Expression of c-myc is not critical for cell proliferation in established human leukemia lines.
Identifieur interne : 002528 ( PubMed/Curation ); précédent : 002527; suivant : 002529Expression of c-myc is not critical for cell proliferation in established human leukemia lines.
Auteurs : D M Tidd [Royaume-Uni] ; R V Giles ; C M Broughton ; R E ClarkSource :
- BMC molecular biology [ 1471-2199 ] ; 2001.
Abstract
A study was undertaken to resolve preliminary conflicting results on the proliferation of leukemia cells observed with different c-myc antisense oligonucleotides.
DOI: 10.1186/1471-2199-2-13
PubMed: 11734062
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002528
Links to Exploration step
pubmed:11734062Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Expression of c-myc is not critical for cell proliferation in established human leukemia lines.</title>
<author><name sortKey="Tidd, D M" sort="Tidd, D M" uniqKey="Tidd D" first="D M" last="Tidd">D M Tidd</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, The University of Liverpool, Liverpool, UK. dmtidd@liv.ac.uk</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>School of Biological Sciences, The University of Liverpool, Liverpool</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Giles, R V" sort="Giles, R V" uniqKey="Giles R" first="R V" last="Giles">R V Giles</name>
</author>
<author><name sortKey="Broughton, C M" sort="Broughton, C M" uniqKey="Broughton C" first="C M" last="Broughton">C M Broughton</name>
</author>
<author><name sortKey="Clark, R E" sort="Clark, R E" uniqKey="Clark R" first="R E" last="Clark">R E Clark</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2001">2001</date>
<idno type="RBID">pubmed:11734062</idno>
<idno type="pmid">11734062</idno>
<idno type="doi">10.1186/1471-2199-2-13</idno>
<idno type="wicri:Area/PubMed/Corpus">002528</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002528</idno>
<idno type="wicri:Area/PubMed/Curation">002528</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002528</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Expression of c-myc is not critical for cell proliferation in established human leukemia lines.</title>
<author><name sortKey="Tidd, D M" sort="Tidd, D M" uniqKey="Tidd D" first="D M" last="Tidd">D M Tidd</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, The University of Liverpool, Liverpool, UK. dmtidd@liv.ac.uk</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>School of Biological Sciences, The University of Liverpool, Liverpool</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Giles, R V" sort="Giles, R V" uniqKey="Giles R" first="R V" last="Giles">R V Giles</name>
</author>
<author><name sortKey="Broughton, C M" sort="Broughton, C M" uniqKey="Broughton C" first="C M" last="Broughton">C M Broughton</name>
</author>
<author><name sortKey="Clark, R E" sort="Clark, R E" uniqKey="Clark R" first="R E" last="Clark">R E Clark</name>
</author>
</analytic>
<series><title level="j">BMC molecular biology</title>
<idno type="ISSN">1471-2199</idno>
<imprint><date when="2001" type="published">2001</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A study was undertaken to resolve preliminary conflicting results on the proliferation of leukemia cells observed with different c-myc antisense oligonucleotides.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">11734062</PMID>
<DateCompleted><Year>2003</Year>
<Month>10</Month>
<Day>31</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>11</Month>
<Day>05</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1471-2199</ISSN>
<JournalIssue CitedMedium="Print"><Volume>2</Volume>
<PubDate><Year>2001</Year>
<Month>Nov</Month>
<Day>16</Day>
</PubDate>
</JournalIssue>
<Title>BMC molecular biology</Title>
<ISOAbbreviation>BMC Mol. Biol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Expression of c-myc is not critical for cell proliferation in established human leukemia lines.</ArticleTitle>
<Pagination><MedlinePgn>13</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">A study was undertaken to resolve preliminary conflicting results on the proliferation of leukemia cells observed with different c-myc antisense oligonucleotides.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">RNase H-active, chimeric methylphosphonodiester / phosphodiester antisense oligodeoxynucleotides targeting bases 1147-1166 of c-myc mRNA downregulated c-Myc protein and induced apoptosis and cell cycle arrest respectively in cultures of MOLT-4 and KYO1 human leukemia cells. In contrast, an RNase H-inactive, morpholino antisense oligonucleotide analogue 28-mer, simultaneously targeting the exon 2 splice acceptor site and initiation codon, reduced c-Myc protein to barely detectable levels but did not affect cell proliferation in these or other leukemia lines. The RNase H-active oligodeoxynucleotide 20-mers contained the phosphodiester linked motif CGTTG, which as an apoptosis inducing CpG oligodeoxynucleotide 5-mer of sequence type CGNNN (N = A, G, C, or T) had potent activity against MOLT-4 cells. The 5-mer mimicked the antiproliferative effects of the 20-mer in the absence of any antisense activity against c-myc mRNA, while the latter still reduced expression of c-myc in a subline of MOLT-4 cells that had been selected for resistance to CGTTA, but in this case the oligodeoxynucleotide failed to induce apoptosis or cell cycle arrest.</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">We conclude that the biological activity of the chimeric c-myc antisense 20-mers resulted from a non-antisense mechanism related to the CGTTG motif contained within the sequence, and not through downregulation of c-myc. Although the oncogene may have been implicated in the etiology of the original leukemias, expression of c-myc is apparently no longer required to sustain continuous cell proliferation in these culture lines.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tidd</LastName>
