REPI region. Purified Repl protein binds to the 18-mer region of the origin, suggesting that the repl gene is autoregulated by the protein product. The autoregulation appears to be co-operative since decreasing the 18-mer binding site region results in a concomitant non-linear loss of autorepression. The deletion derivatives show a decreased ability to bind the Repl protein when compared with origin DNA containing all of the binding region. The diminished capacity of the various deletion derivatives to bind Repl in vitro correlates with the loss of autorepression seen in vivo.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gammie</LastName><ForeName>A E</ForeName><Initials>AE</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, Oregon Health Sciences University, Portland 97201.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Crosa</LastName><ForeName>J H</ForeName><Initials>JH</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>M80525</AccessionNumber><AccessionNumber>S77307</AccessionNumber><AccessionNumber>X52368</AccessionNumber><AccessionNumber>X52369</AccessionNumber><AccessionNumber>X52370</AccessionNumber><AccessionNumber>X52371</AccessionNumber><AccessionNumber>X52372</AccessionNumber><AccessionNumber>X58355</AccessionNumber><AccessionNumber>X59272</AccessionNumber><AccessionNumber>X59968</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mol Microbiol</MedlineTA><NlmUniqueID>8712028</NlmUniqueID><ISSNLinking>0950-382X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004268">DNA-Binding Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C069089">RepI protein, Plasmid ColV-K30</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012097">Repressor Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015534">Trans-Activators</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004252" MajorTopicYN="N">DNA Mutational Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004261" MajorTopicYN="N">DNA Replication</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004268" MajorTopicYN="N">DNA-Binding Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015964" MajorTopicYN="Y">Gene Expression Regulation, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012093" MajorTopicYN="N">Replicon</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012097" MajorTopicYN="N">Repressor Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012689" MajorTopicYN="N">Sequence Homology, Nucleic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015534" MajorTopicYN="Y">Trans-Activators</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015533" MajorTopicYN="N">Transcriptional Activation</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1991</Year><Month>12</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1991</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1991</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">1809840</ArticleId><ArticleId IdType="doi">10.1111/j.1365-2958.1991.tb01861.x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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