[Studies on mimotopes of hepatitis C virus E1 protein].
Identifieur interne : 002540 ( PubMed/Curation ); précédent : 002539; suivant : 002541[Studies on mimotopes of hepatitis C virus E1 protein].
Auteurs : F. Cao [République populaire de Chine] ; X. Shi ; Y. Du ; L. Hou ; Y. Ji ; H. WangSource :
- Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology [ 1007-3418 ] ; 2001.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ADN viral.
- génétique : Protéines de l'enveloppe virale.
- immunologie : Motifs d'acides aminés, Protéines de l'enveloppe virale, Épitopes.
- Banque de peptides, Données de séquences moléculaires, Séquence d'acides aminés, Séquence nucléotidique, Test ELISA.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : DNA, Viral.
- chemical , genetics : Viral Envelope Proteins.
- immunology : Amino Acid Motifs, Epitopes, Viral Envelope Proteins.
- Amino Acid Sequence, Base Sequence, Enzyme-Linked Immunosorbent Assay, Molecular Sequence Data, Peptide Library.
Abstract
To study the B-cell epitope of E1 protein of hepatitis C virus.
PubMed: 11602054
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pubmed:11602054Le document en format XML
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<author><name sortKey="Cao, F" sort="Cao, F" uniqKey="Cao F" first="F" last="Cao">F. Cao</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union College. Chengdu 610081, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union College. Chengdu 610081</wicri:regionArea>
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</author>
<author><name sortKey="Shi, X" sort="Shi, X" uniqKey="Shi X" first="X" last="Shi">X. Shi</name>
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<author><name sortKey="Du, Y" sort="Du, Y" uniqKey="Du Y" first="Y" last="Du">Y. Du</name>
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<author><name sortKey="Hou, L" sort="Hou, L" uniqKey="Hou L" first="L" last="Hou">L. Hou</name>
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<author><name sortKey="Ji, Y" sort="Ji, Y" uniqKey="Ji Y" first="Y" last="Ji">Y. Ji</name>
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<author><name sortKey="Wang, H" sort="Wang, H" uniqKey="Wang H" first="H" last="Wang">H. Wang</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[Studies on mimotopes of hepatitis C virus E1 protein].</title>
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<affiliation wicri:level="1"><nlm:affiliation>Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union College. Chengdu 610081, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
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<author><name sortKey="Du, Y" sort="Du, Y" uniqKey="Du Y" first="Y" last="Du">Y. Du</name>
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<author><name sortKey="Hou, L" sort="Hou, L" uniqKey="Hou L" first="L" last="Hou">L. Hou</name>
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<author><name sortKey="Ji, Y" sort="Ji, Y" uniqKey="Ji Y" first="Y" last="Ji">Y. Ji</name>
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<author><name sortKey="Wang, H" sort="Wang, H" uniqKey="Wang H" first="H" last="Wang">H. Wang</name>
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<series><title level="j">Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology</title>
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<imprint><date when="2001" type="published">2001</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Motifs (immunology)</term>
<term>Amino Acid Sequence</term>
<term>Base Sequence</term>
<term>DNA, Viral (analysis)</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Epitopes (immunology)</term>
<term>Molecular Sequence Data</term>
<term>Peptide Library</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (analyse)</term>
<term>Banque de peptides</term>
<term>Données de séquences moléculaires</term>
<term>Motifs d'acides aminés (immunologie)</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Test ELISA</term>
<term>Épitopes (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN viral</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de l'enveloppe virale</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Motifs d'acides aminés</term>
<term>Protéines de l'enveloppe virale</term>
<term>Épitopes</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Amino Acid Motifs</term>
<term>Epitopes</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Base Sequence</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Molecular Sequence Data</term>
<term>Peptide Library</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Banque de peptides</term>
<term>Données de séquences moléculaires</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Test ELISA</term>
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<front><div type="abstract" xml:lang="en">To study the B-cell epitope of E1 protein of hepatitis C virus.</div>
</front>
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<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11602054</PMID>
<DateCompleted><Year>2001</Year>
<Month>12</Month>
<Day>07</Day>
</DateCompleted>
<DateRevised><Year>2006</Year>
<Month>11</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1007-3418</ISSN>
<JournalIssue CitedMedium="Print"><Volume>9</Volume>
<Issue>4</Issue>
<PubDate><Year>2001</Year>
<Month>Aug</Month>
</PubDate>
</JournalIssue>
<Title>Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology</Title>
<ISOAbbreviation>Zhonghua Gan Zang Bing Za Zhi</ISOAbbreviation>
</Journal>
<ArticleTitle>[Studies on mimotopes of hepatitis C virus E1 protein].</ArticleTitle>
<Pagination><MedlinePgn>223-5</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To study the B-cell epitope of E1 protein of hepatitis C virus.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">By induction of IPTG, the E.coli M15 strains harboring the pQE30-HCVe118 expressed truncated C-terminal HCV E1 protein (Pte1). The proteins were purified with preparative electrophoreses system, which captured anti-E1 IgG in HCV (+) sera. By applying the antibodies as selective molecular 12 mers random peptide libraries were panned, and positive clones were obtained by ELISA. Amino acid sequences of display peptide were compared with that of HCV E1 protein.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">The purified HCV E1 proteins could react specifically with partly anti-HCV sera by ELISA. Among 10 phage display peptides, 6, 6, 2 were the most homologous to HCV E1 protein at position 320-336aa, 251-263aa, 225-248, respectively.</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">There exist multiple B-cell epitopes in HCV E1 protein. At least one preponderant epitope is mapped at residues 320-336 of HCV E1 protein.</AbstractText>
</Abstract>
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<AffiliationInfo><Affiliation>Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union College. Chengdu 610081, China.</Affiliation>
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<Author ValidYN="Y"><LastName>Du</LastName>
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<MedlineJournalInfo><Country>China</Country>
<MedlineTA>Zhonghua Gan Zang Bing Za Zhi</MedlineTA>
<NlmUniqueID>9710009</NlmUniqueID>
<ISSNLinking>1007-3418</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D004279">DNA, Viral</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C087035">E1 protein, Hepatitis C virus</NameOfSubstance>
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<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000939">Epitopes</NameOfSubstance>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D020816" MajorTopicYN="N">Amino Acid Motifs</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName>
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<MeshHeading><DescriptorName UI="D004279" MajorTopicYN="N">DNA, Viral</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000939" MajorTopicYN="N">Epitopes</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
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<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019151" MajorTopicYN="N">Peptide Library</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
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