<ForeName>D M</ForeName>
<Initials>DM</Initials>
<AffiliationInfo><Affiliation>School of Biological Sciences, The University of Liverpool, Liverpool, UK. dmtidd@liv.ac.uk</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Giles</LastName>
<ForeName>R V</ForeName>
<Initials>RV</Initials>
</Author>
<Author ValidYN="Y"><LastName>Broughton</LastName>
<ForeName>C M</ForeName>
<Initials>CM</Initials>
</Author>
<Author ValidYN="Y"><LastName>Clark</LastName>
<ForeName>R E</ForeName>
<Initials>RE</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2001</Year>
<Month>11</Month>
<Day>16</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>BMC Mol Biol</MedlineTA>
<NlmUniqueID>100966983</NlmUniqueID>
<ISSNLinking>1471-2199</ISSNLinking>
</MedlineJournalInfo>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2001</Year>
<Month>09</Month>
<Day>26</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2001</Year>
<Month>11</Month>
<Day>16</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2001</Year>
<Month>12</Month>
<Day>6</Day>
<Hour>10</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2001</Year>
<Month>12</Month>
<Day>6</Day>
<Hour>10</Hour>
<Minute>1</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2001</Year>
<Month>12</Month>
<Day>6</Day>
<Hour>10</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">11734062</ArticleId>
<ArticleId IdType="pmc">PMC60647</ArticleId>
<ArticleId IdType="doi">10.1186/1471-2199-2-13</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Biochem J. 1996 Mar 15;314 ( Pt 3):713-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8615760</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antisense Res Dev. 1995 Spring;5(1):23-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7542045</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Biol. 1997 Mar;17(3):1459-68</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9032273</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1998 Apr 1;26(7):1567-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9512525</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Physiol. 1998;60:575-600</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9558477</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FASEB J. 1998 Jun;12(9):633-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9619443</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Blood. 1998 Jun 15;91(12):4738-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9616172</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antisense Nucleic Acid Drug Dev. 1998 Aug;8(4):281-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9743466</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antisense Nucleic Acid Drug Dev. 1999 Apr;9(2):213-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10355827</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Oncogene. 2000 Jan 6;19(1):115-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10644986</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Carcinogenesis. 2000 Mar;21(3):485-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10688869</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Oncogene. 2000 Feb 21;19(8):1065-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10713691</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2000 Jun 1;28(11):2242-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10871345</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Apoptosis. 2001 Feb-Apr;6(1-2):133-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11321036</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 1982 Jun;42(3):773-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6284994</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1982 Sep 2;299(5878):61-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6955596</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cancer Res. 1986 Jul;46(7):3211-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3011242</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1987 Jan 16;235(4786):305-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3541204</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1987 Jul 30-Aug 5;328(6129):445-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3302722</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1988 Feb;85(4):1028-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3277186</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anticancer Res. 1990 Sep-Oct;10(5A):1169-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2241097</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1992 Feb 25;20(4):763-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1371864</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anticancer Drug Des. 1992 Feb;7(1):37-48</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1311929</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 1993 Jun 15;150(12):5418-28</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8515068</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Soc Trans. 1996 Aug;24(3):410S</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8878954</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002528 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 002528 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= PubMed |étape= Curation |type= RBID |clé= pubmed:11734062 |texte= Expression of c-myc is not critical for cell proliferation in established human leukemia lines. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i -Sk "pubmed:11734062" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |