Photomodification of RNA and DNA fragments by oligonucleotide reagents bearing arylazide groups.
Identifieur interne : 002935 ( PubMed/Curation ); précédent : 002934; suivant : 002936Photomodification of RNA and DNA fragments by oligonucleotide reagents bearing arylazide groups.
Auteurs : A S Levina ; M V Berezovskii ; A G Venjaminova ; M I Dobrikov ; M N Repkova ; V F ZarytovaSource :
- Biochimie [ 0300-9084 ] ; 1993.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Azides, DNA, Oligonucleotides, RNA.
- chemical : Indicators and Reagents.
- Photochemistry.
Abstract
Photomodification of ribo- and deoxyribo-octanucleotides by oligonucleotide reagents (6- and 7-mers) bearing p-azidotetrafluorobenzamido and 2-nitro-5-azidobenzamido groups has been investigated. It is shown that the oligonucleotides with a perfluoroarylazide group were effective modifiers both of deoxyribo- and ribo-targets. Maximum extent of cross-linked product formation (70%) was obtained when the deoxyribo-octanucleotide was modified by a heptanucleotide reagent with a perfluoroarylazide group. Selectivity of the photomodification was also high (50% on the G-residue at a certain position).
DOI: 10.1016/0300-9084(93)90020-s
PubMed: 7684935
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A S Levina<affiliation><nlm:affiliation>Institute of Bioorganic Chemistry, Siberian Division of the Russian Academy of Sciences, Novosibirsk.</nlm:affiliation>
<wicri:noCountry code="subField">Novosibirsk</wicri:noCountry>
</affiliation>
Le document en format XML
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<front><div type="abstract" xml:lang="en">Photomodification of ribo- and deoxyribo-octanucleotides by oligonucleotide reagents (6- and 7-mers) bearing p-azidotetrafluorobenzamido and 2-nitro-5-azidobenzamido groups has been investigated. It is shown that the oligonucleotides with a perfluoroarylazide group were effective modifiers both of deoxyribo- and ribo-targets. Maximum extent of cross-linked product formation (70%) was obtained when the deoxyribo-octanucleotide was modified by a heptanucleotide reagent with a perfluoroarylazide group. Selectivity of the photomodification was also high (50% on the G-residue at a certain position).</div>
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<Abstract><AbstractText>Photomodification of ribo- and deoxyribo-octanucleotides by oligonucleotide reagents (6- and 7-mers) bearing p-azidotetrafluorobenzamido and 2-nitro-5-azidobenzamido groups has been investigated. It is shown that the oligonucleotides with a perfluoroarylazide group were effective modifiers both of deoxyribo- and ribo-targets. Maximum extent of cross-linked product formation (70%) was obtained when the deoxyribo-octanucleotide was modified by a heptanucleotide reagent with a perfluoroarylazide group. Selectivity of the photomodification was also high (50% on the G-residue at a certain position).</AbstractText>
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